Click here to switch to the map view.

The map label for this gene is cobM [H]

Identifier: 123965758

GI number: 123965758

Start: 468588

End: 469343

Strand: Reverse

Name: cobM [H]

Synonym: P9515_05231

Alternate gene names: 123965758

Gene position: 469343-468588 (Counterclockwise)

Preceding gene: 123965759

Following gene: 123965756

Centisome position: 27.54

GC content: 32.54

Gene sequence:

>756_bases
ATGGATAAAAAAATCTCATTTATTGGAGTTGGTCCAGGCGATCCTGATTTATTAACAATTAAAGCGTTAAAAAAAATAAA
ATCAGCAGAGGTTATCTTTTGGGCTGATTCTTTAATTCCTGAAAAAATTATAAATTTTTCACTTGAGGGTTCTGAAAAAA
TAAAAACAAGTACGCTTACTTTAGAAAAAATAACGTCAATAATGATAAAAAGATACAATGAAGGTAAAACTATAATTAGA
TTGCATGATGGAGATCCATGCCTATATGGAGCAGTAAAAGAACAAATAGAAATACTAAAGCTAAACAACATCAAGACTGA
AGTCATCCCTGGTGTAAGTGCTTTTCAAGTTGCTGCTGCGTATCATCAGGCTGAGCTTACAATTCCGGATATTACTCAAA
CTATAATTCTGACGAGAGCAGGAGGCCGAACTGGTATGCCTGAGAAAGAATCTCTTAAGGAACTTGCGAAGCACAAATCC
TCTTTATGTCTTTATCTAAGTGCTAGGCACGTAAAGAATTCTCAAAAAATCTTATTAGAATTTTACCCTCCAGAAACTAA
AGTAATCGTTGGCTACAGAGTCTCTTGGGATGATGGTTGGACATCTCTAATTGAATTGAAGGATATGGAAAAATTTACTC
GTGAAAAAGAGATTATTAGAACTACTATTTATATTGTTAGTCCTGCAATTAAAAATATTACAAATAGGTCTAATCTTTAT
AACCCTTCTTACAATCATCTCTTTAGGAATAAATAA

Upstream 100 bases:

>100_bases
TTACGGATGGCTCAGTTTATTAGTATTGTTTTATTTTCCTCTGGATTAATTGGAATATTTTTTTTAAGATTAAAAACATA
TAATAAAAAAACAAGAAAGA

Downstream 100 bases:

>100_bases
TCGATTAAAGTTGATAGATAAATATAAATCACTATAATTGAGAAAAAATCAATTGCTTTGAACGAAATAAATTCTAATAA
TTCAGACAATAATTCAATAA

Product: putative precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MDKKISFIGVGPGDPDLLTIKALKKIKSAEVIFWADSLIPEKIINFSLEGSEKIKTSTLTLEKITSIMIKRYNEGKTIIR
LHDGDPCLYGAVKEQIEILKLNNIKTEVIPGVSAFQVAAAYHQAELTIPDITQTIILTRAGGRTGMPEKESLKELAKHKS
SLCLYLSARHVKNSQKILLEFYPPETKVIVGYRVSWDDGWTSLIELKDMEKFTREKEIIRTTIYIVSPAIKNITNRSNLY
NPSYNHLFRNK

Sequences:

>Translated_251_residues
MDKKISFIGVGPGDPDLLTIKALKKIKSAEVIFWADSLIPEKIINFSLEGSEKIKTSTLTLEKITSIMIKRYNEGKTIIR
LHDGDPCLYGAVKEQIEILKLNNIKTEVIPGVSAFQVAAAYHQAELTIPDITQTIILTRAGGRTGMPEKESLKELAKHKS
SLCLYLSARHVKNSQKILLEFYPPETKVIVGYRVSWDDGWTSLIELKDMEKFTREKEIIRTTIYIVSPAIKNITNRSNLY
NPSYNHLFRNK
>Mature_251_residues
MDKKISFIGVGPGDPDLLTIKALKKIKSAEVIFWADSLIPEKIINFSLEGSEKIKTSTLTLEKITSIMIKRYNEGKTIIR
LHDGDPCLYGAVKEQIEILKLNNIKTEVIPGVSAFQVAAAYHQAELTIPDITQTIILTRAGGRTGMPEKESLKELAKHKS
SLCLYLSARHVKNSQKILLEFYPPETKVIVGYRVSWDDGWTSLIELKDMEKFTREKEIIRTTIYIVSPAIKNITNRSNLY
NPSYNHLFRNK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=187, Percent_Identity=28.8770053475936, Blast_Score=92, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322922, Length=148, Percent_Identity=29.0540540540541, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28451; Mature: 28451

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKISFIGVGPGDPDLLTIKALKKIKSAEVIFWADSLIPEKIINFSLEGSEKIKTSTLT
CCCEEEEEEECCCCCCEEEHHHHHHHCCCEEEEEECCCCHHHHHCCCCCCCCCEEEHEEE
LEKITSIMIKRYNEGKTIIRLHDGDPCLYGAVKEQIEILKLNNIKTEVIPGVSAFQVAAA
HHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHEEEEEECCCCHHHCCCCHHHHHHHH
YHQAELTIPDITQTIILTRAGGRTGMPEKESLKELAKHKSSLCLYLSARHVKNSQKILLE
HHHCEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCCCEEEEE
FYPPETKVIVGYRVSWDDGWTSLIELKDMEKFTREKEIIRTTIYIVSPAIKNITNRSNLY
EECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHCCCCCCC
NPSYNHLFRNK
CCCHHHHCCCC
>Mature Secondary Structure
MDKKISFIGVGPGDPDLLTIKALKKIKSAEVIFWADSLIPEKIINFSLEGSEKIKTSTLT
CCCEEEEEEECCCCCCEEEHHHHHHHCCCEEEEEECCCCHHHHHCCCCCCCCCEEEHEEE
LEKITSIMIKRYNEGKTIIRLHDGDPCLYGAVKEQIEILKLNNIKTEVIPGVSAFQVAAA
HHHHHHHHHHHCCCCCEEEEEECCCCEEHHHHHHHEEEEEECCCCHHHCCCCHHHHHHHH
YHQAELTIPDITQTIILTRAGGRTGMPEKESLKELAKHKSSLCLYLSARHVKNSQKILLE
HHHCEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCCCEEEEE
FYPPETKVIVGYRVSWDDGWTSLIELKDMEKFTREKEIIRTTIYIVSPAIKNITNRSNLY
EECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHCCCCCCC
NPSYNHLFRNK
CCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]