Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 123966137

GI number: 123966137

Start: 804917

End: 805642

Strand: Direct

Name: tpiA

Synonym: P9515_09041

Alternate gene names: 123966137

Gene position: 804917-805642 (Clockwise)

Preceding gene: 123966136

Following gene: 123966138

Centisome position: 47.23

GC content: 34.71

Gene sequence:

>726_bases
TTGAGAAAATCTGTAATCGCCGGTAATTGGAAGATGCACATGACTTGTGCTGAAACAAAAAATTATTTGGAGGAATTTAT
TCCTCTTATAAAAGACCTTCCTAATGATAGGAAGATTGTTATTGCCCCTCCATTTACAGCAATTTCAACTTTTTCGAGTT
ACTCTAACTTTGATTATTTAGATATTGCAAGCCAAAACATTCACTGGGAAGATCAAGGTGCTTTCACAGCAGAAATTTCT
CCAAAAATGCTTATTGAACACAATGTTAAATATGCAATTGTTGGACATAGTGAACCTAGGAAATATTTCAGTGAAAGTGA
TGAACAAATTAATAAGAGAGCAGTATTTGCTCAATCAAGTGGTCTCACTCCAATAGTCTGTGTTGGTGAAACTCTTGAAC
AAAGAGAAAGAGGAGAAGCTAATAGAGTCATCACAAGACAAGTTGAACAAGGATTGGAGAATACTGATCCATCTAATCTC
ATAATTGCTTATGAACCTATATGGGCGATTGGTACCGGTAAAACTTGTGAAGCAAGTGATGCCAATAAAATATGTGCTTT
GATAAGGGAGTTAATAGGTTTTGATGATGTAATTATTCAATATGGAGGTTCTGTTAAGCCTAATAATATTGACGAGATTA
TGTCGATGAGCGATATTAATGGAGTATTGGTAGGGGGCTCTTCTCTAGATCCAATTAGTTTTTCAAGAATTGCAAATTAC
GAATAA

Upstream 100 bases:

>100_bases
AAAGGAGATAAAGTTATGTTTCTAAAAAATGAATTAATTTTTGAATAATTAGCCTATTAGCTGCAAGTTATATTAGTATT
ATTTTCTTGGACAATATATT

Downstream 100 bases:

>100_bases
TAATTATCCTTGGCCTGATTATTGGGGACGAAAAACTTCAATAATGGGGATTATAAATTTAACTCCCGATTCATTTAGTG
ATGGAGGTGAATTTTGCTCA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MRKSVIAGNWKMHMTCAETKNYLEEFIPLIKDLPNDRKIVIAPPFTAISTFSSYSNFDYLDIASQNIHWEDQGAFTAEIS
PKMLIEHNVKYAIVGHSEPRKYFSESDEQINKRAVFAQSSGLTPIVCVGETLEQRERGEANRVITRQVEQGLENTDPSNL
IIAYEPIWAIGTGKTCEASDANKICALIRELIGFDDVIIQYGGSVKPNNIDEIMSMSDINGVLVGGSSLDPISFSRIANY
E

Sequences:

>Translated_241_residues
MRKSVIAGNWKMHMTCAETKNYLEEFIPLIKDLPNDRKIVIAPPFTAISTFSSYSNFDYLDIASQNIHWEDQGAFTAEIS
PKMLIEHNVKYAIVGHSEPRKYFSESDEQINKRAVFAQSSGLTPIVCVGETLEQRERGEANRVITRQVEQGLENTDPSNL
IIAYEPIWAIGTGKTCEASDANKICALIRELIGFDDVIIQYGGSVKPNNIDEIMSMSDINGVLVGGSSLDPISFSRIANY
E
>Mature_241_residues
MRKSVIAGNWKMHMTCAETKNYLEEFIPLIKDLPNDRKIVIAPPFTAISTFSSYSNFDYLDIASQNIHWEDQGAFTAEIS
PKMLIEHNVKYAIVGHSEPRKYFSESDEQINKRAVFAQSSGLTPIVCVGETLEQRERGEANRVITRQVEQGLENTDPSNL
IIAYEPIWAIGTGKTCEASDANKICALIRELIGFDDVIIQYGGSVKPNNIDEIMSMSDINGVLVGGSSLDPISFSRIANY
E

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=240, Percent_Identity=37.9166666666667, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI4507645, Length=240, Percent_Identity=37.9166666666667, Blast_Score=152, Evalue=3e-37,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=36.9477911646586, Blast_Score=160, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17536593, Length=246, Percent_Identity=37.3983739837398, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320255, Length=238, Percent_Identity=34.453781512605, Blast_Score=141, Evalue=7e-35,
Organism=Drosophila melanogaster, GI28572008, Length=241, Percent_Identity=38.1742738589212, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI28572006, Length=241, Percent_Identity=38.1742738589212, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI28572004, Length=241, Percent_Identity=38.1742738589212, Blast_Score=150, Evalue=9e-37,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PROM5 (A2BWF0)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011218.1
- ProteinModelPortal:   A2BWF0
- SMR:   A2BWF0
- STRING:   A2BWF0
- GeneID:   4719312
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_09041
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   PMAR167542:P9515ORF_0950-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26872; Mature: 26872

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 165-165 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSVIAGNWKMHMTCAETKNYLEEFIPLIKDLPNDRKIVIAPPFTAISTFSSYSNFDYL
CCCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCEE
DIASQNIHWEDQGAFTAEISPKMLIEHNVKYAIVGHSEPRKYFSESDEQINKRAVFAQSS
EECCCCCCCCCCCCEEEECCCEEEEECCCEEEEEECCCHHHHHCCCHHHHHHHEEEECCC
GLTPIVCVGETLEQRERGEANRVITRQVEQGLENTDPSNLIIAYEPIWAIGTGKTCEASD
CCCEEEECCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCC
ANKICALIRELIGFDDVIIQYGGSVKPNNIDEIMSMSDINGVLVGGSSLDPISFSRIANY
HHHHHHHHHHHHCCCCEEHCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHCCC
E
C
>Mature Secondary Structure
MRKSVIAGNWKMHMTCAETKNYLEEFIPLIKDLPNDRKIVIAPPFTAISTFSSYSNFDYL
CCCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCEE
DIASQNIHWEDQGAFTAEISPKMLIEHNVKYAIVGHSEPRKYFSESDEQINKRAVFAQSS
EECCCCCCCCCCCCEEEECCCEEEEECCCEEEEEECCCHHHHHCCCHHHHHHHEEEECCC
GLTPIVCVGETLEQRERGEANRVITRQVEQGLENTDPSNLIIAYEPIWAIGTGKTCEASD
CCCEEEECCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCC
ANKICALIRELIGFDDVIIQYGGSVKPNNIDEIMSMSDINGVLVGGSSLDPISFSRIANY
HHHHHHHHHHHHCCCCEEHCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCHHHHCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA