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The map label for this gene is bigR [H]

Identifier: 121998207

GI number: 121998207

Start: 1539467

End: 1539826

Strand: Reverse

Name: bigR [H]

Synonym: Hhal_1425

Alternate gene names: 121998207

Gene position: 1539826-1539467 (Counterclockwise)

Preceding gene: 121998208

Following gene: 121998203

Centisome position: 57.49

GC content: 66.67

Gene sequence:

>360_bases
ATGTCGACGGAAACCGTGCAGCCAGAGATCCAGAGTAGCGCACCCGACGAGGGCGTGATACCGGACCCGACGGAGATGGA
CACGCGGGCCGCCGATGCCGCGGCCTTCCTCAAGGCCCTGGCCAACGAGCGGCGGTTGATGATCCTCTGCCATCTGGTCG
CCGGGGAGCGCTCGGTGGGTGAACTGGAACAGCTCCTCAAGATGCGCCAGCCGGCCCTGTCACAGCAGCTAGCGCGGCTG
CGCGAGGAGGGGATCGTGGCCACACGGCGCGAGTCTCGAACCATCTACTACCGCCTGGCTAGCCCCGAGACCGAGGCCCT
GCTGGAGCGCCTCTACGAACTCTTCTGTCAGCGCTCCTAG

Upstream 100 bases:

>100_bases
TGGATGACGATCCCGATAAGCAGGATCACCTGGGGTACTATTTCACTGTCATCGACGGCTGAGAGCCGCGTTGCATTCGC
GAAGCAGTCCGGAGCCGACC

Downstream 100 bases:

>100_bases
CGGGCCGGAGGCGGCGCCCCCCGGCGACCCCTGGCGGGTCTCGTGGGGGCGTCTACGGCTCAGAAGCGATAGCGGACTCG
GGCGTAGAGGTTGGAATCCC

Product: regulatory protein, ArsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 119; Mature: 118

Protein sequence:

>119_residues
MSTETVQPEIQSSAPDEGVIPDPTEMDTRAADAAAFLKALANERRLMILCHLVAGERSVGELEQLLKMRQPALSQQLARL
REEGIVATRRESRTIYYRLASPETEALLERLYELFCQRS

Sequences:

>Translated_119_residues
MSTETVQPEIQSSAPDEGVIPDPTEMDTRAADAAAFLKALANERRLMILCHLVAGERSVGELEQLLKMRQPALSQQLARL
REEGIVATRRESRTIYYRLASPETEALLERLYELFCQRS
>Mature_118_residues
STETVQPEIQSSAPDEGVIPDPTEMDTRAADAAAFLKALANERRLMILCHLVAGERSVGELEQLLKMRQPALSQQLARLR
EEGIVATRRESRTIYYRLASPETEALLERLYELFCQRS

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=93, Percent_Identity=34.4086021505376, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 13448; Mature: 13316

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTETVQPEIQSSAPDEGVIPDPTEMDTRAADAAAFLKALANERRLMILCHLVAGERSVG
CCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
ELEQLLKMRQPALSQQLARLREEGIVATRRESRTIYYRLASPETEALLERLYELFCQRS
HHHHHHHHHCHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STETVQPEIQSSAPDEGVIPDPTEMDTRAADAAAFLKALANERRLMILCHLVAGERSVG
CCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHH
ELEQLLKMRQPALSQQLARLREEGIVATRRESRTIYYRLASPETEALLERLYELFCQRS
HHHHHHHHHCHHHHHHHHHHHHCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]