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The map label for this gene is rsmB [H]

Identifier: 121999099

GI number: 121999099

Start: 2546549

End: 2547838

Strand: Reverse

Name: rsmB [H]

Synonym: Hhal_2321

Alternate gene names: 121999099

Gene position: 2547838-2546549 (Counterclockwise)

Preceding gene: 121999100

Following gene: 121999098

Centisome position: 95.12

GC content: 73.33

Gene sequence:

>1290_bases
GTGAGTACCCCCCCGCGGGTCGCCGCGGTGCACGTCCTTGAACGGGTGCTGGAGCGCGGCGAGACCCTGGATGAGGCCCT
GGAGGCCCAGTTTTCCCGGGTATCCGAGCGCAATCGGTCGTTGCTTCAGGCCCTGGTCTACGGCGCGCTGCGCTGGCTGA
CCCGGCTCGAGGCCCAGGTGGCGACCCTGACCCCGCGCGACGACTGGCGCGCCGATCCGCTGCTACGTGGTCTGCTGGTG
ATCGGGGCCTGGGAGGCCCAGGGGCTTGCCACCCCAGCCCATGCGGCGGTCTCCGAGGCCGTCGATGCGGCCCGACGGCT
GCGTCGGGCGCGCGCGGCCGGCATGGTGAACGCGGTGCTGCGCAAGCTGCACAAGGCCACCCCACCCGGGCCGGCAGACG
AAGCCGCCCGCTACGCCCTGCCGCCGTGGCTGCTGGACCATCTACGCCGCGCCTGGCCGGAGGATTGGCCGGCGGTGGCC
GAGGCCGGGAACGCTCACCCGCCAATGACCCTGCGCTTCGACCGGAACCGCATCTCCCGCGAGGCGTGCCTGCAATCGCT
GGCCGAGCAGGAGATCCCGGCGCACCCCGGCGAGGTCGCGCCGAGCGCCGCGACCCTCCATGCCCCGGTACCCGTGGCCC
GCCTGCCCGGCTTCGCCGAGGGGTGGTTGTCGGTGCAGGATGAGGCCGCGCAGCTCGCCGCCCCGCTTCTCGACCCGCAA
CCCGGCGACCGGGTGCTCGATGCCTGTGCCGCACCGGGTGGTAAGACCCTGCACCTGCTCGAGCACACGCCGACGGCGGC
GGTGACCGCCCTCGACCGTTCGTCACGCCGGCTGCGCCAGGTACGCGATAACCTCGCGCGTGGCGGCTACGAGGCCCAAT
GCCTGGCCGCCGATGCCGCCGACCCCGAAGCGTGGTGGGACGGTGAACCGTTTCAGCGCATCCTCCTCGACGCCCCGTGC
ACGGGCAGCGGCGTGATCCGCCGACACCCCGATATCAAGTGGCTGCGCGGGGTCGACGACCCGGCGCGCATGGCGGCGGC
GCAGCGCCACCTGCTCGCCGCCCTGTGGCGCGTGCTGGCGCCGGGCGGGCGGTTGCTCTACGCCACCTGCTCGATCTTCC
CCGAGGAGAACGAGCAGGTGGTGGCCGGTTTCCTGGCCGAGCACGCCGATGCCAAACCGGGGCCGTTGGCGCCGGTGGGC
CGCTGCACCGGGTCCGGGTGCCAGATCCTGCCCGGCGAGCACGGGATGGATGGGTTCTTCTACGCCTGCCTCGAGCGGAG
TGCCGCATGA

Upstream 100 bases:

>100_bases
GGCGTCTTGAGATCACCCGGCTGAAGCCCGCCGGTGGGCGGGAACAGACGGCTGCCGATTACCTCAACGGGCGCCGCCTG
GTGCCGGGAGAGCCGGTTCA

Downstream 100 bases:

>100_bases
CGGATGCGATGCCGGTCGACGGGATCCAGCGCGTCCACGACTGGCTGTTGACCTGGCAGGAGCGTGTCTGCCGGTTCGTT
GAAGGTTGGGACGGTGGCGG

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSTPPRVAAVHVLERVLERGETLDEALEAQFSRVSERNRSLLQALVYGALRWLTRLEAQVATLTPRDDWRADPLLRGLLV
IGAWEAQGLATPAHAAVSEAVDAARRLRRARAAGMVNAVLRKLHKATPPGPADEAARYALPPWLLDHLRRAWPEDWPAVA
EAGNAHPPMTLRFDRNRISREACLQSLAEQEIPAHPGEVAPSAATLHAPVPVARLPGFAEGWLSVQDEAAQLAAPLLDPQ
PGDRVLDACAAPGGKTLHLLEHTPTAAVTALDRSSRRLRQVRDNLARGGYEAQCLAADAADPEAWWDGEPFQRILLDAPC
TGSGVIRRHPDIKWLRGVDDPARMAAAQRHLLAALWRVLAPGGRLLYATCSIFPEENEQVVAGFLAEHADAKPGPLAPVG
RCTGSGCQILPGEHGMDGFFYACLERSAA

Sequences:

>Translated_429_residues
MSTPPRVAAVHVLERVLERGETLDEALEAQFSRVSERNRSLLQALVYGALRWLTRLEAQVATLTPRDDWRADPLLRGLLV
IGAWEAQGLATPAHAAVSEAVDAARRLRRARAAGMVNAVLRKLHKATPPGPADEAARYALPPWLLDHLRRAWPEDWPAVA
EAGNAHPPMTLRFDRNRISREACLQSLAEQEIPAHPGEVAPSAATLHAPVPVARLPGFAEGWLSVQDEAAQLAAPLLDPQ
PGDRVLDACAAPGGKTLHLLEHTPTAAVTALDRSSRRLRQVRDNLARGGYEAQCLAADAADPEAWWDGEPFQRILLDAPC
TGSGVIRRHPDIKWLRGVDDPARMAAAQRHLLAALWRVLAPGGRLLYATCSIFPEENEQVVAGFLAEHADAKPGPLAPVG
RCTGSGCQILPGEHGMDGFFYACLERSAA
>Mature_428_residues
STPPRVAAVHVLERVLERGETLDEALEAQFSRVSERNRSLLQALVYGALRWLTRLEAQVATLTPRDDWRADPLLRGLLVI
GAWEAQGLATPAHAAVSEAVDAARRLRRARAAGMVNAVLRKLHKATPPGPADEAARYALPPWLLDHLRRAWPEDWPAVAE
AGNAHPPMTLRFDRNRISREACLQSLAEQEIPAHPGEVAPSAATLHAPVPVARLPGFAEGWLSVQDEAAQLAAPLLDPQP
GDRVLDACAAPGGKTLHLLEHTPTAAVTALDRSSRRLRQVRDNLARGGYEAQCLAADAADPEAWWDGEPFQRILLDAPCT
GSGVIRRHPDIKWLRGVDDPARMAAAQRHLLAALWRVLAPGGRLLYATCSIFPEENEQVVAGFLAEHADAKPGPLAPVGR
CTGSGCQILPGEHGMDGFFYACLERSAA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=326, Percent_Identity=30.6748466257669, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI76150623, Length=326, Percent_Identity=30.6748466257669, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI32698918, Length=227, Percent_Identity=33.0396475770925, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI270288816, Length=233, Percent_Identity=36.0515021459227, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI23199998, Length=233, Percent_Identity=36.0515021459227, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI8922322, Length=233, Percent_Identity=36.0515021459227, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI270288818, Length=233, Percent_Identity=36.0515021459227, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI11545785, Length=230, Percent_Identity=26.5217391304348, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI301336155, Length=189, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI39995082, Length=189, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI40316918, Length=228, Percent_Identity=31.140350877193, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI2367212, Length=422, Percent_Identity=41.4691943127962, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87081985, Length=225, Percent_Identity=32.4444444444444, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536757, Length=320, Percent_Identity=29.6875, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71998419, Length=275, Percent_Identity=29.0909090909091, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115534021, Length=275, Percent_Identity=28.7272727272727, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25143471, Length=182, Percent_Identity=33.5164835164835, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324268, Length=315, Percent_Identity=26.6666666666667, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319447, Length=288, Percent_Identity=27.4305555555556, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI22024126, Length=315, Percent_Identity=27.6190476190476, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356513, Length=175, Percent_Identity=36, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24668781, Length=175, Percent_Identity=36, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355201, Length=203, Percent_Identity=31.0344827586207, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920494, Length=165, Percent_Identity=31.5151515151515, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46451; Mature: 46320

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPPRVAAVHVLERVLERGETLDEALEAQFSRVSERNRSLLQALVYGALRWLTRLEAQV
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLTPRDDWRADPLLRGLLVIGAWEAQGLATPAHAAVSEAVDAARRLRRARAAGMVNAVL
HCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKLHKATPPGPADEAARYALPPWLLDHLRRAWPEDWPAVAEAGNAHPPMTLRFDRNRISR
HHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECHHHHHH
EACLQSLAEQEIPAHPGEVAPSAATLHAPVPVARLPGFAEGWLSVQDEAAQLAAPLLDPQ
HHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
PGDRVLDACAAPGGKTLHLLEHTPTAAVTALDRSSRRLRQVRDNLARGGYEAQCLAADAA
CCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
DPEAWWDGEPFQRILLDAPCTGSGVIRRHPDIKWLRGVDDPARMAAAQRHLLAALWRVLA
CCCCCCCCCHHHHHHHCCCCCCCCCEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHC
PGGRLLYATCSIFPEENEQVVAGFLAEHADAKPGPLAPVGRCTGSGCQILPGEHGMDGFF
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHH
YACLERSAA
HHHHHHCCC
>Mature Secondary Structure 
STPPRVAAVHVLERVLERGETLDEALEAQFSRVSERNRSLLQALVYGALRWLTRLEAQV
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLTPRDDWRADPLLRGLLVIGAWEAQGLATPAHAAVSEAVDAARRLRRARAAGMVNAVL
HCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKLHKATPPGPADEAARYALPPWLLDHLRRAWPEDWPAVAEAGNAHPPMTLRFDRNRISR
HHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEECHHHHHH
EACLQSLAEQEIPAHPGEVAPSAATLHAPVPVARLPGFAEGWLSVQDEAAQLAAPLLDPQ
HHHHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
PGDRVLDACAAPGGKTLHLLEHTPTAAVTALDRSSRRLRQVRDNLARGGYEAQCLAADAA
CCHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
DPEAWWDGEPFQRILLDAPCTGSGVIRRHPDIKWLRGVDDPARMAAAQRHLLAALWRVLA
CCCCCCCCCHHHHHHHCCCCCCCCCEECCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHC
PGGRLLYATCSIFPEENEQVVAGFLAEHADAKPGPLAPVGRCTGSGCQILPGEHGMDGFF
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCHHHH
YACLERSAA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]