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The map label for this gene is hisF

Identifier: 123965727

GI number: 123965727

Start: 439900

End: 440673

Strand: Direct

Name: hisF

Synonym: P9515_04921

Alternate gene names: 123965727

Gene position: 439900-440673 (Clockwise)

Preceding gene: 123965723

Following gene: 123965728

Centisome position: 25.81

GC content: 34.88

Gene sequence:

>774_bases
ATGGTAGCTCTTCGTTTAATTCCTTGTTTAGATGTAGCTAATGGCAGGGTAGTTAAAGGTGTCAATTTTGTAAACCTTAG
AGACTCCGGCGATCCTGTTGAATTAGCTTGTAGATACTCAGAAGCAGGGGCGGATGAATTAGTCTTTTTAGATATTAGAG
CTAGTGTGGAAAATAGAAAAACATTAGTTGATCTTGTCTCTAGGACTGCGAAATCAGTAAAAATCCCTTTTACTGTTGGT
GGTGGAATAAATTCGATAGTCACAATAAATGATCTCTTAAGAGCAGGCGCAGATAAAGTAAGCTTGAATTCATCTGCAGT
TAAGAATCCAAAAATAATTTCTCAAAGCTCTAAAAAATTTGGAACACAGTGTATTGTGATAGCTATTGATGCAAGAAAAA
AACATAATGTATCTAATGAATGGGAAGTCTACGTTAAGGGGGGTAGAGAGAACACTGGTCTAGATGTGGTAAATTGGGCA
AAAAAAGTTGAGGAATTAGGAGCTGGAGAAATTTTATTAACTTCTATGGATGGAGATGGGACACAAAATGGATATGACTT
ATTATTAACTAAAACAGTTGCGAAGGCAGTTAATATTCCTGTTATAGCTTCAGGAGGTGCAGGCTCTTTAGAAGATATTT
TTGATGTTTTTACTGAAGGTGAAGCATCTGCAGCACTTTTAGCATCCTTACTTCATGATAAAAAACTTACCATTGAAGAA
ATAAAATCTTTTCTTATTAACAAGAAACTAATAATAAGGCCGAATGAAAAATAG

Upstream 100 bases:

>100_bases
ACTTTACTACCAATTTTAGGTAATAATTTTGTTTGAGCCATAAGAGTTAAAATGGAAGCTATTATGCAAGATTAACAGTC
TTTGGACAATTTTTTTTAAA

Downstream 100 bases:

>100_bases
CTTATTATTATTAATTTTTAAAATGATTAAATATGAGGCATAAAAAAATTAATGAGGTCAAAACTATTTTTAATAATATT
TCTTGTAGTTATGACTTTTT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MVALRLIPCLDVANGRVVKGVNFVNLRDSGDPVELACRYSEAGADELVFLDIRASVENRKTLVDLVSRTAKSVKIPFTVG
GGINSIVTINDLLRAGADKVSLNSSAVKNPKIISQSSKKFGTQCIVIAIDARKKHNVSNEWEVYVKGGRENTGLDVVNWA
KKVEELGAGEILLTSMDGDGTQNGYDLLLTKTVAKAVNIPVIASGGAGSLEDIFDVFTEGEASAALLASLLHDKKLTIEE
IKSFLINKKLIIRPNEK

Sequences:

>Translated_257_residues
MVALRLIPCLDVANGRVVKGVNFVNLRDSGDPVELACRYSEAGADELVFLDIRASVENRKTLVDLVSRTAKSVKIPFTVG
GGINSIVTINDLLRAGADKVSLNSSAVKNPKIISQSSKKFGTQCIVIAIDARKKHNVSNEWEVYVKGGRENTGLDVVNWA
KKVEELGAGEILLTSMDGDGTQNGYDLLLTKTVAKAVNIPVIASGGAGSLEDIFDVFTEGEASAALLASLLHDKKLTIEE
IKSFLINKKLIIRPNEK
>Mature_257_residues
MVALRLIPCLDVANGRVVKGVNFVNLRDSGDPVELACRYSEAGADELVFLDIRASVENRKTLVDLVSRTAKSVKIPFTVG
GGINSIVTINDLLRAGADKVSLNSSAVKNPKIISQSSKKFGTQCIVIAIDARKKHNVSNEWEVYVKGGRENTGLDVVNWA
KKVEELGAGEILLTSMDGDGTQNGYDLLLTKTVAKAVNIPVIASGGAGSLEDIFDVFTEGEASAALLASLLHDKKLTIEE
IKSFLINKKLIIRPNEK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=43.1906614785992, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=28.0334728033473, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=32.8025477707006, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_PROM5 (A2BV90)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010808.1
- ProteinModelPortal:   A2BV90
- SMR:   A2BV90
- STRING:   A2BV90
- GeneID:   4720005
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_04921
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- BioCyc:   PMAR167542:P9515ORF_0527-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27619; Mature: 27619

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALRLIPCLDVANGRVVKGVNFVNLRDSGDPVELACRYSEAGADELVFLDIRASVENRK
CCEEEEEEEEECCCCEEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEECCCCHHH
TLVDLVSRTAKSVKIPFTVGGGINSIVTINDLLRAGADKVSLNSSAVKNPKIISQSSKKF
HHHHHHHHHHHCEEEEEEECCCCCCEEEHHHHHHCCCCEEECCCCCCCCCHHCCCCCCCC
GTQCIVIAIDARKKHNVSNEWEVYVKGGRENTGLDVVNWAKKVEELGAGEILLTSMDGDG
CCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
TQNGYDLLLTKTVAKAVNIPVIASGGAGSLEDIFDVFTEGEASAALLASLLHDKKLTIEE
CCCCCEEEHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
IKSFLINKKLIIRPNEK
HHHHHCCCEEEEECCCC
>Mature Secondary Structure
MVALRLIPCLDVANGRVVKGVNFVNLRDSGDPVELACRYSEAGADELVFLDIRASVENRK
CCEEEEEEEEECCCCEEEECEEEEEECCCCCCEEEEEEECCCCCCEEEEEEEECCCCHHH
TLVDLVSRTAKSVKIPFTVGGGINSIVTINDLLRAGADKVSLNSSAVKNPKIISQSSKKF
HHHHHHHHHHHCEEEEEEECCCCCCEEEHHHHHHCCCCEEECCCCCCCCCHHCCCCCCCC
GTQCIVIAIDARKKHNVSNEWEVYVKGGRENTGLDVVNWAKKVEELGAGEILLTSMDGDG
CCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCC
TQNGYDLLLTKTVAKAVNIPVIASGGAGSLEDIFDVFTEGEASAALLASLLHDKKLTIEE
CCCCCEEEHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHH
IKSFLINKKLIIRPNEK
HHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA