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The map label for this gene is mrpA [H]

Identifier: 121998814

GI number: 121998814

Start: 2236864

End: 2239182

Strand: Reverse

Name: mrpA [H]

Synonym: Hhal_2035

Alternate gene names: 121998814

Gene position: 2239182-2236864 (Counterclockwise)

Preceding gene: 121998817

Following gene: 121998813

Centisome position: 83.6

GC content: 66.8

Gene sequence:

>2319_bases
ATGCTTGCCGCAGTACTTTCCGGATTCCTACTCGCCGTCCTCGTCCCGTGGCTGCAGCCCGTCCTGGGCCGCCGCGCCGG
CTGGGCCTTCGCTGCGCTTCCCGCGGTGCTCACCCTCTATTTTGCCACCTTCCTGCCGCAGATTGTCGGCGGCGAGACGG
TGTGGATGGAGTACACCTGGGTCCCCGGGCTCGACGTCAATCTGTCCTTCTACGTGGACGGGCTGTCGTTGCTCTTCGCC
CTGCTGATCTCGGGGATCGGCACCTTCGTCCTGCTCTACGCCAGCGACTACCTGGACGGCGACGATCGCCAGCCGCGCTT
TTATGTCCACATCCTCGCCTTCATGGCGTCGATGCTCGGCCTGGTGCTCTCGGACAACCTCATCACCCTGTTCATCTTCT
GGGAGCTGACCAGCATCACCTCGTACATGCTGATCGGCTACGACAACGAGGATGCCAAGGCCCGGCGCTACGCGCTGCAG
GGGCTGCTGGTGACCGTGGGCGGCGGGCTGGCGCTGCTGGCCGGCTTCATCATGCTGGGGATCGTCGGCGACACCTACAC
GATCTCCGAGCTGCTGGAGCAGGGTGACGTCATCCGCGAGCACGAGTGGTACGTGCCGCTGACGCTGCTCATCCTCCTCG
GCGCCTTCACCAAGTCGGCCCAGGTGCCGTTCCACTTCTGGCTGCCGCGGGCCATGGCGGCGCCGACGCCGGTCTCCGCC
TACCTGCACTCGGCCACCATGGTGAAGGCCGGCGTCTTCCTGCTGGCCAAGCTGCACCCGGCGCTGGGCGATACCGCGCT
GTGGATGACCCTGCTACCGCTGGTCGGCGCCATCACCATGTTCACCGGCGCCTACTTCGCCGTGCGCAGCACCGGCATCA
AGGGGGTGCTGGCCAACTCCACGGTGATGGCCCTGGGCACGCTGACGATGCTGGCCGGGATCGGCACCGAGGGGGCGATG
ATCGCCTTCGGCGCCTTCCTGCTCGCTCACTCCCTCTACAAGGGGGCGCTGTTCCTGGTTGCCGGCGCCCTAGATCACGA
GACCGGCACCAAGGAGATCCTGCAGATGGGCGGTCTGCGCCACGCCATGCCCAAGACCGCGGTGCTGGCCGCGGTCTCGG
CGCTGTCGCTGGCCGGTATCCCGCCGCTGTTCGGCTTCCTGGGCAAGGAGCTGATGCTCGAGTCGGTGCTCGAGGCGCCC
TACCTGTCGGCGGTGCTGGCCCTGCTGGCCCTGGGTACGGCGGTACTGGTGGTCATTGTCGCCGGTATCGTCGCCATCCG
GCCGTTCTTCGGCGAGCGCACCGAGACCCCGAAGGAGCCCCACGAGGCTCCGCCGGGGATGCTCGCCGGCCCGGCGGTCC
TTGGCACCCTGAGCGTGCTCTTTGGCCTGTTTCCGTTCCTGCCCGAGGGGCTGATCGGGGCGGTGGCCTCGTCGCTGGCC
GGTGAGCCGGTGTCGGTGAGCCTGTCCCTGTGGCACGGGATCAACCTGCCGCTGATCATGTCGCTGTTCGCCATCGCCGC
CGGTGCCTTCCTGTATACCCGCTGGGACCAGGTGCGCGAGGCCATGGGCCGCTACGACTGGCTCTACGAGCGCGGCCCCG
AGGCCGGTTACTTCCGCGTTATGGATGCGCTGGTGTGGAGCGCCGACTGGCAGACCCGAGTGCTGCAGAACGGTTACCTC
AGGCACTACATCACCACCACGGTGCTGACCACCGTGCTGCTGGTTGGCTACACCCTGGCCACCCGCTACGACGGCGGCTT
CAGCTTCGGCCTGGAGGTCTACTTCTACGAGTGGATCATCGCCGGCGTGCTGATCGCCTCGGTGCTCTTCGCCTGCGTGA
CCCAGTCGCGCCTCGGGGCGGTGGCCTCGCTGGGTGTGATGGGTTTCACCGTCGCGCTGATCTTCATTCTGTTCAGCGCC
CCGGATCTGGGCATTACGCAGATCCTGGTGGAGACCCTGACGGTGATCCTGCTGGTCCTGGTGCTCTTCCGGCTGCCCGG
CTTCATGGACTTCTCGACGCCGCAACAGCGGGTACGGGATGGCGTGGTGGCAGGAGTGATGGGCGTGCTGGTGACCTCGC
TGCTGCTGGTCACCATCGACGTGCAGTTTGCCGAACCGATTTCCGCCTACCACCTGGATGAGAGTGTGCCGCTGGCCCAC
GGCCGCAACGTGGTCAACGTCATCCTGGTGGACTACCGGGCCCTCGATACCCTGGGCGAGATCTTCGTGGTCGCCCTGGC
CGCCATCGGCGTTTACGCCATGGTCAAGTACCGGGTGACCGGTGAGCGCGGCGCCGCCGAGAAGGAGGACACGCAATGA

Upstream 100 bases:

>100_bases
CTTTCTTGTTTTGCGCTGCGCCAACTTGTCAGCCCCCGGATCATCCGGTATCTTCCACTGCCGTTGTCTGCCGACGAGAC
CGTCAGGAGATCGGCCTCGC

Downstream 100 bases:

>100_bases
TCCAGCCCGGATCCCTCATCCTCTCGACCATGGCGCGGGTGCTGCTGCCGCTGCAGCTGCTGTTCTCCATCTTCATGCTG
CTGCGTGGCCACGACCTGCC

Product: putative monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 772; Mature: 772

Protein sequence:

>772_residues
MLAAVLSGFLLAVLVPWLQPVLGRRAGWAFAALPAVLTLYFATFLPQIVGGETVWMEYTWVPGLDVNLSFYVDGLSLLFA
LLISGIGTFVLLYASDYLDGDDRQPRFYVHILAFMASMLGLVLSDNLITLFIFWELTSITSYMLIGYDNEDAKARRYALQ
GLLVTVGGGLALLAGFIMLGIVGDTYTISELLEQGDVIREHEWYVPLTLLILLGAFTKSAQVPFHFWLPRAMAAPTPVSA
YLHSATMVKAGVFLLAKLHPALGDTALWMTLLPLVGAITMFTGAYFAVRSTGIKGVLANSTVMALGTLTMLAGIGTEGAM
IAFGAFLLAHSLYKGALFLVAGALDHETGTKEILQMGGLRHAMPKTAVLAAVSALSLAGIPPLFGFLGKELMLESVLEAP
YLSAVLALLALGTAVLVVIVAGIVAIRPFFGERTETPKEPHEAPPGMLAGPAVLGTLSVLFGLFPFLPEGLIGAVASSLA
GEPVSVSLSLWHGINLPLIMSLFAIAAGAFLYTRWDQVREAMGRYDWLYERGPEAGYFRVMDALVWSADWQTRVLQNGYL
RHYITTTVLTTVLLVGYTLATRYDGGFSFGLEVYFYEWIIAGVLIASVLFACVTQSRLGAVASLGVMGFTVALIFILFSA
PDLGITQILVETLTVILLVLVLFRLPGFMDFSTPQQRVRDGVVAGVMGVLVTSLLLVTIDVQFAEPISAYHLDESVPLAH
GRNVVNVILVDYRALDTLGEIFVVALAAIGVYAMVKYRVTGERGAAEKEDTQ

Sequences:

>Translated_772_residues
MLAAVLSGFLLAVLVPWLQPVLGRRAGWAFAALPAVLTLYFATFLPQIVGGETVWMEYTWVPGLDVNLSFYVDGLSLLFA
LLISGIGTFVLLYASDYLDGDDRQPRFYVHILAFMASMLGLVLSDNLITLFIFWELTSITSYMLIGYDNEDAKARRYALQ
GLLVTVGGGLALLAGFIMLGIVGDTYTISELLEQGDVIREHEWYVPLTLLILLGAFTKSAQVPFHFWLPRAMAAPTPVSA
YLHSATMVKAGVFLLAKLHPALGDTALWMTLLPLVGAITMFTGAYFAVRSTGIKGVLANSTVMALGTLTMLAGIGTEGAM
IAFGAFLLAHSLYKGALFLVAGALDHETGTKEILQMGGLRHAMPKTAVLAAVSALSLAGIPPLFGFLGKELMLESVLEAP
YLSAVLALLALGTAVLVVIVAGIVAIRPFFGERTETPKEPHEAPPGMLAGPAVLGTLSVLFGLFPFLPEGLIGAVASSLA
GEPVSVSLSLWHGINLPLIMSLFAIAAGAFLYTRWDQVREAMGRYDWLYERGPEAGYFRVMDALVWSADWQTRVLQNGYL
RHYITTTVLTTVLLVGYTLATRYDGGFSFGLEVYFYEWIIAGVLIASVLFACVTQSRLGAVASLGVMGFTVALIFILFSA
PDLGITQILVETLTVILLVLVLFRLPGFMDFSTPQQRVRDGVVAGVMGVLVTSLLLVTIDVQFAEPISAYHLDESVPLAH
GRNVVNVILVDYRALDTLGEIFVVALAAIGVYAMVKYRVTGERGAAEKEDTQ
>Mature_772_residues
MLAAVLSGFLLAVLVPWLQPVLGRRAGWAFAALPAVLTLYFATFLPQIVGGETVWMEYTWVPGLDVNLSFYVDGLSLLFA
LLISGIGTFVLLYASDYLDGDDRQPRFYVHILAFMASMLGLVLSDNLITLFIFWELTSITSYMLIGYDNEDAKARRYALQ
GLLVTVGGGLALLAGFIMLGIVGDTYTISELLEQGDVIREHEWYVPLTLLILLGAFTKSAQVPFHFWLPRAMAAPTPVSA
YLHSATMVKAGVFLLAKLHPALGDTALWMTLLPLVGAITMFTGAYFAVRSTGIKGVLANSTVMALGTLTMLAGIGTEGAM
IAFGAFLLAHSLYKGALFLVAGALDHETGTKEILQMGGLRHAMPKTAVLAAVSALSLAGIPPLFGFLGKELMLESVLEAP
YLSAVLALLALGTAVLVVIVAGIVAIRPFFGERTETPKEPHEAPPGMLAGPAVLGTLSVLFGLFPFLPEGLIGAVASSLA
GEPVSVSLSLWHGINLPLIMSLFAIAAGAFLYTRWDQVREAMGRYDWLYERGPEAGYFRVMDALVWSADWQTRVLQNGYL
RHYITTTVLTTVLLVGYTLATRYDGGFSFGLEVYFYEWIIAGVLIASVLFACVTQSRLGAVASLGVMGFTVALIFILFSA
PDLGITQILVETLTVILLVLVLFRLPGFMDFSTPQQRVRDGVVAGVMGVLVTSLLLVTIDVQFAEPISAYHLDESVPLAH
GRNVVNVILVDYRALDTLGEIFVVALAAIGVYAMVKYRVTGERGAAEKEDTQ

Specific function: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+) and/or Li(+) excretion and Na(+) resistance. Na(+)/H(+) antiport consumes a transmembrane electrical potential, and is thus inferred to be electrogenic. Does not transport K(+), Ca(2+) or Mg(2+

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=335, Percent_Identity=34.9253731343284, Blast_Score=162, Evalue=1e-39,
Organism=Escherichia coli, GI1788614, Length=370, Percent_Identity=34.5945945945946, Blast_Score=175, Evalue=1e-44,
Organism=Escherichia coli, GI1788829, Length=386, Percent_Identity=33.6787564766839, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788827, Length=499, Percent_Identity=26.25250501002, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI1788831, Length=338, Percent_Identity=28.4023668639053, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788613, Length=358, Percent_Identity=26.8156424581006, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI2367154, Length=290, Percent_Identity=30.3448275862069, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI145693160, Length=341, Percent_Identity=26.9794721407625, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 83197; Mature: 83197

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAAVLSGFLLAVLVPWLQPVLGRRAGWAFAALPAVLTLYFATFLPQIVGGETVWMEYTW
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
VPGLDVNLSFYVDGLSLLFALLISGIGTFVLLYASDYLDGDDRQPRFYVHILAFMASMLG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LVLSDNLITLFIFWELTSITSYMLIGYDNEDAKARRYALQGLLVTVGGGLALLAGFIMLG
HHHCCCCEEHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHH
IVGDTYTISELLEQGDVIREHEWYVPLTLLILLGAFTKSAQVPFHFWLPRAMAAPTPVSA
HCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCHHHCCCCCHHH
YLHSATMVKAGVFLLAKLHPALGDTALWMTLLPLVGAITMFTGAYFAVRSTGIKGVLANS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
TVMALGTLTMLAGIGTEGAMIAFGAFLLAHSLYKGALFLVAGALDHETGTKEILQMGGLR
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCH
HAMPKTAVLAAVSALSLAGIPPLFGFLGKELMLESVLEAPYLSAVLALLALGTAVLVVIV
HHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AGIVAIRPFFGERTETPKEPHEAPPGMLAGPAVLGTLSVLFGLFPFLPEGLIGAVASSLA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GEPVSVSLSLWHGINLPLIMSLFAIAAGAFLYTRWDQVREAMGRYDWLYERGPEAGYFRV
CCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
MDALVWSADWQTRVLQNGYLRHYITTTVLTTVLLVGYTLATRYDGGFSFGLEVYFYEWII
HHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AGVLIASVLFACVTQSRLGAVASLGVMGFTVALIFILFSAPDLGITQILVETLTVILLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VLFRLPGFMDFSTPQQRVRDGVVAGVMGVLVTSLLLVTIDVQFAEPISAYHLDESVPLAH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCC
GRNVVNVILVDYRALDTLGEIFVVALAAIGVYAMVKYRVTGERGAAEKEDTQ
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLAAVLSGFLLAVLVPWLQPVLGRRAGWAFAALPAVLTLYFATFLPQIVGGETVWMEYTW
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
VPGLDVNLSFYVDGLSLLFALLISGIGTFVLLYASDYLDGDDRQPRFYVHILAFMASMLG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LVLSDNLITLFIFWELTSITSYMLIGYDNEDAKARRYALQGLLVTVGGGLALLAGFIMLG
HHHCCCCEEHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHEECCHHHHHHHHHHHH
IVGDTYTISELLEQGDVIREHEWYVPLTLLILLGAFTKSAQVPFHFWLPRAMAAPTPVSA
HCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCHHHCCCCCHHH
YLHSATMVKAGVFLLAKLHPALGDTALWMTLLPLVGAITMFTGAYFAVRSTGIKGVLANS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
TVMALGTLTMLAGIGTEGAMIAFGAFLLAHSLYKGALFLVAGALDHETGTKEILQMGGLR
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCH
HAMPKTAVLAAVSALSLAGIPPLFGFLGKELMLESVLEAPYLSAVLALLALGTAVLVVIV
HHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AGIVAIRPFFGERTETPKEPHEAPPGMLAGPAVLGTLSVLFGLFPFLPEGLIGAVASSLA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
GEPVSVSLSLWHGINLPLIMSLFAIAAGAFLYTRWDQVREAMGRYDWLYERGPEAGYFRV
CCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
MDALVWSADWQTRVLQNGYLRHYITTTVLTTVLLVGYTLATRYDGGFSFGLEVYFYEWII
HHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AGVLIASVLFACVTQSRLGAVASLGVMGFTVALIFILFSAPDLGITQILVETLTVILLVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
VLFRLPGFMDFSTPQQRVRDGVVAGVMGVLVTSLLLVTIDVQFAEPISAYHLDESVPLAH
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCC
GRNVVNVILVDYRALDTLGEIFVVALAAIGVYAMVKYRVTGERGAAEKEDTQ
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]