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The map label for this gene is gatA

Identifier: 121997794

GI number: 121997794

Start: 1074938

End: 1076392

Strand: Reverse

Name: gatA

Synonym: Hhal_1003

Alternate gene names: 121997794

Gene position: 1076392-1074938 (Counterclockwise)

Preceding gene: 121997795

Following gene: 121997793

Centisome position: 40.19

GC content: 69.97

Gene sequence:

>1455_bases
ATGCATCAGAAGACCGTCGCCGAGATCTCCGCAGCACTGCACGCCGGAGAGATCTCTAGTCGTGAACTCACCGATTCCCT
GCTCAAGCGCATCGAGCAGTTCGATCCCAGGCTCAACAGCCTGATCACCGTCACCGCCGAGCAGGCGCGGGCCGCCGCCG
ACGCCGCCGACGCCCGGCTGCGCGCCGGCGAGGCCGGCCCGCTGACGGGCGTGCCGCTGGTCCACAAGGACATCTTCTGC
ACCGACGGCGTGGCGACCACCTGCGGCTCGCGCATGCTTGAGCCGTTTCGTGCCCCCTACGACGCGACCGTGGTGCGGCG
TCTGGCCGAGGCCGGTGCGGTGATGCTCGGCAAGGCCAACATGGACGAGTTCGCCATGGGGTCGTCCAACGAGACCAGCT
ACTTTGGTCCGGTGCGCAACCCCTGGGACCTGGACGCGGTGCCCGGCGGCTCCTCCGGCGGGTCGGCCGCTGCGGTGGCG
GCGCGCCTGGCGCCAGCGGCTACGGGCACCGACACCGGCGGCTCCATCCGGCAGCCGGCGGCCCTCTCCGGCATCTGCGG
GCTCAAGCCCACCTACGGCCGCGTATCGCGCTACGGCATGGTGGCCTTCGCCTCGAGCCTCGATCAGGCGGGTCCGTTCG
CCCGTACCGCCGAGGATCTGGCGCTGCTGCTCGGCGGCATGGCCGGCCTGGACGAGCGCGACTCGACCAGTGTCGATCAC
GTGGTGCCCGACTACAGCGAGTCGCTCAACCGCTCGATCAGAGGTCTGCGCATCGGTGTGCCGAAGGAGTTCTTCGGCGA
GGGGCTGGATGCTGACGTCCGCTCCCGGGTCGAGGCAGCGCTGCAGGTCCTGGAGGGCGAGGGCGCCGAGCTGGTCGAGG
TGCAGCTGCCCAACATGGAGCTGGCGCTGCCGGCGTACTACGTCATCGCACCGGCCGAGGCCTCGTCCAATCTGGCGCGC
TTCGACGGCGTGCGCTTCGGCCACCGCTGTGCCGATCCCCAGGACCTGGAAGACCTCTACAAGCGCAGCCGCGCCGAGGG
GTTCGGCGACGAGGTCCAGCGCCGCATCCTGGTGGGTACCTACGCGCTCTCCGCCGGCTACTACGACGCCTACTACCGCA
AGGCGCAGCAGGTTCGCCGGCTGGTGGCGGACGACTTCCGCCAGGCCCTGGATCAGGTCGACGTGCTGGCCGGCCCCACC
TCACCGACCCCGGCCTTCGACCTGGGCGAGCGCGCCGACGACCCGGTGCAGATGTACCTCTCCGACATCTACACCCTCGG
GGTGAACCTCGCCGGTCTGCCCGGCCTGTCGGTGCCCGCCGGTTTCTCCCGCGGGCGGCCCGTGGGGTTGCAGCTGATCG
GTGGTTACTTCGATGAGGCCCGGCTGCTCAACGTCGGGCACAAGTACCAGCAGGTCACGGACTGGCACAAGCAGGTTCCC
GAAGGGTTCGAGTAG

Upstream 100 bases:

>100_bases
GAACCCAATCTGCGCGAGCACTACCAGTCCGGGGCCCCGGCCGTCGAAGCCGGCCTGTACCTCGTCCCCCGCGTCGTCGA
GTAACGGAAGCCGAACGCCC

Downstream 100 bases:

>100_bases
ATGCGGGGCGACCCCGGACACCCTGGAACCAACGCCCGCGTCCGCCCGACGCGCCCAAAGCCTACGGAGCGACCTCACCC
ATGAAGTGGGAAACCGTCAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MHQKTVAEISAALHAGEISSRELTDSLLKRIEQFDPRLNSLITVTAEQARAAADAADARLRAGEAGPLTGVPLVHKDIFC
TDGVATTCGSRMLEPFRAPYDATVVRRLAEAGAVMLGKANMDEFAMGSSNETSYFGPVRNPWDLDAVPGGSSGGSAAAVA
ARLAPAATGTDTGGSIRQPAALSGICGLKPTYGRVSRYGMVAFASSLDQAGPFARTAEDLALLLGGMAGLDERDSTSVDH
VVPDYSESLNRSIRGLRIGVPKEFFGEGLDADVRSRVEAALQVLEGEGAELVEVQLPNMELALPAYYVIAPAEASSNLAR
FDGVRFGHRCADPQDLEDLYKRSRAEGFGDEVQRRILVGTYALSAGYYDAYYRKAQQVRRLVADDFRQALDQVDVLAGPT
SPTPAFDLGERADDPVQMYLSDIYTLGVNLAGLPGLSVPAGFSRGRPVGLQLIGGYFDEARLLNVGHKYQQVTDWHKQVP
EGFE

Sequences:

>Translated_484_residues
MHQKTVAEISAALHAGEISSRELTDSLLKRIEQFDPRLNSLITVTAEQARAAADAADARLRAGEAGPLTGVPLVHKDIFC
TDGVATTCGSRMLEPFRAPYDATVVRRLAEAGAVMLGKANMDEFAMGSSNETSYFGPVRNPWDLDAVPGGSSGGSAAAVA
ARLAPAATGTDTGGSIRQPAALSGICGLKPTYGRVSRYGMVAFASSLDQAGPFARTAEDLALLLGGMAGLDERDSTSVDH
VVPDYSESLNRSIRGLRIGVPKEFFGEGLDADVRSRVEAALQVLEGEGAELVEVQLPNMELALPAYYVIAPAEASSNLAR
FDGVRFGHRCADPQDLEDLYKRSRAEGFGDEVQRRILVGTYALSAGYYDAYYRKAQQVRRLVADDFRQALDQVDVLAGPT
SPTPAFDLGERADDPVQMYLSDIYTLGVNLAGLPGLSVPAGFSRGRPVGLQLIGGYFDEARLLNVGHKYQQVTDWHKQVP
EGFE
>Mature_484_residues
MHQKTVAEISAALHAGEISSRELTDSLLKRIEQFDPRLNSLITVTAEQARAAADAADARLRAGEAGPLTGVPLVHKDIFC
TDGVATTCGSRMLEPFRAPYDATVVRRLAEAGAVMLGKANMDEFAMGSSNETSYFGPVRNPWDLDAVPGGSSGGSAAAVA
ARLAPAATGTDTGGSIRQPAALSGICGLKPTYGRVSRYGMVAFASSLDQAGPFARTAEDLALLLGGMAGLDERDSTSVDH
VVPDYSESLNRSIRGLRIGVPKEFFGEGLDADVRSRVEAALQVLEGEGAELVEVQLPNMELALPAYYVIAPAEASSNLAR
FDGVRFGHRCADPQDLEDLYKRSRAEGFGDEVQRRILVGTYALSAGYYDAYYRKAQQVRRLVADDFRQALDQVDVLAGPT
SPTPAFDLGERADDPVQMYLSDIYTLGVNLAGLPGLSVPAGFSRGRPVGLQLIGGYFDEARLLNVGHKYQQVTDWHKQVP
EGFE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=39.3574297188755, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI195972892, Length=491, Percent_Identity=25.050916496945, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI166795287, Length=257, Percent_Identity=33.0739299610895, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=438, Percent_Identity=39.7260273972603, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17537465, Length=482, Percent_Identity=25.3112033195021, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=232, Percent_Identity=29.7413793103448, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=311, Percent_Identity=27.3311897106109, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=311, Percent_Identity=27.3311897106109, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=306, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=253, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=466, Percent_Identity=35.1931330472103, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=419, Percent_Identity=33.890214797136, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320448, Length=502, Percent_Identity=25.4980079681275, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648113, Length=482, Percent_Identity=40.8713692946058, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24652985, Length=503, Percent_Identity=27.6341948310139, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922090, Length=503, Percent_Identity=27.6341948310139, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652981, Length=503, Percent_Identity=27.6341948310139, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24652983, Length=503, Percent_Identity=27.6341948310139, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=26.078431372549, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI161078093, Length=520, Percent_Identity=27.8846153846154, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648435, Length=479, Percent_Identity=23.1732776617954, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648437, Length=479, Percent_Identity=23.1732776617954, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI45550774, Length=236, Percent_Identity=30.9322033898305, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21356731, Length=237, Percent_Identity=32.9113924050633, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648441, Length=412, Percent_Identity=24.0291262135922, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648439, Length=412, Percent_Identity=24.0291262135922, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_HALHL (A1WVR7)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002581.1
- ProteinModelPortal:   A1WVR7
- SMR:   A1WVR7
- STRING:   A1WVR7
- GeneID:   4709577
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1003
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   A1WVR7
- ProtClustDB:   PRK00012
- BioCyc:   HHAL349124:HHAL_1003-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51763; Mature: 51763

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQKTVAEISAALHAGEISSRELTDSLLKRIEQFDPRLNSLITVTAEQARAAADAADARL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEEEHHHHHHHHHHHHHHH
RAGEAGPLTGVPLVHKDIFCTDGVATTCGSRMLEPFRAPYDATVVRRLAEAGAVMLGKAN
HCCCCCCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCC
MDEFAMGSSNETSYFGPVRNPWDLDAVPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPA
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCH
ALSGICGLKPTYGRVSRYGMVAFASSLDQAGPFARTAEDLALLLGGMAGLDERDSTSVDH
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHH
VVPDYSESLNRSIRGLRIGVPKEFFGEGLDADVRSRVEAALQVLEGEGAELVEVQLPNME
CCCCHHHHHHHHHCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCE
LALPAYYVIAPAEASSNLARFDGVRFGHRCADPQDLEDLYKRSRAEGFGDEVQRRILVGT
EECCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YALSAGYYDAYYRKAQQVRRLVADDFRQALDQVDVLAGPTSPTPAFDLGERADDPVQMYL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
SDIYTLGVNLAGLPGLSVPAGFSRGRPVGLQLIGGYFDEARLLNVGHKYQQVTDWHKQVP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
EGFE
CCCC
>Mature Secondary Structure
MHQKTVAEISAALHAGEISSRELTDSLLKRIEQFDPRLNSLITVTAEQARAAADAADARL
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEEEHHHHHHHHHHHHHHH
RAGEAGPLTGVPLVHKDIFCTDGVATTCGSRMLEPFRAPYDATVVRRLAEAGAVMLGKAN
HCCCCCCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCC
MDEFAMGSSNETSYFGPVRNPWDLDAVPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPA
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCH
ALSGICGLKPTYGRVSRYGMVAFASSLDQAGPFARTAEDLALLLGGMAGLDERDSTSVDH
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHH
VVPDYSESLNRSIRGLRIGVPKEFFGEGLDADVRSRVEAALQVLEGEGAELVEVQLPNME
CCCCHHHHHHHHHCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCE
LALPAYYVIAPAEASSNLARFDGVRFGHRCADPQDLEDLYKRSRAEGFGDEVQRRILVGT
EECCEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YALSAGYYDAYYRKAQQVRRLVADDFRQALDQVDVLAGPTSPTPAFDLGERADDPVQMYL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
SDIYTLGVNLAGLPGLSVPAGFSRGRPVGLQLIGGYFDEARLLNVGHKYQQVTDWHKQVP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
EGFE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA