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The map label for this gene is nuoB

Identifier: 121998543

GI number: 121998543

Start: 1938492

End: 1938968

Strand: Reverse

Name: nuoB

Synonym: Hhal_1764

Alternate gene names: 121998543

Gene position: 1938968-1938492 (Counterclockwise)

Preceding gene: 121998544

Following gene: 121998542

Centisome position: 72.39

GC content: 62.05

Gene sequence:

>477_bases
ATGGGAGTAGAAGGCATCCTAGAGCGGGGGTACGTGACCACCTCCGCCGATAAGTTGATCAACTGGGCGCGGACCGGTTC
GCTTTGGCCCATGACCTTCGGTCTGGCCTGCTGTGCGGTCGAGATGATGCACACTGCGGCGGGTCGATACGACATGGACC
GCAATGGCCTGATCTTCCGGCCCAGCCCCCGACAGTCCGACGTAATGATCGTGGCGGGCACACTCTGTAACAAGATGGCC
CCGGCCCTGCGTAAGGTCTACGACCAGATGCCCGAGCCGAAATGGGTGATCTCCATGGGCTCGTGCGCCAACGGTGGGGG
ATACTACCACTACTCCTACTCGGTCACCCGGGGCTGTGACCGCATCGTCCCCGTAGACATCTACGTACCCGGCTGTCCGC
CGACGGCCGAGGCGCTCTACTACGGCATTCTGCAGCTGCAGAACAAGATCCGCCGGACCAACACCATCGCACGTTGA

Upstream 100 bases:

>100_bases
TGTTGGACGATATAGGCCTGTTCGGCATCTTGGCCATGGGCCTGTTCGTTGGACTTCTTGTGATCGGTTTGCTCTACGAG
TGGCGTAAAGGGGCGCTGGA

Downstream 100 bases:

>100_bases
GGGAAAGTCATGGCTGAGCCCGAGAGCCTCTTAGAGCAGCTCCGCGGCCATTTCGGTGGCCGTTTGACCGATTGCTGGCT
CGAGCGCGGCGAGGTGACGG

Product: NADH-quinone oxidoreductase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MGVEGILERGYVTTSADKLINWARTGSLWPMTFGLACCAVEMMHTAAGRYDMDRNGLIFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMPEPKWVISMGSCANGGGYYHYSYSVTRGCDRIVPVDIYVPGCPPTAEALYYGILQLQNKIRRTNTIAR

Sequences:

>Translated_158_residues
MGVEGILERGYVTTSADKLINWARTGSLWPMTFGLACCAVEMMHTAAGRYDMDRNGLIFRPSPRQSDVMIVAGTLCNKMA
PALRKVYDQMPEPKWVISMGSCANGGGYYHYSYSVTRGCDRIVPVDIYVPGCPPTAEALYYGILQLQNKIRRTNTIAR
>Mature_157_residues
GVEGILERGYVTTSADKLINWARTGSLWPMTFGLACCAVEMMHTAAGRYDMDRNGLIFRPSPRQSDVMIVAGTLCNKMAP
ALRKVYDQMPEPKWVISMGSCANGGGYYHYSYSVTRGCDRIVPVDIYVPGCPPTAEALYYGILQLQNKIRRTNTIAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=146, Percent_Identity=81.5068493150685, Blast_Score=259, Evalue=7e-70,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=47.6510067114094, Blast_Score=156, Evalue=6e-40,
Organism=Escherichia coli, GI1789074, Length=107, Percent_Identity=43.9252336448598, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788834, Length=126, Percent_Identity=37.3015873015873, Blast_Score=88, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=74.6478873239437, Blast_Score=241, Evalue=1e-64,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=81.4285714285714, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=81.4285714285714, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=82.1428571428571, Blast_Score=253, Evalue=5e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_HALHL (A1WXW6)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003330.1
- ProteinModelPortal:   A1WXW6
- SMR:   A1WXW6
- STRING:   A1WXW6
- GeneID:   4711083
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1764
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   A1WXW6
- BioCyc:   HHAL349124:HHAL_1764-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 17553; Mature: 17421

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVEGILERGYVTTSADKLINWARTGSLWPMTFGLACCAVEMMHTAAGRYDMDRNGLIFR
CCCHHHHHCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPKWVISMGSCANGGGYYHYSYSVTRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEHHCCCC
RIVPVDIYVPGCPPTAEALYYGILQLQNKIRRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GVEGILERGYVTTSADKLINWARTGSLWPMTFGLACCAVEMMHTAAGRYDMDRNGLIFR
CCHHHHHCCEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEC
PSPRQSDVMIVAGTLCNKMAPALRKVYDQMPEPKWVISMGSCANGGGYYHYSYSVTRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEHHCCCC
RIVPVDIYVPGCPPTAEALYYGILQLQNKIRRTNTIAR
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA