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The map label for this gene is gltX

Identifier: 121998064

GI number: 121998064

Start: 1386996

End: 1388414

Strand: Reverse

Name: gltX

Synonym: Hhal_1274

Alternate gene names: 121998064

Gene position: 1388414-1386996 (Counterclockwise)

Preceding gene: 121998065

Following gene: 121998062

Centisome position: 51.84

GC content: 65.61

Gene sequence:

>1419_bases
ATGACCATGATCCGTACGCGTTTCGCGCCGAGCCCGACAGGTTACTTGCATATCGGCGGTGCCCGGACCGCTCTCTACTC
CTGGCTGCACACCCGCCGACACGGGGGGCGTTTCGTGCTGCGGATCGAGGACACGGATCGCGAGCGCTCCACGCCGGAGG
CGGTGAATGCGATCCTTGAGGGTATGGCCTGGCTGGGGCTCGATTACGACGAGGGCCCGTTCTATCAGACCGAGCGCTAT
GACCGGTATCGCCAGCACCTGCAGACGCTGCTCGACGCGGGGCAGGCGTACTACTGCTACTGCACCAAGGACCGCCTGGA
GCGTCTGCGTACTGAGCAGCAGGCGCGCAAGGAGAAGCCGCGCTACGACGGCCGTTGCCGCGATCTGGACGGGCCGCCCT
CCGAGGAGGTGGCCGACGAGCCGGTGATCCGTTTCCGCACCCCGCTCGAGGGGCATGTGGTGGTCGAGGACGCCATTCGG
GGCAAGGTCCAGTTCCTCAACTCGGAACTCGATGACCTGGTGATTGCGCGCGGCGACGGTTCGCCCACCTACAACTTCAC
GGTGGTGGTGGACGATCTGGAGATGGGCGTGACCGACGTCATCCGCGGTGACGACCACCTGAACAACACCCCCCGTCAGA
TCCACCTCTATCAGGCGCTCGGGTTCGAGCCGCCGCGCTTCGCGCACGTGCCCATGATCCTGGGCGAGGATGGCAAGCGC
CTGTCCAAGCGGCACGGGTCGGTCAGCGTGCTGCAGTACCGCGATGAGGGGTACCTGCCCGAGGCGCTGCTCAACTATCT
GGTGCGTCTCGGCTGGTCGCACGGCGATCAGGAAGTTTTCGGTGTCGACGAGCTCATTCAGCTCTTCGATATCAACGAAG
TGAACCACTCGGCCTCGACCTTCAACCCCTCCAAGCTGCAGTGGCTCAATCAGCAGCACATCATGCGCGCCGAGCCCAAC
CACATCGCCCGGCATCTGGGGCCGTTCCTGGCTGAGCGGGGCGTCGATCCAGCCGAAGGTCCGGCGCTGGAGGCGGTCGT
GCGCACCCAGCAGGAGCGGGCCAAGACGTTGGTGGAGATGGCCGACAACAGCCTGTTCTTCTACCGTCGGCCGGAGGCCT
ACGAGGAGAAGGCGGCGCGCAAGAACTTCAAGGAGGGCACCGCCGAGATTCTCGAGCACTGCCAGCACTGCTTCTCGGGG
CTGCCGAGCTGGGATGCGGAGTCGATCCATGGGGTTGTCACCGAGGCCGCTGAGGCCTTCGATGTGAAGATGGGCAAGGT
GGCACAGCCGCTGCGGGTGGCTGTTTCGGGGAGTGCGGTCTCGCCGCCGATCGACGCCACGCTGGAGCTGCTTGGTCGTG
AGGAGACGGTGGCCCGGGTGGGCCAGGCCGCCGAGTGGGTCCGGCAGAACGTTGGTTAA

Upstream 100 bases:

>100_bases
TCTTAACGGCCCCGTGCCCTCGCGTCAAATGGGGCGCGTCGGCGGTGTCGCTTTTGATAGCATACCGGCCTTCAGCACGG
GGAGAAGGAAAAAGGCCACC

Downstream 100 bases:

>100_bases
TCGAAGTCGGGGAGGGGTGTTGACGACCCCGAAAGCCATCCCTAGAATACGCACACATAACGCAACGGGGCCATAGCTCA
GCTGGGAGAGCGCCTGCATG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTMIRTRFAPSPTGYLHIGGARTALYSWLHTRRHGGRFVLRIEDTDRERSTPEAVNAILEGMAWLGLDYDEGPFYQTERY
DRYRQHLQTLLDAGQAYYCYCTKDRLERLRTEQQARKEKPRYDGRCRDLDGPPSEEVADEPVIRFRTPLEGHVVVEDAIR
GKVQFLNSELDDLVIARGDGSPTYNFTVVVDDLEMGVTDVIRGDDHLNNTPRQIHLYQALGFEPPRFAHVPMILGEDGKR
LSKRHGSVSVLQYRDEGYLPEALLNYLVRLGWSHGDQEVFGVDELIQLFDINEVNHSASTFNPSKLQWLNQQHIMRAEPN
HIARHLGPFLAERGVDPAEGPALEAVVRTQQERAKTLVEMADNSLFFYRRPEAYEEKAARKNFKEGTAEILEHCQHCFSG
LPSWDAESIHGVVTEAAEAFDVKMGKVAQPLRVAVSGSAVSPPIDATLELLGREETVARVGQAAEWVRQNVG

Sequences:

>Translated_472_residues
MTMIRTRFAPSPTGYLHIGGARTALYSWLHTRRHGGRFVLRIEDTDRERSTPEAVNAILEGMAWLGLDYDEGPFYQTERY
DRYRQHLQTLLDAGQAYYCYCTKDRLERLRTEQQARKEKPRYDGRCRDLDGPPSEEVADEPVIRFRTPLEGHVVVEDAIR
GKVQFLNSELDDLVIARGDGSPTYNFTVVVDDLEMGVTDVIRGDDHLNNTPRQIHLYQALGFEPPRFAHVPMILGEDGKR
LSKRHGSVSVLQYRDEGYLPEALLNYLVRLGWSHGDQEVFGVDELIQLFDINEVNHSASTFNPSKLQWLNQQHIMRAEPN
HIARHLGPFLAERGVDPAEGPALEAVVRTQQERAKTLVEMADNSLFFYRRPEAYEEKAARKNFKEGTAEILEHCQHCFSG
LPSWDAESIHGVVTEAAEAFDVKMGKVAQPLRVAVSGSAVSPPIDATLELLGREETVARVGQAAEWVRQNVG
>Mature_471_residues
TMIRTRFAPSPTGYLHIGGARTALYSWLHTRRHGGRFVLRIEDTDRERSTPEAVNAILEGMAWLGLDYDEGPFYQTERYD
RYRQHLQTLLDAGQAYYCYCTKDRLERLRTEQQARKEKPRYDGRCRDLDGPPSEEVADEPVIRFRTPLEGHVVVEDAIRG
KVQFLNSELDDLVIARGDGSPTYNFTVVVDDLEMGVTDVIRGDDHLNNTPRQIHLYQALGFEPPRFAHVPMILGEDGKRL
SKRHGSVSVLQYRDEGYLPEALLNYLVRLGWSHGDQEVFGVDELIQLFDINEVNHSASTFNPSKLQWLNQQHIMRAEPNH
IARHLGPFLAERGVDPAEGPALEAVVRTQQERAKTLVEMADNSLFFYRRPEAYEEKAARKNFKEGTAEILEHCQHCFSGL
PSWDAESIHGVVTEAAEAFDVKMGKVAQPLRVAVSGSAVSPPIDATLELLGREETVARVGQAAEWVRQNVG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=32.4489795918367, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI1788743, Length=465, Percent_Identity=54.1935483870968, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI226510925, Length=243, Percent_Identity=37.8600823045267, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786895, Length=123, Percent_Identity=30.0813008130081, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=30.6079664570231, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324540, Length=506, Percent_Identity=31.4229249011858, Blast_Score=253, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6324742, Length=211, Percent_Identity=28.9099526066351, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=32.9268292682927, Blast_Score=270, Evalue=2e-72,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_HALHL (A1WWI7)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002851.1
- ProteinModelPortal:   A1WWI7
- SMR:   A1WWI7
- STRING:   A1WWI7
- GeneID:   4709395
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1274
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   A1WWI7
- ProtClustDB:   PRK01406
- BioCyc:   HHAL349124:HHAL_1274-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53491; Mature: 53360

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMIRTRFAPSPTGYLHIGGARTALYSWLHTRRHGGRFVLRIEDTDRERSTPEAVNAILE
CCCEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
GMAWLGLDYDEGPFYQTERYDRYRQHLQTLLDAGQAYYCYCTKDRLERLRTEQQARKEKP
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCC
RYDGRCRDLDGPPSEEVADEPVIRFRTPLEGHVVVEDAIRGKVQFLNSELDDLVIARGDG
CCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCEEEHHHHHHHHHHHHCCCCCEEEECCCC
SPTYNFTVVVDDLEMGVTDVIRGDDHLNNTPRQIHLYQALGFEPPRFAHVPMILGEDGKR
CCCEEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEEHHHCCCCCCCEECCEEECCCCHH
LSKRHGSVSVLQYRDEGYLPEALLNYLVRLGWSHGDQEVFGVDELIQLFDINEVNHSAST
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHCHHHHHHHHCCCCCCCCCCC
FNPSKLQWLNQQHIMRAEPNHIARHLGPFLAERGVDPAEGPALEAVVRTQQERAKTLVEM
CCHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ADNSLFFYRRPEAYEEKAARKNFKEGTAEILEHCQHCFSGLPSWDAESIHGVVTEAAEAF
HCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DVKMGKVAQPLRVAVSGSAVSPPIDATLELLGREETVARVGQAAEWVRQNVG
HHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMIRTRFAPSPTGYLHIGGARTALYSWLHTRRHGGRFVLRIEDTDRERSTPEAVNAILE
CCEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHH
GMAWLGLDYDEGPFYQTERYDRYRQHLQTLLDAGQAYYCYCTKDRLERLRTEQQARKEKP
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHCCC
RYDGRCRDLDGPPSEEVADEPVIRFRTPLEGHVVVEDAIRGKVQFLNSELDDLVIARGDG
CCCCCCCCCCCCCCHHHCCCCCEEECCCCCCCEEEHHHHHHHHHHHHCCCCCEEEECCCC
SPTYNFTVVVDDLEMGVTDVIRGDDHLNNTPRQIHLYQALGFEPPRFAHVPMILGEDGKR
CCCEEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEEHHHCCCCCCCEECCEEECCCCHH
LSKRHGSVSVLQYRDEGYLPEALLNYLVRLGWSHGDQEVFGVDELIQLFDINEVNHSAST
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHCHHHHHHHHCCCCCCCCCCC
FNPSKLQWLNQQHIMRAEPNHIARHLGPFLAERGVDPAEGPALEAVVRTQQERAKTLVEM
CCHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ADNSLFFYRRPEAYEEKAARKNFKEGTAEILEHCQHCFSGLPSWDAESIHGVVTEAAEAF
HCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DVKMGKVAQPLRVAVSGSAVSPPIDATLELLGREETVARVGQAAEWVRQNVG
HHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA