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The map label for this gene is nuoB [H]

Identifier: 121639029

GI number: 121639029

Start: 3465124

End: 3465678

Strand: Direct

Name: nuoB [H]

Synonym: BCG_3169

Alternate gene names: 121639029

Gene position: 3465124-3465678 (Clockwise)

Preceding gene: 121639028

Following gene: 121639030

Centisome position: 79.21

GC content: 62.16

Gene sequence:

>555_bases
GTGGGACTGGAAGAACAGCTGCCCGGCGGGATCCTGCTGTCGACCGTCGAGAAGGTGGCGGGCTATGTCCGCAAAAACTC
CCTGTGGCCGGCAACATTCGGATTGGCGTGCTGTGCGATCGAGATGATGGCGACCGCGGGACCAAGGTTTGACATTGCGC
GGTTCGGGATGGAACGGTTCTCGGCCACGCCGCGGCAGGCAGATCTGATGATCGTGGCGGGCCGGGTCAGCCAGAAGATG
GCGCCGGTACTGCGCCAGATCTATGACCAGATGGCGGAGCCGAAATGGGTTCTGGCCATGGGTGTGTGCGCCTCGTCAGG
TGGGATGTTCAACAACTATGCGATCGTGCAGGGCGTGGATCATGTTGTTCCGGTCGACATCTACCTACCCGGCTGCCCGC
CGCGCCCGGAGATGCTGCTGCACGCAATCCTGAAGCTGCACGAAAAGATTCAGCAGATGCCATTAGGTATCAACCGGGAA
CGCGCTATCGCCGAGGCCGAAGAGGCGGCGTTGTTGGCCCGGCCCACCATCGAGATGCGCGGACTGCTGCGATGA

Upstream 100 bases:

>100_bases
CGTTCGCGCTGGTCGAGATGGCGATATTCATGCTCACGGTGTTCGTGGCCTACGCGTATGTGTGGCGCCGCGGGGGCCTG
ACGTGGGATTGAGGTAGGGC

Downstream 100 bases:

>100_bases
GCCCGCCGAACCAAGACGCCCAGGAAGGCCGCCCGGACTCCCCCACCGCGGAGGTGGTCGACGTTCGCCGCGGCATGTTC
GGCGTCTCGGGCACCGGTGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MGLEEQLPGGILLSTVEKVAGYVRKNSLWPATFGLACCAIEMMATAGPRFDIARFGMERFSATPRQADLMIVAGRVSQKM
APVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLLHAILKLHEKIQQMPLGINRE
RAIAEAEEAALLARPTIEMRGLLR

Sequences:

>Translated_184_residues
MGLEEQLPGGILLSTVEKVAGYVRKNSLWPATFGLACCAIEMMATAGPRFDIARFGMERFSATPRQADLMIVAGRVSQKM
APVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLLHAILKLHEKIQQMPLGINRE
RAIAEAEEAALLARPTIEMRGLLR
>Mature_183_residues
GLEEQLPGGILLSTVEKVAGYVRKNSLWPATFGLACCAIEMMATAGPRFDIARFGMERFSATPRQADLMIVAGRVSQKMA
PVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLLHAILKLHEKIQQMPLGINRER
AIAEAEEAALLARPTIEMRGLLR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=145, Percent_Identity=53.1034482758621, Blast_Score=177, Evalue=6e-45,
Organism=Escherichia coli, GI1788624, Length=148, Percent_Identity=51.3513513513513, Blast_Score=182, Evalue=8e-48,
Organism=Escherichia coli, GI1789074, Length=112, Percent_Identity=40.1785714285714, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788834, Length=135, Percent_Identity=34.0740740740741, Blast_Score=85, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=141, Percent_Identity=53.9007092198582, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24651058, Length=154, Percent_Identity=50.6493506493506, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI18859983, Length=142, Percent_Identity=52.112676056338, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24642371, Length=142, Percent_Identity=52.112676056338, Blast_Score=176, Evalue=6e-45,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20219; Mature: 20088

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
8.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLEEQLPGGILLSTVEKVAGYVRKNSLWPATFGLACCAIEMMATAGPRFDIARFGMERF
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SATPRQADLMIVAGRVSQKMAPVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVD
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCHHHCCCC
HVVPVDIYLPGCPPRPEMLLHAILKLHEKIQQMPLGINRERAIAEAEEAALLARPTIEMR
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
GLLR
CCCC
>Mature Secondary Structure 
GLEEQLPGGILLSTVEKVAGYVRKNSLWPATFGLACCAIEMMATAGPRFDIARFGMERF
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SATPRQADLMIVAGRVSQKMAPVLRQIYDQMAEPKWVLAMGVCASSGGMFNNYAIVQGVD
CCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCHHHCCCC
HVVPVDIYLPGCPPRPEMLLHAILKLHEKIQQMPLGINRERAIAEAEEAALLARPTIEMR
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
GLLR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA