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The map label for this gene is glnA [H]

Identifier: 121998007

GI number: 121998007

Start: 1321402

End: 1322817

Strand: Reverse

Name: glnA [H]

Synonym: Hhal_1217

Alternate gene names: 121998007

Gene position: 1322817-1321402 (Counterclockwise)

Preceding gene: 121998008

Following gene: 121998006

Centisome position: 49.39

GC content: 64.76

Gene sequence:

>1416_bases
ATGGCGCGTACCGGAGAAGATGTCCTCAAGCTGATCGAGGACGAAGACGTCAAATTCGTCGACCTTCGCTTTACTGACAC
CCGCGGCAAGGAGATGCACGTGACCTTGCCGGCCTCCCAGGTCGACGAGGATCTGTTCGAACACGGCTCCATGTTCGACG
GCTCCTCGATCCAGGGCTGGAAGGGGATCCAGGAGTCGGACATGATCATGCTCCCCGATCCCGACTCCGCCACCCTCGAC
CCCTTCTACGATGAACCCACCCTGAATCTGGTCTGCGACATCCTCGAGCCGAGCACCCTGCAGGGCTACGACCGGGATCC
GCGTTCGGTGGCCCGGCGCGCCGAGGCATACCTGGCCTCCACCGGCGTGGGTGACACCGCCCTGTTCGGTCCCGAGCCGG
AGTTCTTCGTCTTCGACGACGTCCGCTGGGGCGCCGACATGAGCGGGACCTTCTACAAGATCGACTCCGAAGAGGCCGGC
TGGAACTCCGAGCGGGTCTACAAAGACGGCAACATCGGCCACCGCCCCGGTGTGAAGGGCGGCTACTTCCCGGTACCGCC
GGTAGACTCGCTGCACGACATCCGTGCCGCCATGTGCCAGGCGATGGAGGAGATGGGCCTGGAGCCGGAGGTCCACCACC
ACGAGGTGGCGACCGCTGGGCAGTGCGAGATCGGCGTGGCTGCTCGCACCCTGGTCAAGAAGGCCGACGAGCTGCAGACG
CTCAAGTACTGCGTGCACAACGTCGCCCACGCCTACGGCAAGACCGCCACCTTCATGCCCAAGCCGCTGGTGGGCGACAA
CGGCAGCGGCATGCACGTGCACCAGTCGGTCTCCAAGGGCGGTAACAACATCTTCGCCGGGGACCAGTACGGCGGGCTCT
CCGAGGAGGCGCTCTACTACATCGGCGGCATCATCAAGCACGCCAAGGCGATCAACGCCTTCGCCAACGCCTCCACCAAC
AGCTACAAGCGCCTGGTGCCCGGCTTCGAGGCGCCGGTGCTGCTCGCCTACTCGGCGCGCAATCGCTCCGCCTCGGTGCG
GGTGCCCTATGTGGCCAACCCCAAGGCGCGGCGGATCGAGGTCCGCTTCCCGGATCCCACAGGCAACCCGTACCTGACCT
TCGCCGCCATGCTGATGGCCGGTCTCGATGGCATCCGCAACAAGATCCACCCCGGCGAGGCGATGGACAAGGACCTCTAC
GACCTGCCGCCGGAGGAGGAGAAGGAGATCCCCAAGGTCGCCTTCCAGCTCGACGAGGCGCTCGAGGCCCTGGACAACGA
CCGCGAGTTCCTCAAGCAGGGCGGAGTCTTCTCCGACGACCTGATTGACGCGTACATCCAATTGAAGATGGACGACGTCA
CGCGCCTGCGCATGACCACCCATCCGGTGGAGTTCGACATGTACTACAGCGTCTAA

Upstream 100 bases:

>100_bases
TGGGCCGCGAAATGCCCCACAATAAGTCGCGGCTGCGGGGCTTTGCACCTTCCCGGCGCACGCTCCGCCCAATTGATTCC
TTTGCAGAGGCTGTGAGCTT

Downstream 100 bases:

>100_bases
ACCCTTCGGGGCGGATGCTGTGTACAGGGGGCGGGGCCGGCTGGCTGCCGCCCCTTTTTATTGCATTGCACCCGGATGGT
GCAGTCACCGTACGCCTGCG

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MARTGEDVLKLIEDEDVKFVDLRFTDTRGKEMHVTLPASQVDEDLFEHGSMFDGSSIQGWKGIQESDMIMLPDPDSATLD
PFYDEPTLNLVCDILEPSTLQGYDRDPRSVARRAEAYLASTGVGDTALFGPEPEFFVFDDVRWGADMSGTFYKIDSEEAG
WNSERVYKDGNIGHRPGVKGGYFPVPPVDSLHDIRAAMCQAMEEMGLEPEVHHHEVATAGQCEIGVAARTLVKKADELQT
LKYCVHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSVSKGGNNIFAGDQYGGLSEEALYYIGGIIKHAKAINAFANASTN
SYKRLVPGFEAPVLLAYSARNRSASVRVPYVANPKARRIEVRFPDPTGNPYLTFAAMLMAGLDGIRNKIHPGEAMDKDLY
DLPPEEEKEIPKVAFQLDEALEALDNDREFLKQGGVFSDDLIDAYIQLKMDDVTRLRMTTHPVEFDMYYSV

Sequences:

>Translated_471_residues
MARTGEDVLKLIEDEDVKFVDLRFTDTRGKEMHVTLPASQVDEDLFEHGSMFDGSSIQGWKGIQESDMIMLPDPDSATLD
PFYDEPTLNLVCDILEPSTLQGYDRDPRSVARRAEAYLASTGVGDTALFGPEPEFFVFDDVRWGADMSGTFYKIDSEEAG
WNSERVYKDGNIGHRPGVKGGYFPVPPVDSLHDIRAAMCQAMEEMGLEPEVHHHEVATAGQCEIGVAARTLVKKADELQT
LKYCVHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSVSKGGNNIFAGDQYGGLSEEALYYIGGIIKHAKAINAFANASTN
SYKRLVPGFEAPVLLAYSARNRSASVRVPYVANPKARRIEVRFPDPTGNPYLTFAAMLMAGLDGIRNKIHPGEAMDKDLY
DLPPEEEKEIPKVAFQLDEALEALDNDREFLKQGGVFSDDLIDAYIQLKMDDVTRLRMTTHPVEFDMYYSV
>Mature_470_residues
ARTGEDVLKLIEDEDVKFVDLRFTDTRGKEMHVTLPASQVDEDLFEHGSMFDGSSIQGWKGIQESDMIMLPDPDSATLDP
FYDEPTLNLVCDILEPSTLQGYDRDPRSVARRAEAYLASTGVGDTALFGPEPEFFVFDDVRWGADMSGTFYKIDSEEAGW
NSERVYKDGNIGHRPGVKGGYFPVPPVDSLHDIRAAMCQAMEEMGLEPEVHHHEVATAGQCEIGVAARTLVKKADELQTL
KYCVHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSVSKGGNNIFAGDQYGGLSEEALYYIGGIIKHAKAINAFANASTNS
YKRLVPGFEAPVLLAYSARNRSASVRVPYVANPKARRIEVRFPDPTGNPYLTFAAMLMAGLDGIRNKIHPGEAMDKDLYD
LPPEEEKEIPKVAFQLDEALEALDNDREFLKQGGVFSDDLIDAYIQLKMDDVTRLRMTTHPVEFDMYYSV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=237, Percent_Identity=26.1603375527426, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=69.6581196581197, Blast_Score=705, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=394, Percent_Identity=25.8883248730964, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52208; Mature: 52077

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTGEDVLKLIEDEDVKFVDLRFTDTRGKEMHVTLPASQVDEDLFEHGSMFDGSSIQGW
CCCCHHHHHHHHCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCC
KGIQESDMIMLPDPDSATLDPFYDEPTLNLVCDILEPSTLQGYDRDPRSVARRAEAYLAS
CCCCCCCEEEECCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHH
TGVGDTALFGPEPEFFVFDDVRWGADMSGTFYKIDSEEAGWNSERVYKDGNIGHRPGVKG
CCCCCCEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
GYFPVPPVDSLHDIRAAMCQAMEEMGLEPEVHHHEVATAGQCEIGVAARTLVKKADELQT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LKYCVHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSVSKGGNNIFAGDQYGGLSEEALYY
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHCCCCEEECCCCCCCCCHHHHHH
IGGIIKHAKAINAFANASTNSYKRLVPGFEAPVLLAYSARNRSASVRVPYVANPKARRIE
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEECEECCCCCEEEE
VRFPDPTGNPYLTFAAMLMAGLDGIRNKIHPGEAMDKDLYDLPPEEEKEIPKVAFQLDEA
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHCCCCCHHHHHHHHHHHHHHH
LEALDNDREFLKQGGVFSDDLIDAYIQLKMDDVTRLRMTTHPVEFDMYYSV
HHHHCCCHHHHHHCCCCCHHHHHHHHEEEECCCEEEEEEECCEEEEEEECC
>Mature Secondary Structure 
ARTGEDVLKLIEDEDVKFVDLRFTDTRGKEMHVTLPASQVDEDLFEHGSMFDGSSIQGW
CCCHHHHHHHHCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCC
KGIQESDMIMLPDPDSATLDPFYDEPTLNLVCDILEPSTLQGYDRDPRSVARRAEAYLAS
CCCCCCCEEEECCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHH
TGVGDTALFGPEPEFFVFDDVRWGADMSGTFYKIDSEEAGWNSERVYKDGNIGHRPGVKG
CCCCCCEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
GYFPVPPVDSLHDIRAAMCQAMEEMGLEPEVHHHEVATAGQCEIGVAARTLVKKADELQT
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LKYCVHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSVSKGGNNIFAGDQYGGLSEEALYY
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHCCCCEEECCCCCCCCCHHHHHH
IGGIIKHAKAINAFANASTNSYKRLVPGFEAPVLLAYSARNRSASVRVPYVANPKARRIE
HHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEECCCCCCEEEECEECCCCCEEEE
VRFPDPTGNPYLTFAAMLMAGLDGIRNKIHPGEAMDKDLYDLPPEEEKEIPKVAFQLDEA
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHCCCCCHHHHHHHHHHHHHHH
LEALDNDREFLKQGGVFSDDLIDAYIQLKMDDVTRLRMTTHPVEFDMYYSV
HHHHCCCHHHHHHCCCCCHHHHHHHHEEEECCCEEEEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]