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The map label for this gene is amiC [H]

Identifier: 121997466

GI number: 121997466

Start: 752040

End: 753332

Strand: Reverse

Name: amiC [H]

Synonym: Hhal_0669

Alternate gene names: 121997466

Gene position: 753332-752040 (Counterclockwise)

Preceding gene: 121997467

Following gene: 121997465

Centisome position: 28.13

GC content: 67.52

Gene sequence:

>1293_bases
TTGAGGTGGGGCTATGGCCTGGCTTGGATCGGGGTGCTGATCGGCCTGTTGGTGGCCGGCGTGGCGTCGGCCGCGTCCCT
CGATGATGTGCGCCTTTCCGACGCGGACGACCGCACACGGGTGGTCTTCGACCTGGACCGGGTGCCCGAGCACCGGGTCT
TCAGCCTGCCGGATCCGCACCGGGTGGTCATCGATTTCGAGGGGGTGGAGCTCAACGCTGCAGACCTCCCCCGAGGCGGC
GTTCTCCAGGAGATCCGCACTGGCCGGCAGGAAGACGGCGGGCTGCGGGTCGTCCTCGATGTCAGCCGCGAGGTGGAGGC
GCGCAGCTTCGTCATCGGCGGCGAGGACGGCGGTCAGCGTCTGGTGGTGGATCTCGGCGGCCAGCAGGGGGGGCGCCGTC
AGGCGGTGCGCTCGGCCTCTGAGCGCGAGGCGCGGGATGTGATCGTGGCCATCGACGCCGGCCACGGCGGAGTCGATCCC
GGCGCCATCGGTCCCGAGGGGACCTTCGAGAAAGACGTGGCGTTGAGTGTTGCGCGCAAGCTCTACGACCTGATGACCGA
GGCGCCTGGGCTGAAGCCGCTGTTGGTGCGCGAGGGTGACTACTACATGAACCTGCGCGATCGCACCCGGGTGGCCCGCG
AAGGCAATGCCGATATCTTCCTGTCCATCCATGCCGATGGCGCCGAAAATCCCAATGTTAAAGGCGCATCGGTCTACGCC
CTGTCGGTGGACGGTGCCACCTCGGAGCAGGCGCGGGTGCTGGCGCGCCGGGAGAACGCGGCCGACTTTATCGGCGGGGT
GTCGCTGGAGGACAAGGACGACACGGTGGCCTCGGTATTGGTGGACCTCTCCCGGGGGCATACCATCGAGGCAAGCCTGG
AGATGGGCGAACACCTCCTGCCCAAGCTCGATCGCCACGCCGATCTGCTGCGCAACCGCGTCGATCAGGCCGGCTTTGCC
GTGCTCAAGTCCCTGGACATGCCCTCGCTGTTGATCGAGCTGGGCTTTCTCACCAACCCCGAGGAGGAGCGCCGGCTCAA
TACCCTCAGCTACCAGCGCGACCTCGCCGAGGGCATCGTCGCTGGTGTGCGCGAGTACGCCGAGCGCAACATCCTCCCCG
AGCTGCGGATGGCCGACGCCGGCCAGAACGGGCAGCGCGAGCACGAGGTTCAGCGGGGCGAGAATCTCTCGGTGATCGCC
CAGCAGTACCAGGTCAGCACCGAGCGTCTGCGCGAGGTCAATGACCTCTCCGGGGATGGCATCCGTGCTGGCAGCACCCT
GATCATCCCTTAG

Upstream 100 bases:

>100_bases
TTATCTCCCCGTTGAGTGGGCAAAAACTCTTGCAGCGGGTCGTGCGCCCGACATAGCCTTGAGGGTGCGTAGAAGCCACC
GCACGGTGGGAGGAGGATCT

Downstream 100 bases:

>100_bases
AATGCGCGCATGACGACGCGCGGTATTCGTCCCCTGCCGGATAACCTGATCGACCAGATCGCCGCTGGCGAGGTGGTCGA
GCGCCCCGGTTCGGTGGTCA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MRWGYGLAWIGVLIGLLVAGVASAASLDDVRLSDADDRTRVVFDLDRVPEHRVFSLPDPHRVVIDFEGVELNAADLPRGG
VLQEIRTGRQEDGGLRVVLDVSREVEARSFVIGGEDGGQRLVVDLGGQQGGRRQAVRSASEREARDVIVAIDAGHGGVDP
GAIGPEGTFEKDVALSVARKLYDLMTEAPGLKPLLVREGDYYMNLRDRTRVAREGNADIFLSIHADGAENPNVKGASVYA
LSVDGATSEQARVLARRENAADFIGGVSLEDKDDTVASVLVDLSRGHTIEASLEMGEHLLPKLDRHADLLRNRVDQAGFA
VLKSLDMPSLLIELGFLTNPEEERRLNTLSYQRDLAEGIVAGVREYAERNILPELRMADAGQNGQREHEVQRGENLSVIA
QQYQVSTERLREVNDLSGDGIRAGSTLIIP

