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The map label for this gene is def

Identifier: 123965312

GI number: 123965312

Start: 81376

End: 81987

Strand: Reverse

Name: def

Synonym: P9515_00771

Alternate gene names: 123965312

Gene position: 81987-81376 (Counterclockwise)

Preceding gene: 123965314

Following gene: 123965311

Centisome position: 4.81

GC content: 32.35

Gene sequence:

>612_bases
GTGGCAAATAATTTTTCACAGTTAGCAAAAAAATCTCGCAATGAACTTTCTTCCTCAAAAGTTAAGAAGGAGGTCATAGA
AAACCCTCCATTAGAAATATTTAAATTAGGTAATGATGTCTTAAGAACTAATGCCAAAAGAATTGGAAAGGTTGATTTAG
ATACAAGAAATTTAGCAAAAGACATGCTAAAAAGTATGTATTCTGCGAAAGGAATAGGTTTAGCCGCTCCACAAGTTGGT
ATTAGTAAAGAATTACTTGTAATTGATATAAATTTTGAAGACTCTGCAGCAGAGCCTCTTATTTTGATAAATCCAGAAAT
AACAGCTTTTGGTAACACTCTTAATTCTTATGAAGAAGGTTGTTTGAGTATACCTGGTGTATATTTAAATGTAGTCAGGC
CATCAACAATTAAATTAAGATTTAGTGATGAGATGGGAAGACCAAGAAAGATGAATGCTGATGGACTTTTAGCAAGATGC
ATTCAACATGAAGTGGACCATTTAAGGGGGGTTCTTTTTGTAGACAGAGTTACATCCAAAGAAGATTTAAAAACTGAACT
AAAAAAAGAAGGATTCCAAATGAAAGATGTGTTTCCAATAAGTCAATCTTAA

Upstream 100 bases:

>100_bases
AAAGTTTTGTGATTTATCATTTACTTTATTGCATTCAAAGTTTCAAATAAGTGTTAATATTTTTATATTCTAACTTTTTA
ATAACACCCTAATAGATATC

Downstream 100 bases:

>100_bases
TAATCAAAATTATTAATGACTCAAACCACAATCTTCAGCAAAATAATCAATGGTGAAATTCCCTGTGAAAAGCTTTATGA
AGACGAGCTTTGTATAGCAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MANNFSQLAKKSRNELSSSKVKKEVIENPPLEIFKLGNDVLRTNAKRIGKVDLDTRNLAKDMLKSMYSAKGIGLAAPQVG
ISKELLVIDINFEDSAAEPLILINPEITAFGNTLNSYEEGCLSIPGVYLNVVRPSTIKLRFSDEMGRPRKMNADGLLARC
IQHEVDHLRGVLFVDRVTSKEDLKTELKKEGFQMKDVFPISQS

Sequences:

>Translated_203_residues
MANNFSQLAKKSRNELSSSKVKKEVIENPPLEIFKLGNDVLRTNAKRIGKVDLDTRNLAKDMLKSMYSAKGIGLAAPQVG
ISKELLVIDINFEDSAAEPLILINPEITAFGNTLNSYEEGCLSIPGVYLNVVRPSTIKLRFSDEMGRPRKMNADGLLARC
IQHEVDHLRGVLFVDRVTSKEDLKTELKKEGFQMKDVFPISQS
>Mature_202_residues
ANNFSQLAKKSRNELSSSKVKKEVIENPPLEIFKLGNDVLRTNAKRIGKVDLDTRNLAKDMLKSMYSAKGIGLAAPQVGI
SKELLVIDINFEDSAAEPLILINPEITAFGNTLNSYEEGCLSIPGVYLNVVRPSTIKLRFSDEMGRPRKMNADGLLARCI
QHEVDHLRGVLFVDRVTSKEDLKTELKKEGFQMKDVFPISQS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=170, Percent_Identity=27.6470588235294, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=42.9530201342282, Blast_Score=116, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24645728, Length=175, Percent_Identity=33.1428571428571, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24645726, Length=169, Percent_Identity=29.585798816568, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_PROM5 (A2BU25)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010393.1
- ProteinModelPortal:   A2BU25
- SMR:   A2BU25
- STRING:   A2BU25
- GeneID:   4719954
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_00771
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   LMDISGG
- ProtClustDB:   PRK00150
- BioCyc:   PMAR167542:P9515ORF_0080-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 22636; Mature: 22504

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: NA

Important sites: ACT_SITE 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNFSQLAKKSRNELSSSKVKKEVIENPPLEIFKLGNDVLRTNAKRIGKVDLDTRNLAK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
DMLKSMYSAKGIGLAAPQVGISKELLVIDINFEDSAAEPLILINPEITAFGNTLNSYEEG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCHHHHCCHHHHHHHC
CLSIPGVYLNVVRPSTIKLRFSDEMGRPRKMNADGLLARCIQHEVDHLRGVLFVDRVTSK
CCCCCCEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCH
EDLKTELKKEGFQMKDVFPISQS
HHHHHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure 
ANNFSQLAKKSRNELSSSKVKKEVIENPPLEIFKLGNDVLRTNAKRIGKVDLDTRNLAK
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCCCHHHHHH
DMLKSMYSAKGIGLAAPQVGISKELLVIDINFEDSAAEPLILINPEITAFGNTLNSYEEG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCHHHHCCHHHHHHHC
CLSIPGVYLNVVRPSTIKLRFSDEMGRPRKMNADGLLARCIQHEVDHLRGVLFVDRVTSK
CCCCCCEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCH
EDLKTELKKEGFQMKDVFPISQS
HHHHHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA