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The map label for this gene is sucB [H]

Identifier: 121997876

GI number: 121997876

Start: 1178835

End: 1180124

Strand: Reverse

Name: sucB [H]

Synonym: Hhal_1085

Alternate gene names: 121997876

Gene position: 1180124-1178835 (Counterclockwise)

Preceding gene: 121997877

Following gene: 121997875

Centisome position: 44.06

GC content: 70.16

Gene sequence:

>1290_bases
ATGAGCGTCGAGATCAAGGTTCCAGCGCTGCCCGAGTCCGTCAGTGAGGCGACAGTGGTGGCCTGGCACAAACAGCCCGG
GGAGGCGGTGACCCAGGACCAGAACCTGGTCGACCTGGAGACCGATAAGGTTGTCCTGGAGGTCCCGGCCCCCGCCGACG
GTGTGCTGGAGGCGATCGACCGGGCCGTGGGCGATACGGTGACGCCGGACGACGTCCTCGGCCGTGTCGAGGCGGGTGCG
GCCCCGGCGGCCGAGGTCGCCTCGGCGGCAGCCGCGGGCGGACAGGCGTCGGCGGAGTCGGCCCCGGGGAGCGCCGGTGA
CGCGGCCTCCGGCGCGGCCCCGCCGGCGGCGGAGGCCCCGGCTCCAGCAGCCAGCCTGGATGGTCTGAGCCCGGCGGTGC
GCCGACTGGTCCGCGAGCACGGGCTCGATCCGGCGCAGATCGAATCGTCCAGTGGGGACGGGCGCCTGACCAAGGAGGAC
GTCCTGCGCCACCTGGAGCGTCGCGCCGAGGCCGAGGCGGCGCCCACAGCGGCTACAGCGCCGCAGAGCGCTCCCGCCGA
GGAGTCCGCCGCAGCCGCCCCCCTGGGGGGGCGGCCCGAGCAGCGGGTACCGATGACCCGCCTGCGCCAGCGCATCGCCG
AGCGGCTGGTCGAGGCGCAGCAGAACGCGGCCATGCTCACCACCTTCAACGAGGTGAACATGCAGCCGGTGATGGATCTG
CGCGCCCGTTACCGCGAGCGCTTCGAAAAGGCCCACGACGTGCGCCTGGGTTTTATGTCGTTCTTCGTCAAGGCGGCGGT
GGAGGCCCTCAAGCGCTACCCGGCGGTCAATGCCTCCATCGATGGGCAGGACATCATCTACCACGGGTATTACGACATCG
GCATCGCCGTCTCCTCGCCGCGCGGCCTGGTGGTGCCGGTGCTCCGCGATGCCGATCGCCTCTCCTTCGCCGAGATCGAG
GCGCAGATCAACGAGCTCGGCAAGCGCGCCCAGCAGGGCAAGCTGAGCATGGACGAGCTCACCGGCGGGACCTTCACCGT
TACCAACGGCGGCATCTTCGGCTCGCTGCTCTCCACGCCGATCATCAACCCGCCGCAGAGCGGCATCCTGGGGATGCACA
AGATCCAGGAACGGCCGGTGGTCGAAGACGGTGAGATCGTGGTCCGGCCGATGATGTACCTGGCCCACACCTACGACCAT
CGCCTCATCGACGGCCGGGAAGCCGTGCAGTTCCTGGTGACCATCAAGGACTGCATCGAGGATCCCGCCCGCCTGCTGCT
CGAGGTCTAG

Upstream 100 bases:

>100_bases
GCGCCACCACGATCAGCAGCGGGCGCTGGTCGAGGACGCGCTCGCCTAGCCGGCACGTGGCAACGGAGTCCGCACGAGAA
CACCAGAGAAGGGGACCTGC

Downstream 100 bases:

>100_bases
CGGCGCAGGCCCCTCGTGGGCCGGATGCGTCCGAGCGCACACACAACCACAGAACAGGGTCGCAACATGTCCAATCACTA
CGATGTCATCGTCATCGGTG

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSVEIKVPALPESVSEATVVAWHKQPGEAVTQDQNLVDLETDKVVLEVPAPADGVLEAIDRAVGDTVTPDDVLGRVEAGA
APAAEVASAAAAGGQASAESAPGSAGDAASGAAPPAAEAPAPAASLDGLSPAVRRLVREHGLDPAQIESSSGDGRLTKED
VLRHLERRAEAEAAPTAATAPQSAPAEESAAAAPLGGRPEQRVPMTRLRQRIAERLVEAQQNAAMLTTFNEVNMQPVMDL
RARYRERFEKAHDVRLGFMSFFVKAAVEALKRYPAVNASIDGQDIIYHGYYDIGIAVSSPRGLVVPVLRDADRLSFAEIE
AQINELGKRAQQGKLSMDELTGGTFTVTNGGIFGSLLSTPIINPPQSGILGMHKIQERPVVEDGEIVVRPMMYLAHTYDH
RLIDGREAVQFLVTIKDCIEDPARLLLEV

Sequences:

>Translated_429_residues
MSVEIKVPALPESVSEATVVAWHKQPGEAVTQDQNLVDLETDKVVLEVPAPADGVLEAIDRAVGDTVTPDDVLGRVEAGA
APAAEVASAAAAGGQASAESAPGSAGDAASGAAPPAAEAPAPAASLDGLSPAVRRLVREHGLDPAQIESSSGDGRLTKED
VLRHLERRAEAEAAPTAATAPQSAPAEESAAAAPLGGRPEQRVPMTRLRQRIAERLVEAQQNAAMLTTFNEVNMQPVMDL
RARYRERFEKAHDVRLGFMSFFVKAAVEALKRYPAVNASIDGQDIIYHGYYDIGIAVSSPRGLVVPVLRDADRLSFAEIE
AQINELGKRAQQGKLSMDELTGGTFTVTNGGIFGSLLSTPIINPPQSGILGMHKIQERPVVEDGEIVVRPMMYLAHTYDH
RLIDGREAVQFLVTIKDCIEDPARLLLEV
>Mature_428_residues
SVEIKVPALPESVSEATVVAWHKQPGEAVTQDQNLVDLETDKVVLEVPAPADGVLEAIDRAVGDTVTPDDVLGRVEAGAA
PAAEVASAAAAGGQASAESAPGSAGDAASGAAPPAAEAPAPAASLDGLSPAVRRLVREHGLDPAQIESSSGDGRLTKEDV
LRHLERRAEAEAAPTAATAPQSAPAEESAAAAPLGGRPEQRVPMTRLRQRIAERLVEAQQNAAMLTTFNEVNMQPVMDLR
ARYRERFEKAHDVRLGFMSFFVKAAVEALKRYPAVNASIDGQDIIYHGYYDIGIAVSSPRGLVVPVLRDADRLSFAEIEA
QINELGKRAQQGKLSMDELTGGTFTVTNGGIFGSLLSTPIINPPQSGILGMHKIQERPVVEDGEIVVRPMMYLAHTYDHR
LIDGREAVQFLVTIKDCIEDPARLLLEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=436, Percent_Identity=26.3761467889908, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=26.4770240700219, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=26.4770240700219, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI31711992, Length=443, Percent_Identity=28.6681715575621, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=33.5403726708075, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786946, Length=430, Percent_Identity=55.8139534883721, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1786305, Length=435, Percent_Identity=31.264367816092, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=429, Percent_Identity=39.6270396270396, Blast_Score=291, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI17537937, Length=437, Percent_Identity=26.5446224256293, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=450, Percent_Identity=28.4444444444444, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=29.6774193548387, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=40.7494145199063, Blast_Score=314, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6324258, Length=450, Percent_Identity=26.6666666666667, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=55.0218340611354, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI18859875, Length=441, Percent_Identity=27.891156462585, Blast_Score=164, Evalue=9e-41,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=29.3859649122807, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=29.3859649122807, Blast_Score=115, Evalue=8e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45569; Mature: 45438

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEIKVPALPESVSEATVVAWHKQPGEAVTQDQNLVDLETDKVVLEVPAPADGVLEAID
CCEEEECCCCCCCCCCCEEEEEECCCCCHHCCCCCEEEECCCEEEEECCCCCHHHHHHHH
RAVGDTVTPDDVLGRVEAGAAPAAEVASAAAAGGQASAESAPGSAGDAASGAAPPAAEAP
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APAASLDGLSPAVRRLVREHGLDPAQIESSSGDGRLTKEDVLRHLERRAEAEAAPTAATA
CCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
PQSAPAEESAAAAPLGGRPEQRVPMTRLRQRIAERLVEAQQNAAMLTTFNEVNMQPVMDL
CCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHH
RARYRERFEKAHDVRLGFMSFFVKAAVEALKRYPAVNASIDGQDIIYHGYYDIGIAVSSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECC
RGLVVPVLRDADRLSFAEIEAQINELGKRAQQGKLSMDELTGGTFTVTNGGIFGSLLSTP
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCHHHHHHHCC
IINPPQSGILGMHKIQERPVVEDGEIVVRPMMYLAHTYDHRLIDGREAVQFLVTIKDCIE
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
DPARLLLEV
CHHHHHHCC
>Mature Secondary Structure 
SVEIKVPALPESVSEATVVAWHKQPGEAVTQDQNLVDLETDKVVLEVPAPADGVLEAID
CEEEECCCCCCCCCCCEEEEEECCCCCHHCCCCCEEEECCCEEEEECCCCCHHHHHHHH
RAVGDTVTPDDVLGRVEAGAAPAAEVASAAAAGGQASAESAPGSAGDAASGAAPPAAEAP
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APAASLDGLSPAVRRLVREHGLDPAQIESSSGDGRLTKEDVLRHLERRAEAEAAPTAATA
CCCCCCCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
PQSAPAEESAAAAPLGGRPEQRVPMTRLRQRIAERLVEAQQNAAMLTTFNEVNMQPVMDL
CCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCHHHHHH
RARYRERFEKAHDVRLGFMSFFVKAAVEALKRYPAVNASIDGQDIIYHGYYDIGIAVSSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECC
RGLVVPVLRDADRLSFAEIEAQINELGKRAQQGKLSMDELTGGTFTVTNGGIFGSLLSTP
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCHHHHHHHCC
IINPPQSGILGMHKIQERPVVEDGEIVVRPMMYLAHTYDHRLIDGREAVQFLVTIKDCIE
CCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
DPARLLLEV
CHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]