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The map label for this gene is pmbA [H]

Identifier: 121998912

GI number: 121998912

Start: 2331744

End: 2333090

Strand: Reverse

Name: pmbA [H]

Synonym: Hhal_2133

Alternate gene names: 121998912

Gene position: 2333090-2331744 (Counterclockwise)

Preceding gene: 121998913

Following gene: 121998899

Centisome position: 87.11

GC content: 71.42

Gene sequence:

>1347_bases
ATGCCGGATCCACGCAACGAGCTACCTGCCCCGGGAACGCTGGAGGACCTGGTGCAGCAGGCCCTCGACGAGGCCCGCCA
GCACGGCGCCGACGCAGCGGAGGCGGCCTGCTCCGGGGATGTCGGTCTGTCGGTGAATGTGCGCAAGGGCGAGATCGATA
CCCTGGAGCACCACCGCGGACGGGACATGGGGGTTACGGTCTACTTCGGCGGCCGCAAGGGCAGTGCCTCGGCCGGCGAA
CTCTCCGAGCAGGCGGTGCGCGACGCCGTTCAGGCGGCCTGCGACATCGCCCGCTATACCTCCGAGGATCCGGCCAATGG
GCTGGCGCCGGCCGAGCGCATGGCCGTCCATCCGCCGGACCTGGACCTCGACCACCCCTGGCCGCTGGCGGCGGATGACG
CGGTCGAGCTGGCCCGTGACTGCGAGTCTGCGGCGGTCGAGGCCGACCCGCGCATCGAGAATACCGAGGGGGCCGGGGTG
AGCATCCACCGAGGCCTGCACGCCTACGGCAACAGCCACGGCTTCTTCTCGGTGCTCCCGGAGTCGCGGCACAGCATCCA
CTGCGTGGCGGTGGCCGGCCGCGGCGAAGCCATGCAGCGAGACTATTGGTACACCGTGGCCCGCGATCCGGCGGCCCTGG
AGGACGCCCGGAGCGTGGGGCGGCAGGCGGCCGAGCACGCGGTCGCGCGGCTCGGTGCCGAGAGTCTGAGCACCGAGCGG
GTGCCGGTGCTGTTCCGTGCCCCGGTGGCCCGCGGGCTGGTCGGCCACTTGGTCTCGGCGGTGCGCGGCAGCGCGCTGTA
CCGGCAGGCGTCGTTCCTGGTGGACAGCGCCGGCGAGCAGCTCTTCCCGGCGTGGCTGCAGATGGTCGAGCGGCCGCATA
TCCGCCAGGCCCTGGGGAGTACCGCCTTCGATGCCGAGGGGGTCGCCACTGCCGATTCGGCGCTGGTCCAGGACGGGGTG
CTGCAGCGCTACGTGCTCGACAGCTACTCGGCGCGGCGCCTGGGGCTGGAGACCACGGCCAATGCCGGCGGGGTCCACAA
CCTGGAGGTTTCGGGGGGCGCCGGGGAGGACTTCGAAGGGCTGATCCGGCGCATGGGCCGGGGGCTGGTGGTCACCGAAC
TCATGGGCCAGGGGGTCAACCTGGTGACCGGTGACTACTCCCGCGGTGCCGCCGGCTTCTGGGTGGAGAACGGGGTCATC
GACCGTCCCGTGCAGGAGATCACCATCGCCGGCCATCTGCGCGATCTGTTCGCCGGGATCCAGGTGGTGGGCAGCGACGT
GGACCGCCGTGGGAACATCCGTTGTGGCTCCATCCTGGTCGAGGAGATGACCGTCGCCGGGCAGTAA

Upstream 100 bases:

>100_bases
CCGACGCTCAAGGTCGACGGCATGACCGTCGGTGGCACGCAGGCCTAGCCGCACCGCGCCGCTGCCTGGAAACCCAACTC
ACAACGGAAAAGGCGAACCC

Downstream 100 bases:

>100_bases
GCGCCCGGCGGCGATCAGCCGTGCTCGGTCTCGCCGAATCCCTCGGTGACCAGCCGGACCAGGGCCTCGAGGGCTGGCTC
GGCGTCCGTCCCCTCGGCCT

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MPDPRNELPAPGTLEDLVQQALDEARQHGADAAEAACSGDVGLSVNVRKGEIDTLEHHRGRDMGVTVYFGGRKGSASAGE
LSEQAVRDAVQAACDIARYTSEDPANGLAPAERMAVHPPDLDLDHPWPLAADDAVELARDCESAAVEADPRIENTEGAGV
SIHRGLHAYGNSHGFFSVLPESRHSIHCVAVAGRGEAMQRDYWYTVARDPAALEDARSVGRQAAEHAVARLGAESLSTER
VPVLFRAPVARGLVGHLVSAVRGSALYRQASFLVDSAGEQLFPAWLQMVERPHIRQALGSTAFDAEGVATADSALVQDGV
LQRYVLDSYSARRLGLETTANAGGVHNLEVSGGAGEDFEGLIRRMGRGLVVTELMGQGVNLVTGDYSRGAAGFWVENGVI
DRPVQEITIAGHLRDLFAGIQVVGSDVDRRGNIRCGSILVEEMTVAGQ

Sequences:

>Translated_448_residues
MPDPRNELPAPGTLEDLVQQALDEARQHGADAAEAACSGDVGLSVNVRKGEIDTLEHHRGRDMGVTVYFGGRKGSASAGE
LSEQAVRDAVQAACDIARYTSEDPANGLAPAERMAVHPPDLDLDHPWPLAADDAVELARDCESAAVEADPRIENTEGAGV
SIHRGLHAYGNSHGFFSVLPESRHSIHCVAVAGRGEAMQRDYWYTVARDPAALEDARSVGRQAAEHAVARLGAESLSTER
VPVLFRAPVARGLVGHLVSAVRGSALYRQASFLVDSAGEQLFPAWLQMVERPHIRQALGSTAFDAEGVATADSALVQDGV
LQRYVLDSYSARRLGLETTANAGGVHNLEVSGGAGEDFEGLIRRMGRGLVVTELMGQGVNLVTGDYSRGAAGFWVENGVI
DRPVQEITIAGHLRDLFAGIQVVGSDVDRRGNIRCGSILVEEMTVAGQ
>Mature_447_residues
PDPRNELPAPGTLEDLVQQALDEARQHGADAAEAACSGDVGLSVNVRKGEIDTLEHHRGRDMGVTVYFGGRKGSASAGEL
SEQAVRDAVQAACDIARYTSEDPANGLAPAERMAVHPPDLDLDHPWPLAADDAVELARDCESAAVEADPRIENTEGAGVS
IHRGLHAYGNSHGFFSVLPESRHSIHCVAVAGRGEAMQRDYWYTVARDPAALEDARSVGRQAAEHAVARLGAESLSTERV
PVLFRAPVARGLVGHLVSAVRGSALYRQASFLVDSAGEQLFPAWLQMVERPHIRQALGSTAFDAEGVATADSALVQDGVL
QRYVLDSYSARRLGLETTANAGGVHNLEVSGGAGEDFEGLIRRMGRGLVVTELMGQGVNLVTGDYSRGAAGFWVENGVID
RPVQEITIAGHLRDLFAGIQVVGSDVDRRGNIRCGSILVEEMTVAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=435, Percent_Identity=49.1954022988506, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47617; Mature: 47485

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDPRNELPAPGTLEDLVQQALDEARQHGADAAEAACSGDVGLSVNVRKGEIDTLEHHRG
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHCC
RDMGVTVYFGGRKGSASAGELSEQAVRDAVQAACDIARYTSEDPANGLAPAERMAVHPPD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCC
LDLDHPWPLAADDAVELARDCESAAVEADPRIENTEGAGVSIHRGLHAYGNSHGFFSVLP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHCCHHHCCCCCCEEEECC
ESRHSIHCVAVAGRGEAMQRDYWYTVARDPAALEDARSVGRQAAEHAVARLGAESLSTER
CCCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCC
VPVLFRAPVARGLVGHLVSAVRGSALYRQASFLVDSAGEQLFPAWLQMVERPHIRQALGS
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCC
TAFDAEGVATADSALVQDGVLQRYVLDSYSARRLGLETTANAGGVHNLEVSGGAGEDFEG
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEECCCCCCCHHH
LIRRMGRGLVVTELMGQGVNLVTGDYSRGAAGFWVENGVIDRPVQEITIAGHLRDLFAGI
HHHHHCCCEEEHHHHCCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
QVVGSDVDRRGNIRCGSILVEEMTVAGQ
HHHCCCCCCCCCEEHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDPRNELPAPGTLEDLVQQALDEARQHGADAAEAACSGDVGLSVNVRKGEIDTLEHHRG
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHCC
RDMGVTVYFGGRKGSASAGELSEQAVRDAVQAACDIARYTSEDPANGLAPAERMAVHPPD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCC
LDLDHPWPLAADDAVELARDCESAAVEADPRIENTEGAGVSIHRGLHAYGNSHGFFSVLP
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHCCHHHCCCCCCEEEECC
ESRHSIHCVAVAGRGEAMQRDYWYTVARDPAALEDARSVGRQAAEHAVARLGAESLSTER
CCCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCC
VPVLFRAPVARGLVGHLVSAVRGSALYRQASFLVDSAGEQLFPAWLQMVERPHIRQALGS
CCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCC
TAFDAEGVATADSALVQDGVLQRYVLDSYSARRLGLETTANAGGVHNLEVSGGAGEDFEG
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEECCCCCCCHHH
LIRRMGRGLVVTELMGQGVNLVTGDYSRGAAGFWVENGVIDRPVQEITIAGHLRDLFAGI
HHHHHCCCEEEHHHHCCCCEEEECCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
QVVGSDVDRRGNIRCGSILVEEMTVAGQ
HHHCCCCCCCCCEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]