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The map label for this gene is lpdG [H]

Identifier: 121997875

GI number: 121997875

Start: 1177347

End: 1178768

Strand: Reverse

Name: lpdG [H]

Synonym: Hhal_1084

Alternate gene names: 121997875

Gene position: 1178768-1177347 (Counterclockwise)

Preceding gene: 121997876

Following gene: 121997874

Centisome position: 44.01

GC content: 67.51

Gene sequence:

>1422_bases
ATGTCCAATCACTACGATGTCATCGTCATCGGTGCCGGTCCCGCCGGCTACAGCGCTGCCATCCGCTGCGCCCAGCTGGG
CATGCGCACCGCCGTGGTCGACGCCTTCGTCTTCAAGGACGGCGAACCCGCCCTGGGCGGGACCTGCCTGAACGTCGGCT
GCATCCCCTCCAAGGCGCTGCTCGACAGCTCCGAGCAGTACCACAAGGTCACCCACCAGCTCAGCGCCCACGGCATCACG
GCCCAGGGGGTGGAGCTGGATGTCGAGAAGATGATCGCCCGCAAGGATCGGGTGGTGAAGGACCTGACCGGCGGCATCCG
CCAGCTGTTCAAGGCCAACAAGATCGAGTGGCTGCACGGCCACGGTCAGCTGGTCGAGGCGAAGACCGTGGAGGTGCGCC
GTCCGGATGGCAGCACCGAGCGGCACAGCGCTGACTCCGTGGTGCTGGCCACGGGCTCGCAGAGTGTCGAGCTCGGCGCC
GCACCACTGGATCACGAACGCATCGTCAACTCCGATCGGGCCCTGGACTTCACCGAGGTCCCGGAGCGACTCGGTATCAT
CGGCGCCGGGGTCATCGGCCTGGAGATGGGGTCGGTGTGGAGCCGGCTTGGTTCCGAGGTCGTGCTGCTCGAGGCGCAGG
ACGAGTTCCTCGGGCCGGTGGACCGCCAGGTGGCGCGCACCGCGCAGAAGGAGTTCAAGAAGCAGGGCCTGGACATCCGC
TTGGGCTGCCGGGTGACCGGCACCCGGGTCGATGGCTCGGTCACGGTCTCCTACGAGGACAAGAACGGGAAGCAGGAGCT
GGAGGTCGATCGCCTCATCGTCTGCGTCGGCCGTCGCCCGAACACCGACGACCTGTGCTCCAAGGAGGTGGACCTGCTCC
TCGATGAGCGCGGCTTCATCAACGTCGACGAGGACTGCAAGACCAACCTGCCGGACGTCTACGCCGTCGGTGACGTGGTC
CGCGGCCCGATGCTCGCCCACAAGGGCCAGGAAGAGGGGATCGTGGTGGCCGAACGGATCGCCGGTAAGGGGGGGCACCT
CAACTACGAGACCATCCCCTGGGTCATCTACACCGACCCCGAGATCGCCTGGGTGGGCCGGACCGAGGAGCAGCTGAAGA
AGGCCGGCATCCCCTACAACACCGGGGTTTTCGGCTTCGCTGCCAATGGTCGGGCCCGCGCCATGGATCAGACGGCGGGG
ATGGTCAAGCTCATCGCCCACGCCGAGACCGACCGGCTGCTCGGGGCCCATATCATCGGCCCGCAGGCCTCCGAGCTCAT
TGCCGAGGCCGTGGTGACCATGGAGTTCGCCGGTTCCGCCGAGGACATCGCCCGGACCATCCACGCCCACCCGACGCTCT
CCGAGACGGTCCACGAGGCGGCCCTGGCCGTCGGCAAGCGGGCGATCCACAAGGCCAACTGA

Upstream 100 bases:

>100_bases
CGAGGATCCCGCCCGCCTGCTGCTCGAGGTCTAGCGGCGCAGGCCCCTCGTGGGCCGGATGCGTCCGAGCGCACACACAA
CCACAGAACAGGGTCGCAAC

Downstream 100 bases:

>100_bases
GCCGGCTCAGGCCCCCGCGCCGCCCCCGCCCGGGGGCGGCCGCCGGTCGGGTCAGCGCCAGCGGCCCCGCTGGGCGACAG
TTTTGGGGTATTCTGGAGCC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSNHYDVIVIGAGPAGYSAAIRCAQLGMRTAVVDAFVFKDGEPALGGTCLNVGCIPSKALLDSSEQYHKVTHQLSAHGIT
AQGVELDVEKMIARKDRVVKDLTGGIRQLFKANKIEWLHGHGQLVEAKTVEVRRPDGSTERHSADSVVLATGSQSVELGA
APLDHERIVNSDRALDFTEVPERLGIIGAGVIGLEMGSVWSRLGSEVVLLEAQDEFLGPVDRQVARTAQKEFKKQGLDIR
LGCRVTGTRVDGSVTVSYEDKNGKQELEVDRLIVCVGRRPNTDDLCSKEVDLLLDERGFINVDEDCKTNLPDVYAVGDVV
RGPMLAHKGQEEGIVVAERIAGKGGHLNYETIPWVIYTDPEIAWVGRTEEQLKKAGIPYNTGVFGFAANGRARAMDQTAG
MVKLIAHAETDRLLGAHIIGPQASELIAEAVVTMEFAGSAEDIARTIHAHPTLSETVHEAALAVGKRAIHKAN

Sequences:

>Translated_473_residues
MSNHYDVIVIGAGPAGYSAAIRCAQLGMRTAVVDAFVFKDGEPALGGTCLNVGCIPSKALLDSSEQYHKVTHQLSAHGIT
AQGVELDVEKMIARKDRVVKDLTGGIRQLFKANKIEWLHGHGQLVEAKTVEVRRPDGSTERHSADSVVLATGSQSVELGA
APLDHERIVNSDRALDFTEVPERLGIIGAGVIGLEMGSVWSRLGSEVVLLEAQDEFLGPVDRQVARTAQKEFKKQGLDIR
LGCRVTGTRVDGSVTVSYEDKNGKQELEVDRLIVCVGRRPNTDDLCSKEVDLLLDERGFINVDEDCKTNLPDVYAVGDVV
RGPMLAHKGQEEGIVVAERIAGKGGHLNYETIPWVIYTDPEIAWVGRTEEQLKKAGIPYNTGVFGFAANGRARAMDQTAG
MVKLIAHAETDRLLGAHIIGPQASELIAEAVVTMEFAGSAEDIARTIHAHPTLSETVHEAALAVGKRAIHKAN
>Mature_472_residues
SNHYDVIVIGAGPAGYSAAIRCAQLGMRTAVVDAFVFKDGEPALGGTCLNVGCIPSKALLDSSEQYHKVTHQLSAHGITA
QGVELDVEKMIARKDRVVKDLTGGIRQLFKANKIEWLHGHGQLVEAKTVEVRRPDGSTERHSADSVVLATGSQSVELGAA
PLDHERIVNSDRALDFTEVPERLGIIGAGVIGLEMGSVWSRLGSEVVLLEAQDEFLGPVDRQVARTAQKEFKKQGLDIRL
GCRVTGTRVDGSVTVSYEDKNGKQELEVDRLIVCVGRRPNTDDLCSKEVDLLLDERGFINVDEDCKTNLPDVYAVGDVVR
GPMLAHKGQEEGIVVAERIAGKGGHLNYETIPWVIYTDPEIAWVGRTEEQLKKAGIPYNTGVFGFAANGRARAMDQTAGM
VKLIAHAETDRLLGAHIIGPQASELIAEAVVTMEFAGSAEDIARTIHAHPTLSETVHEAALAVGKRAIHKAN

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=46.1538461538462, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI50301238, Length=474, Percent_Identity=28.2700421940928, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI22035672, Length=486, Percent_Identity=28.3950617283951, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI33519430, Length=464, Percent_Identity=23.7068965517241, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI33519428, Length=464, Percent_Identity=23.7068965517241, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI33519426, Length=464, Percent_Identity=23.7068965517241, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI148277065, Length=464, Percent_Identity=23.7068965517241, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI148277071, Length=464, Percent_Identity=23.7068965517241, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI291045266, Length=473, Percent_Identity=24.7357293868922, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI291045268, Length=468, Percent_Identity=24.1452991452991, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786307, Length=457, Percent_Identity=41.7943107221007, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=32.4210526315789, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=29.6137339055794, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1789915, Length=461, Percent_Identity=26.8980477223427, Blast_Score=158, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=48.068669527897, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17557007, Length=480, Percent_Identity=26.875, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=485, Percent_Identity=24.9484536082474, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=426, Percent_Identity=25.5868544600939, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=426, Percent_Identity=25.5868544600939, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=46.8879668049792, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6325240, Length=473, Percent_Identity=31.0782241014799, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=28.0254777070064, Blast_Score=170, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=49.244060475162, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=27.6288659793814, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=27.6288659793814, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=27.6288659793814, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17737741, Length=497, Percent_Identity=25.7545271629779, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51008; Mature: 50876

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNHYDVIVIGAGPAGYSAAIRCAQLGMRTAVVDAFVFKDGEPALGGTCLNVGCIPSKAL
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEECCCCCHHH
LDSSEQYHKVTHQLSAHGITAQGVELDVEKMIARKDRVVKDLTGGIRQLFKANKIEWLHG
HCCCHHHHHHHHHHHHCCCEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
HGQLVEAKTVEVRRPDGSTERHSADSVVLATGSQSVELGAAPLDHERIVNSDRALDFTEV
CCCEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCEEECCCCCCHHHHCCCCCCCCHHHH
PERLGIIGAGVIGLEMGSVWSRLGSEVVLLEAQDEFLGPVDRQVARTAQKEFKKQGLDIR
HHHHCEEECCHHHHHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHCCCCEE
LGCRVTGTRVDGSVTVSYEDKNGKQELEVDRLIVCVGRRPNTDDLCSKEVDLLLDERGFI
EEEEEEEEEECCEEEEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHEECCCCCC
NVDEDCKTNLPDVYAVGDVVRGPMLAHKGQEEGIVVAERIAGKGGHLNYETIPWVIYTDP
CCCCHHCCCCCCCEEHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEECC
EIAWVGRTEEQLKKAGIPYNTGVFGFAANGRARAMDQTAGMVKLIAHAETDRLLGAHIIG
CEEEECCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHEEEECC
PQASELIAEAVVTMEFAGSAEDIARTIHAHPTLSETVHEAALAVGKRAIHKAN
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNHYDVIVIGAGPAGYSAAIRCAQLGMRTAVVDAFVFKDGEPALGGTCLNVGCIPSKAL
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEECCCCCHHH
LDSSEQYHKVTHQLSAHGITAQGVELDVEKMIARKDRVVKDLTGGIRQLFKANKIEWLHG
HCCCHHHHHHHHHHHHCCCEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
HGQLVEAKTVEVRRPDGSTERHSADSVVLATGSQSVELGAAPLDHERIVNSDRALDFTEV
CCCEEEEEEEEEECCCCCCCCCCCCEEEEEECCCCEEECCCCCCHHHHCCCCCCCCHHHH
PERLGIIGAGVIGLEMGSVWSRLGSEVVLLEAQDEFLGPVDRQVARTAQKEFKKQGLDIR
HHHHCEEECCHHHHHHHHHHHHCCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHCCCCEE
LGCRVTGTRVDGSVTVSYEDKNGKQELEVDRLIVCVGRRPNTDDLCSKEVDLLLDERGFI
EEEEEEEEEECCEEEEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHEECCCCCC
NVDEDCKTNLPDVYAVGDVVRGPMLAHKGQEEGIVVAERIAGKGGHLNYETIPWVIYTDP
CCCCHHCCCCCCCEEHHHHHCCCHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEECC
EIAWVGRTEEQLKKAGIPYNTGVFGFAANGRARAMDQTAGMVKLIAHAETDRLLGAHIIG
CEEEECCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHEEEECC
PQASELIAEAVVTMEFAGSAEDIARTIHAHPTLSETVHEAALAVGKRAIHKAN
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]