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The map label for this gene is fmt

Identifier: 123966150

GI number: 123966150

Start: 819624

End: 820610

Strand: Reverse

Name: fmt

Synonym: P9515_09171

Alternate gene names: 123966150

Gene position: 820610-819624 (Counterclockwise)

Preceding gene: 123966151

Following gene: 123966148

Centisome position: 48.15

GC content: 27.56

Gene sequence:

>987_bases
GTGAGAATTATATTTTGGGGAACTCCTGAATATTCAGTAAAAAGTCTTGAAGTATTAAAAAAATCAGATCATGAAATTTT
AGCTGTCATAACGCAGCCAGATAAAAAAAGATCTAGAGGTAATAAATTAATAGCGTCCCCAGTAAAGCAATATGCTATGA
AGGAAGGGCTTCCAGTTTTCACACCTGAAACTTTAAAGAAAAATGATCATTTTATAAGTTTACTTAAAGAATTTTCTTGC
GATTTGTTTGTTGTTATTGCTTATGGAAAAATATTACCAAAAAAAATATTAGATATTCCTAAATATAAATCATGGAATGC
GCATGCATCACTACTTCCAAGATGGAGAGGTGCCGCTCCCATCCAATGGTCAATATTGGAGGGAGATGATTTTACTGGTG
TTGGAATAATGAGAATGGAAGAAGGATTAGATACTGGAGATGTATTAGTTGAAAAACAAATCAAAATTGAGAAAGAAGAT
AATCTTCAGACGTTAACAAAAAAATTAAGTGATTTATCATCAGAATTATTATTAAAAGCGATATCAAAAATAGAGCAAAA
TAAAAATAAAGATATTAATCATTTACTTAAAAAACAAAAAGATCTTCAAAGAAAATTAAAATATGCAAGAATGATTAACA
AATCAGACTACATAATAGATTGGGCAAATAATTCAACTGATATATACAGAAAAATAAATGCCTTATACCCTAGAGTTAGT
ACAACATTTAAAAAGAAAAATCTAAAAATAATTAAAATAAAAATTCTAACAACAGATGAGATACAGAATAAAAATTATAA
AATTGTAAGCGATAAATTTAAACCTGGATACGTTATTGGTCTTATAGAAAACAAAGGTATCATTATATCAACTAAAACTG
ATCCAATTCTTTTATTAGAGGCAAAACTAGAGGGTAAAAATATATCTAAGCAAAAGCAGCTTTTACAACAATTAAATCCT
CTTATAGGAGAAAAATTCTCAGATTAA

Upstream 100 bases:

>100_bases
TTCTTAGAAATATAGTTAATATTGAAAATGAAGAAGAATTAACTACAAGTGGAATTTCCCCACATATTTGGATTAGTGAA
TTATCAATAACTGGAGACGA

Downstream 100 bases:

>100_bases
GTTATTATCTCCCTTTTGGACAAACCCCAAAGGAAGGAGATTAATATCAAAATATAAAAATAATAATCAGGAAGAATTGT
CTCTGTAATTAATGTATTTA

Product: putative methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRIIFWGTPEYSVKSLEVLKKSDHEILAVITQPDKKRSRGNKLIASPVKQYAMKEGLPVFTPETLKKNDHFISLLKEFSC
DLFVVIAYGKILPKKILDIPKYKSWNAHASLLPRWRGAAPIQWSILEGDDFTGVGIMRMEEGLDTGDVLVEKQIKIEKED
NLQTLTKKLSDLSSELLLKAISKIEQNKNKDINHLLKKQKDLQRKLKYARMINKSDYIIDWANNSTDIYRKINALYPRVS
TTFKKKNLKIIKIKILTTDEIQNKNYKIVSDKFKPGYVIGLIENKGIIISTKTDPILLLEAKLEGKNISKQKQLLQQLNP
LIGEKFSD

Sequences:

>Translated_328_residues
MRIIFWGTPEYSVKSLEVLKKSDHEILAVITQPDKKRSRGNKLIASPVKQYAMKEGLPVFTPETLKKNDHFISLLKEFSC
DLFVVIAYGKILPKKILDIPKYKSWNAHASLLPRWRGAAPIQWSILEGDDFTGVGIMRMEEGLDTGDVLVEKQIKIEKED
NLQTLTKKLSDLSSELLLKAISKIEQNKNKDINHLLKKQKDLQRKLKYARMINKSDYIIDWANNSTDIYRKINALYPRVS
TTFKKKNLKIIKIKILTTDEIQNKNYKIVSDKFKPGYVIGLIENKGIIISTKTDPILLLEAKLEGKNISKQKQLLQQLNP
LIGEKFSD
>Mature_328_residues
MRIIFWGTPEYSVKSLEVLKKSDHEILAVITQPDKKRSRGNKLIASPVKQYAMKEGLPVFTPETLKKNDHFISLLKEFSC
DLFVVIAYGKILPKKILDIPKYKSWNAHASLLPRWRGAAPIQWSILEGDDFTGVGIMRMEEGLDTGDVLVEKQIKIEKED
NLQTLTKKLSDLSSELLLKAISKIEQNKNKDINHLLKKQKDLQRKLKYARMINKSDYIIDWANNSTDIYRKINALYPRVS
TTFKKKNLKIIKIKILTTDEIQNKNYKIVSDKFKPGYVIGLIENKGIIISTKTDPILLLEAKLEGKNISKQKQLLQQLNP
LIGEKFSD

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=291, Percent_Identity=25.085910652921, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI21614513, Length=306, Percent_Identity=24.1830065359477, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI238814322, Length=155, Percent_Identity=30.9677419354839, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1789683, Length=317, Percent_Identity=35.3312302839117, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1788589, Length=235, Percent_Identity=23.4042553191489, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI133930964, Length=179, Percent_Identity=34.0782122905028, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550868, Length=258, Percent_Identity=32.9457364341085, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI28571984, Length=215, Percent_Identity=33.4883720930233, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585660, Length=176, Percent_Identity=31.8181818181818, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_PROM5 (A2BWG3)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011231.1
- ProteinModelPortal:   A2BWG3
- SMR:   A2BWG3
- STRING:   A2BWG3
- GeneID:   4719325
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_09171
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   WRGAGPI
- ProtClustDB:   CLSK921992
- BioCyc:   PMAR167542:P9515ORF_0962-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 37693; Mature: 37693

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIFWGTPEYSVKSLEVLKKSDHEILAVITQPDKKRSRGNKLIASPVKQYAMKEGLPVF
CEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCC
TPETLKKNDHFISLLKEFSCDLFVVIAYGKILPKKILDIPKYKSWNAHASLLPRWRGAAP
CHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCC
IQWSILEGDDFTGVGIMRMEEGLDTGDVLVEKQIKIEKEDNLQTLTKKLSDLSSELLLKA
EEEEEECCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ISKIEQNKNKDINHLLKKQKDLQRKLKYARMINKSDYIIDWANNSTDIYRKINALYPRVS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCHH
TTFKKKNLKIIKIKILTTDEIQNKNYKIVSDKFKPGYVIGLIENKGIIISTKTDPILLLE
HHHHHCCEEEEEEEEEEECCCCCCCEEEEECCCCCCEEEEEEECCCEEEEECCCCEEEEE
AKLEGKNISKQKQLLQQLNPLIGEKFSD
EECCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRIIFWGTPEYSVKSLEVLKKSDHEILAVITQPDKKRSRGNKLIASPVKQYAMKEGLPVF
CEEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCC
TPETLKKNDHFISLLKEFSCDLFVVIAYGKILPKKILDIPKYKSWNAHASLLPRWRGAAP
CHHHHCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCC
IQWSILEGDDFTGVGIMRMEEGLDTGDVLVEKQIKIEKEDNLQTLTKKLSDLSSELLLKA
EEEEEECCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ISKIEQNKNKDINHLLKKQKDLQRKLKYARMINKSDYIIDWANNSTDIYRKINALYPRVS
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCHH
TTFKKKNLKIIKIKILTTDEIQNKNYKIVSDKFKPGYVIGLIENKGIIISTKTDPILLLE
HHHHHCCEEEEEEEEEEECCCCCCCEEEEECCCCCCEEEEEEECCCEEEEECCCCEEEEE
AKLEGKNISKQKQLLQQLNPLIGEKFSD
EECCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA