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The map label for this gene is ybfF [H]

Identifier: 121997821

GI number: 121997821

Start: 1106436

End: 1107230

Strand: Reverse

Name: ybfF [H]

Synonym: Hhal_1030

Alternate gene names: 121997821

Gene position: 1107230-1106436 (Counterclockwise)

Preceding gene: 121997822

Following gene: 121997820

Centisome position: 41.34

GC content: 69.94

Gene sequence:

>795_bases
ATGAGCGAACCGGTGCAACTCTCCCTGACCACCCGCGGCACGGGGGCGCCGCTGTTGCTGCTCCACGGGCTCTACGGCAG
CAGTGGCAACCTCAACCGCTTCACGCGGCGCTTCGCCCACGACTATCGGGTGCTGGCCCCAGACCTGCGCAACCACGGCC
GCTCGCCCCATCGGCCCGGCATGGATTACCCGACGCTGACCGAGGATGTCGTGGCGCTGCTCGACCGGGAAGGCATCGAG
CGGGTGGCCGTGCTCGGTCACTCCATGGGCGGCAAGGTGGCCATGACCCTCGCACTGACCCGACCGCAGCGGGTAGCGGC
CGTGATCGCTGCGGACATCGCCCCGGTGACCTACCAGCATAACCACGGCACGCTGATCGGGGCGCTGCAGCAGGTCGACG
TGGCCGGCGCAGGCAATCGGCGCGAAGTGGATGCAGCGCTGGCGGCAGCGATCGACTCGCCGGCGGTGCGCCAGTTCCTC
CTCACCAACCTGGTCCCCGCTGACGAGGGCGGCTACCGTTGGCGTATCCCTCTCCAGTTCCTCGCCGACGCGGTCCCGGA
TATTCAGGGCTTCCCGGAGCTGGAGGGCGCCTACGACGGTCCGGCGCTGTTCCTCTACGGGACCGCCTCCGACTACTTCG
ATCCCGATCGCCACCGGGATGCCGTGGCGGCGTACTTCCCGAAGGCCGAATACGCGGCTCTCGAGGGGGCGGGCCACTGG
CTCCACGCCGAGCAGCCCGAGGCGTTCGCAGCTGCCGTGGAGCGGTTCCTGGCGCACGCCTATCCGGCGCGGTGA

Upstream 100 bases:

>100_bases
GGGGAGCGCCGTGCTGGCGGTGGCGGTCCAGGAACTCAAGGCGCTGGTCCAGGTGGGCGATGCCCGGCGTTTCTCCGAAC
CCTGAACGACACGAGGTCCT

Downstream 100 bases:

>100_bases
CGCCACCACCCTGGCAGCAAGCCTGAAGCTCACCCTATACTGGGCTGATCCAATACCCAAGGGATTTTATATGTCCGACG
ACTTCAAAGAAGCCGCGCTC

Product: alpha/beta hydrolase fold

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSEPVQLSLTTRGTGAPLLLLHGLYGSSGNLNRFTRRFAHDYRVLAPDLRNHGRSPHRPGMDYPTLTEDVVALLDREGIE
RVAVLGHSMGGKVAMTLALTRPQRVAAVIAADIAPVTYQHNHGTLIGALQQVDVAGAGNRREVDAALAAAIDSPAVRQFL
LTNLVPADEGGYRWRIPLQFLADAVPDIQGFPELEGAYDGPALFLYGTASDYFDPDRHRDAVAAYFPKAEYAALEGAGHW
LHAEQPEAFAAAVERFLAHAYPAR

Sequences:

>Translated_264_residues
MSEPVQLSLTTRGTGAPLLLLHGLYGSSGNLNRFTRRFAHDYRVLAPDLRNHGRSPHRPGMDYPTLTEDVVALLDREGIE
RVAVLGHSMGGKVAMTLALTRPQRVAAVIAADIAPVTYQHNHGTLIGALQQVDVAGAGNRREVDAALAAAIDSPAVRQFL
LTNLVPADEGGYRWRIPLQFLADAVPDIQGFPELEGAYDGPALFLYGTASDYFDPDRHRDAVAAYFPKAEYAALEGAGHW
LHAEQPEAFAAAVERFLAHAYPAR
>Mature_263_residues
SEPVQLSLTTRGTGAPLLLLHGLYGSSGNLNRFTRRFAHDYRVLAPDLRNHGRSPHRPGMDYPTLTEDVVALLDREGIER
VAVLGHSMGGKVAMTLALTRPQRVAAVIAADIAPVTYQHNHGTLIGALQQVDVAGAGNRREVDAALAAAIDSPAVRQFLL
TNLVPADEGGYRWRIPLQFLADAVPDIQGFPELEGAYDGPALFLYGTASDYFDPDRHRDAVAAYFPKAEYAALEGAGHWL
HAEQPEAFAAAVERFLAHAYPAR

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=249, Percent_Identity=40.5622489959839, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI23200008, Length=249, Percent_Identity=40.5622489959839, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI223941839, Length=243, Percent_Identity=35.3909465020576, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI1786902, Length=248, Percent_Identity=37.0967741935484, Blast_Score=171, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17508535, Length=257, Percent_Identity=36.9649805447471, Blast_Score=174, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17507063, Length=255, Percent_Identity=27.843137254902, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321468, Length=268, Percent_Identity=34.7014925373134, Blast_Score=116, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640348, Length=273, Percent_Identity=28.9377289377289, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24646055, Length=227, Percent_Identity=27.7533039647577, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28627; Mature: 28495

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPVQLSLTTRGTGAPLLLLHGLYGSSGNLNRFTRRFAHDYRVLAPDLRNHGRSPHRPG
CCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCCCCC
MDYPTLTEDVVALLDREGIERVAVLGHSMGGKVAMTLALTRPQRVAAVIAADIAPVTYQH
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCCEEEEC
NHGTLIGALQQVDVAGAGNRREVDAALAAAIDSPAVRQFLLTNLVPADEGGYRWRIPLQF
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEHHHH
LADAVPDIQGFPELEGAYDGPALFLYGTASDYFDPDRHRDAVAAYFPKAEYAALEGAGHW
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCHHHHHCCCHHHHHHCCCCCC
LHAEQPEAFAAAVERFLAHAYPAR
CCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEPVQLSLTTRGTGAPLLLLHGLYGSSGNLNRFTRRFAHDYRVLAPDLRNHGRSPHRPG
CCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEECCHHHHCCCCCCCCC
MDYPTLTEDVVALLDREGIERVAVLGHSMGGKVAMTLALTRPQRVAAVIAADIAPVTYQH
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHCCCEEEEC
NHGTLIGALQQVDVAGAGNRREVDAALAAAIDSPAVRQFLLTNLVPADEGGYRWRIPLQF
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEHHHH
LADAVPDIQGFPELEGAYDGPALFLYGTASDYFDPDRHRDAVAAYFPKAEYAALEGAGHW
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHCCHHHHHCCCHHHHHHCCCCCC
LHAEQPEAFAAAVERFLAHAYPAR
CCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]