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The map label for this gene is folD

Identifier: 121997411

GI number: 121997411

Start: 688969

End: 689841

Strand: Reverse

Name: folD

Synonym: Hhal_0610

Alternate gene names: 121997411

Gene position: 689841-688969 (Counterclockwise)

Preceding gene: 121997414

Following gene: 121997410

Centisome position: 25.76

GC content: 67.35

Gene sequence:

>873_bases
ATGCCTGCTCAGATCTTGGATGGGAAGGCCATCGCTGCCAAGCGTCGCGCGCTGGTGGGCGAGGCTGTCGGTCAGCGTAT
CGAACGCGGACTGCGTCGTCCCGGCCTCGCTGTCATCCTCGTCGGCCAGAACCCGGCCTCGGCGGTGTACGTCCGGAACA
AGCGGCGCGCGTGTGAGGAGGCCGGTATCCTCTCCCGGGCCTACGATCTGGACGCCGATGTCAGCGAGGGCGAGCTGCTC
GAGCGCATCGATCGGCTCAATGCCGACCCGGAGATCGATGGCATCCTCGTGCAGCTGCCGCTGCCCGGTCATATCGACGA
GCAGACGGTCATCGAGCGCATCGATCCGGTCAAGGATGTCGATGGGTTTCACCCCGAGAATATGGGCCGTCTGGCGTTGC
GGTTGCCGGGGCTGCGCCCGTGCACGCCGCGGGGTGTCATGACCCTGCTGCACCACACCGGCATCGAGCTGGAGGGGCGG
GATGCGGTGGTTCTCGGTCAGTCGAACATCGTCGGTCGCCCCATGACCCTGGAGCTGCTCAATGCCCGGTGCACGGTCAC
GGTGTGCCACAGCCGCACCCGCAACGTGGCGGAGCGGGTCCGTCAGGCGGATCTGGTGGTGGCTTCGGTGGGCAAGCCGG
GGCTGATCCAGGGCGACTGGATTGGAGAGGGGGCGGTGGTGATCGATGTGGGCATCAATCGCCTGGACAGCGGCAAGCTC
ACCGGTGATGTCGATTTCGAAGCGGCCAGTCAGCGCGCCTCGTGGATCACGCCGGTGCCTGGCGGGGTCGGTCCGATGAC
GGTGGCGACGCTCCTCGAGAACACCCTCGACGCGGCCCGCAACCGCGACGAACTCACGGCGGCGGCGTGCTAA

Upstream 100 bases:

>100_bases
TCCCGCATCCCGGCGTCAATTCCCCCCGCCCGTGCCGCCGTTGCCGGCGCCCCTGCGGCGTGCGAAAATCCGCGTCTATT
CGCGGTTTCTCGGGGGATTC

Downstream 100 bases:

>100_bases
GCTCACCACGGATGGTTTGACAGGGACAGGGAGTCGGATGGGCACGCAAGAAAAGCGCCGATTCAGCCGGGTGACCTTCC
ACGCGCCGGCTCGCCTGGTG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MPAQILDGKAIAAKRRALVGEAVGQRIERGLRRPGLAVILVGQNPASAVYVRNKRRACEEAGILSRAYDLDADVSEGELL
ERIDRLNADPEIDGILVQLPLPGHIDEQTVIERIDPVKDVDGFHPENMGRLALRLPGLRPCTPRGVMTLLHHTGIELEGR
DAVVLGQSNIVGRPMTLELLNARCTVTVCHSRTRNVAERVRQADLVVASVGKPGLIQGDWIGEGAVVIDVGINRLDSGKL
TGDVDFEAASQRASWITPVPGGVGPMTVATLLENTLDAARNRDELTAAAC

Sequences:

>Translated_290_residues
MPAQILDGKAIAAKRRALVGEAVGQRIERGLRRPGLAVILVGQNPASAVYVRNKRRACEEAGILSRAYDLDADVSEGELL
ERIDRLNADPEIDGILVQLPLPGHIDEQTVIERIDPVKDVDGFHPENMGRLALRLPGLRPCTPRGVMTLLHHTGIELEGR
DAVVLGQSNIVGRPMTLELLNARCTVTVCHSRTRNVAERVRQADLVVASVGKPGLIQGDWIGEGAVVIDVGINRLDSGKL
TGDVDFEAASQRASWITPVPGGVGPMTVATLLENTLDAARNRDELTAAAC
>Mature_289_residues
PAQILDGKAIAAKRRALVGEAVGQRIERGLRRPGLAVILVGQNPASAVYVRNKRRACEEAGILSRAYDLDADVSEGELLE
RIDRLNADPEIDGILVQLPLPGHIDEQTVIERIDPVKDVDGFHPENMGRLALRLPGLRPCTPRGVMTLLHHTGIELEGRD
AVVLGQSNIVGRPMTLELLNARCTVTVCHSRTRNVAERVRQADLVVASVGKPGLIQGDWIGEGAVVIDVGINRLDSGKLT
GDVDFEAASQRASWITPVPGGVGPMTVATLLENTLDAARNRDELTAAAC

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=45.9183673469388, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=45.2380952380952, Blast_Score=218, Evalue=3e-57,
Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=43.298969072165, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI36796743, Length=210, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=64.7482014388489, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=40.9556313993174, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=47.5694444444444, Blast_Score=250, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=44.3708609271523, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6322933, Length=292, Percent_Identity=25.3424657534247, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=49.3243243243243, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=49.3243243243243, Blast_Score=252, Evalue=2e-67,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=44.9477351916376, Blast_Score=220, Evalue=7e-58,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=44.9477351916376, Blast_Score=220, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=44.9477351916376, Blast_Score=220, Evalue=8e-58,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=44.9477351916376, Blast_Score=220, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_HALHL (A1WUN4)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001002198.1
- ProteinModelPortal:   A1WUN4
- SMR:   A1WUN4
- STRING:   A1WUN4
- GeneID:   4711447
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_0610
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   HELPAST
- PhylomeDB:   A1WUN4
- ProtClustDB:   PRK10792
- BioCyc:   HHAL349124:HHAL_0610-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31133; Mature: 31002

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQILDGKAIAAKRRALVGEAVGQRIERGLRRPGLAVILVGQNPASAVYVRNKRRACEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCHHHHHHH
AGILSRAYDLDADVSEGELLERIDRLNADPEIDGILVQLPLPGHIDEQTVIERIDPVKDV
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCHHHC
DGFHPENMGRLALRLPGLRPCTPRGVMTLLHHTGIELEGRDAVVLGQSNIVGRPMTLELL
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEECCCCEEEECCCCCCCCCEEEEEE
NARCTVTVCHSRTRNVAERVRQADLVVASVGKPGLIQGDWIGEGAVVIDVGINRLDSGKL
CCEEEEEEECHHHHHHHHHHHHHHEEEEECCCCCCEECCCCCCCEEEEEECCCCCCCCCC
TGDVDFEAASQRASWITPVPGGVGPMTVATLLENTLDAARNRDELTAAAC
CCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure 
PAQILDGKAIAAKRRALVGEAVGQRIERGLRRPGLAVILVGQNPASAVYVRNKRRACEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCHHHHHHH
AGILSRAYDLDADVSEGELLERIDRLNADPEIDGILVQLPLPGHIDEQTVIERIDPVKDV
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCHHHC
DGFHPENMGRLALRLPGLRPCTPRGVMTLLHHTGIELEGRDAVVLGQSNIVGRPMTLELL
CCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEECCCCEEEECCCCCCCCCEEEEEE
NARCTVTVCHSRTRNVAERVRQADLVVASVGKPGLIQGDWIGEGAVVIDVGINRLDSGKL
CCEEEEEEECHHHHHHHHHHHHHHEEEEECCCCCCEECCCCCCCEEEEEECCCCCCCCCC
TGDVDFEAASQRASWITPVPGGVGPMTVATLLENTLDAARNRDELTAAAC
CCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA