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The map label for this gene is rho

Identifier: 121998834

GI number: 121998834

Start: 2258120

End: 2259379

Strand: Reverse

Name: rho

Synonym: Hhal_2055

Alternate gene names: 121998834

Gene position: 2259379-2258120 (Counterclockwise)

Preceding gene: 121998835

Following gene: 121998833

Centisome position: 84.35

GC content: 64.68

Gene sequence:

>1260_bases
ATGAATCTCACCGAGCTCAAGAGAAAGCCAGCCACCGAACTCCTGGAGATCGCCCAGTCCATGGGCATCGAGGGCACTGC
CCGCTCCCGGAAGCAGGACATCATCTTCGCCATCCTCAAGGCTCATGCCAAGAACGGCGATTCCATCTACGGCGACGGCG
TCCTCGAGATCCTCCAGGACGGCTTCGGCTTCCTGCGCTCCGCGGATGCCTCGTACATGGCCGGGCCGGACGATATCTAC
GTCTCGCCCAGCCAGATCCGCCGGTTCGCACTGCGCACCGGCGACACCATCACCGGCAAGATCCGCCCCCCGAAGGACGG
CGAGCGCTACTTCGCCCTGCTCAAGGTCGACCAGATCAACTTCGAGCCGCCGGAGGCGGCCAAGAACAAGGTCCTCTTCG
AGAACCTCACTCCGCTGTTCACCCGCGATCGCATGCGCATGGAGCGGGGCAACGGCTCCACCGAGGACCTCACCGCCCGG
GTCATTGACCTGGTGGCGCCTATCGGCAAGGGCCAGCGCGGGCTGATCGTCTCGCCCCCGAAGGCCGGCAAGACGATGAT
GCTCCAGAACGTCGCCCAGAGCATCACCTACAACTACCCGGAGTGCTACCTCATCGTCCTGCTCATCGACGAGCGGCCCG
AGGAGGTCACTGAGTTCGCCCGCTCGGTGCTCAGCGCCGAGACGGTCTCCTCGACCTTCGACGAGCCGGCCTCGCGCCAC
GTCCAGGTCGCCGAGATGGTCATCGAGAAGGCCAAGCGCCTGGTCGAGCACAAGAAGGACGTGGTCATCCTGCTCGACTC
CGTCACCCGCCTGGCGCGCGCCTATAATACGGTGGTGCCGTCGTCCGGTAAGGTGCTCACCGGGGGCGTGGATGCCAACG
CCCTGCACCGGCCCAAGCGCTTCTTCGGTGCCGCGCGCAACGTCGAAGAGGGCGGCAGCCTCACCATCCTGGCCACCGCC
CTGGTCGAGACCGGCTCGCGCATGGACGAGGTGATCTACGAGGAGTTCAAGGGCACCGGCAACATGGAGCTGCACATGGA
CCGGAAGATCGCCGAGAAGCGCATCTACCCGGCCATCCACCTGAACCGCTCTGGGACCCGGCGCGAGGAACTCCTGATGA
CCCCCGAGGAGCTGCAGAAGACCTGGATCCTGCGCAAGCTCCTGCACAACATGGACGAGGTGGCCGCCATCGAGTTCCTC
CTCGACAAGCTCAAGGACACCAAGACCAACACCGAGTTCTTCGAGGCCATGAAACGCTGA

Upstream 100 bases:

>100_bases
TGCCGCAGCGCCGCCCCCGGCTTGAGTCCCGCGCCATCGCGCCCCTGCGGACGGCCGGCGGGCCGGCGCCCCCGTGACCC
TCCCCACGCCGAAGCGAACC

Downstream 100 bases:

>100_bases
TGAGCCATCCCCTCCGTCGTGCCCGGGCCGCCCTGATCGCCGCCGTGGCGACGGTGGCGGCCGGGCCGGCGATGGGCCAC
GGCTATCAGGGCGAGCGCGG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNLTELKRKPATELLEIAQSMGIEGTARSRKQDIIFAILKAHAKNGDSIYGDGVLEILQDGFGFLRSADASYMAGPDDIY
VSPSQIRRFALRTGDTITGKIRPPKDGERYFALLKVDQINFEPPEAAKNKVLFENLTPLFTRDRMRMERGNGSTEDLTAR
VIDLVAPIGKGQRGLIVSPPKAGKTMMLQNVAQSITYNYPECYLIVLLIDERPEEVTEFARSVLSAETVSSTFDEPASRH
VQVAEMVIEKAKRLVEHKKDVVILLDSVTRLARAYNTVVPSSGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTILATA
LVETGSRMDEVIYEEFKGTGNMELHMDRKIAEKRIYPAIHLNRSGTRREELLMTPEELQKTWILRKLLHNMDEVAAIEFL
LDKLKDTKTNTEFFEAMKR

Sequences:

>Translated_419_residues
MNLTELKRKPATELLEIAQSMGIEGTARSRKQDIIFAILKAHAKNGDSIYGDGVLEILQDGFGFLRSADASYMAGPDDIY
VSPSQIRRFALRTGDTITGKIRPPKDGERYFALLKVDQINFEPPEAAKNKVLFENLTPLFTRDRMRMERGNGSTEDLTAR
VIDLVAPIGKGQRGLIVSPPKAGKTMMLQNVAQSITYNYPECYLIVLLIDERPEEVTEFARSVLSAETVSSTFDEPASRH
VQVAEMVIEKAKRLVEHKKDVVILLDSVTRLARAYNTVVPSSGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTILATA
LVETGSRMDEVIYEEFKGTGNMELHMDRKIAEKRIYPAIHLNRSGTRREELLMTPEELQKTWILRKLLHNMDEVAAIEFL
LDKLKDTKTNTEFFEAMKR
>Mature_419_residues
MNLTELKRKPATELLEIAQSMGIEGTARSRKQDIIFAILKAHAKNGDSIYGDGVLEILQDGFGFLRSADASYMAGPDDIY
VSPSQIRRFALRTGDTITGKIRPPKDGERYFALLKVDQINFEPPEAAKNKVLFENLTPLFTRDRMRMERGNGSTEDLTAR
VIDLVAPIGKGQRGLIVSPPKAGKTMMLQNVAQSITYNYPECYLIVLLIDERPEEVTEFARSVLSAETVSSTFDEPASRH
VQVAEMVIEKAKRLVEHKKDVVILLDSVTRLARAYNTVVPSSGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTILATA
LVETGSRMDEVIYEEFKGTGNMELHMDRKIAEKRIYPAIHLNRSGTRREELLMTPEELQKTWILRKLLHNMDEVAAIEFL
LDKLKDTKTNTEFFEAMKR

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=78.7589498806683, Blast_Score=701, Evalue=0.0,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47016; Mature: 47016

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTELKRKPATELLEIAQSMGIEGTARSRKQDIIFAILKAHAKNGDSIYGDGVLEILQD
CCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
GFGFLRSADASYMAGPDDIYVSPSQIRRFALRTGDTITGKIRPPKDGERYFALLKVDQIN
HHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCC
FEPPEAAKNKVLFENLTPLFTRDRMRMERGNGSTEDLTARVIDLVAPIGKGQRGLIVSPP
CCCCHHHHCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCC
KAGKTMMLQNVAQSITYNYPECYLIVLLIDERPEEVTEFARSVLSAETVSSTFDEPASRH
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
VQVAEMVIEKAKRLVEHKKDVVILLDSVTRLARAYNTVVPSSGKVLTGGVDANALHRPKR
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHH
FFGAARNVEEGGSLTILATALVETGSRMDEVIYEEFKGTGNMELHMDRKIAEKRIYPAIH
HHHHHCCCCCCCCEEEHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCEEE
LNRSGTRREELLMTPEELQKTWILRKLLHNMDEVAAIEFLLDKLKDTKTNTEFFEAMKR
ECCCCCCHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MNLTELKRKPATELLEIAQSMGIEGTARSRKQDIIFAILKAHAKNGDSIYGDGVLEILQD
CCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
GFGFLRSADASYMAGPDDIYVSPSQIRRFALRTGDTITGKIRPPKDGERYFALLKVDQIN
HHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCC
FEPPEAAKNKVLFENLTPLFTRDRMRMERGNGSTEDLTARVIDLVAPIGKGQRGLIVSPP
CCCCHHHHCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCC
KAGKTMMLQNVAQSITYNYPECYLIVLLIDERPEEVTEFARSVLSAETVSSTFDEPASRH
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
VQVAEMVIEKAKRLVEHKKDVVILLDSVTRLARAYNTVVPSSGKVLTGGVDANALHRPKR
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHH
FFGAARNVEEGGSLTILATALVETGSRMDEVIYEEFKGTGNMELHMDRKIAEKRIYPAIH
HHHHHCCCCCCCCEEEHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCCEEE
LNRSGTRREELLMTPEELQKTWILRKLLHNMDEVAAIEFLLDKLKDTKTNTEFFEAMKR
ECCCCCCHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8051015 [H]