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The map label for this gene is gloB [H]

Identifier: 123965858

GI number: 123965858

Start: 556977

End: 557717

Strand: Reverse

Name: gloB [H]

Synonym: P9515_06231

Alternate gene names: 123965858

Gene position: 557717-556977 (Counterclockwise)

Preceding gene: 123965862

Following gene: 123965857

Centisome position: 32.73

GC content: 29.96

Gene sequence:

>741_bases
ATGGAATTTAATAAAGAGCAAACAATAATTGGAATTAGAGTTCTCAGTGATAATGTCATATGGTTATGGAGAAAGAATAA
TTCAGCAGTAGTAATTGATCCTGCCCTTTCTCAACCTGTAATAGAATACCTAAAAACCCATAATCTTTATTTAAAAGCTA
TTTTACAAACACATCATCACCACGATCATATTGGAGGGACAAGAGAACTTATAAAAGAATGGCCTAAGGTTAAAGTTATA
GCTTCAGCAAAAGAAAAAGATCGAATACCATTTCAAAATTTATCTGTCAAAGATGGAGACAAGTTAAAATTATTAGACGA
GGAAGTTCACGTGATAGAAGTAATAGGGCATACAAGATCACACCTGGCTTTCTACATTCATAATAAAGTTCCAATTCTTT
TTATTGGTGATACGTTATTTTCAGCTGGATGTGGAAGAATATTTGAAGGAACTTACAAACAAATGTATAACTCACTTAAA
AAAATAAAGTCATTACCTAAAAACACCACTATTTACTGTGCTCATGAGTATACAAAGTCAAATCTTTTATGGGCCTTAGA
TCTTGAACCTGAAAACGAAAATATAAAAAATAAACTAATTGAAGTTGAAAAGAAAATTGCGTTAAAAGAACTTACAATTC
CATTTCTACTCGAAGAAGAGTTGATAATTAATCTGTTTTTAAGAGCAGATAATTTAAAAAGATTTTCTTACTTAAGAGCA
AATAAAGATACATGGGTTTAA

Upstream 100 bases:

>100_bases
TTTTAAAAATTGAAATTGAATCCTCTAACAGAGCTCCTTTGGGTAAAGCTATAGTAATCATAGATCAATTTTTACGCAAT
CTTTTAATTCTAAGGTAATA

Downstream 100 bases:

>100_bases
ATTTATAGAAACATCTTTCATCAAATGTCCTCATCAAAAGTAATTAAGACTTCTAATGCCCCAGACCCAGTTGGTCCTTA
TAACCAAGCTATAAAAGCAG

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MEFNKEQTIIGIRVLSDNVIWLWRKNNSAVVIDPALSQPVIEYLKTHNLYLKAILQTHHHHDHIGGTRELIKEWPKVKVI
ASAKEKDRIPFQNLSVKDGDKLKLLDEEVHVIEVIGHTRSHLAFYIHNKVPILFIGDTLFSAGCGRIFEGTYKQMYNSLK
KIKSLPKNTTIYCAHEYTKSNLLWALDLEPENENIKNKLIEVEKKIALKELTIPFLLEEELIINLFLRADNLKRFSYLRA
NKDTWV

Sequences:

>Translated_246_residues
MEFNKEQTIIGIRVLSDNVIWLWRKNNSAVVIDPALSQPVIEYLKTHNLYLKAILQTHHHHDHIGGTRELIKEWPKVKVI
ASAKEKDRIPFQNLSVKDGDKLKLLDEEVHVIEVIGHTRSHLAFYIHNKVPILFIGDTLFSAGCGRIFEGTYKQMYNSLK
KIKSLPKNTTIYCAHEYTKSNLLWALDLEPENENIKNKLIEVEKKIALKELTIPFLLEEELIINLFLRADNLKRFSYLRA
NKDTWV
>Mature_246_residues
MEFNKEQTIIGIRVLSDNVIWLWRKNNSAVVIDPALSQPVIEYLKTHNLYLKAILQTHHHHDHIGGTRELIKEWPKVKVI
ASAKEKDRIPFQNLSVKDGDKLKLLDEEVHVIEVIGHTRSHLAFYIHNKVPILFIGDTLFSAGCGRIFEGTYKQMYNSLK
KIKSLPKNTTIYCAHEYTKSNLLWALDLEPENENIKNKLIEVEKKIALKELTIPFLLEEELIINLFLRADNLKRFSYLRA
NKDTWV

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=222, Percent_Identity=34.2342342342342, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI94538320, Length=222, Percent_Identity=34.2342342342342, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI14150041, Length=223, Percent_Identity=34.5291479820628, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI46361987, Length=194, Percent_Identity=34.5360824742268, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI116642887, Length=230, Percent_Identity=33.9130434782609, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI21703352, Length=230, Percent_Identity=33.9130434782609, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=37.8486055776892, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17536925, Length=223, Percent_Identity=34.0807174887892, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17538952, Length=192, Percent_Identity=29.6875, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320478, Length=239, Percent_Identity=30.9623430962343, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=219, Percent_Identity=30.5936073059361, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356335, Length=252, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24667711, Length=252, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24667703, Length=252, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI221330176, Length=160, Percent_Identity=34.375, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28649; Mature: 28649

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFNKEQTIIGIRVLSDNVIWLWRKNNSAVVIDPALSQPVIEYLKTHNLYLKAILQTHHH
CCCCCCCEEEEEEEECCCEEEEEEECCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCC
HDHIGGTRELIKEWPKVKVIASAKEKDRIPFQNLSVKDGDKLKLLDEEVHVIEVIGHTRS
CCCCCCHHHHHHHCCCEEEEECCCHHCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCC
HLAFYIHNKVPILFIGDTLFSAGCGRIFEGTYKQMYNSLKKIKSLPKNTTIYCAHEYTKS
EEEEEEECCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
NLLWALDLEPENENIKNKLIEVEKKIALKELTIPFLLEEELIINLFLRADNLKRFSYLRA
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHEEEEEECCCCHHHHHEEC
NKDTWV
CCCCCC
>Mature Secondary Structure
MEFNKEQTIIGIRVLSDNVIWLWRKNNSAVVIDPALSQPVIEYLKTHNLYLKAILQTHHH
CCCCCCCEEEEEEEECCCEEEEEEECCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCC
HDHIGGTRELIKEWPKVKVIASAKEKDRIPFQNLSVKDGDKLKLLDEEVHVIEVIGHTRS
CCCCCCHHHHHHHCCCEEEEECCCHHCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCC
HLAFYIHNKVPILFIGDTLFSAGCGRIFEGTYKQMYNSLKKIKSLPKNTTIYCAHEYTKS
EEEEEEECCCEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCC
NLLWALDLEPENENIKNKLIEVEKKIALKELTIPFLLEEELIINLFLRADNLKRFSYLRA
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHEEEEEECCCCHHHHHEEC
NKDTWV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA