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The map label for this gene is trmB

Identifier: 121999176

GI number: 121999176

Start: 2635874

End: 2636569

Strand: Reverse

Name: trmB

Synonym: Hhal_2398

Alternate gene names: 121999176

Gene position: 2636569-2635874 (Counterclockwise)

Preceding gene: 121999177

Following gene: 121999174

Centisome position: 98.44

GC content: 72.27

Gene sequence:

>696_bases
GTGAGCCAGCCGCGACAGACCCGCACATTCGTCCGCCGCGAGGGGCGCCTGACCCAGGGCCAGCAGCGGGCCCTGGAGGG
GCTCTGGCCGCAATTCGGGGTCGATGTCCCCGAGCGCGGGGTGATCGACCTCGACGGCCTGTTCGGTCGTGCCGCGCCGC
GGGTGCTGGATATCGGCTTTGGCGACGGCGAGGCCCTGGTGGAGATGGCTGCCGCCGACCCGGAGCGCGATTACCTGGGG
GTCGAGGTCCACCGGCCGGGGGTGGGGCACTGCCTGCTCTGCGCCGAGCAGGCCGGGCTCGACAACCTCCGCGTGGCGAC
GGTGGATGCCGTCGAGCTGGTGCGCCACCACCTGCCCGGGCCCAGCCTGGAGACCGTGCAGATCTTCTTTCCGGATCCCT
GGCCGAAGAAGCGCCACCACAAGCGGCGGATCATCCAGCCCGCCTTCCTCGACCTCCTCGCCGAGCGATTGGTGCCCGGG
GGGGCGCTGCACCTGGCCACCGACTGGGCGGACTACGCCGAGTGGATGCTCGACACGCTGGAGGCCGACGCGCGCTTCGA
GAACACCTGTGGTCCGCGCGCCTTCGTGCCGCCTCCGCCGCCGCGCCCGCAGACCAAGTTCGAGCGCCGCGGCCTGCGCA
AGGGGCACCAGGTCCACGATCTGATCTACCGTCTGCGGCCGGACACCGCCGGCTAG

Upstream 100 bases:

>100_bases
GCAAGGGCGTCGAGGCCGGGCGCGAGGCGTACCGTGCCGGTCGCATCCCCAAGAAGCGCTACGCCGCGGCGTCGTCGCCC
GAGGAAGGGACCTTCTTCGA

Downstream 100 bases:

>100_bases
CCGGCCCGCCTCGGCGGGCTGGCTAGCCGCGCGTCGGGGGATCTTCGCTGAGCCCGCTCTGGCGCAGCAGCGGCTCGATG
CTCGGCGAACGGCCGCGGAA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSQPRQTRTFVRREGRLTQGQQRALEGLWPQFGVDVPERGVIDLDGLFGRAAPRVLDIGFGDGEALVEMAAADPERDYLG
VEVHRPGVGHCLLCAEQAGLDNLRVATVDAVELVRHHLPGPSLETVQIFFPDPWPKKRHHKRRIIQPAFLDLLAERLVPG
GALHLATDWADYAEWMLDTLEADARFENTCGPRAFVPPPPPRPQTKFERRGLRKGHQVHDLIYRLRPDTAG

Sequences:

>Translated_231_residues
MSQPRQTRTFVRREGRLTQGQQRALEGLWPQFGVDVPERGVIDLDGLFGRAAPRVLDIGFGDGEALVEMAAADPERDYLG
VEVHRPGVGHCLLCAEQAGLDNLRVATVDAVELVRHHLPGPSLETVQIFFPDPWPKKRHHKRRIIQPAFLDLLAERLVPG
GALHLATDWADYAEWMLDTLEADARFENTCGPRAFVPPPPPRPQTKFERRGLRKGHQVHDLIYRLRPDTAG
>Mature_230_residues
SQPRQTRTFVRREGRLTQGQQRALEGLWPQFGVDVPERGVIDLDGLFGRAAPRVLDIGFGDGEALVEMAAADPERDYLGV
EVHRPGVGHCLLCAEQAGLDNLRVATVDAVELVRHHLPGPSLETVQIFFPDPWPKKRHHKRRIIQPAFLDLLAERLVPGG
ALHLATDWADYAEWMLDTLEADARFENTCGPRAFVPPPPPRPQTKFERRGLRKGHQVHDLIYRLRPDTAG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=219, Percent_Identity=52.0547945205479, Blast_Score=229, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6320000, Length=141, Percent_Identity=30.4964539007092, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_HALHL (A1WZP9)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003963.1
- ProteinModelPortal:   A1WZP9
- SMR:   A1WZP9
- STRING:   A1WZP9
- GeneID:   4710033
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_2398
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   A1WZP9
- ProtClustDB:   PRK00121
- BioCyc:   HHAL349124:HHAL_2398-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25960; Mature: 25829

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 82-82 BINDING 109-109 BINDING 132-132 BINDING 136-136 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPRQTRTFVRREGRLTQGQQRALEGLWPQFGVDVPERGVIDLDGLFGRAAPRVLDIGF
CCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCCCCEEECCCHHHCCCCEEEEECC
GDGEALVEMAAADPERDYLGVEVHRPGVGHCLLCAEQAGLDNLRVATVDAVELVRHHLPG
CCHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEHHHCCCCCEEEEHHHHHHHHHHHCCC
PSLETVQIFFPDPWPKKRHHKRRIIQPAFLDLLAERLVPGGALHLATDWADYAEWMLDTL
CCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
EADARFENTCGPRAFVPPPPPRPQTKFERRGLRKGHQVHDLIYRLRPDTAG
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQPRQTRTFVRREGRLTQGQQRALEGLWPQFGVDVPERGVIDLDGLFGRAAPRVLDIGF
CCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCCCCEEECCCHHHCCCCEEEEECC
GDGEALVEMAAADPERDYLGVEVHRPGVGHCLLCAEQAGLDNLRVATVDAVELVRHHLPG
CCHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEHHHCCCCCEEEEHHHHHHHHHHHCCC
PSLETVQIFFPDPWPKKRHHKRRIIQPAFLDLLAERLVPGGALHLATDWADYAEWMLDTL
CCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
EADARFENTCGPRAFVPPPPPRPQTKFERRGLRKGHQVHDLIYRLRPDTAG
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA