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The map label for this gene is tgt [H]

Identifier: 121998478

GI number: 121998478

Start: 1855016

End: 1856164

Strand: Reverse

Name: tgt [H]

Synonym: Hhal_1699

Alternate gene names: 121998478

Gene position: 1856164-1855016 (Counterclockwise)

Preceding gene: 121998479

Following gene: 121998477

Centisome position: 69.3

GC content: 67.28

Gene sequence:

>1149_bases
GTGCAACTGGATGGGGCGGTGACGGACACCGGGGCGTTGCGCTTTGAGCGCATGGCGACCGACGGTGCGGCGCGCCGTGG
GCGGCTGCATCTGCCCCGCGGGACTATCGAGACCCCGGCCTTCATGCCGGTCGGGACCTACGGTACGGTCAAGGGGCTGA
CGCCGGAGGAGCTGCGCGAGCTGGGCGCAGAGATCATCCTCGGCAACACCTTCCATCTATGGCTGAGGCCGGGGACCGAG
GTGGTTCGCCTGCACGGCGATCTCCACGACTTCATGAACTGGCAGGGGCCGATCCTCACTGACTCGGGCGGCTTCCAGGT
CTACAGCCTTGGCGAGCTGCGCAAGGTCACTGAGGAGGGCGTCCACTTCCGTTCGCCGGTGGATGGGGCCCGTGTCTTCA
TGGGGCCCGAGGAGTCGATGGCGGTTCAGCGCGAGCTGGGTTCGGATATCGTCATGATTTTCGACGAGTGTCCCCCGTAT
CCGGCGGAGCGTTCGACGGTGCAGCGCTCCATGGAGTTATCGCTGCGCTGGGCTGCGCGCAGTCGCCAGGCCCACGGCGA
CAACCCAGCGGCGCTGTTCGGGATCGTCCAGGGCGGGGTCTACCCGGAATTGCGCCGGGCCTCGCTGCAGGGACTGATGG
ACATCGGCTTCGACGGCTACGCCATCGGTGGTCTCTCGGTGGGAGAGCCACGGGAGGAGCGGGATGCCGTGCTGGAAGAT
CTCGAGCCGCGGCTGCCTGTGGATCGGCCCCGCTACCTGATGGGCGTCGGCAAGCCGGAGGACTTGGTCGAGTGCGTGCG
CCGCGGGGTGGACCTGTTCGACTGCGTGATCCCGACGCGCAATGCGCGCAACGGCTTTCTGTTCACCTCCGAGGGTCTCG
TCCGGCTTCGCAACAGTCGCTATCGCACCGATACCGCCCCGGTGGATCCGGCCTGCGACTGCTACACCTGCCGCAACTAC
TCCCGGGCCTACCTCAAGCACCTGGAGCGGTGCAACGAGATGCTGGGGGCGCGGCTGGCCACGCTGCACAATCTCTACTA
CTACCAGCAGCTGATGGCCGGTCTGCGTGCGGCGATCCGCGACGGCGGCCTGGTGGGCTTTGTGGGCGATTTCTACGCGC
GGCGTGGGCAAACGCCGCCGACCCTATGA

Upstream 100 bases:

>100_bases
GCGAGTTGCCGCCGCCGGATCAGCGCTAGAGGAGGCAGCGGGTGCACGGCGACGGCCGTACCGCTATCCTGCACCGTTTT
GGGTGAGCGTGGAGGGTTCG

Downstream 100 bases:

>100_bases
CATAATACGCCGCATCCGGGCCGCCGGAGGGCGGTGTCAATGCGCTAACCGACAGATTCTGAAGGGGGAATGCGCGCATG
CTGATGGACTTCTTCATCTC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MQLDGAVTDTGALRFERMATDGAARRGRLHLPRGTIETPAFMPVGTYGTVKGLTPEELRELGAEIILGNTFHLWLRPGTE
VVRLHGDLHDFMNWQGPILTDSGGFQVYSLGELRKVTEEGVHFRSPVDGARVFMGPEESMAVQRELGSDIVMIFDECPPY
PAERSTVQRSMELSLRWAARSRQAHGDNPAALFGIVQGGVYPELRRASLQGLMDIGFDGYAIGGLSVGEPREERDAVLED
LEPRLPVDRPRYLMGVGKPEDLVECVRRGVDLFDCVIPTRNARNGFLFTSEGLVRLRNSRYRTDTAPVDPACDCYTCRNY
SRAYLKHLERCNEMLGARLATLHNLYYYQQLMAGLRAAIRDGGLVGFVGDFYARRGQTPPTL

Sequences:

>Translated_382_residues
MQLDGAVTDTGALRFERMATDGAARRGRLHLPRGTIETPAFMPVGTYGTVKGLTPEELRELGAEIILGNTFHLWLRPGTE
VVRLHGDLHDFMNWQGPILTDSGGFQVYSLGELRKVTEEGVHFRSPVDGARVFMGPEESMAVQRELGSDIVMIFDECPPY
PAERSTVQRSMELSLRWAARSRQAHGDNPAALFGIVQGGVYPELRRASLQGLMDIGFDGYAIGGLSVGEPREERDAVLED
LEPRLPVDRPRYLMGVGKPEDLVECVRRGVDLFDCVIPTRNARNGFLFTSEGLVRLRNSRYRTDTAPVDPACDCYTCRNY
SRAYLKHLERCNEMLGARLATLHNLYYYQQLMAGLRAAIRDGGLVGFVGDFYARRGQTPPTL
>Mature_382_residues
MQLDGAVTDTGALRFERMATDGAARRGRLHLPRGTIETPAFMPVGTYGTVKGLTPEELRELGAEIILGNTFHLWLRPGTE
VVRLHGDLHDFMNWQGPILTDSGGFQVYSLGELRKVTEEGVHFRSPVDGARVFMGPEESMAVQRELGSDIVMIFDECPPY
PAERSTVQRSMELSLRWAARSRQAHGDNPAALFGIVQGGVYPELRRASLQGLMDIGFDGYAIGGLSVGEPREERDAVLED
LEPRLPVDRPRYLMGVGKPEDLVECVRRGVDLFDCVIPTRNARNGFLFTSEGLVRLRNSRYRTDTAPVDPACDCYTCRNY
SRAYLKHLERCNEMLGARLATLHNLYYYQQLMAGLRAAIRDGGLVGFVGDFYARRGQTPPTL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=45.8452722063037, Blast_Score=305, Evalue=3e-83,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=66.9354838709677, Blast_Score=544, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=43.6111111111111, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25146502, Length=157, Percent_Identity=26.7515923566879, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920510, Length=359, Percent_Identity=44.0111420612813, Blast_Score=313, Evalue=9e-86,
Organism=Drosophila melanogaster, GI21355311, Length=221, Percent_Identity=23.9819004524887, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42585; Mature: 42585

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDGAVTDTGALRFERMATDGAARRGRLHLPRGTIETPAFMPVGTYGTVKGLTPEELRE
CCCCCCCCCCHHHHHHHHHCCCHHCCCEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHH
LGAEIILGNTFHLWLRPGTEVVRLHGDLHDFMNWQGPILTDSGGFQVYSLGELRKVTEEG
HCCCEEECCEEEEEECCCCEEEEECCCHHHHCCCCCCEEECCCCEEEEECHHHHHHHHCC
VHFRSPVDGARVFMGPEESMAVQRELGSDIVMIFDECPPYPAERSTVQRSMELSLRWAAR
CEECCCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
SRQAHGDNPAALFGIVQGGVYPELRRASLQGLMDIGFDGYAIGGLSVGEPREERDAVLED
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHH
LEPRLPVDRPRYLMGVGKPEDLVECVRRGVDLFDCVIPTRNARNGFLFTSEGLVRLRNSR
CCCCCCCCCCCCEEECCCHHHHHHHHHCCCCEEEHHCCCCCCCCCEEEECCCHHHHHCCC
YRTDTAPVDPACDCYTCRNYSRAYLKHLERCNEMLGARLATLHNLYYYQQLMAGLRAAIR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGGLVGFVGDFYARRGQTPPTL
CCCEEEHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQLDGAVTDTGALRFERMATDGAARRGRLHLPRGTIETPAFMPVGTYGTVKGLTPEELRE
CCCCCCCCCCHHHHHHHHHCCCHHCCCEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHH
LGAEIILGNTFHLWLRPGTEVVRLHGDLHDFMNWQGPILTDSGGFQVYSLGELRKVTEEG
HCCCEEECCEEEEEECCCCEEEEECCCHHHHCCCCCCEEECCCCEEEEECHHHHHHHHCC
VHFRSPVDGARVFMGPEESMAVQRELGSDIVMIFDECPPYPAERSTVQRSMELSLRWAAR
CEECCCCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
SRQAHGDNPAALFGIVQGGVYPELRRASLQGLMDIGFDGYAIGGLSVGEPREERDAVLED
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHH
LEPRLPVDRPRYLMGVGKPEDLVECVRRGVDLFDCVIPTRNARNGFLFTSEGLVRLRNSR
CCCCCCCCCCCCEEECCCHHHHHHHHHCCCCEEEHHCCCCCCCCCEEEECCCHHHHHCCC
YRTDTAPVDPACDCYTCRNYSRAYLKHLERCNEMLGARLATLHNLYYYQQLMAGLRAAIR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGGLVGFVGDFYARRGQTPPTL
CCCEEEHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA