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The map label for this gene is lepA [H]

Identifier: 123965716

GI number: 123965716

Start: 428930

End: 430738

Strand: Direct

Name: lepA [H]

Synonym: P9515_04811

Alternate gene names: 123965716

Gene position: 428930-430738 (Clockwise)

Preceding gene: 123965715

Following gene: 123965717

Centisome position: 25.17

GC content: 33.94

Gene sequence:

>1809_bases
ATGACTGATATATCCGTTTCAAAAATAAGAAATTTTTGCATAATTGCTCATATCGATCATGGTAAATCTACTCTCGCAGA
TAGATTACTTCAAGATACTGGAACTGTAAAACAAAGGGATATGCAAGATCAGTTTTTGGATAGTATGGATCTTGAAAGAG
AAAGAGGTATAACAATTAAATTGCAGGCAGCGAGGATGAAGTATAAAGCTGAAGATTCGCAAGAATATATCTTAAATCTA
ATTGATACCCCGGGGCATGTTGATTTTTCTTATGAAGTAAGTAGATCTTTACAGGCTTGTGAAGGTGCTTTATTAGTTGT
TGATGCAAGTCAAGGTGTAGAAGCACAAACTTTAGCTAATGTTTATCTTGCTTTAGAAAACAATTTAGAGATCATACCAG
TTTTAAATAAAGTTGATTTGCCTGGTGCAGATGCCAATAAAATAAAACAAGAAATTGAAGAGGTAATAGGATTAGATACT
TCTAATGCAATAAATTGCTCTGCAAAAACTGGAGAAGGGATAGAAGATATATTGGAAGCAGTTGTTAGTAGAATTCCTCA
TCCTCAAAATGAAATAAAATCTCCAACTAGGGCACTTATTTTTGACTCTTATTATGATCCTTATAGAGGAGTAATTGTTT
ACTTCAGAGTTATATCTGGGTCAATTAATAAAAGAGATAAGATCTTACTAATGGCGAGTAAGAAGCACTATGAATTAGAT
GAAATTGGAATAATGGCTCCTGATGAGAAGCAAGTTGATGAACTTCATGCTGGGGAAGTTGGTTATTTAGCGGCTTCTAT
TAAGTCTGTTGCTGATGCAAGAGTCGGAGATACAATTACACTATTTAATTCGGCAGCTAAAGAGCCCCTCCCTGGATATA
AAACAGCTAACCCAATGGTTTTTTGTGGATTGTTCCCAACAGATGCAGATCAATATCCAGATCTAAGAGAGTCTCTAGAA
AAGCTTCAATTATCTGATGCTGCACTTAAATATGAACCCGAGACTAGTAGCGCTATGGGCTTTGGATTTAGGTGTGGTTT
TTTAGGACTACTTCATATGGAAATTGTTCAGGAAAGGTTAGAGAGAGAATATGATTTGGATTTGATCGTAACTGCTCCTT
CAGTTATTTATAAAATTAATTTAAATGATAAGGAACAGATCTTTATCGATAATCCCTCGACAATCCCAGACCCACAATCA
AGAGAATCCATAGAAGAACCATATGTGAAAATGGAAATCTATTCTCCAAATGAATTTAATGGAACACTAATGGGTTTATG
CCAAGAAAGAAGAGGTATTTTTGTTGATATGAAATACATCACAACAGATAGGGTGACTTTGATTTACGAAATACCTTTAG
CTGAAGTTGTTACAGACTTTTTTGACCAAATGAAAAGTCGAACTCAAGGATATGCATCAATGGAATATCATTTGATTGGT
TATAGAAAAAATGATCTTGTTAGATTAGATGTTCTTATAAATTCCGAAAGAGCTGATCCTTTAACTTCAATTGTTCATAA
AGATAAGGCCTATGGTATTGGTAGAGGTTTAGTAGAAAAATTAAAGGAGCTTATACCTAAACAACAATTTAAAATCCCTA
TTCAAGCTTCAATAGGTAGTCGCATAATTGCGAGTGAAAGTATAAGTGCATTAAGAAAAGATGTTCTTTCGAAGTGCTAT
GGGGGAGATATTTCTAGAAAAAAGAAACTTTTAAAGAAGCAAGCTAAAGGTAAAAAGAGAATGAAAGCAATGGGTAAAGT
TGATGTACCTCAGGAAGCTTTTATGGCAGTTCTCAAATTAAATCAGTAA

Upstream 100 bases:

>100_bases
ATTTAAAAAAAAGAAATAATTCTATTTTAGGTTTCCATCCATAATCTTAATTAGCTAGTATTAATTAAGAAGATAAGAAA
GGAGTTGTTCTGAATTTTAT

Downstream 100 bases:

>100_bases
TAAATTTCAAATTAAAAACTAAAAGCTATTTTATCTTTCAAACAATAGTTATATTTTAGTTGATTCGAATAATCATAAAG
CTGTGAATGCAAATCCATCA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MTDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVKQRDMQDQFLDSMDLERERGITIKLQAARMKYKAEDSQEYILNL
IDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVLNKVDLPGADANKIKQEIEEVIGLDT
SNAINCSAKTGEGIEDILEAVVSRIPHPQNEIKSPTRALIFDSYYDPYRGVIVYFRVISGSINKRDKILLMASKKHYELD
EIGIMAPDEKQVDELHAGEVGYLAASIKSVADARVGDTITLFNSAAKEPLPGYKTANPMVFCGLFPTDADQYPDLRESLE
KLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKINLNDKEQIFIDNPSTIPDPQS
RESIEEPYVKMEIYSPNEFNGTLMGLCQERRGIFVDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIG
YRKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRGLVEKLKELIPKQQFKIPIQASIGSRIIASESISALRKDVLSKCY
GGDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKLNQ

Sequences:

>Translated_602_residues
MTDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVKQRDMQDQFLDSMDLERERGITIKLQAARMKYKAEDSQEYILNL
IDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVLNKVDLPGADANKIKQEIEEVIGLDT
SNAINCSAKTGEGIEDILEAVVSRIPHPQNEIKSPTRALIFDSYYDPYRGVIVYFRVISGSINKRDKILLMASKKHYELD
EIGIMAPDEKQVDELHAGEVGYLAASIKSVADARVGDTITLFNSAAKEPLPGYKTANPMVFCGLFPTDADQYPDLRESLE
KLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKINLNDKEQIFIDNPSTIPDPQS
RESIEEPYVKMEIYSPNEFNGTLMGLCQERRGIFVDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIG
YRKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRGLVEKLKELIPKQQFKIPIQASIGSRIIASESISALRKDVLSKCY
GGDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKLNQ
>Mature_601_residues
TDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVKQRDMQDQFLDSMDLERERGITIKLQAARMKYKAEDSQEYILNLI
DTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLANVYLALENNLEIIPVLNKVDLPGADANKIKQEIEEVIGLDTS
NAINCSAKTGEGIEDILEAVVSRIPHPQNEIKSPTRALIFDSYYDPYRGVIVYFRVISGSINKRDKILLMASKKHYELDE
IGIMAPDEKQVDELHAGEVGYLAASIKSVADARVGDTITLFNSAAKEPLPGYKTANPMVFCGLFPTDADQYPDLRESLEK
LQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIVTAPSVIYKINLNDKEQIFIDNPSTIPDPQSR
ESIEEPYVKMEIYSPNEFNGTLMGLCQERRGIFVDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIGY
RKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRGLVEKLKELIPKQQFKIPIQASIGSRIIASESISALRKDVLSKCYG
GDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKLNQ

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=48.5996705107084, Blast_Score=623, Evalue=1e-178,
Organism=Homo sapiens, GI94966754, Length=259, Percent_Identity=32.046332046332, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI25306283, Length=152, Percent_Identity=45.3947368421053, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=45.3947368421053, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=45.3947368421053, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI18390331, Length=155, Percent_Identity=40.6451612903226, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=39.3103448275862, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=43.3628318584071, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=43.3628318584071, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=43.3628318584071, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI217272894, Length=160, Percent_Identity=33.75, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI217272892, Length=160, Percent_Identity=33.75, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI94966752, Length=258, Percent_Identity=24.8062015503876, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI53729339, Length=219, Percent_Identity=27.8538812785388, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI53729337, Length=219, Percent_Identity=27.8538812785388, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI34147630, Length=255, Percent_Identity=27.0588235294118, Blast_Score=71, Evalue=4e-12,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=23.0769230769231, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=23.0769230769231, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=599, Percent_Identity=55.5926544240401, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=478, Percent_Identity=28.8702928870293, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=36.4779874213836, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790835, Length=155, Percent_Identity=35.4838709677419, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1789559, Length=227, Percent_Identity=26.431718061674, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=619, Percent_Identity=42.0032310177706, Blast_Score=506, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=36.7741935483871, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=144, Percent_Identity=39.5833333333333, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=211, Percent_Identity=29.8578199052133, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=211, Percent_Identity=29.8578199052133, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=36.1290322580645, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=146, Percent_Identity=34.9315068493151, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=249, Percent_Identity=27.3092369477912, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=42.8807947019868, Blast_Score=516, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6323098, Length=144, Percent_Identity=43.0555555555556, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=41.0958904109589, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=41.0958904109589, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=144, Percent_Identity=40.2777777777778, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324166, Length=143, Percent_Identity=41.2587412587413, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324761, Length=237, Percent_Identity=28.2700421940928, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322675, Length=154, Percent_Identity=29.2207792207792, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=44.7019867549669, Blast_Score=539, Evalue=1e-153,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=45.985401459854, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24582462, Length=149, Percent_Identity=40.2684563758389, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=38.7755102040816, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=38.7755102040816, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=38.7755102040816, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=33.9506172839506, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=38.4105960264901, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45550900, Length=280, Percent_Identity=27.1428571428571, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921738, Length=293, Percent_Identity=29.6928327645051, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363316, Length=234, Percent_Identity=29.4871794871795, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17864358, Length=234, Percent_Identity=29.4871794871795, Blast_Score=73, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28572034, Length=224, Percent_Identity=27.6785714285714, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67573; Mature: 67442

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVKQRDMQDQFLDSMDLERERGITIK
CCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCEEE
LQAARMKYKAEDSQEYILNLIDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLAN
EEHHHHHHCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHE
VYLALENNLEIIPVLNKVDLPGADANKIKQEIEEVIGLDTSNAINCSAKTGEGIEDILEA
EEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
VVSRIPHPQNEIKSPTRALIFDSYYDPYRGVIVYFRVISGSINKRDKILLMASKKHYELD
HHHHCCCCHHHHCCCCCEEEECCCCCCHHHHHHEEEECCCCCCCCCEEEEEECCCCCCHH
EIGIMAPDEKQVDELHAGEVGYLAASIKSVADARVGDTITLFNSAAKEPLPGYKTANPMV
HCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCCEE
FCGLFPTDADQYPDLRESLEKLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERL
EEEECCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLDLIVTAPSVIYKINLNDKEQIFIDNPSTIPDPQSRESIEEPYVKMEIYSPNEFN
HHHCCCEEEEECCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHCCEEEEEEECCCCCC
GTLMGLCQERRGIFVDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIG
CHHHHHHHHCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEE
YRKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRGLVEKLKELIPKQQFKIPIQASIGS
ECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCEEECCCCC
RIIASESISALRKDVLSKCYGGDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NQ
CC
>Mature Secondary Structure 
TDISVSKIRNFCIIAHIDHGKSTLADRLLQDTGTVKQRDMQDQFLDSMDLERERGITIK
CCCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHCCCEEE
LQAARMKYKAEDSQEYILNLIDTPGHVDFSYEVSRSLQACEGALLVVDASQGVEAQTLAN
EEHHHHHHCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHE
VYLALENNLEIIPVLNKVDLPGADANKIKQEIEEVIGLDTSNAINCSAKTGEGIEDILEA
EEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHH
VVSRIPHPQNEIKSPTRALIFDSYYDPYRGVIVYFRVISGSINKRDKILLMASKKHYELD
HHHHCCCCHHHHCCCCCEEEECCCCCCHHHHHHEEEECCCCCCCCCEEEEEECCCCCCHH
EIGIMAPDEKQVDELHAGEVGYLAASIKSVADARVGDTITLFNSAAKEPLPGYKTANPMV
HCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCCCEE
FCGLFPTDADQYPDLRESLEKLQLSDAALKYEPETSSAMGFGFRCGFLGLLHMEIVQERL
EEEECCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLDLIVTAPSVIYKINLNDKEQIFIDNPSTIPDPQSRESIEEPYVKMEIYSPNEFN
HHHCCCEEEEECCEEEEEEECCCCCEEEECCCCCCCCCCCHHHHHCCEEEEEEECCCCCC
GTLMGLCQERRGIFVDMKYITTDRVTLIYEIPLAEVVTDFFDQMKSRTQGYASMEYHLIG
CHHHHHHHHCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEEEE
YRKNDLVRLDVLINSERADPLTSIVHKDKAYGIGRGLVEKLKELIPKQQFKIPIQASIGS
ECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCEEECCCCC
RIIASESISALRKDVLSKCYGGDISRKKKLLKKQAKGKKRMKAMGKVDVPQEAFMAVLKL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA