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The map label for this gene is gatB

Identifier: 121997793

GI number: 121997793

Start: 1073418

End: 1074857

Strand: Reverse

Name: gatB

Synonym: Hhal_1002

Alternate gene names: 121997793

Gene position: 1074857-1073418 (Counterclockwise)

Preceding gene: 121997794

Following gene: 121997792

Centisome position: 40.13

GC content: 66.67

Gene sequence:

>1440_bases
ATGAAGTGGGAAACCGTCATCGGTCTCGAGATCCACGCGCAGCTCGCGACCCGCACCAAGATCTTCTCCGGCGCGCCGAC
CGCCTACGGCGCCGAGCCGAACACCCAGGCCTGCCCGGTGGACCTCGGCCTACCCGGGGTCCTGCCCGTGCTCAACCGCG
AAGTGGTGCGCATGGCCATCAAGTTCGGCCTGGCCGTCGACGCGCGGATCGCGCCGCGCTCGGTCTTCGCGCGCAAGAAC
TACTTCTACCCCGACCTGCCCAAGGGGTATCAGATCTCGCAGTACGACCTGCCCATCGTCGAGGGCGGGCACCTGGACAT
TGAGCTCGAGGACGGCGCCAGCAAGCGGATCGGCATCACCCGCGCCCACCTCGAGGAGGATGCCGGCAAGTCCCTGCACG
AAGACTTCCACGGCATGACCGGGGTCGATCTCAACCGTGCCGGTACGCCGCTGATGGAGATCGTCTCGGAACCGGATCTG
CGCTCGCCGGCCGAGGCCGCCGCCTACATGAAGAAGCTCCACGCCCTGGTGCGCTATCTGGAGATCTGCGACGGCAACAT
GCAGGAGGGGTCCTTCCGCTGCGACGCCAACGTCTCGGTGCGCCCCGTCGGGCAGGAGGCGTTCGGCACCCGGGCCGAGC
TCAAGAACCTCAACTCCTTTCGCTTCGTCGAGCGGGCCCTCGAGTACGAGGTCGAGCGGCAGATCGACCTGCTCGAATCC
GGCGGCGAGGTGGTGCAGGAGACCCGCCTCTACGACGTCGACAAGGGCGTCACCCGCTCCATGCGGACCAAGGAAGAGGC
CAACGACTACCGTTACTTCCCGGAGCCGGACCTGCTCCCCGTGGAGGTGGATGCGGCGCTGGTCGACGAGGTGCGGGAGA
CCCTGCCCGAACTGCCCGACGAGAAACGCCGGCGGTTCGAGGAGGAGTACGGCCTGCCGGCCTACGACGCCGGTGTGCTG
ACGGCCACCCGGGACATGGCCGATTTCTTCGAGCACGCCGTCAGCGAGTCCGGCGGCTTCGCCAAGCGCACCGCCAACCT
GCTGATGAGCGAGCTGCTGGCCTACCTGAACAAGGACGGCCTGGAGATCGCCGAATCACCGGTCACCCCGGAGATGCTGG
GCAAGCTGGTGGCCCGGGTCGAGGACGAAACCCTCTCGAGCCGCGGTGCCAAGGATGTCTTCGAGGCCATGTGGGCGGGC
GAAGGCGAGCCGGACGAAGTCATCGAGAAGAAGGGACTCAAGCAGGTCACCGACACCTCGGCCATCGAGGCGCTGGTCGA
CGAGGCCATCGCCAACAACCCGCAGCAGCTCGAGCAGTACCGCGCCGGCAAGGAGAAGCTCTTCGGCTTCTTTGTCGGTC
AGGTGATGAAGGCCTCCGGCGGCAAGGCGAACCCGCAGCAGGTCAACGAGCTGCTCAAGAAGAAGCTGGACGGATCGTGA

Upstream 100 bases:

>100_bases
TTCCCGAAGGGTTCGAGTAGATGCGGGGCGACCCCGGACACCCTGGAACCAACGCCCGCGTCCGCCCGACGCGCCCAAAG
CCTACGGAGCGACCTCACCC

Downstream 100 bases:

>100_bases
GCGACGCCAGTCAACGGTTTCTCTTTGAAAACGCCGACGTACGCGGCGAGATTGTACAGCTGGAGCAGGCGTTCGCCGCC
GTCCGCGAGCACCACGACTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKWETVIGLEIHAQLATRTKIFSGAPTAYGAEPNTQACPVDLGLPGVLPVLNREVVRMAIKFGLAVDARIAPRSVFARKN
YFYPDLPKGYQISQYDLPIVEGGHLDIELEDGASKRIGITRAHLEEDAGKSLHEDFHGMTGVDLNRAGTPLMEIVSEPDL
RSPAEAAAYMKKLHALVRYLEICDGNMQEGSFRCDANVSVRPVGQEAFGTRAELKNLNSFRFVERALEYEVERQIDLLES
GGEVVQETRLYDVDKGVTRSMRTKEEANDYRYFPEPDLLPVEVDAALVDEVRETLPELPDEKRRRFEEEYGLPAYDAGVL
TATRDMADFFEHAVSESGGFAKRTANLLMSELLAYLNKDGLEIAESPVTPEMLGKLVARVEDETLSSRGAKDVFEAMWAG
EGEPDEVIEKKGLKQVTDTSAIEALVDEAIANNPQQLEQYRAGKEKLFGFFVGQVMKASGGKANPQQVNELLKKKLDGS

Sequences:

>Translated_479_residues
MKWETVIGLEIHAQLATRTKIFSGAPTAYGAEPNTQACPVDLGLPGVLPVLNREVVRMAIKFGLAVDARIAPRSVFARKN
YFYPDLPKGYQISQYDLPIVEGGHLDIELEDGASKRIGITRAHLEEDAGKSLHEDFHGMTGVDLNRAGTPLMEIVSEPDL
RSPAEAAAYMKKLHALVRYLEICDGNMQEGSFRCDANVSVRPVGQEAFGTRAELKNLNSFRFVERALEYEVERQIDLLES
GGEVVQETRLYDVDKGVTRSMRTKEEANDYRYFPEPDLLPVEVDAALVDEVRETLPELPDEKRRRFEEEYGLPAYDAGVL
TATRDMADFFEHAVSESGGFAKRTANLLMSELLAYLNKDGLEIAESPVTPEMLGKLVARVEDETLSSRGAKDVFEAMWAG
EGEPDEVIEKKGLKQVTDTSAIEALVDEAIANNPQQLEQYRAGKEKLFGFFVGQVMKASGGKANPQQVNELLKKKLDGS
>Mature_479_residues
MKWETVIGLEIHAQLATRTKIFSGAPTAYGAEPNTQACPVDLGLPGVLPVLNREVVRMAIKFGLAVDARIAPRSVFARKN
YFYPDLPKGYQISQYDLPIVEGGHLDIELEDGASKRIGITRAHLEEDAGKSLHEDFHGMTGVDLNRAGTPLMEIVSEPDL
RSPAEAAAYMKKLHALVRYLEICDGNMQEGSFRCDANVSVRPVGQEAFGTRAELKNLNSFRFVERALEYEVERQIDLLES
GGEVVQETRLYDVDKGVTRSMRTKEEANDYRYFPEPDLLPVEVDAALVDEVRETLPELPDEKRRRFEEEYGLPAYDAGVL
TATRDMADFFEHAVSESGGFAKRTANLLMSELLAYLNKDGLEIAESPVTPEMLGKLVARVEDETLSSRGAKDVFEAMWAG
EGEPDEVIEKKGLKQVTDTSAIEALVDEAIANNPQQLEQYRAGKEKLFGFFVGQVMKASGGKANPQQVNELLKKKLDGS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=496, Percent_Identity=39.1129032258064, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI115534094, Length=480, Percent_Identity=31.6666666666667, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=515, Percent_Identity=30.873786407767, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=37.0672097759674, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_HALHL (A1WVR6)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002580.1
- ProteinModelPortal:   A1WVR6
- SMR:   A1WVR6
- STRING:   A1WVR6
- GeneID:   4709576
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1002
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   A1WVR6
- ProtClustDB:   PRK05477
- BioCyc:   HHAL349124:HHAL_1002-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53121; Mature: 53121

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWETVIGLEIHAQLATRTKIFSGAPTAYGAEPNTQACPVDLGLPGVLPVLNREVVRMAI
CCCCEEEEHEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KFGLAVDARIAPRSVFARKNYFYPDLPKGYQISQYDLPIVEGGHLDIELEDGASKRIGIT
HHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCHH
RAHLEEDAGKSLHEDFHGMTGVDLNRAGTPLMEIVSEPDLRSPAEAAAYMKKLHALVRYL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
EICDGNMQEGSFRCDANVSVRPVGQEAFGTRAELKNLNSFRFVERALEYEVERQIDLLES
HHHCCCCCCCCEEECCCCEEEECCHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GGEVVQETRLYDVDKGVTRSMRTKEEANDYRYFPEPDLLPVEVDAALVDEVRETLPELPD
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCC
EKRRRFEEEYGLPAYDAGVLTATRDMADFFEHAVSESGGFAKRTANLLMSELLAYLNKDG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
LEIAESPVTPEMLGKLVARVEDETLSSRGAKDVFEAMWAGEGEPDEVIEKKGLKQVTDTS
CHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHH
AIEALVDEAIANNPQQLEQYRAGKEKLFGFFVGQVMKASGGKANPQQVNELLKKKLDGS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKWETVIGLEIHAQLATRTKIFSGAPTAYGAEPNTQACPVDLGLPGVLPVLNREVVRMAI
CCCCEEEEHEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KFGLAVDARIAPRSVFARKNYFYPDLPKGYQISQYDLPIVEGGHLDIELEDGASKRIGIT
HHCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCCHH
RAHLEEDAGKSLHEDFHGMTGVDLNRAGTPLMEIVSEPDLRSPAEAAAYMKKLHALVRYL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
EICDGNMQEGSFRCDANVSVRPVGQEAFGTRAELKNLNSFRFVERALEYEVERQIDLLES
HHHCCCCCCCCEEECCCCEEEECCHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GGEVVQETRLYDVDKGVTRSMRTKEEANDYRYFPEPDLLPVEVDAALVDEVRETLPELPD
CHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCC
EKRRRFEEEYGLPAYDAGVLTATRDMADFFEHAVSESGGFAKRTANLLMSELLAYLNKDG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
LEIAESPVTPEMLGKLVARVEDETLSSRGAKDVFEAMWAGEGEPDEVIEKKGLKQVTDTS
CHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHH
AIEALVDEAIANNPQQLEQYRAGKEKLFGFFVGQVMKASGGKANPQQVNELLKKKLDGS
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA