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The map label for this gene is nadE [H]

Identifier: 121999017

GI number: 121999017

Start: 2452557

End: 2454173

Strand: Reverse

Name: nadE [H]

Synonym: Hhal_2238

Alternate gene names: 121999017

Gene position: 2454173-2452557 (Counterclockwise)

Preceding gene: 121999018

Following gene: 121999016

Centisome position: 91.63

GC content: 71.68

Gene sequence:

>1617_bases
ATGCGCATCGTCGTCGCTCAGCTGCCCTTTCCCGTCGGTGCCTTGGATGACAACGCCCGGCGGATCATCGATGCCATCGG
CCGGGCGCGGGACGAGTTCGGGGCCGACTTGGTGGTCTTCCCCGAGCTGGCCGTCACCGGTTATCCGCCCGACGATCTGC
TGCTGCGCAGCGACTTCCACGCCGCGGTGGAGCGGGCGCTGGCCCGGATCGCTGCGGCGTGCACGGGCGTCACCGCCCTG
GTCGGGGCCCCGCTGCACCGCCACGGCGTGGTGCGCAATGCCGCCGTGGCCCTGGCCGACGGCGCCGAGGTGGGGGCGGC
TTACAAGCGCTGCCTGCCGAGTTACAGCGTGTTCGACGACAACCGCCACTTCCGTGCCGGCGATGCCCCCTGCCTGCTCG
AGGTCGCCGGCCGGCGCATCGGGGTGGCCATCTGCGAGGACATCTGGGCGGCGACGCCGGCCGCCGAGGCTGCAGCGGCC
GGGGCCGAGCTGCTGGTCAGCATCAACGCCTCCCCGTTCCACGACGGCAAGCAGGCCGAGCGTGAGGGGGTCGCGGCTCG
GCGGGTGGCCGAGACCGGGGTTCCGCTGCTCTACGCCAACCTCGTCGGTGGTCACGACGAGGTGGTCTACGACGGCGGCT
CGTTCGCGCTGGACGATACCGGTGCGCTGAGCGCACGCGCCCCGGCCTTCGCCGACGGGCTGTATGCGCTGGACTGGCAA
GGGGGTGCGCTGCACGGCGAGCAGGCCGAGGCCGTCGAAGGGCCGCCGGCGGTCTACCAGGCGCTGGTGCGGGCCACCGC
CGACTACGTCCGCGGCAACGGCTTCTCCGGCGTTGTGTTGGGGCTCTCCGGGGGGATCGACTCGGCGCTGGTGGCCGCGG
TGGCCGCCGACGCGCTGGGCGGGGATCGGGTGCTGGCGGTCATGATGCCCACCCGCTACACCGCCGAGCGCAGCCTGGAG
GACGCCCGGGCGGCGGCCCAGGCCCTGGGGCTGGAGTATCGGAGCATCCCCATCGAGTCGCTCTTCGCCGACTACGAGCA
GGCCCTGGCCCCCCTCTTCGCCGGGCGGGAGGCGGATGCTACCGAGGAGAACATCCAGGCGCGCATCCGGGGCAACTGCC
TGATGGCGCTCTCCAACAAGCTGGGGTTGATGCTGCTGGCCGCCGGCAACAAGAGCGAGCTGGCGGTTGGTTACGCCACC
CTCTACGGCGATATGTGCGGGGGGTTCGCACCGCTCAAGGACGTCTACAAGACTGACGTCTACCGCCTGGCCGAGTACCG
CAATAGCCTGGGGCCTGCCGTGCCCGCCGCCATCATCGAGCGCGAGCCCACCGCGGAACTGCGCGCCGATCAGCGGGACA
GCGACAGCCTGCCGCCCTATCGGGTGCTTGATGCCGTGCTGCGCCAGTATCTGGAGGACGACGCCGCCGAGCACGAGATC
CGCGTCGAGGGGCTGGACGCGGTGGAGCTGTGCCGGGTGATCCGGCTGCTCTACCGGAACGAGTACAAGCGCCGCCAGGC
GGCCCCCGGGGTCAAGGTGACCCGGCGCGCCTTCGGGCGGGACCGCCGATATCCGATTACCTCGGGCTGGTCCGGTTGTC
AGGGCTGCAGCGACTAG

Upstream 100 bases:

>100_bases
ACAAGTTCGCCGCCCTGGAGGCGGCCGGGGTGGCCACCGTGCGCTCGCCGACCGAGATCGGCGAGCGCATCCGGCGGCTG
GTGGCGGGCTGAGGCAGACC

Downstream 100 bases:

>100_bases
CTCACGCTACCCTGAGGGTGGAGTCCAGAGCCCGAAACACTGAACCCAGAGCCATTGCGCCCCATCCCAAGGAGCTGCTC
GCATGAAGAAAGTCGAGGCG

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MRIVVAQLPFPVGALDDNARRIIDAIGRARDEFGADLVVFPELAVTGYPPDDLLLRSDFHAAVERALARIAAACTGVTAL
VGAPLHRHGVVRNAAVALADGAEVGAAYKRCLPSYSVFDDNRHFRAGDAPCLLEVAGRRIGVAICEDIWAATPAAEAAAA
GAELLVSINASPFHDGKQAEREGVAARRVAETGVPLLYANLVGGHDEVVYDGGSFALDDTGALSARAPAFADGLYALDWQ
GGALHGEQAEAVEGPPAVYQALVRATADYVRGNGFSGVVLGLSGGIDSALVAAVAADALGGDRVLAVMMPTRYTAERSLE
DARAAAQALGLEYRSIPIESLFADYEQALAPLFAGREADATEENIQARIRGNCLMALSNKLGLMLLAAGNKSELAVGYAT
LYGDMCGGFAPLKDVYKTDVYRLAEYRNSLGPAVPAAIIEREPTAELRADQRDSDSLPPYRVLDAVLRQYLEDDAAEHEI
RVEGLDAVELCRVIRLLYRNEYKRRQAAPGVKVTRRAFGRDRRYPITSGWSGCQGCSD

Sequences:

>Translated_538_residues
MRIVVAQLPFPVGALDDNARRIIDAIGRARDEFGADLVVFPELAVTGYPPDDLLLRSDFHAAVERALARIAAACTGVTAL
VGAPLHRHGVVRNAAVALADGAEVGAAYKRCLPSYSVFDDNRHFRAGDAPCLLEVAGRRIGVAICEDIWAATPAAEAAAA
GAELLVSINASPFHDGKQAEREGVAARRVAETGVPLLYANLVGGHDEVVYDGGSFALDDTGALSARAPAFADGLYALDWQ
GGALHGEQAEAVEGPPAVYQALVRATADYVRGNGFSGVVLGLSGGIDSALVAAVAADALGGDRVLAVMMPTRYTAERSLE
DARAAAQALGLEYRSIPIESLFADYEQALAPLFAGREADATEENIQARIRGNCLMALSNKLGLMLLAAGNKSELAVGYAT
LYGDMCGGFAPLKDVYKTDVYRLAEYRNSLGPAVPAAIIEREPTAELRADQRDSDSLPPYRVLDAVLRQYLEDDAAEHEI
RVEGLDAVELCRVIRLLYRNEYKRRQAAPGVKVTRRAFGRDRRYPITSGWSGCQGCSD
>Mature_538_residues
MRIVVAQLPFPVGALDDNARRIIDAIGRARDEFGADLVVFPELAVTGYPPDDLLLRSDFHAAVERALARIAAACTGVTAL
VGAPLHRHGVVRNAAVALADGAEVGAAYKRCLPSYSVFDDNRHFRAGDAPCLLEVAGRRIGVAICEDIWAATPAAEAAAA
GAELLVSINASPFHDGKQAEREGVAARRVAETGVPLLYANLVGGHDEVVYDGGSFALDDTGALSARAPAFADGLYALDWQ
GGALHGEQAEAVEGPPAVYQALVRATADYVRGNGFSGVVLGLSGGIDSALVAAVAADALGGDRVLAVMMPTRYTAERSLE
DARAAAQALGLEYRSIPIESLFADYEQALAPLFAGREADATEENIQARIRGNCLMALSNKLGLMLLAAGNKSELAVGYAT
LYGDMCGGFAPLKDVYKTDVYRLAEYRNSLGPAVPAAIIEREPTAELRADQRDSDSLPPYRVLDAVLRQYLEDDAAEHEI
RVEGLDAVELCRVIRLLYRNEYKRRQAAPGVKVTRRAFGRDRRYPITSGWSGCQGCSD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 57255; Mature: 57255

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVVAQLPFPVGALDDNARRIIDAIGRARDEFGADLVVFPELAVTGYPPDDLLLRSDFH
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCHHHEEHHHHH
AAVERALARIAAACTGVTALVGAPLHRHGVVRNAAVALADGAEVGAAYKRCLPSYSVFDD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHEEEECCHHHHHHHHHHCCCCCEECC
NRHFRAGDAPCLLEVAGRRIGVAICEDIWAATPAAEAAAAGAELLVSINASPFHDGKQAE
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCHHHH
REGVAARRVAETGVPLLYANLVGGHDEVVYDGGSFALDDTGALSARAPAFADGLYALDWQ
HHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEECCCCCCCCCCCHHHCCCEEEECC
GGALHGEQAEAVEGPPAVYQALVRATADYVRGNGFSGVVLGLSGGIDSALVAAVAADALG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
GDRVLAVMMPTRYTAERSLEDARAAAQALGLEYRSIPIESLFADYEQALAPLFAGREADA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
TEENIQARIRGNCLMALSNKLGLMLLAAGNKSELAVGYATLYGDMCGGFAPLKDVYKTDV
CHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHH
YRLAEYRNSLGPAVPAAIIEREPTAELRADQRDSDSLPPYRVLDAVLRQYLEDDAAEHEI
HHHHHHHHHCCCHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
RVEGLDAVELCRVIRLLYRNEYKRRQAAPGVKVTRRAFGRDRRYPITSGWSGCQGCSD
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIVVAQLPFPVGALDDNARRIIDAIGRARDEFGADLVVFPELAVTGYPPDDLLLRSDFH
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCEEECCCCCHHHEEHHHHH
AAVERALARIAAACTGVTALVGAPLHRHGVVRNAAVALADGAEVGAAYKRCLPSYSVFDD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHEEEECCHHHHHHHHHHCCCCCEECC
NRHFRAGDAPCLLEVAGRRIGVAICEDIWAATPAAEAAAAGAELLVSINASPFHDGKQAE
CCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCEEEEEECCCCCCCCHHHH
REGVAARRVAETGVPLLYANLVGGHDEVVYDGGSFALDDTGALSARAPAFADGLYALDWQ
HHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEECCCCCCCCCCCHHHCCCEEEECC
GGALHGEQAEAVEGPPAVYQALVRATADYVRGNGFSGVVLGLSGGIDSALVAAVAADALG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
GDRVLAVMMPTRYTAERSLEDARAAAQALGLEYRSIPIESLFADYEQALAPLFAGREADA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
TEENIQARIRGNCLMALSNKLGLMLLAAGNKSELAVGYATLYGDMCGGFAPLKDVYKTDV
CHHHHHHHHCCCEEEEECCCCCEEEEECCCCCCEEEHHHHHHHHHHCCHHHHHHHHHHHH
YRLAEYRNSLGPAVPAAIIEREPTAELRADQRDSDSLPPYRVLDAVLRQYLEDDAAEHEI
HHHHHHHHHCCCHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
RVEGLDAVELCRVIRLLYRNEYKRRQAAPGVKVTRRAFGRDRRYPITSGWSGCQGCSD
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]