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The map label for this gene is rpoS [H]

Identifier: 121998209

GI number: 121998209

Start: 1540818

End: 1541828

Strand: Reverse

Name: rpoS [H]

Synonym: Hhal_1427

Alternate gene names: 121998209

Gene position: 1541828-1540818 (Counterclockwise)

Preceding gene: 121998210

Following gene: 121998208

Centisome position: 57.56

GC content: 65.88

Gene sequence:

>1011_bases
ATGACCCTCGATACGGCGGTTGTCAGCGTGCACGCGTACGCTGATGTGGACGCAGAGCGCGATCCCCCGATGATCGATGA
CCCCGGCGAGCTGATCCAGCCCTACGGCATCGATGAGGGCAGCGAGCCCGAGCCGGCTCGCCGGCCCCGTAGGAGCCGAT
CCAGCACGGAGTGTCGCACCGCGCTGGACGCCACGCAGCTCTACCTGAATGAGATCGGCCACGCCTCCCTGCTCACGGCC
GAGGAGGAGGTCGCGCTGGCGAGACGCGTTCAGCAGGGCGATGCGGCAGCCCGGGCGCGCATGATCGAGAGCAACCTCCG
GCTGGTGGTCAAGATCGCCCGGCGCTACATGAACCGCGGCCTCGCCTTCCTGGACCTCATCGAAGAGGGCAACCTGGGGT
TGATCCGCGCCGTGGAGAAGTTTGATCCCGAGCGCGGGTTCCGTTTCTCGACCTATGCGACCTGGTGGATCCGGCAGACC
ATCGAGCGCGGCATCATGAATCAGACACGCACCATCCGGCTGCCGATCCACGTCATCAAAGAGATCAACCAGTACCTGCG
GACCCAGCGCCGCCTGACCCAGACACTGGATCACGAACCCACGGTCGATGAGATCGCCGATGCTATGGGGCGTTCGCCGG
AAGACGTCCGACGCATGCGCGGTCTCAACGAGGGGACCACCTCGGTGGATGTGCCGATCGGCAAGGATTCCGACCGGGTG
CTACTCGACGCCATTCCCGACGAGACGCAGGGCATGCCGGAGAATGTCCTCGAGGACGACGACGTCGTGCGGCATCTGCA
AGACTGGCTCGGCTTCCTCACTGACAAGCAGCGGGCCGTGCTGGAGCGGCGTTTCGGGCTCAACGGCCACGAGCGCTACA
CGCTCGAGCAGGTGGGCACGCAGGTGGGGGTGACCCGCGAGCGGGTACGACAGATCCAGATCGATGCACTGCGGCGGCTG
CGCGAGCTCATGGAGCGCGATGGCTACTCCCAGGAGGCGGTCTTCGGCTAG

Upstream 100 bases:

>100_bases
CCGCGCCGGGAGTTGACCAAGGAGCTGATCCTCCATCGGCTGTCCAAGGAAGCCGGGCGGGGAAGCCCGGCGAGCGGTTA
GAGACCACTGGGAGGTCCAT

Downstream 100 bases:

>100_bases
CGCGGGGCCCCGCGCAGGGATCAGGGCGCACCTACGGCAAAGGGTCGGGGATTTGGTAAGCTGACCCGGTAGCCGTAGGA
GGCGTCATGGCGAAGAAGCT

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTLDTAVVSVHAYADVDAERDPPMIDDPGELIQPYGIDEGSEPEPARRPRRSRSSTECRTALDATQLYLNEIGHASLLTA
EEEVALARRVQQGDAAARARMIESNLRLVVKIARRYMNRGLAFLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQT
IERGIMNQTRTIRLPIHVIKEINQYLRTQRRLTQTLDHEPTVDEIADAMGRSPEDVRRMRGLNEGTTSVDVPIGKDSDRV
LLDAIPDETQGMPENVLEDDDVVRHLQDWLGFLTDKQRAVLERRFGLNGHERYTLEQVGTQVGVTRERVRQIQIDALRRL
RELMERDGYSQEAVFG

Sequences:

>Translated_336_residues
MTLDTAVVSVHAYADVDAERDPPMIDDPGELIQPYGIDEGSEPEPARRPRRSRSSTECRTALDATQLYLNEIGHASLLTA
EEEVALARRVQQGDAAARARMIESNLRLVVKIARRYMNRGLAFLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQT
IERGIMNQTRTIRLPIHVIKEINQYLRTQRRLTQTLDHEPTVDEIADAMGRSPEDVRRMRGLNEGTTSVDVPIGKDSDRV
LLDAIPDETQGMPENVLEDDDVVRHLQDWLGFLTDKQRAVLERRFGLNGHERYTLEQVGTQVGVTRERVRQIQIDALRRL
RELMERDGYSQEAVFG
>Mature_335_residues
TLDTAVVSVHAYADVDAERDPPMIDDPGELIQPYGIDEGSEPEPARRPRRSRSSTECRTALDATQLYLNEIGHASLLTAE
EEVALARRVQQGDAAARARMIESNLRLVVKIARRYMNRGLAFLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTI
ERGIMNQTRTIRLPIHVIKEINQYLRTQRRLTQTLDHEPTVDEIADAMGRSPEDVRRMRGLNEGTTSVDVPIGKDSDRVL
LDAIPDETQGMPENVLEDDDVVRHLQDWLGFLTDKQRAVLERRFGLNGHERYTLEQVGTQVGVTRERVRQIQIDALRRLR
ELMERDGYSQEAVFG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=282, Percent_Identity=65.2482269503546, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1789448, Length=270, Percent_Identity=44.8148148148148, Blast_Score=221, Evalue=5e-59,
Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=28.6231884057971, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38460; Mature: 38329

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDTAVVSVHAYADVDAERDPPMIDDPGELIQPYGIDEGSEPEPARRPRRSRSSTECRT
CCCCHHHEEEEHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCHHCCCHHHHHH
ALDATQLYLNEIGHASLLTAEEEVALARRVQQGDAAARARMIESNLRLVVKIARRYMNRG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LAFLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERGIMNQTRTIRLPIHVIK
CHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
EINQYLRTQRRLTQTLDHEPTVDEIADAMGRSPEDVRRMRGLNEGTTSVDVPIGKDSDRV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCEEECCCCCCCCCE
LLDAIPDETQGMPENVLEDDDVVRHLQDWLGFLTDKQRAVLERRFGLNGHERYTLEQVGT
EEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
QVGVTRERVRQIQIDALRRLRELMERDGYSQEAVFG
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TLDTAVVSVHAYADVDAERDPPMIDDPGELIQPYGIDEGSEPEPARRPRRSRSSTECRT
CCCHHHEEEEHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCHHCCCHHHHHH
ALDATQLYLNEIGHASLLTAEEEVALARRVQQGDAAARARMIESNLRLVVKIARRYMNRG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LAFLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERGIMNQTRTIRLPIHVIK
CHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
EINQYLRTQRRLTQTLDHEPTVDEIADAMGRSPEDVRRMRGLNEGTTSVDVPIGKDSDRV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCEEECCCCCCCCCE
LLDAIPDETQGMPENVLEDDDVVRHLQDWLGFLTDKQRAVLERRFGLNGHERYTLEQVGT
EEECCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
QVGVTRERVRQIQIDALRRLRELMERDGYSQEAVFG
HHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]