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The map label for this gene is clpB2 [H]

Identifier: 123965471

GI number: 123965471

Start: 215837

End: 218584

Strand: Reverse

Name: clpB2 [H]

Synonym: P9515_02361

Alternate gene names: 123965471

Gene position: 218584-215837 (Counterclockwise)

Preceding gene: 123965473

Following gene: 123965470

Centisome position: 12.83

GC content: 30.39

Gene sequence:

>2748_bases
ATGAGAGAAACTCTTACATCAAATCCAGAACTTTTTAGTGATATAAGTTGGAATCTTCTTTTGTTAGGAGAAGAAACTGC
AAAGAAATGGGACCATAGTGAATTCAATATTGAACACATTATTCATACTCTGTTTACTTCAAGTGATTTTTTTGCATTCA
TAGAAAAATTATCAATAGATCAAGATACAGTTTTAGACATAACTGAGGATTTTCTAGAGGAGACACCCACAAATGATTCA
GATATTTTTACTATTGGTGAAGATTTAGAGATTTTACTAGATAATGCAAATCAAATAAAAACTCAGTGGGGATCTAATTT
AATAGAGATTCCTCATTTGTTAATTGCATTAGGTAGAGATGCAAGAATAGGAAATTATGTTTTTCAAGAAGGGGACCTTT
CAGTAGAACAATTAGAGGAAGAATTAAAAATTTTTCCCAATATTAATCCAAGGCAAAATTACCTTGAGAATAAAAAAAAT
ATTACCAATAAAGAGAATAAATCAATAAATAAAACTGATAAAAAAGCCCTAATAGAAAAAACAAAACCTATAATTACCAC
ACCGAAAAATAATCTGAAAATTGAAACTCAACATAATAGTAGCCAGAATGCACTCTCTCTTTATGGAAAAGATTTAACTG
ACTCAGCGAAAAAAGGTTTGCTCGATCCAGTTGTCGGACGAGAGAATGAAATTAATAATTTAATGAGAGTTTTATCTAGG
CGTAGTAAAAATAACCCTATCCTCATTGGTTATCCTGGAGTTGGCAAAACTTCAATTGCTAAATTACTTGCTCAACTGAT
AGTGGAAAGAAAAGTTCCTGATTCATTAAAAAACTTAAAAATTATTTCTCTTGACTTAGGAGCATTAGTTTCCGGAACAA
AATTTAGAGGTCAATTAGAAGAAAGACTAAGTTTAATACTTAACGAAATAAAAGACTCAAATCAGGGCATAATTTTATTT
ATTGATGAAATTCATACAATAATTAGTTCTGATAGATCTACAGCAGATATAAGCAATATCTTAAAACCTTTACTTACTGA
GGGGGAACTGAGATGTATAGGGACAACCACTCCTGAAAAGTTTAGAGAAAATATTGAAAGAGATCAAGCATTAAATAACT
GTTTTCAAAAAATATTAGTTAATGAGCCTTCAGTAGAATTAACTGCGCAAATTTTGAAAGGTATAAAAAAGAAATATGAA
TTACACCATGGTATTAGAATTACCGAAGAAGCGATAAATTCTTCTGCAAGACTTGCAGATCGATATATAAGCGATAAGTT
TCTTCCGGATAAAGCAATAGATTTGATTGACGAAGCAGCAGCTCAATTAAAGATAGAATTTAATATAAAACCTCAAATAA
TCCTAGATGAAGAAAGCAAAATTTATAACATTGAAAATAAGTTAAAAGATCTTTCACCTGAAAACATTGAAGATAGAGAA
AAATTATTAGAGAGAAAAGAATTAGCTACAGATAAATTGAACGCAATTATCAATAATTGGAATAATGATCAGGAAAAAAT
GGAACAATTAACTTTTTTGTTACATGAAGAGGACAGACTTATTCTCGAAATCGAAGAATTAAATATGAACAGCGAGGAAA
TAAATTATTTTGAGCGTTTGAATAACCTAGAGGAAGAACTTAATGAGGTCGAATTTGAAATAAACAACATTGAGAGCAGA
TTCAAAAATCCTCAAAGGGATAAGAAATTTAATTTTAAATATCAAGTTGAACCTGATGATATCGCTGATGTTATATCAAA
AATAACTGGTATTCCTATGGCTAAAGTAGTTTCTAATGAACGAAAAAAATTAGTTAATTTAGAGTTAGAAATAAGCGAAA
AAGTTATTGGGCAGAAAAAAGCTATAGAAGCTGTTTCTTCTGCAATAAGAAGAGCGAGAGTTGGAATGAAAAATCCTAAA
AGACCTGTTGGATCATTCTTATTTATGGGTCCTACAGGAGTTGGGAAAACGGAATTAGCAAAATCTCTAGCATCATCATT
ATTTGATGAAGAAGAAGCCTTATTAAGGTTGGATATGAGTGAATATATGGAAAAAAATGCTGTCGCTAGGCTTTTAGGAG
CACCTCCAGGTTATGTCGGTTACGAAGAGGGGGGGCAGTTAACTGAAGCAGTCAGGAGAAAACCTTATTCCGTAATTCTT
CTTGATGAGATCGAAAAAGCTCATTCAGAAGTTTTCAACGTCCTCCTACAGGTTTTAGATGAAGGAAGATTAACTGATTC
TCAAGGAAGAACAGTTGACTTCAAAAATACAGTAATCATAATGACAAGTAATTTAGCTGGAAAAATAATCTTAGAAGATG
CCAAAAAAATTCCAAACAATAAAGAAAACTCTGAATTAAGAGAACATGCTTTACAAGAGTCTATTAATAAGTCATTATCA
TCTATTTTTAGACCAGAATTTCTAAACAGAATCGATGAGGTAGTAAAGTTTGATCTCCTCTCAATTGATCAACTACAAAA
AATAATTCTTTTACAAATAAACGAATTAAAGAAATTACTTCTTGAGCAGGGAATAAACATTAATATTGATAAAAAAGTTA
TCTTAAAAATTGCGAATGATTCTTATCAACCTGAATATGGTGCTAGGCCCTTAAGTAGAGAGCTTAGAAGACAAATAGAA
AACCCACTAGCATCAAGATTACTAGAGGAAAATTTTAAAAATAAAAAAAATATAAGTGTAAAGATTAATCCTTCAAAAAA
AGATGAGATTTTATTTAAACCTAGCTGA

Upstream 100 bases:

>100_bases
ATTTTAATTTCATCTTATAATTAAAAAACTCTAATGATCATTAAGGGTTATCTTAATTAAGATAAAAATTGAATGGTCAA
GTAATACTTTTGTATAGTTC

Downstream 100 bases:

>100_bases
TGAGTAAATCAATGAGCTAAAATAGTTTTAAGCTTTCGAAGCCTATACTAAAATAAAATTTTGTGTCAAGTCCTACTAAT
CAAAAACCTCTTAAAAAAGA

Product: putative ATP-dependent Clp protease, Hsp 100, ATP-binding subunit ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 915; Mature: 915

Protein sequence:

>915_residues
MRETLTSNPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFTSSDFFAFIEKLSIDQDTVLDITEDFLEETPTNDS
DIFTIGEDLEILLDNANQIKTQWGSNLIEIPHLLIALGRDARIGNYVFQEGDLSVEQLEEELKIFPNINPRQNYLENKKN
ITNKENKSINKTDKKALIEKTKPIITTPKNNLKIETQHNSSQNALSLYGKDLTDSAKKGLLDPVVGRENEINNLMRVLSR
RSKNNPILIGYPGVGKTSIAKLLAQLIVERKVPDSLKNLKIISLDLGALVSGTKFRGQLEERLSLILNEIKDSNQGIILF
IDEIHTIISSDRSTADISNILKPLLTEGELRCIGTTTPEKFRENIERDQALNNCFQKILVNEPSVELTAQILKGIKKKYE
LHHGIRITEEAINSSARLADRYISDKFLPDKAIDLIDEAAAQLKIEFNIKPQIILDEESKIYNIENKLKDLSPENIEDRE
KLLERKELATDKLNAIINNWNNDQEKMEQLTFLLHEEDRLILEIEELNMNSEEINYFERLNNLEEELNEVEFEINNIESR
FKNPQRDKKFNFKYQVEPDDIADVISKITGIPMAKVVSNERKKLVNLELEISEKVIGQKKAIEAVSSAIRRARVGMKNPK
RPVGSFLFMGPTGVGKTELAKSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYSVIL
LDEIEKAHSEVFNVLLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKIILEDAKKIPNNKENSELREHALQESINKSLS
SIFRPEFLNRIDEVVKFDLLSIDQLQKIILLQINELKKLLLEQGININIDKKVILKIANDSYQPEYGARPLSRELRRQIE
NPLASRLLEENFKNKKNISVKINPSKKDEILFKPS

Sequences:

>Translated_915_residues
MRETLTSNPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFTSSDFFAFIEKLSIDQDTVLDITEDFLEETPTNDS
DIFTIGEDLEILLDNANQIKTQWGSNLIEIPHLLIALGRDARIGNYVFQEGDLSVEQLEEELKIFPNINPRQNYLENKKN
ITNKENKSINKTDKKALIEKTKPIITTPKNNLKIETQHNSSQNALSLYGKDLTDSAKKGLLDPVVGRENEINNLMRVLSR
RSKNNPILIGYPGVGKTSIAKLLAQLIVERKVPDSLKNLKIISLDLGALVSGTKFRGQLEERLSLILNEIKDSNQGIILF
IDEIHTIISSDRSTADISNILKPLLTEGELRCIGTTTPEKFRENIERDQALNNCFQKILVNEPSVELTAQILKGIKKKYE
LHHGIRITEEAINSSARLADRYISDKFLPDKAIDLIDEAAAQLKIEFNIKPQIILDEESKIYNIENKLKDLSPENIEDRE
KLLERKELATDKLNAIINNWNNDQEKMEQLTFLLHEEDRLILEIEELNMNSEEINYFERLNNLEEELNEVEFEINNIESR
FKNPQRDKKFNFKYQVEPDDIADVISKITGIPMAKVVSNERKKLVNLELEISEKVIGQKKAIEAVSSAIRRARVGMKNPK
RPVGSFLFMGPTGVGKTELAKSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYSVIL
LDEIEKAHSEVFNVLLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKIILEDAKKIPNNKENSELREHALQESINKSLS
SIFRPEFLNRIDEVVKFDLLSIDQLQKIILLQINELKKLLLEQGININIDKKVILKIANDSYQPEYGARPLSRELRRQIE
NPLASRLLEENFKNKKNISVKINPSKKDEILFKPS
>Mature_915_residues
MRETLTSNPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFTSSDFFAFIEKLSIDQDTVLDITEDFLEETPTNDS
DIFTIGEDLEILLDNANQIKTQWGSNLIEIPHLLIALGRDARIGNYVFQEGDLSVEQLEEELKIFPNINPRQNYLENKKN
ITNKENKSINKTDKKALIEKTKPIITTPKNNLKIETQHNSSQNALSLYGKDLTDSAKKGLLDPVVGRENEINNLMRVLSR
RSKNNPILIGYPGVGKTSIAKLLAQLIVERKVPDSLKNLKIISLDLGALVSGTKFRGQLEERLSLILNEIKDSNQGIILF
IDEIHTIISSDRSTADISNILKPLLTEGELRCIGTTTPEKFRENIERDQALNNCFQKILVNEPSVELTAQILKGIKKKYE
LHHGIRITEEAINSSARLADRYISDKFLPDKAIDLIDEAAAQLKIEFNIKPQIILDEESKIYNIENKLKDLSPENIEDRE
KLLERKELATDKLNAIINNWNNDQEKMEQLTFLLHEEDRLILEIEELNMNSEEINYFERLNNLEEELNEVEFEINNIESR
FKNPQRDKKFNFKYQVEPDDIADVISKITGIPMAKVVSNERKKLVNLELEISEKVIGQKKAIEAVSSAIRRARVGMKNPK
RPVGSFLFMGPTGVGKTELAKSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYSVIL
LDEIEKAHSEVFNVLLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKIILEDAKKIPNNKENSELREHALQESINKSLS
SIFRPEFLNRIDEVVKFDLLSIDQLQKIILLQINELKKLLLEQGININIDKKVILKIANDSYQPEYGARPLSRELRRQIE
NPLASRLLEENFKNKKNISVKINPSKKDEILFKPS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=324, Percent_Identity=35.8024691358025, Blast_Score=197, Evalue=4e-50,
Organism=Escherichia coli, GI1788943, Length=712, Percent_Identity=45.6460674157303, Blast_Score=620, Evalue=1e-178,
Organism=Escherichia coli, GI1787109, Length=336, Percent_Identity=42.8571428571429, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6320464, Length=726, Percent_Identity=42.6997245179063, Blast_Score=569, Evalue=1e-163,
Organism=Saccharomyces cerevisiae, GI6323002, Length=926, Percent_Identity=34.341252699784, Blast_Score=508, Evalue=1e-144,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 104473; Mature: 104473

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRETLTSNPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFTSSDFFAFIEKLSID
CCCCCCCCCHHHHCCCCEEEEEECHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC
QDTVLDITEDFLEETPTNDSDIFTIGEDLEILLDNANQIKTQWGSNLIEIPHLLIALGRD
CHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCC
ARIGNYVFQEGDLSVEQLEEELKIFPNINPRQNYLENKKNITNKENKSINKTDKKALIEK
CCCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
TKPIITTPKNNLKIETQHNSSQNALSLYGKDLTDSAKKGLLDPVVGRENEINNLMRVLSR
CCCEEECCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RSKNNPILIGYPGVGKTSIAKLLAQLIVERKVPDSLKNLKIISLDLGALVSGTKFRGQLE
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCEEEEEEHHHHHCCCHHHHHHH
ERLSLILNEIKDSNQGIILFIDEIHTIISSDRSTADISNILKPLLTEGELRCIGTTTPEK
HHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCHHH
FRENIERDQALNNCFQKILVNEPSVELTAQILKGIKKKYELHHGIRITEEAINSSARLAD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHH
RYISDKFLPDKAIDLIDEAAAQLKIEFNIKPQIILDEESKIYNIENKLKDLSPENIEDRE
HHHHHCCCCHHHHHHHHHHHHEEEEEEECCCEEEECCCCCEEEHHHHHHCCCCCCCHHHH
KLLERKELATDKLNAIINNWNNDQEKMEQLTFLLHEEDRLILEIEELNMNSEEINYFERL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
NNLEEELNEVEFEINNIESRFKNPQRDKKFNFKYQVEPDDIADVISKITGIPMAKVVSNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHH
RKKLVNLELEISEKVIGQKKAIEAVSSAIRRARVGMKNPKRPVGSFLFMGPTGVGKTELA
HHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHH
KSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYSVIL
HHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEE
LDEIEKAHSEVFNVLLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKIILEDAKKIPNN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCCCEEEHHHHHCCCC
KENSELREHALQESINKSLSSIFRPEFLNRIDEVVKFDLLSIDQLQKIILLQINELKKLL
CCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LEQGININIDKKVILKIANDSYQPEYGARPLSRELRRQIENPLASRLLEENFKNKKNISV
HHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
KINPSKKDEILFKPS
EECCCCCCCEEECCC
>Mature Secondary Structure
MRETLTSNPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFTSSDFFAFIEKLSID
CCCCCCCCCHHHHCCCCEEEEEECHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC
QDTVLDITEDFLEETPTNDSDIFTIGEDLEILLDNANQIKTQWGSNLIEIPHLLIALGRD
CHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCC
ARIGNYVFQEGDLSVEQLEEELKIFPNINPRQNYLENKKNITNKENKSINKTDKKALIEK
CCCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
TKPIITTPKNNLKIETQHNSSQNALSLYGKDLTDSAKKGLLDPVVGRENEINNLMRVLSR
CCCEEECCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RSKNNPILIGYPGVGKTSIAKLLAQLIVERKVPDSLKNLKIISLDLGALVSGTKFRGQLE
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHCCEEEEEEHHHHHCCCHHHHHHH
ERLSLILNEIKDSNQGIILFIDEIHTIISSDRSTADISNILKPLLTEGELRCIGTTTPEK
HHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCHHH
FRENIERDQALNNCFQKILVNEPSVELTAQILKGIKKKYELHHGIRITEEAINSSARLAD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHH
RYISDKFLPDKAIDLIDEAAAQLKIEFNIKPQIILDEESKIYNIENKLKDLSPENIEDRE
HHHHHCCCCHHHHHHHHHHHHEEEEEEECCCEEEECCCCCEEEHHHHHHCCCCCCCHHHH
KLLERKELATDKLNAIINNWNNDQEKMEQLTFLLHEEDRLILEIEELNMNSEEINYFERL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
NNLEEELNEVEFEINNIESRFKNPQRDKKFNFKYQVEPDDIADVISKITGIPMAKVVSNE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCHHHHHHHH
RKKLVNLELEISEKVIGQKKAIEAVSSAIRRARVGMKNPKRPVGSFLFMGPTGVGKTELA
HHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHH
KSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYSVIL
HHHHHHHHCHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCEEEE
LDEIEKAHSEVFNVLLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKIILEDAKKIPNN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCCCEEEHHHHHCCCC
KENSELREHALQESINKSLSSIFRPEFLNRIDEVVKFDLLSIDQLQKIILLQINELKKLL
CCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LEQGININIDKKVILKIANDSYQPEYGARPLSRELRRQIENPLASRLLEENFKNKKNISV
HHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
KINPSKKDEILFKPS
EECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]