Click here to switch to the map view.

The map label for this gene is ispD

Identifier: 123965752

GI number: 123965752

Start: 463893

End: 464564

Strand: Direct

Name: ispD

Synonym: P9515_05171

Alternate gene names: 123965752

Gene position: 463893-464564 (Clockwise)

Preceding gene: 123965750

Following gene: 123965755

Centisome position: 27.22

GC content: 31.4

Gene sequence:

>672_bases
GTGCATGTATTAATACCCGCTGCAGGTAGTGGCAGAAGAATGGAAGCTGGAAAAAATAAATTACTTATTGATTTAGAAGG
TGAATCTCTTATTTATTGGACTTTAAAATCTGTATTCTCTGCAAGTTTGGTAAGTTGGGTAGGAATAATTGGACAACCAA
ATGATAAAAAAAAATTATTAAATTCAGTAAAAAAATTTTCTAATGAAATTGAATGGATTAACGGTGGAAATACAAGGCAA
GAGTCTGTCTTTAATGGTTTAAATTCACTACCTTCTAATGCTGAGAAAGTTTTAATTCATGATGGTGCAAGATGCTTAAT
AAATCCTGACTTGATCAATAAATGTGCTTTGGAATTAGAAGAAAATGATGCAGTTATTTTAGCTACTAAAGTTACAGACA
CAATAAAAATAGTTGATAATGAAGGTTATATCCAACAAACGCCAAATAGACAAAATTTGTGGGCAGCTCAAACTCCTCAA
GGTTTTCTAGTGAAGAAATTAAGAGAAGCTCATGAAATGGCGATTGAGAAAAATTGGAAAGTTACAGATGATGCCTCACT
CTTTGAAATGTTGAATTGGCAAGTGAGAATTATTGAAGGGAATTCTTCGAACATAAAAATCACATCTCCATTAGATTTGC
AAATAGCGAAACTATTTTTAAAAGATTATTAG

Upstream 100 bases:

>100_bases
TTGACATTTGTAAATTTTTATAATTTAAGATCGTAGTACTTTTTGTGCAAACATATTGCTTTTATTTATAATTATCTAGA
AAATATAAATTCAAATTATT

Downstream 100 bases:

>100_bases
TAACAAGGAATAGAAATACTAAGTATTCCATTATTTCCATTTAATGTAGCTTTACAACCAAGAGGAATGCATGCATTCCC
AGATATATGTCCAATTGGGA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MHVLIPAAGSGRRMEAGKNKLLIDLEGESLIYWTLKSVFSASLVSWVGIIGQPNDKKKLLNSVKKFSNEIEWINGGNTRQ
ESVFNGLNSLPSNAEKVLIHDGARCLINPDLINKCALELEENDAVILATKVTDTIKIVDNEGYIQQTPNRQNLWAAQTPQ
GFLVKKLREAHEMAIEKNWKVTDDASLFEMLNWQVRIIEGNSSNIKITSPLDLQIAKLFLKDY

Sequences:

>Translated_223_residues
MHVLIPAAGSGRRMEAGKNKLLIDLEGESLIYWTLKSVFSASLVSWVGIIGQPNDKKKLLNSVKKFSNEIEWINGGNTRQ
ESVFNGLNSLPSNAEKVLIHDGARCLINPDLINKCALELEENDAVILATKVTDTIKIVDNEGYIQQTPNRQNLWAAQTPQ
GFLVKKLREAHEMAIEKNWKVTDDASLFEMLNWQVRIIEGNSSNIKITSPLDLQIAKLFLKDY
>Mature_223_residues
MHVLIPAAGSGRRMEAGKNKLLIDLEGESLIYWTLKSVFSASLVSWVGIIGQPNDKKKLLNSVKKFSNEIEWINGGNTRQ
ESVFNGLNSLPSNAEKVLIHDGARCLINPDLINKCALELEENDAVILATKVTDTIKIVDNEGYIQQTPNRQNLWAAQTPQ
GFLVKKLREAHEMAIEKNWKVTDDASLFEMLNWQVRIIEGNSSNIKITSPLDLQIAKLFLKDY

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=233, Percent_Identity=22.7467811158798, Blast_Score=67, Evalue=8e-12,
Organism=Escherichia coli, GI1789104, Length=220, Percent_Identity=31.8181818181818, Blast_Score=111, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_PROM5 (A2BVB5)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010833.1
- ProteinModelPortal:   A2BVB5
- SMR:   A2BVB5
- STRING:   A2BVB5
- GeneID:   4720030
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_05171
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- ProtClustDB:   PRK00155
- BioCyc:   PMAR167542:P9515ORF_0553-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25030; Mature: 25030

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVLIPAAGSGRRMEAGKNKLLIDLEGESLIYWTLKSVFSASLVSWVGIIGQPNDKKKLL
CEEEEECCCCCCEECCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHH
NSVKKFSNEIEWINGGNTRQESVFNGLNSLPSNAEKVLIHDGARCLINPDLINKCALELE
HHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEEECHHHHHHHHEEEC
ENDAVILATKVTDTIKIVDNEGYIQQTPNRQNLWAAQTPQGFLVKKLREAHEMAIEKNWK
CCCEEEEEEECCEEEEEEECCCCEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
VTDDASLFEMLNWQVRIIEGNSSNIKITSPLDLQIAKLFLKDY
CCCCHHHHHHCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MHVLIPAAGSGRRMEAGKNKLLIDLEGESLIYWTLKSVFSASLVSWVGIIGQPNDKKKLL
CEEEEECCCCCCEECCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHH
NSVKKFSNEIEWINGGNTRQESVFNGLNSLPSNAEKVLIHDGARCLINPDLINKCALELE
HHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEEECHHHHHHHHEEEC
ENDAVILATKVTDTIKIVDNEGYIQQTPNRQNLWAAQTPQGFLVKKLREAHEMAIEKNWK
CCCEEEEEEECCEEEEEEECCCCEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCC
VTDDASLFEMLNWQVRIIEGNSSNIKITSPLDLQIAKLFLKDY
CCCCHHHHHHCCCEEEEEECCCCCEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA