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The map label for this gene is cca [H]

Identifier: 123965571

GI number: 123965571

Start: 308980

End: 310227

Strand: Reverse

Name: cca [H]

Synonym: P9515_03361

Alternate gene names: 123965571

Gene position: 310227-308980 (Counterclockwise)

Preceding gene: 123965572

Following gene: 123965569

Centisome position: 18.2

GC content: 26.44

Gene sequence:

>1248_bases
ATGAATGATATTTCGGCTCATATAAATCAATCAATATTAAAAATTCCTTTTGACTTATTTAATATAATTTCTAATTATAT
TAAATTGCATAGCAACGTTAAAGTTGCAATAGTAGGTGGCTATATACGAGATTTATTAATCAATAAAATTCATAAAAAGA
CTTTTTTCAGCCCAATCGACATAGATATAGTTACTGAAGGATCTTCAGTTGATCTAGCAAAATTCATAAAAAAAAACATA
TCAAATGTAGAACTTTGCCTAATAAAAGAGTTTGAAATATATGACACTGTTGAATTAAATATTAACAATTTAAAAATTGA
TATAGCTTCTGCAAGAAAAGAAACCTATGAAGCTCCAGGATTAAATCCAATCGTACAGCATACAAGTATTGAAGAGGATT
TAAAAAGAAGAGATTTTAGTATAAATGCCATAGCTTTTGAATTCTCAAAGCAAGAAATTTATGATTACTTTGATGGCATC
AAACATATAAAAGAAAAAGAATTACATTTGCTGCATGAAAATAGTATTCAAGATGATCCTAGTAGAATAATAAGATGCGC
AAGATATGCATCGAGATTAGGTTTTAAAATTTCAAAAAAATCACTTAAACAATCTCAAGATACTGTCAAGAGATGGCCAT
GGGCAGTTATTAACGATGATACTAAATCTAGATTTCCTCCAGGAATAAGTATTAGAATCAGGATGGAATTGTCAGAAATA
TACAAATACGACAATCTTGCAAAAATAGTTTCTTTACTAAATGATTGGGAAGTTATTTCTATTTTAAATAAAAATATTAG
TATTGATAATAAATTTTTAAGGGGTTTAAAGTGGATTAAAAAATTAAAGGGAAATTATATTCTATACTTATTAAAAAATT
CAGAAAATTTTGAGCTAACATGTCAAAGATTTTTTATTAATAGCAAGGAAAAGAAAATACTCAAAGATTACCTAAATATT
TCAAATCAATTAGAAAATGAAAAAGAAAAATTTCTCTATTTGACTCCTTCAAATTGGACTGAATTTATTGAAACAAAAAA
CCTAGATGAAGAAACAGTTAAATTGTTAATTTGCAACGGGGGGGAATTTTGGAAATCATTTTTTAGATGGCTTTTTATTT
ATAAATTTATTAAATCAAAGAAAAGTGGAGCATCATTAAAAAGTGAAGGATGGCATCCGGGGCAAGAAATGGGTAATGAA
ATTAAAAGGTTGAGATATATAGAGATCGATAAAATTAAAAGAAACTGA

Upstream 100 bases:

>100_bases
AGTGGAGAAAAAATATCGTTAGCAGTTAAATTTGGAGATAAAGGTAGCAAAATTTTAGACCCAAGATTAGCTCCAATAAG
AGCAATAAACTGAAATTGAA

Downstream 100 bases:

>100_bases
CTATCAATTTATTACTTCTCCAACCTTATACCATTCATCGTTTAAAAAACTTTCATACTTAGGATCACAACTGATCAAAA
GTGGGCCACATGTTTGCGGG

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNDISAHINQSILKIPFDLFNIISNYIKLHSNVKVAIVGGYIRDLLINKIHKKTFFSPIDIDIVTEGSSVDLAKFIKKNI
SNVELCLIKEFEIYDTVELNINNLKIDIASARKETYEAPGLNPIVQHTSIEEDLKRRDFSINAIAFEFSKQEIYDYFDGI
KHIKEKELHLLHENSIQDDPSRIIRCARYASRLGFKISKKSLKQSQDTVKRWPWAVINDDTKSRFPPGISIRIRMELSEI
YKYDNLAKIVSLLNDWEVISILNKNISIDNKFLRGLKWIKKLKGNYILYLLKNSENFELTCQRFFINSKEKKILKDYLNI
SNQLENEKEKFLYLTPSNWTEFIETKNLDEETVKLLICNGGEFWKSFFRWLFIYKFIKSKKSGASLKSEGWHPGQEMGNE
IKRLRYIEIDKIKRN

Sequences:

>Translated_415_residues
MNDISAHINQSILKIPFDLFNIISNYIKLHSNVKVAIVGGYIRDLLINKIHKKTFFSPIDIDIVTEGSSVDLAKFIKKNI
SNVELCLIKEFEIYDTVELNINNLKIDIASARKETYEAPGLNPIVQHTSIEEDLKRRDFSINAIAFEFSKQEIYDYFDGI
KHIKEKELHLLHENSIQDDPSRIIRCARYASRLGFKISKKSLKQSQDTVKRWPWAVINDDTKSRFPPGISIRIRMELSEI
YKYDNLAKIVSLLNDWEVISILNKNISIDNKFLRGLKWIKKLKGNYILYLLKNSENFELTCQRFFINSKEKKILKDYLNI
SNQLENEKEKFLYLTPSNWTEFIETKNLDEETVKLLICNGGEFWKSFFRWLFIYKFIKSKKSGASLKSEGWHPGQEMGNE
IKRLRYIEIDKIKRN
>Mature_415_residues
MNDISAHINQSILKIPFDLFNIISNYIKLHSNVKVAIVGGYIRDLLINKIHKKTFFSPIDIDIVTEGSSVDLAKFIKKNI
SNVELCLIKEFEIYDTVELNINNLKIDIASARKETYEAPGLNPIVQHTSIEEDLKRRDFSINAIAFEFSKQEIYDYFDGI
KHIKEKELHLLHENSIQDDPSRIIRCARYASRLGFKISKKSLKQSQDTVKRWPWAVINDDTKSRFPPGISIRIRMELSEI
YKYDNLAKIVSLLNDWEVISILNKNISIDNKFLRGLKWIKKLKGNYILYLLKNSENFELTCQRFFINSKEKKILKDYLNI
SNQLENEKEKFLYLTPSNWTEFIETKNLDEETVKLLICNGGEFWKSFFRWLFIYKFIKSKKSGASLKSEGWHPGQEMGNE
IKRLRYIEIDKIKRN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48796; Mature: 48796

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDISAHINQSILKIPFDLFNIISNYIKLHSNVKVAIVGGYIRDLLINKIHKKTFFSPID
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCC
IDIVTEGSSVDLAKFIKKNISNVELCLIKEFEIYDTVELNINNLKIDIASARKETYEAPG
EEEEECCCCCHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEECEEEEEEEHHHHHHCCCCC
LNPIVQHTSIEEDLKRRDFSINAIAFEFSKQEIYDYFDGIKHIKEKELHLLHENSIQDDP
CCHHHHHCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SRIIRCARYASRLGFKISKKSLKQSQDTVKRWPWAVINDDTKSRFPPGISIRIRMELSEI
HHHHHHHHHHHHHCCEECHHHHHHHHHHHHHCCCEEECCCCHHCCCCCCEEEEEEEHHHH
YKYDNLAKIVSLLNDWEVISILNKNISIDNKFLRGLKWIKKLKGNYILYLLKNSENFELT
HHHCCHHHHHHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEE
CQRFFINSKEKKILKDYLNISNQLENEKEKFLYLTPSNWTEFIETKNLDEETVKLLICNG
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCHHEEEEEECC
GEFWKSFFRWLFIYKFIKSKKSGASLKSEGWHPGQEMGNEIKRLRYIEIDKIKRN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEHHCCCCC
>Mature Secondary Structure
MNDISAHINQSILKIPFDLFNIISNYIKLHSNVKVAIVGGYIRDLLINKIHKKTFFSPID
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCCC
IDIVTEGSSVDLAKFIKKNISNVELCLIKEFEIYDTVELNINNLKIDIASARKETYEAPG
EEEEECCCCCHHHHHHHCCCCCEEEEEEEEEEEEEEEEEEECEEEEEEEHHHHHHCCCCC
LNPIVQHTSIEEDLKRRDFSINAIAFEFSKQEIYDYFDGIKHIKEKELHLLHENSIQDDP
CCHHHHHCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
SRIIRCARYASRLGFKISKKSLKQSQDTVKRWPWAVINDDTKSRFPPGISIRIRMELSEI
HHHHHHHHHHHHHCCEECHHHHHHHHHHHHHCCCEEECCCCHHCCCCCCEEEEEEEHHHH
YKYDNLAKIVSLLNDWEVISILNKNISIDNKFLRGLKWIKKLKGNYILYLLKNSENFELT
HHHCCHHHHHHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEE
CQRFFINSKEKKILKDYLNISNQLENEKEKFLYLTPSNWTEFIETKNLDEETVKLLICNG
EEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHCCCCCHHEEEEEECC
GEFWKSFFRWLFIYKFIKSKKSGASLKSEGWHPGQEMGNEIKRLRYIEIDKIKRN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]