Sequences:

>Translated_430_residues
MRWGYGLAWIGVLIGLLVAGVASAASLDDVRLSDADDRTRVVFDLDRVPEHRVFSLPDPHRVVIDFEGVELNAADLPRGG
VLQEIRTGRQEDGGLRVVLDVSREVEARSFVIGGEDGGQRLVVDLGGQQGGRRQAVRSASEREARDVIVAIDAGHGGVDP
GAIGPEGTFEKDVALSVARKLYDLMTEAPGLKPLLVREGDYYMNLRDRTRVAREGNADIFLSIHADGAENPNVKGASVYA
LSVDGATSEQARVLARRENAADFIGGVSLEDKDDTVASVLVDLSRGHTIEASLEMGEHLLPKLDRHADLLRNRVDQAGFA
VLKSLDMPSLLIELGFLTNPEEERRLNTLSYQRDLAEGIVAGVREYAERNILPELRMADAGQNGQREHEVQRGENLSVIA
QQYQVSTERLREVNDLSGDGIRAGSTLIIP
>Mature_430_residues
MRWGYGLAWIGVLIGLLVAGVASAASLDDVRLSDADDRTRVVFDLDRVPEHRVFSLPDPHRVVIDFEGVELNAADLPRGG
VLQEIRTGRQEDGGLRVVLDVSREVEARSFVIGGEDGGQRLVVDLGGQQGGRRQAVRSASEREARDVIVAIDAGHGGVDP
GAIGPEGTFEKDVALSVARKLYDLMTEAPGLKPLLVREGDYYMNLRDRTRVAREGNADIFLSIHADGAENPNVKGASVYA
LSVDGATSEQARVLARRENAADFIGGVSLEDKDDTVASVLVDLSRGHTIEASLEMGEHLLPKLDRHADLLRNRVDQAGFA
VLKSLDMPSLLIELGFLTNPEEERRLNTLSYQRDLAEGIVAGVREYAERNILPELRMADAGQNGQREHEVQRGENLSVIA
QQYQVSTERLREVNDLSGDGIRAGSTLIIP

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=398, Percent_Identity=34.9246231155779, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1788776, Length=219, Percent_Identity=44.2922374429224, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1790611, Length=238, Percent_Identity=40.7563025210084, Blast_Score=181, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46803; Mature: 46803

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWGYGLAWIGVLIGLLVAGVASAASLDDVRLSDADDRTRVVFDLDRVPEHRVFSLPDPH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCCCCEECCCCCC
RVVIDFEGVELNAADLPRGGVLQEIRTGRQEDGGLRVVLDVSREVEARSFVIGGEDGGQR
EEEEEECCEEECCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCE
LVVDLGGQQGGRRQAVRSASEREARDVIVAIDAGHGGVDPGAIGPEGTFEKDVALSVARK
EEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
LYDLMTEAPGLKPLLVREGDYYMNLRDRTRVAREGNADIFLSIHADGAENPNVKGASVYA
HHHHHHCCCCCCEEEEECCCEEECCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEE
LSVDGATSEQARVLARRENAADFIGGVSLEDKDDTVASVLVDLSRGHTIEASLEMGEHLL
EEECCCCCHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHH
PKLDRHADLLRNRVDQAGFAVLKSLDMPSLLIELGFLTNPEEERRLNTLSYQRDLAEGIV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AGVREYAERNILPELRMADAGQNGQREHEVQRGENLSVIAQQYQVSTERLREVNDLSGDG
HHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
IRAGSTLIIP
CCCCCEEECC
>Mature Secondary Structure
MRWGYGLAWIGVLIGLLVAGVASAASLDDVRLSDADDRTRVVFDLDRVPEHRVFSLPDPH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEEEECCCCCCCEECCCCCC
RVVIDFEGVELNAADLPRGGVLQEIRTGRQEDGGLRVVLDVSREVEARSFVIGGEDGGQR
EEEEEECCEEECCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCE
LVVDLGGQQGGRRQAVRSASEREARDVIVAIDAGHGGVDPGAIGPEGTFEKDVALSVARK
EEEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
LYDLMTEAPGLKPLLVREGDYYMNLRDRTRVAREGNADIFLSIHADGAENPNVKGASVYA
HHHHHHCCCCCCEEEEECCCEEECCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEE
LSVDGATSEQARVLARRENAADFIGGVSLEDKDDTVASVLVDLSRGHTIEASLEMGEHLL
EEECCCCCHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHH
PKLDRHADLLRNRVDQAGFAVLKSLDMPSLLIELGFLTNPEEERRLNTLSYQRDLAEGIV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AGVREYAERNILPELRMADAGQNGQREHEVQRGENLSVIAQQYQVSTERLREVNDLSGDG
HHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
IRAGSTLIIP
CCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]