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The map label for this gene is pleD [H]

Identifier: 121998936

GI number: 121998936

Start: 2369082

End: 2370815

Strand: Reverse

Name: pleD [H]

Synonym: Hhal_2157

Alternate gene names: 121998936

Gene position: 2370815-2369082 (Counterclockwise)

Preceding gene: 121998937

Following gene: 121998935

Centisome position: 88.51

GC content: 67.01

Gene sequence:

>1734_bases
GTGGATAGAGTGGCCGACCAATCACTGCCTACCTTCCTGGTCGTCGATGATGAGCCGGTGAACACCATGCTCCTCGAGGC
GATCCTCGGGGCGCGGGGCTTTCACGTGATCACGGCGACCAATGGCGAGGAGGCGGTGGCCCGGTTCCGCGAGCAGCCGG
TGGACATGATCCTCATGGACATCATGATGCCGCGGATGGACGGCTATCAGGCCACCCGGCAGATCAAGGCCATGGACACC
GAGGGGTTCATCCCGGTGCTCTTCGTCACCGCGCTCACCGACGAGCAGCGGCTGGCACAGTGCGTCGAGGCCGGCGGCGA
CGACTTCGTCACCAAGCCCATCAGCCGGGTCCAGCTCAACGCCAAGATCGACTCCTGGCTGCGCACCCGCGGTCTCTACC
ACACCGTGGTGGCCCAGCGTGACGTCCTGCGTAGCCACCAGGAGCGGCTCGAGCGGGAGCAGGAGACCGCCGAGCGCATC
GTGGCCCGGGCCACGGAGTCCAGCGACCTGCGCGCCCCCGGAGTACGCTTCCATTATCAACCTGCGGCCATTCTCAGCGG
CGATATCCTCCTCGCTGCCCGCCGGCCCAACGGCAACCTGTTGATCCTCATCGGAGACTTCACCGGGCACGGGATCGGGG
CAGCGGTCGGTGTGCCCGGTCTGGCGGCGGTTTTCTACGATCGGGTGGGGCGCGGCGCCCATCCCGCCGAATTGCTCGAC
GACGTCAACGAGAAGCTCTTCCGGAGCCTGCCGGCGGACATGTTCCTGACCGGGGCCCTCCTGGAGGTGGATTTCCACGG
GCATCGCCTGGGGGTCTGGAACGCCGGGATGCCTCCGGTGTGGCTGATCCGCGACGGCGGCGTGGTGCAGCGTTTCCACT
CGGTGGATCTGCCGTTGGGCATCATCCGCGACACGGCTCCGGGGCGGCGTCAGTTGGAGTATATGGCGCTGACGCCGGGG
CTGCACGCGTTTGCCTGTTCCGACGGTATCGTCGAGGCCACCGATGCGGATGGTCAGCTCTTCGGCAACGAACGGATCGA
GGATGTGCTCTGCAGTGGCGACCCGGTCGCCGCCTTCGAACCGCTGCTGGAAGCGGTCTGCGCCTGGCGCGGCGGCGACG
AGCAAGCCGACGATACCACGCTGGTGTCCCTGGATCTGGACCGGTTGCTGCTCGAGGGCGAGGTGCGTGGGTATGGCGCT
GCCGCCTCGGAGAACTGGTACAGCGAACTGGTGCTGGACGCGGCAACCTTGGCCCATGTTAATCCGGTGCCGCCGATCAT
GAATCTGCTCGCCGACCTTGGGGCGCTGGAGGAGGATCGGCAGAGCCTCTACGTGGTGATCTCCGAACTTTTTACCAACG
CCCTGGAGCACGGGGTGCTTAAGCTCGACTCCGCGCTCAAGGGGAGCCCGGACGGTTTCGAGGCCTACTACCAGGAGCGT
ACCCGGGCCCTGGAAGAGTTGCAGGAAGGCGAGATCCGCCTGCGGGTGCAGTGCGACGCCGAGGGGGGCGGTGGCCAGGG
CATTATGATCGAGGTGGAAGACTCCGGGGAGGGGTTCGACTTCCAGCCCGTCCTGGAAGGTGGGCGTGGTCGAGACGCGC
CCGTCGGCGCCGGGCGGGGTATCCTGCTGGTGCGTTCGCTCTGCGACGAGCTCACCTATTTCCCCCCCGGTAACCGCGTG
CGTGTGCGCTACACCCCGGGCGGTCGTCGTCGCCCCTCGCCGGCGGATTCGTAG

Upstream 100 bases:

>100_bases
GGTGCGTAAGATCCTCGAGATCGCCAACTTCCAGCGCCTGTTTACCATCGAGGAGTGACCCCCATGGGCCGCGCGGGCGT
TTCGGAGCAGAGCACACGTC

Downstream 100 bases:

>100_bases
TAGCATCCGGCGGCATGCGCTTGCGTCTACCTCAACCCCCTGTGCGCTGGTGGCCGGGCTATCGCCCCTTCCTCCTCGGG
TTAGTGCTGCTTATCGGGGT

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MDRVADQSLPTFLVVDDEPVNTMLLEAILGARGFHVITATNGEEAVARFREQPVDMILMDIMMPRMDGYQATRQIKAMDT
EGFIPVLFVTALTDEQRLAQCVEAGGDDFVTKPISRVQLNAKIDSWLRTRGLYHTVVAQRDVLRSHQERLEREQETAERI
VARATESSDLRAPGVRFHYQPAAILSGDILLAARRPNGNLLILIGDFTGHGIGAAVGVPGLAAVFYDRVGRGAHPAELLD
DVNEKLFRSLPADMFLTGALLEVDFHGHRLGVWNAGMPPVWLIRDGGVVQRFHSVDLPLGIIRDTAPGRRQLEYMALTPG
LHAFACSDGIVEATDADGQLFGNERIEDVLCSGDPVAAFEPLLEAVCAWRGGDEQADDTTLVSLDLDRLLLEGEVRGYGA
AASENWYSELVLDAATLAHVNPVPPIMNLLADLGALEEDRQSLYVVISELFTNALEHGVLKLDSALKGSPDGFEAYYQER
TRALEELQEGEIRLRVQCDAEGGGGQGIMIEVEDSGEGFDFQPVLEGGRGRDAPVGAGRGILLVRSLCDELTYFPPGNRV
RVRYTPGGRRRPSPADS

Sequences:

>Translated_577_residues
MDRVADQSLPTFLVVDDEPVNTMLLEAILGARGFHVITATNGEEAVARFREQPVDMILMDIMMPRMDGYQATRQIKAMDT
EGFIPVLFVTALTDEQRLAQCVEAGGDDFVTKPISRVQLNAKIDSWLRTRGLYHTVVAQRDVLRSHQERLEREQETAERI
VARATESSDLRAPGVRFHYQPAAILSGDILLAARRPNGNLLILIGDFTGHGIGAAVGVPGLAAVFYDRVGRGAHPAELLD
DVNEKLFRSLPADMFLTGALLEVDFHGHRLGVWNAGMPPVWLIRDGGVVQRFHSVDLPLGIIRDTAPGRRQLEYMALTPG
LHAFACSDGIVEATDADGQLFGNERIEDVLCSGDPVAAFEPLLEAVCAWRGGDEQADDTTLVSLDLDRLLLEGEVRGYGA
AASENWYSELVLDAATLAHVNPVPPIMNLLADLGALEEDRQSLYVVISELFTNALEHGVLKLDSALKGSPDGFEAYYQER
TRALEELQEGEIRLRVQCDAEGGGGQGIMIEVEDSGEGFDFQPVLEGGRGRDAPVGAGRGILLVRSLCDELTYFPPGNRV
RVRYTPGGRRRPSPADS
>Mature_577_residues
MDRVADQSLPTFLVVDDEPVNTMLLEAILGARGFHVITATNGEEAVARFREQPVDMILMDIMMPRMDGYQATRQIKAMDT
EGFIPVLFVTALTDEQRLAQCVEAGGDDFVTKPISRVQLNAKIDSWLRTRGLYHTVVAQRDVLRSHQERLEREQETAERI
VARATESSDLRAPGVRFHYQPAAILSGDILLAARRPNGNLLILIGDFTGHGIGAAVGVPGLAAVFYDRVGRGAHPAELLD
DVNEKLFRSLPADMFLTGALLEVDFHGHRLGVWNAGMPPVWLIRDGGVVQRFHSVDLPLGIIRDTAPGRRQLEYMALTPG
LHAFACSDGIVEATDADGQLFGNERIEDVLCSGDPVAAFEPLLEAVCAWRGGDEQADDTTLVSLDLDRLLLEGEVRGYGA
AASENWYSELVLDAATLAHVNPVPPIMNLLADLGALEEDRQSLYVVISELFTNALEHGVLKLDSALKGSPDGFEAYYQER
TRALEELQEGEIRLRVQCDAEGGGGQGIMIEVEDSGEGFDFQPVLEGGRGRDAPVGAGRGILLVRSLCDELTYFPPGNRV
RVRYTPGGRRRPSPADS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=112, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1790437, Length=124, Percent_Identity=33.0645161290323, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789149, Length=128, Percent_Identity=34.375, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788394, Length=123, Percent_Identity=32.520325203252, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=255, Percent_Identity=24.7058823529412, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=34.7457627118644, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790863, Length=138, Percent_Identity=27.536231884058, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=114, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 63056; Mature: 63056

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVADQSLPTFLVVDDEPVNTMLLEAILGARGFHVITATNGEEAVARFREQPVDMILMD
CCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCHHHHHHH
IMMPRMDGYQATRQIKAMDTEGFIPVLFVTALTDEQRLAQCVEAGGDDFVTKPISRVQLN
HHHCCCCCHHHHHHHEECCCCCCCCEEHEECCCCHHHHHHHHHCCCCCHHHCCHHHEEEC
AKIDSWLRTRGLYHTVVAQRDVLRSHQERLEREQETAERIVARATESSDLRAPGVRFHYQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
PAAILSGDILLAARRPNGNLLILIGDFTGHGIGAAVGVPGLAAVFYDRVGRGAHPAELLD
CHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHHHH
DVNEKLFRSLPADMFLTGALLEVDFHGHRLGVWNAGMPPVWLIRDGGVVQRFHSVDLPLG
HHHHHHHHHCCHHHHHCCEEEEEEECCCEEEEECCCCCCEEEEECCCHHHHHHHCCCCCE
IIRDTAPGRRQLEYMALTPGLHAFACSDGIVEATDADGQLFGNERIEDVLCSGDPVAAFE
EEECCCCCHHEEEEEEECCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHCCCCCHHHHH
PLLEAVCAWRGGDEQADDTTLVSLDLDRLLLEGEVRGYGAAASENWYSELVLDAATLAHV
HHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
NPVPPIMNLLADLGALEEDRQSLYVVISELFTNALEHGVLKLDSALKGSPDGFEAYYQER
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEECHHHCCCCCHHHHHHHHH
TRALEELQEGEIRLRVQCDAEGGGGQGIMIEVEDSGEGFDFQPVLEGGRGRDAPVGAGRG
HHHHHHHCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCH
ILLVRSLCDELTYFPPGNRVRVRYTPGGRRRPSPADS
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MDRVADQSLPTFLVVDDEPVNTMLLEAILGARGFHVITATNGEEAVARFREQPVDMILMD
CCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCHHHHHHH
IMMPRMDGYQATRQIKAMDTEGFIPVLFVTALTDEQRLAQCVEAGGDDFVTKPISRVQLN
HHHCCCCCHHHHHHHEECCCCCCCCEEHEECCCCHHHHHHHHHCCCCCHHHCCHHHEEEC
AKIDSWLRTRGLYHTVVAQRDVLRSHQERLEREQETAERIVARATESSDLRAPGVRFHYQ
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEC
PAAILSGDILLAARRPNGNLLILIGDFTGHGIGAAVGVPGLAAVFYDRVGRGAHPAELLD
CHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHHHH
DVNEKLFRSLPADMFLTGALLEVDFHGHRLGVWNAGMPPVWLIRDGGVVQRFHSVDLPLG
HHHHHHHHHCCHHHHHCCEEEEEEECCCEEEEECCCCCCEEEEECCCHHHHHHHCCCCCE
IIRDTAPGRRQLEYMALTPGLHAFACSDGIVEATDADGQLFGNERIEDVLCSGDPVAAFE
EEECCCCCHHEEEEEEECCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHCCCCCHHHHH
PLLEAVCAWRGGDEQADDTTLVSLDLDRLLLEGEVRGYGAAASENWYSELVLDAATLAHV
HHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCC
NPVPPIMNLLADLGALEEDRQSLYVVISELFTNALEHGVLKLDSALKGSPDGFEAYYQER
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEECHHHCCCCCHHHHHHHHH
TRALEELQEGEIRLRVQCDAEGGGGQGIMIEVEDSGEGFDFQPVLEGGRGRDAPVGAGRG
HHHHHHHCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCH
ILLVRSLCDELTYFPPGNRVRVRYTPGGRRRPSPADS
HHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA