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The map label for this gene is tpiA

Identifier: 121998546

GI number: 121998546

Start: 1940184

End: 1940933

Strand: Reverse

Name: tpiA

Synonym: Hhal_1767

Alternate gene names: 121998546

Gene position: 1940933-1940184 (Counterclockwise)

Preceding gene: 121998547

Following gene: 121998545

Centisome position: 72.46

GC content: 67.2

Gene sequence:

>750_bases
ATGCGGCGCAAGATCATAGCTGGAAACTGGAAGATGAACGGCGACCAGGATCTGGTCCGCCGCGTGGCGGAGCGTGCTGC
CGACAGTGCGGGGGATGCCGAGTTGGCCGTCTGTCCGCCGTACCCGCTGCTGGCCGCGGCGGCTTCACAGCTGCCCTTCG
GTGTCGCACTGGGCGCCCAGGACGTGAGTGAGTACGACTCGGGCGCCTACACCGGTGAGGTGTCGGCGAGCATGTTGCTC
GAAGCCGGATGCCGCTACGTGATCGTCGGCCACTCCGAGCGCCGGACGCTCTATGGCGAGGACAATGGCCGGGTGGCCGG
CAAGTTCGTGGCGGCGCGCAATGCCGGGCTGACCCCGATCCTGTGCGTTGGTGAGACCCTGGCCGAGCGCGACGCTGAGC
GCACCGAGTCCGTGGTCGGTGAACAGCTCGACGCTGTGATGGACGCCGTCGGCGGCGCCACCTTTCAGGGCGCGGTGATC
GCCTACGAGCCCGTGTGGGCCATTGGTACCGGACGTACCGCCACGCCGGAACAGGCGCAGGCGGTTCATGCCTTTATTCG
GCAGCGCGTCCAGGAGCGCGACGCCGGCGAGATCGCCGATCAACTGCCCATCCTCTACGGGGGCAGCATGAAAGCCGACA
ACGCTGCCGAGCTGCTGGCCCAGCCGGATATCGACGGGGGTCTGATCGGTGGCGCATCGCTGGATCCCGACAGTTTCCTT
TCGATTTACAACGCAGCCGCGGAGGGCTGA

Upstream 100 bases:

>100_bases
CCGCTCGGGGTGCGGCGACCGACCCGATTTGATTTCAGGCGTTTGTGTAGATAGGCTTCGTCAGCTTCACAGGAATCGAT
AGACAGGGTTGGGTCTAGGC

Downstream 100 bases:

>100_bases
GGTAGACCCATGTTTGAAGCGTTGCTTGCCGTCCATATCGTCATCGCCGTAGCCCTGGTGGTGCTCGTGCTGCTGAACCA
GGGAAAGGGCGCTGACATGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRRKIIAGNWKMNGDQDLVRRVAERAADSAGDAELAVCPPYPLLAAAASQLPFGVALGAQDVSEYDSGAYTGEVSASMLL
EAGCRYVIVGHSERRTLYGEDNGRVAGKFVAARNAGLTPILCVGETLAERDAERTESVVGEQLDAVMDAVGGATFQGAVI
AYEPVWAIGTGRTATPEQAQAVHAFIRQRVQERDAGEIADQLPILYGGSMKADNAAELLAQPDIDGGLIGGASLDPDSFL
SIYNAAAEG

Sequences:

>Translated_249_residues
MRRKIIAGNWKMNGDQDLVRRVAERAADSAGDAELAVCPPYPLLAAAASQLPFGVALGAQDVSEYDSGAYTGEVSASMLL
EAGCRYVIVGHSERRTLYGEDNGRVAGKFVAARNAGLTPILCVGETLAERDAERTESVVGEQLDAVMDAVGGATFQGAVI
AYEPVWAIGTGRTATPEQAQAVHAFIRQRVQERDAGEIADQLPILYGGSMKADNAAELLAQPDIDGGLIGGASLDPDSFL
SIYNAAAEG
>Mature_249_residues
MRRKIIAGNWKMNGDQDLVRRVAERAADSAGDAELAVCPPYPLLAAAASQLPFGVALGAQDVSEYDSGAYTGEVSASMLL
EAGCRYVIVGHSERRTLYGEDNGRVAGKFVAARNAGLTPILCVGETLAERDAERTESVVGEQLDAVMDAVGGATFQGAVI
AYEPVWAIGTGRTATPEQAQAVHAFIRQRVQERDAGEIADQLPILYGGSMKADNAAELLAQPDIDGGLIGGASLDPDSFL
SIYNAAAEG

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=42.2764227642276, Blast_Score=187, Evalue=6e-48,
Organism=Homo sapiens, GI226529917, Length=246, Percent_Identity=42.2764227642276, Blast_Score=187, Evalue=9e-48,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=51.792828685259, Blast_Score=244, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17536593, Length=246, Percent_Identity=47.9674796747967, Blast_Score=223, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=42.1052631578947, Blast_Score=185, Evalue=6e-48,
Organism=Drosophila melanogaster, GI28572008, Length=246, Percent_Identity=45.5284552845528, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI28572006, Length=246, Percent_Identity=45.5284552845528, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI28572004, Length=246, Percent_Identity=45.5284552845528, Blast_Score=207, Evalue=5e-54,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_HALHL (A1WXW9)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003333.1
- ProteinModelPortal:   A1WXW9
- SMR:   A1WXW9
- STRING:   A1WXW9
- GeneID:   4711020
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1767
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   A1WXW9
- ProtClustDB:   PRK00042
- BioCyc:   HHAL349124:HHAL_1767-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26071; Mature: 26071

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 91-91 ACT_SITE 163-163 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKIIAGNWKMNGDQDLVRRVAERAADSAGDAELAVCPPYPLLAAAASQLPFGVALGAQ
CCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHCCCEEEECCH
DVSEYDSGAYTGEVSASMLLEAGCRYVIVGHSERRTLYGEDNGRVAGKFVAARNAGLTPI
HHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEEEEEEEECCCCCCEE
LCVGETLAERDAERTESVVGEQLDAVMDAVGGATFQGAVIAYEPVWAIGTGRTATPEQAQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCCCCHHHHH
AVHAFIRQRVQERDAGEIADQLPILYGGSMKADNAAELLAQPDIDGGLIGGASLDPDSFL
HHHHHHHHHHHHCCHHHHHHHCCEEECCCCCCCHHHHHHHCCCCCCCEECCCCCCHHHHH
SIYNAAAEG
HHHHHHCCC
>Mature Secondary Structure
MRRKIIAGNWKMNGDQDLVRRVAERAADSAGDAELAVCPPYPLLAAAASQLPFGVALGAQ
CCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHCCCEEEECCH
DVSEYDSGAYTGEVSASMLLEAGCRYVIVGHSERRTLYGEDNGRVAGKFVAARNAGLTPI
HHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEECCCCCEEEEEEEEECCCCCCEE
LCVGETLAERDAERTESVVGEQLDAVMDAVGGATFQGAVIAYEPVWAIGTGRTATPEQAQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCCCCCHHHHH
AVHAFIRQRVQERDAGEIADQLPILYGGSMKADNAAELLAQPDIDGGLIGGASLDPDSFL
HHHHHHHHHHHHCCHHHHHHHCCEEECCCCCCCHHHHHHHCCCCCCCEECCCCCCHHHHH
SIYNAAAEG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA