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The map label for this gene is galE [C]

Identifier: 123965997

GI number: 123965997

Start: 692699

End: 693562

Strand: Direct

Name: galE [C]

Synonym: P9515_07621

Alternate gene names: 123965997

Gene position: 692699-693562 (Clockwise)

Preceding gene: 123965996

Following gene: 123965998

Centisome position: 40.65

GC content: 27.89

Gene sequence:

>864_bases
ATGAAATTATTTATAACTGGTGCGAATGGGACTATTGGCTCTGAATTATGCAAAATTTGTTATTCAAATAAAATTTTCAA
TAAAATATTTCTTAGAAAAAATAGTATATGTAATCATAAACTGTATAAAGAAAAGTTAGTTCTTGATGATATATCTGAGG
TTAATTTTGAAGAATTAAGAGGAACTGATGTTTTTGTCCATCTTGCTTCAGCTGGAGTTGATCAGACTAAAGAAGCTGAT
CCTAATGAAATTTTTGATTTTAATGTTTTAAAGTCATTAGAATTACTTTTAAAAGCAATAAAGGCAGGGGTAAAAAAATT
CATCATAGTAGGTAGTTTTTTTGAATACGGGAAAACAGCTAATTTAACAAATAAAGATATTTCAGTTACAGATCCATTAA
TACCAACGAGTATTTATGGGGCAAGCAAAGCTGCATTTACTAGTGCCTGTATTGGGCTAGCGTATTCATATGGTGTATCC
GTTTATATTCTTAGACCGTTTTATGTATATGGCGAAAACGAATCTAAAAACAGACTATATAAAAGCTTATTAAGTAAAAT
AAAAAGTGGAGAAGATTTTCATTTAACTGAAGGCTCTCAAATTAGAGATTTTTCAAGTAGTGAAGAAATGGCAACTTTGA
TTTTTGAAGAGATATTTAAAATTTACAAAGAAAATGAAAAGAAACTTAAAATAAAAAATCTAGGTAGCGGAAGATCAATG
TCAATTTATGAATTCGCTAAAAAAATTTGGATAGAAAATAAAGCAAAAGGAAAACTAATAAAAGGTTCTTTGCCCTATAG
AAATAATGAATCAATGCGAAGTGTGCCTAATCTTGAACTAATTTTTGAAGATGATATTAAATAA

Upstream 100 bases:

>100_bases
AATGCTTGAATAATATAAATTCTTTTCAAACTGAAAATTAATTTATTAAAGTCTTTAATTTTTATAAATTAGAATTATAG
ATAAATTAATAATTTATCCT

Downstream 100 bases:

>100_bases
TATTTATTATTTTGAAATAATTAAATTTCATTTTTCTAAAGTTTTGAAAACAAGATTTTTCAGAATTTACTCTACTAAGT
TAGAAATTTAGACAAAATTA

Product: nucleoside-diphosphate-sugar epimerase

Products: UDPglucoseal [C]

Alternate protein names: Nucleoside-Diphosphate-Sugar Epimerase; UDP-Glucose 4-Epimerase; NAD-Dependent Epimerase/Dehydratase Family Protein; Nucleotide Sugar Epimerase/Dehydratase; CDP-Abequose Synthase; NAD Dependent Epimerase/Dehydratase Family; Nucleoside-Diphosphate-Sugar Epimerase Family Protein

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MKLFITGANGTIGSELCKICYSNKIFNKIFLRKNSICNHKLYKEKLVLDDISEVNFEELRGTDVFVHLASAGVDQTKEAD
PNEIFDFNVLKSLELLLKAIKAGVKKFIIVGSFFEYGKTANLTNKDISVTDPLIPTSIYGASKAAFTSACIGLAYSYGVS
VYILRPFYVYGENESKNRLYKSLLSKIKSGEDFHLTEGSQIRDFSSSEEMATLIFEEIFKIYKENEKKLKIKNLGSGRSM
SIYEFAKKIWIENKAKGKLIKGSLPYRNNESMRSVPNLELIFEDDIK

Sequences:

>Translated_287_residues
MKLFITGANGTIGSELCKICYSNKIFNKIFLRKNSICNHKLYKEKLVLDDISEVNFEELRGTDVFVHLASAGVDQTKEAD
PNEIFDFNVLKSLELLLKAIKAGVKKFIIVGSFFEYGKTANLTNKDISVTDPLIPTSIYGASKAAFTSACIGLAYSYGVS
VYILRPFYVYGENESKNRLYKSLLSKIKSGEDFHLTEGSQIRDFSSSEEMATLIFEEIFKIYKENEKKLKIKNLGSGRSM
SIYEFAKKIWIENKAKGKLIKGSLPYRNNESMRSVPNLELIFEDDIK
>Mature_287_residues
MKLFITGANGTIGSELCKICYSNKIFNKIFLRKNSICNHKLYKEKLVLDDISEVNFEELRGTDVFVHLASAGVDQTKEAD
PNEIFDFNVLKSLELLLKAIKAGVKKFIIVGSFFEYGKTANLTNKDISVTDPLIPTSIYGASKAAFTSACIGLAYSYGVS
VYILRPFYVYGENESKNRLYKSLLSKIKSGEDFHLTEGSQIRDFSSSEEMATLIFEEIFKIYKENEKKLKIKNLGSGRSM
SIYEFAKKIWIENKAKGKLIKGSLPYRNNESMRSVPNLELIFEDDIK

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 32486; Mature: 32486

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFITGANGTIGSELCKICYSNKIFNKIFLRKNSICNHKLYKEKLVLDDISEVNFEELR
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHC
GTDVFVHLASAGVDQTKEADPNEIFDFNVLKSLELLLKAIKAGVKKFIIVGSFFEYGKTA
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHCCCCC
NLTNKDISVTDPLIPTSIYGASKAAFTSACIGLAYSYGVSVYILRPFYVYGENESKNRLY
CCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHHHHH
KSLLSKIKSGEDFHLTEGSQIRDFSSSEEMATLIFEEIFKIYKENEKKLKIKNLGSGRSM
HHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
SIYEFAKKIWIENKAKGKLIKGSLPYRNNESMRSVPNLELIFEDDIK
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHCCCCEEEEEECCCC
>Mature Secondary Structure
MKLFITGANGTIGSELCKICYSNKIFNKIFLRKNSICNHKLYKEKLVLDDISEVNFEELR
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHC
GTDVFVHLASAGVDQTKEADPNEIFDFNVLKSLELLLKAIKAGVKKFIIVGSFFEYGKTA
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHCCCCC
NLTNKDISVTDPLIPTSIYGASKAAFTSACIGLAYSYGVSVYILRPFYVYGENESKNRLY
CCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHHHHH
KSLLSKIKSGEDFHLTEGSQIRDFSSSEEMATLIFEEIFKIYKENEKKLKIKNLGSGRSM
HHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
SIYEFAKKIWIENKAKGKLIKGSLPYRNNESMRSVPNLELIFEDDIK
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA