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The map label for this gene is murE [H]

Identifier: 123965709

GI number: 123965709

Start: 421829

End: 423364

Strand: Direct

Name: murE [H]

Synonym: P9515_04741

Alternate gene names: 123965709

Gene position: 421829-423364 (Clockwise)

Preceding gene: 123965708

Following gene: 123965710

Centisome position: 24.75

GC content: 30.14

Gene sequence:

>1536_bases
ATGAGATCTGTAAAATTACATAAACTTTTAGATTTGGTAGGAATTATTCCTTCATTAGATTTAATCGATCATAAAATCAA
TAACATTTCTTTTAACTCTAAAGAAGTACAAAAAGGAACTTTATTTTTAGGTATGCCTGGTTTAAATGTTGATGGAGGAA
AATATTGTATTGAGGCAATTGAAAATGGCGCAGAGGCTGCCATTATTGGGTCTGCTGCAAAACAGAAAATTGGATCTATT
GATAGAAAAAGGATTTTGGTTATAGAGGATAATTTAGATTATATTTTTGGTCAAATAGTCGCTGAGTTTTGGAACAGGCC
TTCAAGAAAACTTAAACTTATTGGTGTTACGGGTACAAATGGAAAAACGACAATTACTTTTTTATTGGAATATCTTTTAA
AAAAATTAGGGAAAAAGACTGCATTATATGGGACCTTGTTCAATAGATGGCCTGGCTTCTCTGAAGTAGCCTCTCATACA
ACTGATTTTGCTGATAAACTTCAAAAGAAATTGAATGCTGCTGTTGAGGCAGAATCTGAATTCGCGATATTAGAGGTGAG
TTCTCATTCTATTGCTCAAAATAGGATATCAGGATGTGAATTTGAGGCGGCTATTTTTACTAATTTAACTCAAGATCATC
TTGATTATCACTCAGATATGGAATCTTATTTTCAAATAAAAAGAAAATTATTTTTCCCACCTTACTTAACAGAAAAGGAT
GGAATTTCTGTTTTAAATATTGATGACCCCTGGATATCTAAATTATCATTTGATCTTGAAAAAGGATCTTCATTAGTCTC
TACAAAGATTACTGATAGTGAATTTGAAAAAGATGATTTTTTTTTCGTAACAGATAAAAAATTTACTGAAAGTGGCTCCA
CCTGTATTTTTCATACACCTAGGGAAAAAATTAAACTGTTTGTTCCACTTGTTGGTGAATTTAATTTAATGAATGCGATT
CAAGCAATAACAATTTTGTATAAACTTAATTTCTCTTTAAAAGATTTATCAAAGTTAATACAATCTTTCCCCGGTGCTCC
AGGGAGGATGGAGAAAATAGAAATTGATAATGATGACGTTTCATCATTACTACCAACTTTAATTGTTGATTATGCGCATA
CACCTGATGGATTAAAGAAAGTTTTGAAATCAATTAAAAAACTTTGTGAGGGGAAACTTATAACTGTTTTTGGATGTGGC
GGAGATCGAGATTGTAGTAAAAGGCCTTTAATGGGATCAATAGCTGCAGAGTTTTCAGATCACTTGTTTATAACATCAGA
TAATCCAAGATCAGAAGATCCTCAAAAGATAGTAAATGATATTTTGATGGGTATAGAAAAAAGAGATCAAATAACAATCG
AAATTGATAGATTTAAAGCAATAAATGAATCTATTAAATTTGCCAATAAAGAAGATATTGTTTTAATTGCAGGAAAAGGT
CATGAAGATTATCAAATTCTCAATGATAAAGTTATTGATTTTGATGATAGGAAAATAGCTTATAAATTATTACAAGAAAA
AAGCAAATCTAAATAA

Upstream 100 bases:

>100_bases
TTATAGAATTGCCTCGTATTTCTCCAAGATTGAAGGATGATCAATTAAAGAATTGGTTTCAAAAAAATATTAATACCATT
CTGAATAAATAATTTTTTAT

Downstream 100 bases:

>100_bases
AATTTTCTAATCATATAAGAAATATCTTTACTCAAATCATAAGAAAAGTTTCTTAGAATCAATAAAGTTATTTTTTAATA
AAATGAAAGGGTTAGCCTTA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MRSVKLHKLLDLVGIIPSLDLIDHKINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAIENGAEAAIIGSAAKQKIGSI
DRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALYGTLFNRWPGFSEVASHT
TDFADKLQKKLNAAVEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQIKRKLFFPPYLTEKD
GISVLNIDDPWISKLSFDLEKGSSLVSTKITDSEFEKDDFFFVTDKKFTESGSTCIFHTPREKIKLFVPLVGEFNLMNAI
QAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIEIDNDDVSSLLPTLIVDYAHTPDGLKKVLKSIKKLCEGKLITVFGCG
GDRDCSKRPLMGSIAAEFSDHLFITSDNPRSEDPQKIVNDILMGIEKRDQITIEIDRFKAINESIKFANKEDIVLIAGKG
HEDYQILNDKVIDFDDRKIAYKLLQEKSKSK

Sequences:

>Translated_511_residues
MRSVKLHKLLDLVGIIPSLDLIDHKINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAIENGAEAAIIGSAAKQKIGSI
DRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALYGTLFNRWPGFSEVASHT
TDFADKLQKKLNAAVEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQIKRKLFFPPYLTEKD
GISVLNIDDPWISKLSFDLEKGSSLVSTKITDSEFEKDDFFFVTDKKFTESGSTCIFHTPREKIKLFVPLVGEFNLMNAI
QAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIEIDNDDVSSLLPTLIVDYAHTPDGLKKVLKSIKKLCEGKLITVFGCG
GDRDCSKRPLMGSIAAEFSDHLFITSDNPRSEDPQKIVNDILMGIEKRDQITIEIDRFKAINESIKFANKEDIVLIAGKG
HEDYQILNDKVIDFDDRKIAYKLLQEKSKSK
>Mature_511_residues
MRSVKLHKLLDLVGIIPSLDLIDHKINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAIENGAEAAIIGSAAKQKIGSI
DRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTNGKTTITFLLEYLLKKLGKKTALYGTLFNRWPGFSEVASHT
TDFADKLQKKLNAAVEAESEFAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQIKRKLFFPPYLTEKD
GISVLNIDDPWISKLSFDLEKGSSLVSTKITDSEFEKDDFFFVTDKKFTESGSTCIFHTPREKIKLFVPLVGEFNLMNAI
QAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIEIDNDDVSSLLPTLIVDYAHTPDGLKKVLKSIKKLCEGKLITVFGCG
GDRDCSKRPLMGSIAAEFSDHLFITSDNPRSEDPQKIVNDILMGIEKRDQITIEIDRFKAINESIKFANKEDIVLIAGKG
HEDYQILNDKVIDFDDRKIAYKLLQEKSKSK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=492, Percent_Identity=35.9756097560976, Blast_Score=268, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 57461; Mature: 57461

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVKLHKLLDLVGIIPSLDLIDHKINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAI
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
ENGAEAAIIGSAAKQKIGSIDRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTN
HCCCCEEEECHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
GKTTITFLLEYLLKKLGKKTALYGTLFNRWPGFSEVASHTTDFADKLQKKLNAAVEAESE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQIKRKLFFPPYLTEKD
EEEEEECCCHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GISVLNIDDPWISKLSFDLEKGSSLVSTKITDSEFEKDDFFFVTDKKFTESGSTCIFHTP
CEEEEECCCHHHHHHHHCHHCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEEECC
REKIKLFVPLVGEFNLMNAIQAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIEIDNDDV
HHHEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHH
SSLLPTLIVDYAHTPDGLKKVLKSIKKLCEGKLITVFGCGGDRDCSKRPLMGSIAAEFSD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
HLFITSDNPRSEDPQKIVNDILMGIEKRDQITIEIDRFKAINESIKFANKEDIVLIAGKG
CEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCEEEEECCC
HEDYQILNDKVIDFDDRKIAYKLLQEKSKSK
CCCHHHHCCCEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSVKLHKLLDLVGIIPSLDLIDHKINNISFNSKEVQKGTLFLGMPGLNVDGGKYCIEAI
CCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHH
ENGAEAAIIGSAAKQKIGSIDRKRILVIEDNLDYIFGQIVAEFWNRPSRKLKLIGVTGTN
HCCCCEEEECHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
GKTTITFLLEYLLKKLGKKTALYGTLFNRWPGFSEVASHTTDFADKLQKKLNAAVEAESE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FAILEVSSHSIAQNRISGCEFEAAIFTNLTQDHLDYHSDMESYFQIKRKLFFPPYLTEKD
EEEEEECCCHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GISVLNIDDPWISKLSFDLEKGSSLVSTKITDSEFEKDDFFFVTDKKFTESGSTCIFHTP
CEEEEECCCHHHHHHHHCHHCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEEECC
REKIKLFVPLVGEFNLMNAIQAITILYKLNFSLKDLSKLIQSFPGAPGRMEKIEIDNDDV
HHHEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCHH
SSLLPTLIVDYAHTPDGLKKVLKSIKKLCEGKLITVFGCGGDRDCSKRPLMGSIAAEFSD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHCCC
HLFITSDNPRSEDPQKIVNDILMGIEKRDQITIEIDRFKAINESIKFANKEDIVLIAGKG
CEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCEEEEECCC
HEDYQILNDKVIDFDDRKIAYKLLQEKSKSK
CCCHHHHCCCEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]