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The map label for this gene is lipB

Identifier: 123965698

GI number: 123965698

Start: 411050

End: 411694

Strand: Direct

Name: lipB

Synonym: P9515_04631

Alternate gene names: 123965698

Gene position: 411050-411694 (Clockwise)

Preceding gene: 123965693

Following gene: 123965699

Centisome position: 24.12

GC content: 27.75

Gene sequence:

>645_bases
ATGATTAATAGAACATCAATAATTAAACAACCTGATAAAATTTCATCTTTTTGTGATGTATATAAATTACAAAAAAAATA
TCAGGACGCATTAATTTCAGGTAAATCAAATATCGATTTTATTTGGTTAGGAGAACATCAACTTTGTTATACCATTGGAA
GAGGATCGAATATGGGTAACTTACTTTTCTCATTAGACGAGCAAGATGTATTTAAAATTGATAGAGGAGGGGAGGTTACT
TGTCATATGCCAGGACAGTTAGTCACCTATTTGGTTTTAGATCTGCATAATTTTAATAAAGATTTAAATTGGTATTTAAG
AAAAATTGAAAAAATAATAATTAAAGTTCTCAGTAGTTTTAATATTGATTCCTCTACTAAAGATGGATTTACGGGGGTAT
GGACTGGAGAAAGAAAAATTGCTTCTATTGGAATTGGATGCAAAAGATGGGTCACAATTCATGGCTTTTCAATTAATGTA
AACTGCAAATTAGAAAATTTTGATAAAATCGTACCTTGTGGTATCCAGGGTTGCCAAATGGCAAATATGTCTGATTACAA
AAAAAATTTAGATATTAAAGAAGTTAAAATAATTGTTAAAAAAATCATTCAGGAAGAATTTTATTTTAATTTTATATCAG
AATAG

Upstream 100 bases:

>100_bases
TATTCTTTCAATGCACCTGTAAGTTCAATATTCTTCCCGTGGATTAAAATTTTCATAATAAATCTAAAAATATGTTCATA
CTTACTAAATCTACTTACAT

Downstream 100 bases:

>100_bases
ATACTAAAAATAGTTTGGAAAGATTATAAATATGAATGAACTAGCATATTGGCCCGCATCAAAATCTCATCCAAAAAATG
AGAAATTTATTAGAGAAAGA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MINRTSIIKQPDKISSFCDVYKLQKKYQDALISGKSNIDFIWLGEHQLCYTIGRGSNMGNLLFSLDEQDVFKIDRGGEVT
CHMPGQLVTYLVLDLHNFNKDLNWYLRKIEKIIIKVLSSFNIDSSTKDGFTGVWTGERKIASIGIGCKRWVTIHGFSINV
NCKLENFDKIVPCGIQGCQMANMSDYKKNLDIKEVKIIVKKIIQEEFYFNFISE

Sequences:

>Translated_214_residues
MINRTSIIKQPDKISSFCDVYKLQKKYQDALISGKSNIDFIWLGEHQLCYTIGRGSNMGNLLFSLDEQDVFKIDRGGEVT
CHMPGQLVTYLVLDLHNFNKDLNWYLRKIEKIIIKVLSSFNIDSSTKDGFTGVWTGERKIASIGIGCKRWVTIHGFSINV
NCKLENFDKIVPCGIQGCQMANMSDYKKNLDIKEVKIIVKKIIQEEFYFNFISE
>Mature_214_residues
MINRTSIIKQPDKISSFCDVYKLQKKYQDALISGKSNIDFIWLGEHQLCYTIGRGSNMGNLLFSLDEQDVFKIDRGGEVT
CHMPGQLVTYLVLDLHNFNKDLNWYLRKIEKIIIKVLSSFNIDSSTKDGFTGVWTGERKIASIGIGCKRWVTIHGFSINV
NCKLENFDKIVPCGIQGCQMANMSDYKKNLDIKEVKIIVKKIIQEEFYFNFISE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=149, Percent_Identity=31.5436241610738, Blast_Score=78, Evalue=4e-15,
Organism=Escherichia coli, GI87081767, Length=153, Percent_Identity=35.2941176470588, Blast_Score=108, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323268, Length=119, Percent_Identity=37.8151260504202, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355483, Length=165, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_PROM5 (A2BV61)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010779.1
- ProteinModelPortal:   A2BV61
- SMR:   A2BV61
- STRING:   A2BV61
- GeneID:   4719287
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_04631
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   GDEIWVV
- ProtClustDB:   PRK14344
- BioCyc:   PMAR167542:P9515ORF_0496-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24596; Mature: 24596

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRTSIIKQPDKISSFCDVYKLQKKYQDALISGKSNIDFIWLGEHQLCYTIGRGSNMGN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCC
LLFSLDEQDVFKIDRGGEVTCHMPGQLVTYLVLDLHNFNKDLNWYLRKIEKIIIKVLSSF
EEEEECCCCEEEECCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
NIDSSTKDGFTGVWTGERKIASIGIGCKRWVTIHGFSINVNCKLENFDKIVPCGIQGCQM
CCCCCCCCCCEEEEECCCEEEEECCCCEEEEEEEEEEEEEEEEECCCCCCCCCCCCCCEE
ANMSDYKKNLDIKEVKIIVKKIIQEEFYFNFISE
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINRTSIIKQPDKISSFCDVYKLQKKYQDALISGKSNIDFIWLGEHQLCYTIGRGSNMGN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCCC
LLFSLDEQDVFKIDRGGEVTCHMPGQLVTYLVLDLHNFNKDLNWYLRKIEKIIIKVLSSF
EEEEECCCCEEEECCCCEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
NIDSSTKDGFTGVWTGERKIASIGIGCKRWVTIHGFSINVNCKLENFDKIVPCGIQGCQM
CCCCCCCCCCEEEEECCCEEEEECCCCEEEEEEEEEEEEEEEEECCCCCCCCCCCCCCEE
ANMSDYKKNLDIKEVKIIVKKIIQEEFYFNFISE
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA