Click here to switch to the map view.

The map label for this gene is mrcA [H]

Identifier: 121999032

GI number: 121999032

Start: 2473084

End: 2475495

Strand: Reverse

Name: mrcA [H]

Synonym: Hhal_2253

Alternate gene names: 121999032

Gene position: 2475495-2473084 (Counterclockwise)

Preceding gene: 121999036

Following gene: 121999031

Centisome position: 92.42

GC content: 68.91

Gene sequence:

>2412_bases
ATGAAGCGATTCCTGCGTATCCTCCTTTACAGCACCCTCACCCTGGTCGGTTCCGGCGCCGTGCTGGGTGCCGCGGCGGT
GGCTTATGCCCTGCTGGTGCTTGCTCCGCAGTTGCCCGATGCGGAGTCGCTGCGCGACGTGCGCTTCCAGCAGCCGCTGC
GTGTTGTCAGCGCCGAGGGCGAGCTGATCGCCGAGTACGGCGAGCAGCGCCGCCAACCGGAGGATATCGACGACATCCCC
ACGGACCTGTTGCTGGCGTTCATCGCCGCTGAGGACGACCGCTTCTACGAGCACCCGGGGGTCGACTATCAGGGCCTGGC
GCGGGCGGTGAGCCACGTGGCCCGCGAGCGCTCCTTCGGCCCGGGGGGGTCGACCATCACCATGCAGGTTGCGCGCAATT
TCTTCCTCTCGCGCGAAGTCACCATCCTGCGCAAGGTCAACGAGATCCTGCTGGCGCTGCAGATCGAGCGCACCCTGTCC
AAGGAGGAGATCCTCGAGCTCTATCTCAACAAGATCTACCTGGGCAATCGCGCCTACGGCGTTGCTGCCGCCGCCCAGGT
CTATTACGGCAAGGACCTGGATGAGCTGGATCTGGCCCAGATGGCGATGATTGCCGGTCTGCCCAAGGCGCCCTCGGCCG
CCAACCCCATCCGCAATCCCCGGTTGGCCCTGCAGCGGCGTGCCTACGTCCTTGGGCGGATGCTCAACGAGGGCTTCATC
AGCCCGGAGCGTTATCAGGAGGCTATGGCCGCACCGGTAACCGCGGAACTGCACGCCCCGCGCAGCCGTGTGGATGCCCG
TTACGCCGGGGAGATGGTCCGCCGCACCATGACCGAGCGCTACGGGGCCGAGGAGGCGTATACCGGCGGCTATACGGTCT
ATACCACCATCGAGAAGGACCGCCAGGAGGCGGCCAACCGTGCCCTGCGCAGCGGGTTGCGCGATTACGACCAGCGCCAC
GGCTACCGGGGCCCCGAAGGCAGCATGGACGCGCAGGCCGTCGGCGACCCGGCGGCCATGAACGAGGTGCTGGGGGAACA
CCCGCCGTTCGGCGGCCTGCAAGCGGCCGTGGTGCTCGCCGTAGAGCCCGAGGCGGCCTGGGTCTGGCCCCGGGGCGGGC
GGGTGCTGCAGGTGGACTTCGAGACCATGGAGTGGGCGCGTGCCTACCGGGGTGCCAATCGGCGCGGCCCGGCCCCGGAA
GAGGCGGGTGACGTGCTCGAGGTCGGCGATATCGTGCGGGTGATGCCGCAGGGCGAGGGGCGGGCGGCGCTCAGCGCCGT
GCCCGACGTCTCCGGCGCGCTGGTCGCCCAGGATCCGCTGGACGGGCGCGTGCGGGCCCTGGCCGGTGGGTTCGATTTCC
GCCTCAGCTCCTTCAACCGGGCGGTCCAGGCGCGGCGTCAGACGGGGTCGGTCTTCAAGCCGTTCATCTACTCGGCGGCC
CTGGAAGGCGGCTACACGCCGGCCACCCTGGTCAACGACGCCCCGGTGGTCTTCCGGGACGACGCCCTGGAGAGCTACTG
GCGCCCCGGCAACTACTCGGGGCGGTTCTATGGCCCGACCCGGCTGCGCGAGGCCCTGGTCTTCTCGCGCAACCTGGTGT
CGATCCGGGTGCTGCGCGATGTGGGGATCGACCCGACTATCGCCCATGCCGTGCGTATGGGCTTCCCGCCGGAGAGCCTG
CCGCGGGATCTCTCCCTGGCGCTGGGCAGCAATAGCGCCACTCCGCTGCAGATGTCGAACGCCTTTGCGGCGTTCGCCAA
CGGTGGCTACCGCGTCTTCCCCAATATCATCGAGCGGGTGGAAGACGGCGCCGGTGAAGTGGTCTTCGAGCCCGAGTTTC
CGGAGGCGTGTGAGCTCTGCCGTGACGACCTGCCCATGGCCTCGGTGCCCGGTGACCGCACTGGCCTGTTCGATCTGCCG
CCCCCGGCAGAGCGTGTCCTGCCGGAAGACAACGCCTGGCTGATCAGCGATATGCTCGCCGACGTCATTGAGCGTGGCAC
CGGACGGGGCGTGCGGCGGGTGCTCGATCGCGGGGACCTGGCCGGCAAGACCGGTACCACCAACGATCTGCGCGACGCCT
GGTTCGTGGGGTACAACCAGGATCTGGTGGCCACGGCGTGGATCGGTTTCGACGACCAGCGCAGCCTGGGGCGTGGCGAA
ACCGGTGCTTCGGCCGCCCTGCCCATCTGGGCCCGCTTTATGTCCGTAGCCCTCGACGGGGTCGAGGAGCGCCCCATGCC
GCGCCCGGGCGGCTTGGTCACGGTGCGCATCGACCCGGAGTCGGGGCGGGCCACCAGCTCCGAGAACCCTGACGCCATTT
TCGAGACCTTCCGCGAGGAGACCGTCCCGGTCCGTGACGACAGCGATCGCCGGCAGCGTGGTGATGAGCACACGGAGCGG
GAACTCTTCTAG

Upstream 100 bases:

>100_bases
AGGCATTGACGCCCCTACTATAAACCGGGAACTCGCCCGTGCAAGAGGTGGTCAGTATACTGCCCACAGAGTTTGCCAAG
GCGCCCGCGCCCCGTCCCCG

Downstream 100 bases:

>100_bases
GCGGGTGCCAACAGACTGCCGGAGCAGGGGGAGGAGCTATGGGACGCAGAGGAGCCAACCATCAACGCGATGAGCGCATG
CGCTTGAGTCTGATTCGCGA

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MKRFLRILLYSTLTLVGSGAVLGAAAVAYALLVLAPQLPDAESLRDVRFQQPLRVVSAEGELIAEYGEQRRQPEDIDDIP
TDLLLAFIAAEDDRFYEHPGVDYQGLARAVSHVARERSFGPGGSTITMQVARNFFLSREVTILRKVNEILLALQIERTLS
KEEILELYLNKIYLGNRAYGVAAAAQVYYGKDLDELDLAQMAMIAGLPKAPSAANPIRNPRLALQRRAYVLGRMLNEGFI
SPERYQEAMAAPVTAELHAPRSRVDARYAGEMVRRTMTERYGAEEAYTGGYTVYTTIEKDRQEAANRALRSGLRDYDQRH
GYRGPEGSMDAQAVGDPAAMNEVLGEHPPFGGLQAAVVLAVEPEAAWVWPRGGRVLQVDFETMEWARAYRGANRRGPAPE
EAGDVLEVGDIVRVMPQGEGRAALSAVPDVSGALVAQDPLDGRVRALAGGFDFRLSSFNRAVQARRQTGSVFKPFIYSAA
LEGGYTPATLVNDAPVVFRDDALESYWRPGNYSGRFYGPTRLREALVFSRNLVSIRVLRDVGIDPTIAHAVRMGFPPESL
PRDLSLALGSNSATPLQMSNAFAAFANGGYRVFPNIIERVEDGAGEVVFEPEFPEACELCRDDLPMASVPGDRTGLFDLP
PPAERVLPEDNAWLISDMLADVIERGTGRGVRRVLDRGDLAGKTGTTNDLRDAWFVGYNQDLVATAWIGFDDQRSLGRGE
TGASAALPIWARFMSVALDGVEERPMPRPGGLVTVRIDPESGRATSSENPDAIFETFREETVPVRDDSDRRQRGDEHTER
ELF

Sequences:

>Translated_803_residues
MKRFLRILLYSTLTLVGSGAVLGAAAVAYALLVLAPQLPDAESLRDVRFQQPLRVVSAEGELIAEYGEQRRQPEDIDDIP
TDLLLAFIAAEDDRFYEHPGVDYQGLARAVSHVARERSFGPGGSTITMQVARNFFLSREVTILRKVNEILLALQIERTLS
KEEILELYLNKIYLGNRAYGVAAAAQVYYGKDLDELDLAQMAMIAGLPKAPSAANPIRNPRLALQRRAYVLGRMLNEGFI
SPERYQEAMAAPVTAELHAPRSRVDARYAGEMVRRTMTERYGAEEAYTGGYTVYTTIEKDRQEAANRALRSGLRDYDQRH
GYRGPEGSMDAQAVGDPAAMNEVLGEHPPFGGLQAAVVLAVEPEAAWVWPRGGRVLQVDFETMEWARAYRGANRRGPAPE
EAGDVLEVGDIVRVMPQGEGRAALSAVPDVSGALVAQDPLDGRVRALAGGFDFRLSSFNRAVQARRQTGSVFKPFIYSAA
LEGGYTPATLVNDAPVVFRDDALESYWRPGNYSGRFYGPTRLREALVFSRNLVSIRVLRDVGIDPTIAHAVRMGFPPESL
PRDLSLALGSNSATPLQMSNAFAAFANGGYRVFPNIIERVEDGAGEVVFEPEFPEACELCRDDLPMASVPGDRTGLFDLP
PPAERVLPEDNAWLISDMLADVIERGTGRGVRRVLDRGDLAGKTGTTNDLRDAWFVGYNQDLVATAWIGFDDQRSLGRGE
TGASAALPIWARFMSVALDGVEERPMPRPGGLVTVRIDPESGRATSSENPDAIFETFREETVPVRDDSDRRQRGDEHTER
ELF
>Mature_803_residues
MKRFLRILLYSTLTLVGSGAVLGAAAVAYALLVLAPQLPDAESLRDVRFQQPLRVVSAEGELIAEYGEQRRQPEDIDDIP
TDLLLAFIAAEDDRFYEHPGVDYQGLARAVSHVARERSFGPGGSTITMQVARNFFLSREVTILRKVNEILLALQIERTLS
KEEILELYLNKIYLGNRAYGVAAAAQVYYGKDLDELDLAQMAMIAGLPKAPSAANPIRNPRLALQRRAYVLGRMLNEGFI
SPERYQEAMAAPVTAELHAPRSRVDARYAGEMVRRTMTERYGAEEAYTGGYTVYTTIEKDRQEAANRALRSGLRDYDQRH
GYRGPEGSMDAQAVGDPAAMNEVLGEHPPFGGLQAAVVLAVEPEAAWVWPRGGRVLQVDFETMEWARAYRGANRRGPAPE
EAGDVLEVGDIVRVMPQGEGRAALSAVPDVSGALVAQDPLDGRVRALAGGFDFRLSSFNRAVQARRQTGSVFKPFIYSAA
LEGGYTPATLVNDAPVVFRDDALESYWRPGNYSGRFYGPTRLREALVFSRNLVSIRVLRDVGIDPTIAHAVRMGFPPESL
PRDLSLALGSNSATPLQMSNAFAAFANGGYRVFPNIIERVEDGAGEVVFEPEFPEACELCRDDLPMASVPGDRTGLFDLP
PPAERVLPEDNAWLISDMLADVIERGTGRGVRRVLDRGDLAGKTGTTNDLRDAWFVGYNQDLVATAWIGFDDQRSLGRGE
TGASAALPIWARFMSVALDGVEERPMPRPGGLVTVRIDPESGRATSSENPDAIFETFREETVPVRDDSDRRQRGDEHTER
ELF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=839, Percent_Identity=41.1203814064362, Blast_Score=599, Evalue=1e-172,
Organism=Escherichia coli, GI1786343, Length=328, Percent_Identity=29.5731707317073, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788867, Length=245, Percent_Identity=36.734693877551, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1789601, Length=164, Percent_Identity=37.1951219512195, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 88342; Mature: 88342

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFLRILLYSTLTLVGSGAVLGAAAVAYALLVLAPQLPDAESLRDVRFQQPLRVVSAEG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHCCCCC
ELIAEYGEQRRQPEDIDDIPTDLLLAFIAAEDDRFYEHPGVDYQGLARAVSHVARERSFG
HHHHHHHHHCCCCCCCHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
PGGSTITMQVARNFFLSREVTILRKVNEILLALQIERTLSKEEILELYLNKIYLGNRAYG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCHH
VAAAAQVYYGKDLDELDLAQMAMIAGLPKAPSAANPIRNPRLALQRRAYVLGRMLNEGFI
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
SPERYQEAMAAPVTAELHAPRSRVDARYAGEMVRRTMTERYGAEEAYTGGYTVYTTIEKD
CHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHH
RQEAANRALRSGLRDYDQRHGYRGPEGSMDAQAVGDPAAMNEVLGEHPPFGGLQAAVVLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCCCCCEEEEEE
VEPEAAWVWPRGGRVLQVDFETMEWARAYRGANRRGPAPEEAGDVLEVGDIVRVMPQGEG
ECCCCEEEECCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCC
RAALSAVPDVSGALVAQDPLDGRVRALAGGFDFRLSSFNRAVQARRQTGSVFKPFIYSAA
CCHHHCCCCCCCCEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LEGGYTPATLVNDAPVVFRDDALESYWRPGNYSGRFYGPTRLREALVFSRNLVSIRVLRD
HCCCCCCHHEECCCCEEEECHHHHHHCCCCCCCCCEECHHHHHHHHHHHCCHHEEEEHHH
VGIDPTIAHAVRMGFPPESLPRDLSLALGSNSATPLQMSNAFAAFANGGYRVFPNIIERV
CCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEECCCHHHHHHCCCEEEHHHHHHHH
EDGAGEVVFEPEFPEACELCRDDLPMASVPGDRTGLFDLPPPAERVLPEDNAWLISDMLA
HCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHH
DVIERGTGRGVRRVLDRGDLAGKTGTTNDLRDAWFVGYNQDLVATAWIGFDDQRSLGRGE
HHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCEEEEEEECCCCCCCCCCCC
TGASAALPIWARFMSVALDGVEERPMPRPGGLVTVRIDPESGRATSSENPDAIFETFREE
CCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHC
TVPVRDDSDRRQRGDEHTERELF
CCCCCCCCHHHHCCCCCHHHCCC
>Mature Secondary Structure
MKRFLRILLYSTLTLVGSGAVLGAAAVAYALLVLAPQLPDAESLRDVRFQQPLRVVSAEG
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHCCCCC
ELIAEYGEQRRQPEDIDDIPTDLLLAFIAAEDDRFYEHPGVDYQGLARAVSHVARERSFG
HHHHHHHHHCCCCCCCHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
PGGSTITMQVARNFFLSREVTILRKVNEILLALQIERTLSKEEILELYLNKIYLGNRAYG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCHH
VAAAAQVYYGKDLDELDLAQMAMIAGLPKAPSAANPIRNPRLALQRRAYVLGRMLNEGFI
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
SPERYQEAMAAPVTAELHAPRSRVDARYAGEMVRRTMTERYGAEEAYTGGYTVYTTIEKD
CHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHH
RQEAANRALRSGLRDYDQRHGYRGPEGSMDAQAVGDPAAMNEVLGEHPPFGGLQAAVVLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCCCCCEEEEEE
VEPEAAWVWPRGGRVLQVDFETMEWARAYRGANRRGPAPEEAGDVLEVGDIVRVMPQGEG
ECCCCEEEECCCCEEEEEEHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCC
RAALSAVPDVSGALVAQDPLDGRVRALAGGFDFRLSSFNRAVQARRQTGSVFKPFIYSAA
CCHHHCCCCCCCCEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LEGGYTPATLVNDAPVVFRDDALESYWRPGNYSGRFYGPTRLREALVFSRNLVSIRVLRD
HCCCCCCHHEECCCCEEEECHHHHHHCCCCCCCCCEECHHHHHHHHHHHCCHHEEEEHHH
VGIDPTIAHAVRMGFPPESLPRDLSLALGSNSATPLQMSNAFAAFANGGYRVFPNIIERV
CCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEECCCHHHHHHCCCEEEHHHHHHHH
EDGAGEVVFEPEFPEACELCRDDLPMASVPGDRTGLFDLPPPAERVLPEDNAWLISDMLA
HCCCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHH
DVIERGTGRGVRRVLDRGDLAGKTGTTNDLRDAWFVGYNQDLVATAWIGFDDQRSLGRGE
HHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCEEEEEEECCCCCCCCCCCC
TGASAALPIWARFMSVALDGVEERPMPRPGGLVTVRIDPESGRATSSENPDAIFETFREE
CCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHC
TVPVRDDSDRRQRGDEHTERELF
CCCCCCCCHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 7901733 [H]