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The map label for this gene is nuoM [H]

Identifier: 121998532

GI number: 121998532

Start: 1926230

End: 1927747

Strand: Reverse

Name: nuoM [H]

Synonym: Hhal_1753

Alternate gene names: 121998532

Gene position: 1927747-1926230 (Counterclockwise)

Preceding gene: 121998533

Following gene: 121998531

Centisome position: 71.97

GC content: 65.68

Gene sequence:

>1518_bases
ATGTTCGACGGTTGGTTGCTTAGCCTGATTATCTGGCTGCCGATCCTCGGCGGTGCCGCGGTGCTGGCGGTGGGCCGGCG
CTCGGAGGTGGCGGTCCGGGAATTGGCCACCGGTGTGGCGGGGCTGACATTCCTGTTCAGCCTGGTCCTCTGGTTCGCCT
TCGAGCCCGGGGGCGGCATGCAGTTCGTGGAGAAGCGCGACTGGGTGCCGACCTTCGACATCCATTACCACCTCGGTGTG
GACGGGATCTCGATGCCGCTGGTGGTGCTGACCGCCTTTGCAACGCTGCTGGTGGTGATCGCCGGACGCAGCGGCATCAC
CTACCGGCCGGCGCAGTACATGGGCGCCTTCCTGGTCATGGAAGGGTTGATGATCGGCGTCTTCGCCGCGCTGGACGCCA
TCCTCTTCTACGTCTTCTGGGAGGCGATGCTGGTGCCGATGTTCCTGCTCATCGGCATGTGGGGCGGCGCCAACCGCATC
TACGCGACCATCAAGTTCTTCCTCTACACCTTCCTCGGCTCCGTGCTGATGCTCATCGCCTTCCTGTACCTGCGCTGGGA
GGCGGGCAGCTTCGGTGTGCTCGACTTCCACGGCCTGAGCATCGGGCTGGAGGCGCAGATCCTGATCTTCCTGGCGCTGC
TGGCGGCTTTCGCCGTCAAGGTGCCCATGTGGCCGGTGCACACCTGGCTGCCGGACGCGCACACCGAGGCGCCGACCGGG
GGTTCGGTGATCCTGGCGGCGATCACCCTGAAGATGGGGGCCTACGGGTTCCTGCGCTTTGCGCTGCCCATCGCACCGGA
AGCGAGCCAGGAGCTGGCCTGGCTGGTCATTGCCATGTCGCTGATCGCCATCGTCTACATCGGTCTGGTGGCGATGGTGC
AGGAAGACATGAAGCGGCTGATCGCCTACTCGTCGATCGCCCACATGGGCTTCGCCACCCTTGGGTTCTTCGTGATCTTC
CAGGCCGGGTTGGGTGCCGAGGCAGGGGACGGTGCGCTGATGGCGATGACCGGCGGCTACGTCCAGCTCATCTCCCACGG
TTTCATCTCGGCGGCGCTGTTCCTCTGCGTCGGGGTGCTCTACGACCGGATGCACACCCGGAATATTGCCGACTACGGCG
GGGTGATCAACCGCATGCCGATCTTCGGCGCCCTGTTCGTGGTCTTTGCCATGGCCAACGCGGGCCTCCCGGGGACGTCG
GGCTTTGTCGGCGAGTTCCTGGTCATCCTCGGCAGCTACCAGGCCAGTTTCTGGTACGCCGCCATCGCTGGTCTGACCCT
GATCGTGGGGGCTGCCTACTCGCTGTGGCTGGTCAAGCGGGTGGTGTTCGGGCCGGTGGCCAATGAGCGGGTGGCGGCTC
TGAACGACCTCGACCGGCGCGAGTTCCTGCTGCTTGGCGTGCTCGCGGTGCTGGTGATCGGTGTCGGCGTCTACCCGCAG
GCGCTGGTCGCCGTGGCGGAGCCGACCCTGGTCGAGTTGTTGAACGACGTCTCTGCAGCGCTCCCGGCTGCCCGGTAA

Upstream 100 bases:

>100_bases
CATCGGCGTGCTGCTGCTGATCTCATTCTTCGTTGTGCGAACGCTGTTCGCCTAGTGCGCGGCTTCGGGACGGACGCGCA
AGAACGCAGGGGAAGAACGG

Downstream 100 bases:

>100_bases
GGAAGGAGAGGCAATGAGCTTCGAGACGCCCGATTTCTCCCTGGCGCTGCCGGAAATCTGGTTGCTGGCGGCCACCTGCG
GTGTGCTGGTGGTGGACCTC

Product: proton-translocating NADH-quinone oxidoreductase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MFDGWLLSLIIWLPILGGAAVLAVGRRSEVAVRELATGVAGLTFLFSLVLWFAFEPGGGMQFVEKRDWVPTFDIHYHLGV
DGISMPLVVLTAFATLLVVIAGRSGITYRPAQYMGAFLVMEGLMIGVFAALDAILFYVFWEAMLVPMFLLIGMWGGANRI
YATIKFFLYTFLGSVLMLIAFLYLRWEAGSFGVLDFHGLSIGLEAQILIFLALLAAFAVKVPMWPVHTWLPDAHTEAPTG
GSVILAAITLKMGAYGFLRFALPIAPEASQELAWLVIAMSLIAIVYIGLVAMVQEDMKRLIAYSSIAHMGFATLGFFVIF
QAGLGAEAGDGALMAMTGGYVQLISHGFISAALFLCVGVLYDRMHTRNIADYGGVINRMPIFGALFVVFAMANAGLPGTS
GFVGEFLVILGSYQASFWYAAIAGLTLIVGAAYSLWLVKRVVFGPVANERVAALNDLDRREFLLLGVLAVLVIGVGVYPQ
ALVAVAEPTLVELLNDVSAALPAAR

Sequences:

>Translated_505_residues
MFDGWLLSLIIWLPILGGAAVLAVGRRSEVAVRELATGVAGLTFLFSLVLWFAFEPGGGMQFVEKRDWVPTFDIHYHLGV
DGISMPLVVLTAFATLLVVIAGRSGITYRPAQYMGAFLVMEGLMIGVFAALDAILFYVFWEAMLVPMFLLIGMWGGANRI
YATIKFFLYTFLGSVLMLIAFLYLRWEAGSFGVLDFHGLSIGLEAQILIFLALLAAFAVKVPMWPVHTWLPDAHTEAPTG
GSVILAAITLKMGAYGFLRFALPIAPEASQELAWLVIAMSLIAIVYIGLVAMVQEDMKRLIAYSSIAHMGFATLGFFVIF
QAGLGAEAGDGALMAMTGGYVQLISHGFISAALFLCVGVLYDRMHTRNIADYGGVINRMPIFGALFVVFAMANAGLPGTS
GFVGEFLVILGSYQASFWYAAIAGLTLIVGAAYSLWLVKRVVFGPVANERVAALNDLDRREFLLLGVLAVLVIGVGVYPQ
ALVAVAEPTLVELLNDVSAALPAAR
>Mature_505_residues
MFDGWLLSLIIWLPILGGAAVLAVGRRSEVAVRELATGVAGLTFLFSLVLWFAFEPGGGMQFVEKRDWVPTFDIHYHLGV
DGISMPLVVLTAFATLLVVIAGRSGITYRPAQYMGAFLVMEGLMIGVFAALDAILFYVFWEAMLVPMFLLIGMWGGANRI
YATIKFFLYTFLGSVLMLIAFLYLRWEAGSFGVLDFHGLSIGLEAQILIFLALLAAFAVKVPMWPVHTWLPDAHTEAPTG
GSVILAAITLKMGAYGFLRFALPIAPEASQELAWLVIAMSLIAIVYIGLVAMVQEDMKRLIAYSSIAHMGFATLGFFVIF
QAGLGAEAGDGALMAMTGGYVQLISHGFISAALFLCVGVLYDRMHTRNIADYGGVINRMPIFGALFVVFAMANAGLPGTS
GFVGEFLVILGSYQASFWYAAIAGLTLIVGAAYSLWLVKRVVFGPVANERVAALNDLDRREFLLLGVLAVLVIGVGVYPQ
ALVAVAEPTLVELLNDVSAALPAAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=345, Percent_Identity=31.0144927536232, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI251831117, Length=373, Percent_Identity=23.8605898123324, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1788613, Length=523, Percent_Identity=36.7112810707457, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1788827, Length=285, Percent_Identity=29.1228070175439, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788829, Length=415, Percent_Identity=27.9518072289157, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788831, Length=204, Percent_Identity=28.921568627451, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI145693160, Length=364, Percent_Identity=27.4725274725275, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788614, Length=458, Percent_Identity=27.0742358078603, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI2367154, Length=251, Percent_Identity=27.8884462151394, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54411; Mature: 54411

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDGWLLSLIIWLPILGGAAVLAVGRRSEVAVRELATGVAGLTFLFSLVLWFAFEPGGGM
CCCHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QFVEKRDWVPTFDIHYHLGVDGISMPLVVLTAFATLLVVIAGRSGITYRPAQYMGAFLVM
HHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
EGLMIGVFAALDAILFYVFWEAMLVPMFLLIGMWGGANRIYATIKFFLYTFLGSVLMLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FLYLRWEAGSFGVLDFHGLSIGLEAQILIFLALLAAFAVKVPMWPVHTWLPDAHTEAPTG
HHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCC
GSVILAAITLKMGAYGFLRFALPIAPEASQELAWLVIAMSLIAIVYIGLVAMVQEDMKRL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAYSSIAHMGFATLGFFVIFQAGLGAEAGDGALMAMTGGYVQLISHGFISAALFLCVGVL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
YDRMHTRNIADYGGVINRMPIFGALFVVFAMANAGLPGTSGFVGEFLVILGSYQASFWYA
HHHHHHCCCHHHCCHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHH
AIAGLTLIVGAAYSLWLVKRVVFGPVANERVAALNDLDRREFLLLGVLAVLVIGVGVYPQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
ALVAVAEPTLVELLNDVSAALPAAR
HHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFDGWLLSLIIWLPILGGAAVLAVGRRSEVAVRELATGVAGLTFLFSLVLWFAFEPGGGM
CCCHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
QFVEKRDWVPTFDIHYHLGVDGISMPLVVLTAFATLLVVIAGRSGITYRPAQYMGAFLVM
HHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
EGLMIGVFAALDAILFYVFWEAMLVPMFLLIGMWGGANRIYATIKFFLYTFLGSVLMLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FLYLRWEAGSFGVLDFHGLSIGLEAQILIFLALLAAFAVKVPMWPVHTWLPDAHTEAPTG
HHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCC
GSVILAAITLKMGAYGFLRFALPIAPEASQELAWLVIAMSLIAIVYIGLVAMVQEDMKRL
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAYSSIAHMGFATLGFFVIFQAGLGAEAGDGALMAMTGGYVQLISHGFISAALFLCVGVL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
YDRMHTRNIADYGGVINRMPIFGALFVVFAMANAGLPGTSGFVGEFLVILGSYQASFWYA
HHHHHHCCCHHHCCHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHH
AIAGLTLIVGAAYSLWLVKRVVFGPVANERVAALNDLDRREFLLLGVLAVLVIGVGVYPQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
ALVAVAEPTLVELLNDVSAALPAAR
HHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]