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The map label for this gene is pleD [H]

Identifier: 121999012

GI number: 121999012

Start: 2448316

End: 2449602

Strand: Reverse

Name: pleD [H]

Synonym: Hhal_2233

Alternate gene names: 121999012

Gene position: 2449602-2448316 (Counterclockwise)

Preceding gene: 121999013

Following gene: 121999011

Centisome position: 91.46

GC content: 68.92

Gene sequence:

>1287_bases
ATGGTCGAGGAGCAGCGCGTCGCCGATTGCGTCCTGGTGGTGGAGGATAGCCGTGCCGTGGCCGGCTGGCTGGCCGGGCG
CATCGAGGACGCCCTGGGCCTGCGGGCGCTGGTCGCCGGCAGCCGCGCAGCGGCTCGGGAGTGGCTGCACACCCATGCGG
AGCGCATCAGTGCCGCGGTGCTCGATCTGGATCTGCCGGATGCGCCCCACGGCGAGGTGGCCGGGGACCTGGTGGAGCGG
CAGATCCCGTCGGTGGTCCTGACCGCCACCATCGAAGACGACCTGCGCGACCAGCTCGTCGCCGCCGGCGTCTGCGATTA
CGTCCTCAAGGACGCCCCGGATGCCATTGACTCCGTGCTGCGCACCCTGCATCGCATCCATCGCAACCACGACATCGGGG
TGCTGGTGGTTGACGACTCCCGCTCGGCGCGGGCCTATCTGGTCCACCTCCTCGGCCGCTGGGGGTTCTGTTGCTACGAG
GCCGGCGACGGCGAGCAGGCGCTGCAGCAGATCGACGCCGAGACCGACACCCCCATCCGGCTGGTCCTCTGCGACCAGAA
CATGCCGGGGATGGACGGCATCACCCTGATCCGGGAGCTGCGCCGTCGACACACCACGCAACGGCTAGGGATCATCGGGG
TCTCCAGTCACGGCTCCGGGCTGCTCTCCGCGCGGCTGCTCAAGGCCGGGGCCGATGACTTCCTGACCCGCCCTTTCCTC
GAAGAGGAGCTCAGTGTCCGCGTCAATCAGAACGTGGACCTCATGGAACTGCTGCGCGAGGCCCGGGAAGGGGCCCGGCG
GGATCCGCTGACCGGGTTGCACAACCGGCTCTACCTCGACGAGATCGCTGAGCAGCTGGGCGAGACGGCGCGGCGCACCC
AGCAGCCGCTGGGGGCGCTGGTGGTGGACCTCGACCACTTCAAGTCGATCAACGACCGCCTGGGCCACTTCGGCGGTGAC
ACGGTGCTCAAGCGGGTCGCTGACCAGCTGCGCGATACCGTCCGGCGTGCCGACGTGCTGGTCCGTTCTGGGGGCGAGGA
GTTCTACATCCTCACCCTGGGGATCGATACGGCCGGTGCCCGAACCCTAGGCGAACGGATTCGCAACGGCATCGAGACGC
TGGAGCTGGTCTATGAGGGCGAGACCATCACGACCAGTGCCAGCGTCGGGGTGGCCGTGCTCGAGGGCGGCTCGGTGGAG
GAGGCTCTGAAAGCCGCCGATCTGGCCATGTACGAGGCCAAGCGCCAGGGGCGCAACCGCGTCGCGGTCCTGCCTGAGAC
GGATTGA

Upstream 100 bases:

>100_bases
GCTGGTGCACGTACACGGATCGGGCGCGCTTCTTCTCCCATCGCCGGGATGGACCGACCGGCCGGATGGCAAGCCTGGTC
TACCGAGAGGAGCCGTGAGC

Downstream 100 bases:

>100_bases
AAACCCATCCTCGCCGCCCCATCTATCCTGACAAGGGTCAAGCCATCGCCAACGTGCGGTGAACCGGATCGTCGGCCCGA
CATGCGGCCAAGGGGGTAGA

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MVEEQRVADCVLVVEDSRAVAGWLAGRIEDALGLRALVAGSRAAAREWLHTHAERISAAVLDLDLPDAPHGEVAGDLVER
QIPSVVLTATIEDDLRDQLVAAGVCDYVLKDAPDAIDSVLRTLHRIHRNHDIGVLVVDDSRSARAYLVHLLGRWGFCCYE
AGDGEQALQQIDAETDTPIRLVLCDQNMPGMDGITLIRELRRRHTTQRLGIIGVSSHGSGLLSARLLKAGADDFLTRPFL
EEELSVRVNQNVDLMELLREAREGARRDPLTGLHNRLYLDEIAEQLGETARRTQQPLGALVVDLDHFKSINDRLGHFGGD
TVLKRVADQLRDTVRRADVLVRSGGEEFYILTLGIDTAGARTLGERIRNGIETLELVYEGETITTSASVGVAVLEGGSVE
EALKAADLAMYEAKRQGRNRVAVLPETD

Sequences:

>Translated_428_residues
MVEEQRVADCVLVVEDSRAVAGWLAGRIEDALGLRALVAGSRAAAREWLHTHAERISAAVLDLDLPDAPHGEVAGDLVER
QIPSVVLTATIEDDLRDQLVAAGVCDYVLKDAPDAIDSVLRTLHRIHRNHDIGVLVVDDSRSARAYLVHLLGRWGFCCYE
AGDGEQALQQIDAETDTPIRLVLCDQNMPGMDGITLIRELRRRHTTQRLGIIGVSSHGSGLLSARLLKAGADDFLTRPFL
EEELSVRVNQNVDLMELLREAREGARRDPLTGLHNRLYLDEIAEQLGETARRTQQPLGALVVDLDHFKSINDRLGHFGGD
TVLKRVADQLRDTVRRADVLVRSGGEEFYILTLGIDTAGARTLGERIRNGIETLELVYEGETITTSASVGVAVLEGGSVE
EALKAADLAMYEAKRQGRNRVAVLPETD
>Mature_428_residues
MVEEQRVADCVLVVEDSRAVAGWLAGRIEDALGLRALVAGSRAAAREWLHTHAERISAAVLDLDLPDAPHGEVAGDLVER
QIPSVVLTATIEDDLRDQLVAAGVCDYVLKDAPDAIDSVLRTLHRIHRNHDIGVLVVDDSRSARAYLVHLLGRWGFCCYE
AGDGEQALQQIDAETDTPIRLVLCDQNMPGMDGITLIRELRRRHTTQRLGIIGVSSHGSGLLSARLLKAGADDFLTRPFL
EEELSVRVNQNVDLMELLREAREGARRDPLTGLHNRLYLDEIAEQLGETARRTQQPLGALVVDLDHFKSINDRLGHFGGD
TVLKRVADQLRDTVRRADVLVRSGGEEFYILTLGIDTAGARTLGERIRNGIETLELVYEGETITTSASVGVAVLEGGSVE
EALKAADLAMYEAKRQGRNRVAVLPETD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=190, Percent_Identity=36.8421052631579, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI145693134, Length=176, Percent_Identity=42.0454545454545, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=38.150289017341, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=41.6149068322981, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=33.5164835164835, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081977, Length=181, Percent_Identity=34.8066298342541, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081881, Length=160, Percent_Identity=36.875, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787802, Length=175, Percent_Identity=28, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=168, Percent_Identity=32.7380952380952, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=33.7278106508876, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI87081974, Length=177, Percent_Identity=29.3785310734463, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=31.8965517241379, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790863, Length=114, Percent_Identity=35.0877192982456, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693157, Length=125, Percent_Identity=34.4, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46926; Mature: 46926

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEEQRVADCVLVVEDSRAVAGWLAGRIEDALGLRALVAGSRAAAREWLHTHAERISAAV
CCCHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LDLDLPDAPHGEVAGDLVERQIPSVVLTATIEDDLRDQLVAAGVCDYVLKDAPDAIDSVL
EECCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RTLHRIHRNHDIGVLVVDDSRSARAYLVHLLGRWGFCCYEAGDGEQALQQIDAETDTPIR
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCEE
LVLCDQNMPGMDGITLIRELRRRHTTQRLGIIGVSSHGSGLLSARLLKAGADDFLTRPFL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCHHHHHCCHH
EEELSVRVNQNVDLMELLREAREGARRDPLTGLHNRLYLDEIAEQLGETARRTQQPLGAL
HHHHHEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHE
VVDLDHFKSINDRLGHFGGDTVLKRVADQLRDTVRRADVLVRSGGEEFYILTLGIDTAGA
EEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCH
RTLGERIRNGIETLELVYEGETITTSASVGVAVLEGGSVEEALKAADLAMYEAKRQGRNR
HHHHHHHHHHHHHHHHEECCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCE
VAVLPETD
EEEECCCC
>Mature Secondary Structure
MVEEQRVADCVLVVEDSRAVAGWLAGRIEDALGLRALVAGSRAAAREWLHTHAERISAAV
CCCHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LDLDLPDAPHGEVAGDLVERQIPSVVLTATIEDDLRDQLVAAGVCDYVLKDAPDAIDSVL
EECCCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RTLHRIHRNHDIGVLVVDDSRSARAYLVHLLGRWGFCCYEAGDGEQALQQIDAETDTPIR
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCEE
LVLCDQNMPGMDGITLIRELRRRHTTQRLGIIGVSSHGSGLLSARLLKAGADDFLTRPFL
EEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCHHHHHCCHH
EEELSVRVNQNVDLMELLREAREGARRDPLTGLHNRLYLDEIAEQLGETARRTQQPLGAL
HHHHHEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHE
VVDLDHFKSINDRLGHFGGDTVLKRVADQLRDTVRRADVLVRSGGEEFYILTLGIDTAGA
EEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCH
RTLGERIRNGIETLELVYEGETITTSASVGVAVLEGGSVEEALKAADLAMYEAKRQGRNR
HHHHHHHHHHHHHHHHEECCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCE
VAVLPETD
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA