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The map label for this gene is thiG

Identifier: 121999177

GI number: 121999177

Start: 2636566

End: 2637354

Strand: Reverse

Name: thiG

Synonym: Hhal_2399

Alternate gene names: 121999177

Gene position: 2637354-2636566 (Counterclockwise)

Preceding gene: 121999178

Following gene: 121999176

Centisome position: 98.47

GC content: 68.19

Gene sequence:

>789_bases
ATGGCGGATCGCGATCCCCTGGTCATCGCCGGGCGTGAGTACCACTCGCGTCTGCTGGTCGGTACCGGCAAGTACCGTGA
TATGGAGCAGACCCGCGCGGCCATCGAGGCGAGCGGTGCAGAGATCGTCACCATCGCCCTGCGTCGCAGCAACATCGGCC
AGAATCCCGACGAGCCGAACCTGCTCGACGCCGTACCGCCGGATCGCTACACCATCCTGCCCAATACCGCCGGCTGCTAT
ACGGTCAAGGATGCGGTGCGCACCTGCCGGTTGGGCCGCGAGCTGCTTGACGGCCACAACCTGGTCAAGCTCGAGGTCAT
CGGCGACGAGCGCACCCTCTACCCGGATGTGCCCGGGACCCTGGAGGCCGCCGAGCAGCTGGTGGCCGAGGGCTTCGAGG
TCATGGCCTATACCAGCGACGACCCCATCACCGCCAAACGGCTCGAAGAGATCGGCTGCGTGGCCGTGATGCCGCTGGCC
GCCCCCATCGGTTCGGGGCTCGGGATCCAGAACCGCTACAACATCCTGGAGATCGTCGACCGCCTGGAGGTCCCGGTCCT
GGTGGACGCCGGGGTGGGGACCGCCTCCGACGTGGCCGTGGCCATGGAGCTCGGCTGTGACGCCGTGTTGCTCAATACGG
CCATCGCCGGTGCGCGGGATCCGGTGCTCATGGCCTCGGCCATGCGCAAGGGCGTCGAGGCCGGGCGCGAGGCGTACCGT
GCCGGTCGCATCCCCAAGAAGCGCTACGCCGCGGCGTCGTCGCCCGAGGAAGGGACCTTCTTCGAGTGA

Upstream 100 bases:

>100_bases
CGGCGACCACCAACTGGCCGATGGCGATCGCGTGGAGGTCATCCAGGCCATCGGCGGCGGCTGACCCGAGTCCCGAAACA
GCACGGAGTCCAAGAGAAAG

Downstream 100 bases:

>100_bases
GCCAGCCGCGACAGACCCGCACATTCGTCCGCCGCGAGGGGCGCCTGACCCAGGGCCAGCAGCGGGCCCTGGAGGGGCTC
TGGCCGCAATTCGGGGTCGA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MADRDPLVIAGREYHSRLLVGTGKYRDMEQTRAAIEASGAEIVTIALRRSNIGQNPDEPNLLDAVPPDRYTILPNTAGCY
TVKDAVRTCRLGRELLDGHNLVKLEVIGDERTLYPDVPGTLEAAEQLVAEGFEVMAYTSDDPITAKRLEEIGCVAVMPLA
APIGSGLGIQNRYNILEIVDRLEVPVLVDAGVGTASDVAVAMELGCDAVLLNTAIAGARDPVLMASAMRKGVEAGREAYR
AGRIPKKRYAAASSPEEGTFFE

Sequences:

>Translated_262_residues
MADRDPLVIAGREYHSRLLVGTGKYRDMEQTRAAIEASGAEIVTIALRRSNIGQNPDEPNLLDAVPPDRYTILPNTAGCY
TVKDAVRTCRLGRELLDGHNLVKLEVIGDERTLYPDVPGTLEAAEQLVAEGFEVMAYTSDDPITAKRLEEIGCVAVMPLA
APIGSGLGIQNRYNILEIVDRLEVPVLVDAGVGTASDVAVAMELGCDAVLLNTAIAGARDPVLMASAMRKGVEAGREAYR
AGRIPKKRYAAASSPEEGTFFE
>Mature_261_residues
ADRDPLVIAGREYHSRLLVGTGKYRDMEQTRAAIEASGAEIVTIALRRSNIGQNPDEPNLLDAVPPDRYTILPNTAGCYT
VKDAVRTCRLGRELLDGHNLVKLEVIGDERTLYPDVPGTLEAAEQLVAEGFEVMAYTSDDPITAKRLEEIGCVAVMPLAA
PIGSGLGIQNRYNILEIVDRLEVPVLVDAGVGTASDVAVAMELGCDAVLLNTAIAGARDPVLMASAMRKGVEAGREAYRA
GRIPKKRYAAASSPEEGTFFE

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=256, Percent_Identity=46.484375, Blast_Score=206, Evalue=9e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28124; Mature: 27993

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRDPLVIAGREYHSRLLVGTGKYRDMEQTRAAIEASGAEIVTIALRRSNIGQNPDEPN
CCCCCCEEEECCHHCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCC
LLDAVPPDRYTILPNTAGCYTVKDAVRTCRLGRELLDGHNLVKLEVIGDERTLYPDVPGT
CCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCH
LEAAEQLVAEGFEVMAYTSDDPITAKRLEEIGCVAVMPLAAPIGSGLGIQNRYNILEIVD
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCEEEEHHHHHCCCCCCCCCCHHHHHHHH
RLEVPVLVDAGVGTASDVAVAMELGCDAVLLNTAIAGARDPVLMASAMRKGVEAGREAYR
HCCCCEEEECCCCCHHHHHHHHHHCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AGRIPKKRYAAASSPEEGTFFE
HCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ADRDPLVIAGREYHSRLLVGTGKYRDMEQTRAAIEASGAEIVTIALRRSNIGQNPDEPN
CCCCCEEEECCHHCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCC
LLDAVPPDRYTILPNTAGCYTVKDAVRTCRLGRELLDGHNLVKLEVIGDERTLYPDVPGT
CCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCH
LEAAEQLVAEGFEVMAYTSDDPITAKRLEEIGCVAVMPLAAPIGSGLGIQNRYNILEIVD
HHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCEEEEHHHHHCCCCCCCCCCHHHHHHHH
RLEVPVLVDAGVGTASDVAVAMELGCDAVLLNTAIAGARDPVLMASAMRKGVEAGREAYR
HCCCCEEEECCCCCHHHHHHHHHHCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
AGRIPKKRYAAASSPEEGTFFE
HCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA