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The map label for this gene is cysA3

Identifier: 121639002

GI number: 121639002

Start: 3438460

End: 3439422

Strand: Direct

Name: cysA3

Synonym: BCG_3142

Alternate gene names: 121639002

Gene position: 3438460-3439422 (Clockwise)

Preceding gene: 121639001

Following gene: 121639003

Centisome position: 78.6

GC content: 60.12

Gene sequence:

>963_bases
ATGGCACGCTGCGATGTCCTGGTCTCCGCCGACTGGGCTGAGAGCAATCTGCACGCGCCGAAGGTCGTTTTCGTCGAAGT
GGACGAGGACACCAGTGCATATGACCGTGACCATATTGCCGGCGCGATCAAGTTGGACTGGCGCACCGACCTGCAGGATC
CGGTCAAACGTGACTTCGTCGACGCCCAGCAATTCTCCAAGCTGCTGTCCGAGCGTGGCATCGCCAACGAGGACACGGTG
ATCCTGTACGGCGGCAACAACAATTGGTTCGCCGCCTACGCGTACTGGTATTTCAAGCTCTACGGCCATGAGAAGGTCAA
GTTGCTCGACGGCGGCCGCAAGAAGTGGGAGCTCGACGGACGCCCGCTGTCCAGCGACCCGGTCAGCCGGCCGGTGACCT
CCTACACCGCCTCCCCGCCGGATAACACGATTCGGGCATTCCGCGACGAGGTCCTGGCGGCCATCAACGTCAAGAACCTC
ATCGACGTGCGCTCTCCCGACGAGTTCTCCGGCAAGATCCTGGCCCCCGCGCACCTGCCGCAGGAACAAAGCCAGCGGCC
CGGACACATTCCTGGTGCCATCAACGTGCCGTGGAGCAGGGCCGCCAACGAGGACGGCACCTTCAAGTCCGATGAGGAGT
TGGCCAAGCTTTACGCCGACGCCGGCCTAGACAACAGCAAGGAAACGATTGCCTACTGCCGAATCGGGGAACGGTCCTCG
CACACCTGGTTCGTGTTGCGGGAATTACTCGGACACCAAAACGTCAACATAGCATTTGGATATGGTCCACATGCTTGCCC
GGCCTCAGCGTATTCACGCATGTGCTTGACGACGTTCTTCACCTCGCTTACCCAGCGATTTCCGCAACTTCAACTCGCAA
GACCGTTTGAGGATTTGGAACGACGGGGTAAGGGCCTACATTCGGTGGGGATCAAGGAACTCCTTGTTACCTGGCCGACG
TGA

Upstream 100 bases:

>100_bases
AACGTGAAGCATCTCGACGGCGGTATCGCGGAGTGGACACGAACCATCGACTCCTCCTTGTTGGTGTACTAGCACCGAAC
TATGCGAAAGGATTCCCGCC

Downstream 100 bases:

>100_bases
CCCCGCGTGCCAGCAAGGGACTGTTGACTTCTCCGACGGATGAAAGCCGCCCTGGAATATCCAACCGCTCCTGCTCCTCG
GTCAACTCAAGCCGAAACCG

Product: putative thiosulfate sulfurtransferase cysA3

Products: NA

Alternate protein names: Rhodanese-like protein 2

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFVDAQQFSKLLSERGIANEDTV
ILYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDGRPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNL
IDVRSPDEFSGKILAPAHLPQEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSS
HTWFVLRELLGHQNVNIAFGYGPHACPASAYSRMCLTTFFTSLTQRFPQLQLARPFEDLERRGKGLHSVGIKELLVTWPT

Sequences:

>Translated_320_residues
MARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFVDAQQFSKLLSERGIANEDTV
ILYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDGRPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNL
IDVRSPDEFSGKILAPAHLPQEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSS
HTWFVLRELLGHQNVNIAFGYGPHACPASAYSRMCLTTFFTSLTQRFPQLQLARPFEDLERRGKGLHSVGIKELLVTWPT
>Mature_319_residues
ARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFVDAQQFSKLLSERGIANEDTVI
LYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDGRPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNLI
DVRSPDEFSGKILAPAHLPQEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSSH
TWFVLRELLGHQNVNIAFGYGPHACPASAYSRMCLTTFFTSLTQRFPQLQLARPFEDLERRGKGLHSVGIKELLVTWPT

Specific function: May be a sulfotransferase involved in the formation of thiosulfate

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains

Homologues:

Organism=Homo sapiens, GI17402865, Length=220, Percent_Identity=30, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI61835204, Length=257, Percent_Identity=26.8482490272374, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194473681, Length=257, Percent_Identity=26.8482490272374, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194473668, Length=257, Percent_Identity=26.8482490272374, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI87082121, Length=255, Percent_Identity=27.0588235294118, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17561888, Length=244, Percent_Identity=27.4590163934426, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71997283, Length=228, Percent_Identity=27.6315789473684, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI115534702, Length=209, Percent_Identity=29.6650717703349, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17543836, Length=220, Percent_Identity=26.8181818181818, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17543838, Length=220, Percent_Identity=26.8181818181818, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): THTR2_MYCBO (Q7TX80)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856789.1
- ProteinModelPortal:   Q7TX80
- SMR:   Q7TX80
- EnsemblBacteria:   EBMYCT00000016615
- GeneID:   1093127
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3144
- GeneTree:   EBGT00050000015573
- HOGENOM:   HBG709927
- OMA:   KLYGHQD
- ProtClustDB:   CLSK824808
- BioCyc:   MBOV233413:MB3144-MONOMER
- BRENDA:   2.8.1.1
- InterPro:   IPR001128
- InterPro:   IPR017972
- InterPro:   IPR001763
- Gene3D:   G3DSA:1.10.630.10
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF48264 Cytochrome_P450; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 35999; Mature: 35868

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00380 RHODANESE_1; PS00683 RHODANESE_2; PS50206 RHODANESE_3; PS00086 CYTOCHROME_P450

Important sites: ACT_SITE 233-233 BINDING 238-238

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFV
CCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHC
DAQQFSKLLSERGIANEDTVILYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDG
CHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEEEEEEECCCEEEEECCCCEEEEECC
RPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNLIDVRSPDEFSGKILAPAHLP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCEEECCCCCC
QEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSS
HHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
HTWFVLRELLGHQNVNIAFGYGPHACPASAYSRMCLTTFFTSLTQRFPQLQLARPFEDLE
CHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
RRGKGLHSVGIKELLVTWPT
HHCCCCHHCCHHHHEEECCC
>Mature Secondary Structure 
ARCDVLVSADWAESNLHAPKVVFVEVDEDTSAYDRDHIAGAIKLDWRTDLQDPVKRDFV
CCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHC
DAQQFSKLLSERGIANEDTVILYGGNNNWFAAYAYWYFKLYGHEKVKLLDGGRKKWELDG
CHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEEEEEEEECCCEEEEECCCCEEEEECC
RPLSSDPVSRPVTSYTASPPDNTIRAFRDEVLAAINVKNLIDVRSPDEFSGKILAPAHLP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCEEECCCCCC
QEQSQRPGHIPGAINVPWSRAANEDGTFKSDEELAKLYADAGLDNSKETIAYCRIGERSS
HHHCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
HTWFVLRELLGHQNVNIAFGYGPHACPASAYSRMCLTTFFTSLTQRFPQLQLARPFEDLE
CHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
RRGKGLHSVGIKELLVTWPT
HHCCCCHHCCHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972