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The map label for this gene is ligA

Identifier: 121998657

GI number: 121998657

Start: 2055209

End: 2057344

Strand: Reverse

Name: ligA

Synonym: Hhal_1878

Alternate gene names: 121998657

Gene position: 2057344-2055209 (Counterclockwise)

Preceding gene: 121998658

Following gene: 121998656

Centisome position: 76.81

GC content: 71.68

Gene sequence:

>2136_bases
GTGGCTGAACCCACCGCGACCCGCGAGCAGGCGCAGCAGCGCATGGCGCAGCTGCGCGGCGAGATCAGCGAGCACGACTA
CCACTACTACGTCCTCGATCGACCGACGATCTCGGATGCCGAGTACGACGCCCTGTTCCGGGAGCTCCAGCGCCTGGAGG
CCGCCTACCCCGACCTGGTCAGCTCCGACTCGCCCACCCAGCGGGTCGCGGGTACGGCCCAGGAGGGGTTTGAGGCGGTT
GTCCACGACACCCCCATGCTCTCCCTGGACAACGCCTTCGAGGCTGACGATCTGGTGGATTTCGACCGCCGTATCCGTGA
CCGGCTGGGCGTGGCCAGCGTCGTCTATACCGGTGAGCCGAAGCTCGACGGCATCTCGCTGAGCCTGACCTACCAGGATG
GCGAGCTGGTGCGGGCCGGGACCCGTGGCGACGGTCACACCGGCGAGGACGTGACCGCCAATGTGCGGACACTGCGCAGC
GTCCCCCTGCGCCTGCGTGGTGATGGCTACCCGGGGCGCCTGGAGGTCCGTGGCGAGGTGGTGATCCGCAAGGAGGACTT
CGAACGGCTCAATCAGGCGCGCCTCGACGCCGGCGAACGGCCCTTCGCCAATCCTCGTAACGCCGCGGCCGGGTCCCTGC
GCCAGCTCGACCCGCGCATCACGGCGCGGCGGCCGCTGACCCTGTTCACCTTCGGCGTCGGTGACCCGCTGGCCCTGGGG
GTGGAGAGCCATTGGCAGGTGCTGGAGTGCCTGGCCGGCTGGGGGTTTCGCACCACCGCCCCGCTGGAGCACCTGGAGGG
TGTGTCGGCCTGCCAGGCCTACCGGGATCGGCTCATCGAGGAGCGGGATGCGCTGCCCTTCGAGATCGACGGGGCCGTGT
TCAAGGTCGACGACTTGGCCGTTCGCGAGCAGCTCGGCTTCACCGCCCGCGCGCCGCGCTGGGCGCTGGCCTTCAAGCTG
CCGGCCCGCGAGGCGACCACCGTGGTCGAGGCGATCGTCCCCTCGGTGGGGCGCACCGGCAAGATCACCCCGCTGGCCCG
CCTGCAGCCGGTCGAGGTGAGTGGGGTGACCGTCGCCCGGGCCAGCCTCCACAACGCCGACGAACTCGCGCGTATGGACG
TGCGCGAGGGCGATACGGTGATGGTGCGCCGGGCCGGCGACGTGATCCCGCAGATCACCGACGTGGTGCTGGCGAAGCGG
CCGGAGGGGGCGCAGCCGTGGCGCTTCGAGGAGCGGGTGCAGGCGTGCCCGGAGTGCGGGTCGGCGGTTCAGCGCATCGA
GGGCGAGGCGGCCCACCGTTGCGTGGGCGGCCTCTACTGCCCGGCGCAGCGCGAGGGGGCCATCCTGCACTTCGCCTCGC
GGCGGGCCCTGGATATCGACGGACTCGGCGAGAAGATCGTCGAGCAGCTGGTGGCCAAGGGGTTGGTGGTGGACCTGGCC
GACCTGTTCCGGCTTGGCCACGCCCAGCTGGCCGGGCTCGAGCGCATGGGCGACAAGTCCGCCGACAACCTGCTCGCCGC
CCTGGACCGGGCGCGGAGCACAACGCTGGCGCGTTTCCTCTACGCCCTGGGGATCCCCCATGTCGGCGAGGTGACCGCCC
GCCGCCTGGCCGAGGCGGCCTACGGCCTGATGGTCGCGGTCCCCCGTGGCGATCGGGCGGCGCTGCGCGCGGCAGCCGGT
CTGGATGCGCAAACGGCCATCACCAGCGAGGCGCTGCTGCGGGTCATGGCGGCGCCGGCCGACGATCTGGAGGCCATCGA
AGACGTGGGGCCGGTGGTCGCCCGGGCCATCGCCGGCTTCTTCGCCGAGCCGCACAACCGGCAGGTGGTGGATCACCTCT
GCGCCGCCGGGGTCCACTGGCCCGAGCCGGATCCGCCCGAGCAGGCCGAGTCGGTTTCGTCGCTGGCCGGGCGGGCCTTT
GTGGTGACCGGCACCCTGGAGACCATGACCCGGGACGAGGCCAAGGCCGCTATCGAGGCGTGCGGCGGCCGGGTGACCGG
CAGCGTCTCGCGCAAGACCGACTACCTGGTGGCCGGGGCCGATGCCGGCAGCAAACTGAACAAGGCCCGCGAACTCGGTG
TCGAAGTCGTGAACGAATCCGGCCTGCGTGAACTCCTCGCTGGCGCCGGGGCCTGA

Upstream 100 bases:

>100_bases
AGATCGCCGAGCACATGCGTGAGCAACTGCGCGAGCACCGGCGGCAGCTGCATATCGCCGTGCACAAGCGTTCGTAGACT
CGACCCCGACTGGAGAGCCC

Downstream 100 bases:

>100_bases
CCGTGACCGCAGCCGGTCTCGGCCCGTATCCACCCGGAGTGGCGGAGGGGGTTGGTATACTTTGCCGCAGCTCCAGACCA
GGTAAGTCGACCGATATTCC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MAEPTATREQAQQRMAQLRGEISEHDYHYYVLDRPTISDAEYDALFRELQRLEAAYPDLVSSDSPTQRVAGTAQEGFEAV
VHDTPMLSLDNAFEADDLVDFDRRIRDRLGVASVVYTGEPKLDGISLSLTYQDGELVRAGTRGDGHTGEDVTANVRTLRS
VPLRLRGDGYPGRLEVRGEVVIRKEDFERLNQARLDAGERPFANPRNAAAGSLRQLDPRITARRPLTLFTFGVGDPLALG
VESHWQVLECLAGWGFRTTAPLEHLEGVSACQAYRDRLIEERDALPFEIDGAVFKVDDLAVREQLGFTARAPRWALAFKL
PAREATTVVEAIVPSVGRTGKITPLARLQPVEVSGVTVARASLHNADELARMDVREGDTVMVRRAGDVIPQITDVVLAKR
PEGAQPWRFEERVQACPECGSAVQRIEGEAAHRCVGGLYCPAQREGAILHFASRRALDIDGLGEKIVEQLVAKGLVVDLA
DLFRLGHAQLAGLERMGDKSADNLLAALDRARSTTLARFLYALGIPHVGEVTARRLAEAAYGLMVAVPRGDRAALRAAAG
LDAQTAITSEALLRVMAAPADDLEAIEDVGPVVARAIAGFFAEPHNRQVVDHLCAAGVHWPEPDPPEQAESVSSLAGRAF
VVTGTLETMTRDEAKAAIEACGGRVTGSVSRKTDYLVAGADAGSKLNKARELGVEVVNESGLRELLAGAGA

Sequences:

>Translated_711_residues
MAEPTATREQAQQRMAQLRGEISEHDYHYYVLDRPTISDAEYDALFRELQRLEAAYPDLVSSDSPTQRVAGTAQEGFEAV
VHDTPMLSLDNAFEADDLVDFDRRIRDRLGVASVVYTGEPKLDGISLSLTYQDGELVRAGTRGDGHTGEDVTANVRTLRS
VPLRLRGDGYPGRLEVRGEVVIRKEDFERLNQARLDAGERPFANPRNAAAGSLRQLDPRITARRPLTLFTFGVGDPLALG
VESHWQVLECLAGWGFRTTAPLEHLEGVSACQAYRDRLIEERDALPFEIDGAVFKVDDLAVREQLGFTARAPRWALAFKL
PAREATTVVEAIVPSVGRTGKITPLARLQPVEVSGVTVARASLHNADELARMDVREGDTVMVRRAGDVIPQITDVVLAKR
PEGAQPWRFEERVQACPECGSAVQRIEGEAAHRCVGGLYCPAQREGAILHFASRRALDIDGLGEKIVEQLVAKGLVVDLA
DLFRLGHAQLAGLERMGDKSADNLLAALDRARSTTLARFLYALGIPHVGEVTARRLAEAAYGLMVAVPRGDRAALRAAAG
LDAQTAITSEALLRVMAAPADDLEAIEDVGPVVARAIAGFFAEPHNRQVVDHLCAAGVHWPEPDPPEQAESVSSLAGRAF
VVTGTLETMTRDEAKAAIEACGGRVTGSVSRKTDYLVAGADAGSKLNKARELGVEVVNESGLRELLAGAGA
>Mature_710_residues
AEPTATREQAQQRMAQLRGEISEHDYHYYVLDRPTISDAEYDALFRELQRLEAAYPDLVSSDSPTQRVAGTAQEGFEAVV
HDTPMLSLDNAFEADDLVDFDRRIRDRLGVASVVYTGEPKLDGISLSLTYQDGELVRAGTRGDGHTGEDVTANVRTLRSV
PLRLRGDGYPGRLEVRGEVVIRKEDFERLNQARLDAGERPFANPRNAAAGSLRQLDPRITARRPLTLFTFGVGDPLALGV
ESHWQVLECLAGWGFRTTAPLEHLEGVSACQAYRDRLIEERDALPFEIDGAVFKVDDLAVREQLGFTARAPRWALAFKLP
AREATTVVEAIVPSVGRTGKITPLARLQPVEVSGVTVARASLHNADELARMDVREGDTVMVRRAGDVIPQITDVVLAKRP
EGAQPWRFEERVQACPECGSAVQRIEGEAAHRCVGGLYCPAQREGAILHFASRRALDIDGLGEKIVEQLVAKGLVVDLAD
LFRLGHAQLAGLERMGDKSADNLLAALDRARSTTLARFLYALGIPHVGEVTARRLAEAAYGLMVAVPRGDRAALRAAAGL
DAQTAITSEALLRVMAAPADDLEAIEDVGPVVARAIAGFFAEPHNRQVVDHLCAAGVHWPEPDPPEQAESVSSLAGRAFV
VTGTLETMTRDEAKAAIEACGGRVTGSVSRKTDYLVAGADAGSKLNKARELGVEVVNESGLRELLAGAGA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=82, Percent_Identity=52.4390243902439, Blast_Score=81, Evalue=4e-15,
Organism=Escherichia coli, GI1788750, Length=702, Percent_Identity=49.5726495726496, Blast_Score=654, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=525, Percent_Identity=24.952380952381, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_HALHL (A1WY80)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003444.1
- ProteinModelPortal:   A1WY80
- STRING:   A1WY80
- GeneID:   4710875
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1878
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   A1WY80
- ProtClustDB:   CLSK2516571
- BioCyc:   HHAL349124:HHAL_1878-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76889; Mature: 76758

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 121-121 BINDING 119-119 BINDING 142-142 BINDING 179-179 BINDING 295-295 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPTATREQAQQRMAQLRGEISEHDYHYYVLDRPTISDAEYDALFRELQRLEAAYPDLV
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHH
SSDSPTQRVAGTAQEGFEAVVHDTPMLSLDNAFEADDLVDFDRRIRDRLGVASVVYTGEP
CCCCCHHHHHCCHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCC
KLDGISLSLTYQDGELVRAGTRGDGHTGEDVTANVRTLRSVPLRLRGDGYPGRLEVRGEV
CCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCEEEEECEE
VIRKEDFERLNQARLDAGERPFANPRNAAAGSLRQLDPRITARRPLTLFTFGVGDPLALG
EEEHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCCEEEEEECCCCCEEEC
VESHWQVLECLAGWGFRTTAPLEHLEGVSACQAYRDRLIEERDALPFEIDGAVFKVDDLA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEHHHH
VREQLGFTARAPRWALAFKLPAREATTVVEAIVPSVGRTGKITPLARLQPVEVSGVTVAR
HHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEECCEEEEH
ASLHNADELARMDVREGDTVMVRRAGDVIPQITDVVLAKRPEGAQPWRFEERVQACPECG
HHHCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SAVQRIEGEAAHRCVGGLYCPAQREGAILHFASRRALDIDGLGEKIVEQLVAKGLVVDLA
HHHHHHCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHH
DLFRLGHAQLAGLERMGDKSADNLLAALDRARSTTLARFLYALGIPHVGEVTARRLAEAA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YGLMVAVPRGDRAALRAAAGLDAQTAITSEALLRVMAAPADDLEAIEDVGPVVARAIAGF
CCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FAEPHNRQVVDHLCAAGVHWPEPDPPEQAESVSSLAGRAFVVTGTLETMTRDEAKAAIEA
HCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
CGGRVTGSVSRKTDYLVAGADAGSKLNKARELGVEVVNESGLRELLAGAGA
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEPTATREQAQQRMAQLRGEISEHDYHYYVLDRPTISDAEYDALFRELQRLEAAYPDLV
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHH
SSDSPTQRVAGTAQEGFEAVVHDTPMLSLDNAFEADDLVDFDRRIRDRLGVASVVYTGEP
CCCCCHHHHHCCHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCC
KLDGISLSLTYQDGELVRAGTRGDGHTGEDVTANVRTLRSVPLRLRGDGYPGRLEVRGEV
CCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCEEEEECEE
VIRKEDFERLNQARLDAGERPFANPRNAAAGSLRQLDPRITARRPLTLFTFGVGDPLALG
EEEHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCCEEEEEECCCCCEEEC
VESHWQVLECLAGWGFRTTAPLEHLEGVSACQAYRDRLIEERDALPFEIDGAVFKVDDLA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEHHHH
VREQLGFTARAPRWALAFKLPAREATTVVEAIVPSVGRTGKITPLARLQPVEVSGVTVAR
HHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHCCCCEEECCEEEEH
ASLHNADELARMDVREGDTVMVRRAGDVIPQITDVVLAKRPEGAQPWRFEERVQACPECG
HHHCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SAVQRIEGEAAHRCVGGLYCPAQREGAILHFASRRALDIDGLGEKIVEQLVAKGLVVDLA
HHHHHHCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHH
DLFRLGHAQLAGLERMGDKSADNLLAALDRARSTTLARFLYALGIPHVGEVTARRLAEAA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
YGLMVAVPRGDRAALRAAAGLDAQTAITSEALLRVMAAPADDLEAIEDVGPVVARAIAGF
CCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FAEPHNRQVVDHLCAAGVHWPEPDPPEQAESVSSLAGRAFVVTGTLETMTRDEAKAAIEA
HCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
CGGRVTGSVSRKTDYLVAGADAGSKLNKARELGVEVVNESGLRELLAGAGA
CCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA