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The map label for this gene is aspS [H]

Identifier: 121638457

GI number: 121638457

Start: 2860415

End: 2862250

Strand: Reverse

Name: aspS [H]

Synonym: BCG_2595c

Alternate gene names: 121638457

Gene position: 2862250-2860415 (Counterclockwise)

Preceding gene: 121638461

Following gene: 121638456

Centisome position: 65.43

GC content: 67.05

Gene sequence:

>1836_bases
GTGTTTGTGCTGCGCAGCCACGCCGCGGGTTTGCTACGGGAAGGCGACGCCGGGCAGCAGGTGACATTGGCCGGCTGGGT
GGCTCGCCGCCGCGACCACGGCGGCGTGATCTTCATCGATCTGCGGGACGCTTCGGGCATCGCACAGGTGGTGTTCCGCG
ACCCCCAGGACACTGAGGTGCTGGCGCAGGCTCACCGGCTGCGTGCGGAATTCTGCGTATCGGTGGCCGGCGTCGTCGAG
ATCCGCCCGGAAGGCAACGCCAACCCGGAGATCGCCACCGGCGAGATCGAGGTCAACGCCACGTCGTTGACCGTGCTGGG
CGAATGTGCGCCGCTGCCGTTTCAGCTGGACGAACCGGCGGGTGAGGAGTTGCGACTGAAGTACCGCTACCTCGATCTGC
GCCGCGACGACCCCGCTGCGGCGATTCGACTGCGTTCCCGGGTGAATGCCGCTGCGCGCGCGGTGCTGGCGCGTCACGAC
TTCGTCGAGATCGAGACGCCGACGATCACCCGCTCGACCCCGGAGGGAGCGCGCGACTTCCTGGTGCCGGCCCGACTGCA
CCCCGGTTCGTTTTACGCCCTACCGCAGAGCCCGCAGCTGTTCAAGCAGCTGCTGATGGTGGCGGGGATGGAACGCTACT
ACCAGATCGCCCGCTGCTACCGCGACGAGGACTTCCGCGCCGACCGCCAGCCCGAATTCACCCAGCTCGATATGGAGATG
AGCTTCGTCGACGCCGAGGACATCATCGCGATCTCCGAGGAAGTTCTGACCGAGCTGTGGGCGCTGATAGGCTACCGGAT
TCCGACGCCCATCCCGCGGATCGGCTATGCCGAAGCGATGCGCCGATTCGGTACGGACAAACCCGACCTGCGGTTCGGGC
TGGAGCTCGTCGAATGCACGGATTTCTTCTCCGACACCACATTTCGTGTCTTCCAGGCACCGTATGTGGGCGCGGTGGTG
ATGCCTGGCGGGGCGTCGCAGCCGCGGCGCACGCTGGACGGCTGGCAGGACTGGGCCAAGCAGCGCGGCCACCGCGGACT
GGCCTACGTGCTGGTCGCCGAAGACGGCACGCTGGGCGGTCCGGTGGCCAAAAACCTGACCGAGGCCGAGCGCACCGGAC
TGGCCGACCATGTCGGGGCCAAACCCGGCGATTGCATCTTCTTCTCGGCCGGTCCGGTCAAATCGTCGCGGGCACTGCTG
GGCGCGGCCCGCGTCGAGATCGCCAACCGGCTGGGCCTGATTGACCCCGATGCGTGGGCATTCGTCTGGGTCGTTGACCC
GCCGCTGTTCGAGCCGGCCGACGAAGCGACCGCCGCCGGTGAGGTCGCGGTCGGCTCGGGGGCCTGGACCGCGGTGCACC
ATGCCTTCACCGCGCCGAAACCGGAATGGGAGGACCGCATCGAATCCGATACCGGCAGCGTGCTGGCCGACGCGTACGAC
ATCGTCTGCAACGGCCACGAGATCGGTGGCGGCTCGGTGCGTATCCACCGCCGTGATATCCAGGAACGGGTGTTCGCGGT
GATGGGCCTGGACAAGGCCGAGGTGGAGGAAAAGTTCGGATTCCTGTTGGAGGCGTTCATGTTTGGCGCACCGCCGCACG
GCGGAATCGCGTTCGGGTGGGACCGGACCACCGCGCTGCTGGCCGGGATGGACTCGATCCGTGAGGTGATCGCGTTCCCG
AAGACCGGCGGCGGCGTCGATCCGCTCACCGACGCGCCGGCGCCCATTACCGCTCAGCAACGTAAGGAGTCCGGAATAGA
TGCCCAGCCCAAGCGGGTCCAGCGGCATGACCAGATCTTTGCGGTTACGACGTCGCACCCGAGTGTGAATAAATAG

Upstream 100 bases:

>100_bases
GTTGCAATGTTTGTTGAGATGAGCCGAAATTACCCTGCGTGACCGATGTGCGCCGCGGGCAGCTCTAGACTGAGCAGTCG
GTTAATCCGGTTGAAGGGGA

Downstream 100 bases:

>100_bases
GCGTTCATGAGCGCTTCGCTGCTAGTCAGGACGGCCTGCGGCGGCCGTGCGGTCGCCCAACGGTTGCGCACCGTGCTGTG
GCCGATCACCCAGACATCGG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEVLAQAHRLRAEFCVSVAGVVE
IRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPAGEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHD
FVEIETPTITRSTPEGARDFLVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECTDFFSDTTFRVFQAPYVGAVV
MPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGGPVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALL
GAARVEIANRLGLIDPDAWAFVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEVEEKFGFLLEAFMFGAPPHGGIAFGWDRTTALLAGMDSIREVIAFP
KTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQRHDQIFAVTTSHPSVNK

Sequences:

>Translated_611_residues
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEVLAQAHRLRAEFCVSVAGVVE
IRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPAGEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHD
FVEIETPTITRSTPEGARDFLVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECTDFFSDTTFRVFQAPYVGAVV
MPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGGPVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALL
GAARVEIANRLGLIDPDAWAFVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEVEEKFGFLLEAFMFGAPPHGGIAFGWDRTTALLAGMDSIREVIAFP
KTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQRHDQIFAVTTSHPSVNK
>Mature_611_residues
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEVLAQAHRLRAEFCVSVAGVVE
IRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPAGEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHD
FVEIETPTITRSTPEGARDFLVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECTDFFSDTTFRVFQAPYVGAVV
MPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGGPVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALL
GAARVEIANRLGLIDPDAWAFVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEVEEKFGFLLEAFMFGAPPHGGIAFGWDRTTALLAGMDSIREVIAFP
KTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQRHDQIFAVTTSHPSVNK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=606, Percent_Identity=39.6039603960396, Blast_Score=418, Evalue=1e-116,
Organism=Homo sapiens, GI45439306, Length=290, Percent_Identity=26.551724137931, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI194272210, Length=257, Percent_Identity=28.7937743190661, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI5031815, Length=257, Percent_Identity=28.7937743190661, Blast_Score=85, Evalue=3e-16,
Organism=Homo sapiens, GI4758762, Length=251, Percent_Identity=25.8964143426295, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788173, Length=581, Percent_Identity=46.815834767642, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=28.2758620689655, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789256, Length=289, Percent_Identity=26.9896193771626, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=610, Percent_Identity=34.2622950819672, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17551876, Length=286, Percent_Identity=26.9230769230769, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=294, Percent_Identity=25.1700680272109, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994340, Length=266, Percent_Identity=24.0601503759398, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=179, Percent_Identity=27.9329608938547, Blast_Score=73, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=266, Percent_Identity=24.0601503759398, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=639, Percent_Identity=31.924882629108, Blast_Score=282, Evalue=9e-77,
Organism=Saccharomyces cerevisiae, GI6321807, Length=264, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=274, Percent_Identity=27.7372262773723, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=263, Percent_Identity=25.8555133079848, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24584738, Length=605, Percent_Identity=38.5123966942149, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24640849, Length=274, Percent_Identity=27.7372262773723, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640851, Length=271, Percent_Identity=27.6752767527675, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921528, Length=294, Percent_Identity=25.8503401360544, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136276, Length=238, Percent_Identity=26.4705882352941, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66976; Mature: 66976

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEV
CEEECCCCCHHHCCCCCCCEEEEEHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHH
LAQAHRLRAEFCVSVAGVVEIRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPA
HHHHHHHHHHHHHEEEEEEEECCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCEECCCCC
GEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHDFVEIETPTITRSTPEGARDF
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCCCHHC
LVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
EEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECT
EEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
DFFSDTTFRVFQAPYVGAVVMPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGG
HHHCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALLGAARVEIANRLGLIDPDAWA
HHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
FVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
EEEEECCCCCCCCCHHCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHCEE
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEVEEKFGFLLEAFMFGAPPHGGIAFGW
EEECCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECC
DRTTALLAGMDSIREVIAFPKTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQRHDQIF
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHCCCCEE
AVTTSHPSVNK
EEEECCCCCCC
>Mature Secondary Structure
MFVLRSHAAGLLREGDAGQQVTLAGWVARRRDHGGVIFIDLRDASGIAQVVFRDPQDTEV
CEEECCCCCHHHCCCCCCCEEEEEHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCHHHH
LAQAHRLRAEFCVSVAGVVEIRPEGNANPEIATGEIEVNATSLTVLGECAPLPFQLDEPA
HHHHHHHHHHHHHEEEEEEEECCCCCCCCCEEEEEEEEEEEEEEEEECCCCCCEECCCCC
GEELRLKYRYLDLRRDDPAAAIRLRSRVNAAARAVLARHDFVEIETPTITRSTPEGARDF
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEECCCCCCCHHC
LVPARLHPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDMEM
EEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFVDAEDIIAISEEVLTELWALIGYRIPTPIPRIGYAEAMRRFGTDKPDLRFGLELVECT
EEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
DFFSDTTFRVFQAPYVGAVVMPGGASQPRRTLDGWQDWAKQRGHRGLAYVLVAEDGTLGG
HHHCCCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCC
PVAKNLTEAERTGLADHVGAKPGDCIFFSAGPVKSSRALLGAARVEIANRLGLIDPDAWA
HHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEE
FVWVVDPPLFEPADEATAAGEVAVGSGAWTAVHHAFTAPKPEWEDRIESDTGSVLADAYD
EEEEECCCCCCCCCHHCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHCCCCCHHHHHCEE
IVCNGHEIGGGSVRIHRRDIQERVFAVMGLDKAEVEEKFGFLLEAFMFGAPPHGGIAFGW
EEECCEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECC
DRTTALLAGMDSIREVIAFPKTGGGVDPLTDAPAPITAQQRKESGIDAQPKRVQRHDQIF
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHCCCCEE
AVTTSHPSVNK
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]