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The map label for this gene is met17 [H]

Identifier: 123965943

GI number: 123965943

Start: 642024

End: 643352

Strand: Direct

Name: met17 [H]

Synonym: P9515_07081

Alternate gene names: 123965943

Gene position: 642024-643352 (Clockwise)

Preceding gene: 123965942

Following gene: 123965944

Centisome position: 37.67

GC content: 37.77

Gene sequence:

>1329_bases
TTGAGTAACCAAAAGTTCGAGACTCTTCAGTTACATGCAGGCCAAGAGCCTGATCCAACTACTAACTCCAGAGCTGTACC
TATCTATCAGACAAGTTCTTACGTTTTTGATAACGCAGAACATGGAGCTAATCTTTTTGGATTAAAAGAATTTGGAAATA
TCTATACAAGACTCATGAACCCCACAACTGATGTCTTCGAAAAAAGGATGGCAGCTTTAGAAGGCGGTATGGCTGCATTG
GCAACTTCATCAGGTCAAGCTGCTCAATTCCTTGCAATAGTCAATTGCATGAAAGCAGGGGATAATTTTGTTTCTACTTC
CTTTTTGTATGGTGGAACTTTTAATCAATTTAAAGTTCAATTCCCTAGGTTGGGAATAGAAGTTAAATTTGCTGAGGGAG
ATAGTGTTGATAGTTTTAAAGAGAAAATAGATGATAAAACTAAAGCAATTTATGTTGAATCAATGGGAAATCCTAGATTC
AACATACCCGATTTTGAGGGCCTTTCTAATTTGGCTAAGGAAAATGGTATTCCTTTAATTGTTGACAATACTCTTGGTGC
AGGAGGCGCTTTAATTAAACCAATTGATTTTGGTGCAGATATTGTTGTAGAAAGCGCAACTAAATGGATAGGAGGACATG
GAACTAGTATCGGAGGAGTAATAGTTGATGCTGGAACTTTTGATTGGGGAAATGGAAAATTCCCACTTATGAGTGAACCA
AGCGCTGCCTATCATGGTTTGGTACATTGGGATGCATTTGGATTTGGGAGTGATATATGTAAATCTTTAGGTGTCCCTGA
CAATAGAAATATAGCTTTTGCCTTGAGAGCTAGGCTGGAGTGTCTAAGAGATTGGGGCCCGGCTCAAAGTCCTTTTAATT
CTTTTTTATTATTACAAGGCTTAGAAACTTTAAGTTTAAGAATAGAGAGACAAACTTCTAACGCGCTTGAGTTGGCAAAA
TGGTTAGATTCAAACCCTCAAGTTACCAGCGTTAATTTCCCTGGTTTGGAATCCGATCCCTATCATACAAGAGCAAAAAA
ATACACTACAGGCAGAGGTATGGGATGTATGCTTATGTTCTCTTTAAATGGCGGATATGACAACGCCGTTAAATTTATTG
ACTCATTAGAATTAGCTAGTCATCTTGCTAATGTTGGAGACTCTAAAACTTTAGTTATTCACCCTGCCTCCACAACACAT
CAGCAATTATCAGAGGAAGAACAATTATCAGCAGGTGTTACTCCAACAATGGTAAGAGTATCTGTTGGGATAGAGCATAT
TGATGATATAAAAGCAGATTTTGAACAGGCGCTTACAAAAATTACTTAA

Upstream 100 bases:

>100_bases
TTATCCATCATTAAAAGAGGATTTATAAAGATTTATCTTGTTTATATTTATTTTTAGGAAGTTTTTAAGTTAAATAGATA
ATAGGATTAATTAAAAAATT

Downstream 100 bases:

>100_bases
ATAATGGAGATTCATTGGCTTTAATAATACCTAGCAATTATCACAAGATTAGTGATGTTGAGAAAAATCATATTTCTTGG
ATTGAACCGGAATTGGCAGA

Product: putative O-Acetyl homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSNQKFETLQLHAGQEPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAAL
ATSSGQAAQFLAIVNCMKAGDNFVSTSFLYGGTFNQFKVQFPRLGIEVKFAEGDSVDSFKEKIDDKTKAIYVESMGNPRF
NIPDFEGLSNLAKENGIPLIVDNTLGAGGALIKPIDFGADIVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEP
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGPAQSPFNSFLLLQGLETLSLRIERQTSNALELAK
WLDSNPQVTSVNFPGLESDPYHTRAKKYTTGRGMGCMLMFSLNGGYDNAVKFIDSLELASHLANVGDSKTLVIHPASTTH
QQLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALTKIT

Sequences:

>Translated_442_residues
MSNQKFETLQLHAGQEPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAAL
ATSSGQAAQFLAIVNCMKAGDNFVSTSFLYGGTFNQFKVQFPRLGIEVKFAEGDSVDSFKEKIDDKTKAIYVESMGNPRF
NIPDFEGLSNLAKENGIPLIVDNTLGAGGALIKPIDFGADIVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEP
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGPAQSPFNSFLLLQGLETLSLRIERQTSNALELAK
WLDSNPQVTSVNFPGLESDPYHTRAKKYTTGRGMGCMLMFSLNGGYDNAVKFIDSLELASHLANVGDSKTLVIHPASTTH
QQLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALTKIT
>Mature_441_residues
SNQKFETLQLHAGQEPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAALA
TSSGQAAQFLAIVNCMKAGDNFVSTSFLYGGTFNQFKVQFPRLGIEVKFAEGDSVDSFKEKIDDKTKAIYVESMGNPRFN
IPDFEGLSNLAKENGIPLIVDNTLGAGGALIKPIDFGADIVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEPS
AAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGPAQSPFNSFLLLQGLETLSLRIERQTSNALELAKW
LDSNPQVTSVNFPGLESDPYHTRAKKYTTGRGMGCMLMFSLNGGYDNAVKFIDSLELASHLANVGDSKTLVIHPASTTHQ
QLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALTKIT

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=439, Percent_Identity=29.8405466970387, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI299473759, Length=439, Percent_Identity=26.879271070615, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI25453487, Length=438, Percent_Identity=26.9406392694064, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1790375, Length=433, Percent_Identity=28.8683602771363, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789383, Length=448, Percent_Identity=22.3214285714286, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71980708, Length=460, Percent_Identity=30.2173913043478, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=438, Percent_Identity=31.9634703196347, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=437, Percent_Identity=30.8924485125858, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=450, Percent_Identity=53.3333333333333, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319307, Length=445, Percent_Identity=30.561797752809, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321254, Length=464, Percent_Identity=24.7844827586207, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321170, Length=334, Percent_Identity=25.4491017964072, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI19922554, Length=438, Percent_Identity=29.2237442922374, Blast_Score=169, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 48002; Mature: 47870

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQKFETLQLHAGQEPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMN
CCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEEECCCCCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRMAALEGGMAALATSSGQAAQFLAIVNCMKAGDNFVSTSFLYGGTFNQFKVQ
CHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEE
FPRLGIEVKFAEGDSVDSFKEKIDDKTKAIYVESMGNPRFNIPDFEGLSNLAKENGIPLI
ECCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEE
VDNTLGAGGALIKPIDFGADIVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEP
EECCCCCCCCEEEEECCCCCEEEEHHHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGPAQSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHC
LETLSLRIERQTSNALELAKWLDSNPQVTSVNFPGLESDPYHTRAKKYTTGRGMGCMLMF
CCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCEEEEEE
SLNGGYDNAVKFIDSLELASHLANVGDSKTLVIHPASTTHQQLSEEEQLSAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCHHHHHHCCCCCEEEEE
SVGIEHIDDIKADFEQALTKIT
EECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNQKFETLQLHAGQEPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMN
CCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEEECCCCCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRMAALEGGMAALATSSGQAAQFLAIVNCMKAGDNFVSTSFLYGGTFNQFKVQ
CHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEE
FPRLGIEVKFAEGDSVDSFKEKIDDKTKAIYVESMGNPRFNIPDFEGLSNLAKENGIPLI
ECCCCEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEE
VDNTLGAGGALIKPIDFGADIVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSEP
EECCCCCCCCEEEEECCCCCEEEEHHHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGPAQSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHC
LETLSLRIERQTSNALELAKWLDSNPQVTSVNFPGLESDPYHTRAKKYTTGRGMGCMLMF
CCEEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCEEEEEE
SLNGGYDNAVKFIDSLELASHLANVGDSKTLVIHPASTTHQQLSEEEQLSAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCHHHHHHCCCCCEEEEE
SVGIEHIDDIKADFEQALTKIT
EECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]