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The map label for this gene is plsB2 [H]

Identifier: 121638363

GI number: 121638363

Start: 2750723

End: 2753092

Strand: Reverse

Name: plsB2 [H]

Synonym: BCG_2500c

Alternate gene names: 121638363

Gene position: 2753092-2750723 (Counterclockwise)

Preceding gene: 121638364

Following gene: 121638362

Centisome position: 62.93

GC content: 64.39

Gene sequence:

>2370_bases
GTGACCAAACCGGCGGCCGATGCCAGCGCGGTGCTTACTGCCGAGGACACACTGGTGCTGGCTTCCACGGCGACGCCGGT
CGAGATGGAGCTGATCATGGGCTGGCTGGGCCAGCAGCGTGCACGCCATCCGGACTCGAAGTTCGACATATTGAAGCTGC
CACCGCGCAACGCTCCGCCGGCGGCGCTGACGGCACTGGTCGAGCAGCTCGAGCCCGGCTTCGCATCCAGCCCGCAATCT
GGCGAGGACCGTTCTATCGTGCCGGTTCGGGTGATCTGGCTGCCTCCCGCCGATCGCAGCCGGGCGGGCAAGGTGGCCGC
ACTGCTCCCGGGTCGGGATCCCTACCATCCCAGCCAGCGTCAGCAGCGTCGCATCCTGCGTACCGATCCCAGGCGCGCGC
GGGTGGTGGCCGGCGAGTCGGCCAAGGTGTCCGAACTGCGCCAGCAGTGGCGCGATACCACGGTGGCAGAGCACAAGCGC
GATTTCGCCCAGTTCGTCAGCCGCCGAGCGCTGTTGGCGCTGGCGCGCGCCGAATATCGGATCCTTGGACCGCAATACAA
ATCTCCCCGGCTGGTGAAGCCGGAGATGTTGGCGTCCGCACGATTTCGTGCCGGCCTGGACCGGATTCCGGGCGCCACGG
TCGAAGATGCCGGGAAGATGCTCGACGAACTCTCCACCGGATGGAGCCAGGTGTCGGTAGACCTGGTTTCCGTCCTCGGC
AGGCTGGCTAGCCGCGGCTTCGATCCGGAATTCGACTACGACGAGTATCAGGTCGCGGCGATGCGCGCCGCACTGGAGGC
TCATCCGGCGGTCCTGCTGTTCTCGCACCGGTCCTACATCGACGGCGTGGTGGTACCGGTGGCCATGCAGGACAACCGGT
TACCGCCGGTGCACATGTTCGGCGGCATCAACCTGTCGTTCGGTCTCATGGGACCCCTCATGCGGCGCTCGGGGATGATC
TTCATCCGGCGCAATATCGGCAACGACCCACTGTATAAGTACGTGCTCAAGGAGTACGTGGGCTACGTGGTCGAGAAGCG
GTTCAACCTGAGCTGGTCCATCGAAGGCACCCGGTCGCGCACCGGAAAGATGTTGCCGCCCAAGCTCGGTTTGATGAGCT
ACGTGGCCGATGCTTACCTGGACGGCCGCAGTGACGACATCCTGCTGCAGGGGGTTTCGATTTGCTTCGATCAGCTGCAC
GAGATCACCGAATACGCCGCCTACGCGCGTGGCGCGGAGAAGACGCCCGAAGGTTTGCGCTGGCTCTACAACTTCATCAA
GGCGCAGGGGGAACGCAACTTCGGCAAGATCTACGTTCGCTTCCCCGAAGCGGTCTCGATGCGCCAGTACCTCGGCGCAC
CGCACGGCGAGCTGACCCAGGATCCGGCCGCGAAACGGCTTGCGTTGCAGAAGATGTCGTTCGAGGTGGCCTGGAGGATT
TTGCAGGCGACGCCGGTGACCGCGACGGGTTTGGTGTCCGCACTGCTGCTCACCACCCGCGGCACCGCGTTGACGCTCGA
CCAGCTGCACCACACGTTGCAGGACTCACTGGACTATCTGGAACGCAAACAATCGCCGGTTTCGACAAGCGCATTGCGAC
TGCGCTCGCGCGAAGGCGTCCGTGCGGCGGCGGACGCGTTGTCCAACGGCCACCCGGTCACTCGGGTCGACAGTGGCCGG
GAGCCGGTATGGTACATAGCGCCTGACGACGAGCACGCCGCGGCGTTCTACCGGAACTCGGTGATCCATGCGTTTTTGGA
GACCTCGATCGTCGAGCTCGCGCTGGCCCATGCCAAGCACGCCGAAGGTGACCGCGTCGCCGCGTTCTGGGCCCAGGCGA
TGCGGTTGCGGGATCTGCTGAAGTTCGACTTCTATTTCGCGGATTCCACGGCGTTTCGGGCCAACATCGCCCAAGAGATG
GCCTGGCACCAAGACTGGGAGGATCATCTTGGCGTCGGGGGCAATGAGATCGACGCGATGCTGTATGCCAAACGGCCGCT
GATGTCGGACGCGATGTTGCGGGTCTTCTTCGAAGCCTATGAGATCGTTGCCGACGTGTTGCGCGATGCTCCGCCTGACA
TCGGTCCTGAGGAGTTGACGGAGCTGGCGCTCGGCCTCGGCCGTCAGTTTGTGGCACAGGGCCGGGTCCGCAGCAGCGAA
CCGGTATCGACGCTGCTGTTCGCCACTGCACGCCAGGTCGCCGTCGATCAGGAGCTGATAGCGCCGGCGGCCGACCTCGC
CGAACGTAGGGTCGCCTTCCGGCGGGAGTTACGAAACATTCTGCGGGATTTCGACTATGTCGAGCAGATCGCGCGCAACC
AGTTCGTCGCCCGCGAGTTCAAAGCGCGTCAAGGACGCGACCGAATCTAA

Upstream 100 bases:

>100_bases
CAAAGAAGGCATCGCCGAAGGCCAAACCCAGCGAATCTAAGATCGCTGGCAAGGATGGTGAGGCCTCCGCATCGCCAAGC
TCTAGCGCCAAAGGACGGTC

Downstream 100 bases:

>100_bases
GAACGTGTTGGGGGCGTGGCGTTGCGTAGACGCCATGAGCCAGACGGCTGGCCATTTTCCCAGCGCTCAGAAAAACCGAA
TGCGGTGCGCCATGCCGTCA

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 789; Mature: 788

Protein sequence:

>789_residues
MTKPAADASAVLTAEDTLVLASTATPVEMELIMGWLGQQRARHPDSKFDILKLPPRNAPPAALTALVEQLEPGFASSPQS
GEDRSIVPVRVIWLPPADRSRAGKVAALLPGRDPYHPSQRQQRRILRTDPRRARVVAGESAKVSELRQQWRDTTVAEHKR
DFAQFVSRRALLALARAEYRILGPQYKSPRLVKPEMLASARFRAGLDRIPGATVEDAGKMLDELSTGWSQVSVDLVSVLG
RLASRGFDPEFDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGVVVPVAMQDNRLPPVHMFGGINLSFGLMGPLMRRSGMI
FIRRNIGNDPLYKYVLKEYVGYVVEKRFNLSWSIEGTRSRTGKMLPPKLGLMSYVADAYLDGRSDDILLQGVSICFDQLH
EITEYAAYARGAEKTPEGLRWLYNFIKAQGERNFGKIYVRFPEAVSMRQYLGAPHGELTQDPAAKRLALQKMSFEVAWRI
LQATPVTATGLVSALLLTTRGTALTLDQLHHTLQDSLDYLERKQSPVSTSALRLRSREGVRAAADALSNGHPVTRVDSGR
EPVWYIAPDDEHAAAFYRNSVIHAFLETSIVELALAHAKHAEGDRVAAFWAQAMRLRDLLKFDFYFADSTAFRANIAQEM
AWHQDWEDHLGVGGNEIDAMLYAKRPLMSDAMLRVFFEAYEIVADVLRDAPPDIGPEELTELALGLGRQFVAQGRVRSSE
PVSTLLFATARQVAVDQELIAPAADLAERRVAFRRELRNILRDFDYVEQIARNQFVAREFKARQGRDRI

Sequences:

>Translated_789_residues
MTKPAADASAVLTAEDTLVLASTATPVEMELIMGWLGQQRARHPDSKFDILKLPPRNAPPAALTALVEQLEPGFASSPQS
GEDRSIVPVRVIWLPPADRSRAGKVAALLPGRDPYHPSQRQQRRILRTDPRRARVVAGESAKVSELRQQWRDTTVAEHKR
DFAQFVSRRALLALARAEYRILGPQYKSPRLVKPEMLASARFRAGLDRIPGATVEDAGKMLDELSTGWSQVSVDLVSVLG
RLASRGFDPEFDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGVVVPVAMQDNRLPPVHMFGGINLSFGLMGPLMRRSGMI
FIRRNIGNDPLYKYVLKEYVGYVVEKRFNLSWSIEGTRSRTGKMLPPKLGLMSYVADAYLDGRSDDILLQGVSICFDQLH
EITEYAAYARGAEKTPEGLRWLYNFIKAQGERNFGKIYVRFPEAVSMRQYLGAPHGELTQDPAAKRLALQKMSFEVAWRI
LQATPVTATGLVSALLLTTRGTALTLDQLHHTLQDSLDYLERKQSPVSTSALRLRSREGVRAAADALSNGHPVTRVDSGR
EPVWYIAPDDEHAAAFYRNSVIHAFLETSIVELALAHAKHAEGDRVAAFWAQAMRLRDLLKFDFYFADSTAFRANIAQEM
AWHQDWEDHLGVGGNEIDAMLYAKRPLMSDAMLRVFFEAYEIVADVLRDAPPDIGPEELTELALGLGRQFVAQGRVRSSE
PVSTLLFATARQVAVDQELIAPAADLAERRVAFRRELRNILRDFDYVEQIARNQFVAREFKARQGRDRI
>Mature_788_residues
TKPAADASAVLTAEDTLVLASTATPVEMELIMGWLGQQRARHPDSKFDILKLPPRNAPPAALTALVEQLEPGFASSPQSG
EDRSIVPVRVIWLPPADRSRAGKVAALLPGRDPYHPSQRQQRRILRTDPRRARVVAGESAKVSELRQQWRDTTVAEHKRD
FAQFVSRRALLALARAEYRILGPQYKSPRLVKPEMLASARFRAGLDRIPGATVEDAGKMLDELSTGWSQVSVDLVSVLGR
LASRGFDPEFDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGVVVPVAMQDNRLPPVHMFGGINLSFGLMGPLMRRSGMIF
IRRNIGNDPLYKYVLKEYVGYVVEKRFNLSWSIEGTRSRTGKMLPPKLGLMSYVADAYLDGRSDDILLQGVSICFDQLHE
ITEYAAYARGAEKTPEGLRWLYNFIKAQGERNFGKIYVRFPEAVSMRQYLGAPHGELTQDPAAKRLALQKMSFEVAWRIL
QATPVTATGLVSALLLTTRGTALTLDQLHHTLQDSLDYLERKQSPVSTSALRLRSREGVRAAADALSNGHPVTRVDSGRE
PVWYIAPDDEHAAAFYRNSVIHAFLETSIVELALAHAKHAEGDRVAAFWAQAMRLRDLLKFDFYFADSTAFRANIAQEMA
WHQDWEDHLGVGGNEIDAMLYAKRPLMSDAMLRVFFEAYEIVADVLRDAPPDIGPEELTELALGLGRQFVAQGRVRSSEP
VSTLLFATARQVAVDQELIAPAADLAERRVAFRRELRNILRDFDYVEQIARNQFVAREFKARQGRDRI

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=535, Percent_Identity=26.1682242990654, Blast_Score=115, Evalue=2e-25,
Organism=Homo sapiens, GI190358539, Length=390, Percent_Identity=22.8205128205128, Blast_Score=84, Evalue=6e-16,
Organism=Escherichia coli, GI87082362, Length=469, Percent_Identity=26.865671641791, Blast_Score=135, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71988723, Length=514, Percent_Identity=22.7626459143969, Blast_Score=101, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988728, Length=426, Percent_Identity=23.0046948356808, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25147672, Length=252, Percent_Identity=29.3650793650794, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17864692, Length=348, Percent_Identity=25.2873563218391, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24650752, Length=374, Percent_Identity=25.9358288770053, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24650754, Length=374, Percent_Identity=25.9358288770053, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21357731, Length=374, Percent_Identity=25.9358288770053, Blast_Score=97, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 88369; Mature: 88238

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPAADASAVLTAEDTLVLASTATPVEMELIMGWLGQQRARHPDSKFDILKLPPRNAPP
CCCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCH
AALTALVEQLEPGFASSPQSGEDRSIVPVRVIWLPPADRSRAGKVAALLPGRDPYHPSQR
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCHH
QQRRILRTDPRRARVVAGESAKVSELRQQWRDTTVAEHKRDFAQFVSRRALLALARAEYR
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGPQYKSPRLVKPEMLASARFRAGLDRIPGATVEDAGKMLDELSTGWSQVSVDLVSVLG
EECCCCCCCCCCCHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RLASRGFDPEFDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGVVVPVAMQDNRLPPVHMF
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHCCEEEEEEECCCCCCCEEEE
GGINLSFGLMGPLMRRSGMIFIRRNIGNDPLYKYVLKEYVGYVVEKRFNLSWSIEGTRSR
CCCEECHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
TGKMLPPKLGLMSYVADAYLDGRSDDILLQGVSICFDQLHEITEYAAYARGAEKTPEGLR
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
WLYNFIKAQGERNFGKIYVRFPEAVSMRQYLGAPHGELTQDPAAKRLALQKMSFEVAWRI
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LQATPVTATGLVSALLLTTRGTALTLDQLHHTLQDSLDYLERKQSPVSTSALRLRSREGV
HHCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCH
RAAADALSNGHPVTRVDSGREPVWYIAPDDEHAAAFYRNSVIHAFLETSIVELALAHAKH
HHHHHHHCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEGDRVAAFWAQAMRLRDLLKFDFYFADSTAFRANIAQEMAWHQDWEDHLGVGGNEIDAM
CCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHH
LYAKRPLMSDAMLRVFFEAYEIVADVLRDAPPDIGPEELTELALGLGRQFVAQGRVRSSE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PVSTLLFATARQVAVDQELIAPAADLAERRVAFRRELRNILRDFDYVEQIARNQFVAREF
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KARQGRDRI
HHHCCCCCC
>Mature Secondary Structure 
TKPAADASAVLTAEDTLVLASTATPVEMELIMGWLGQQRARHPDSKFDILKLPPRNAPP
CCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCH
AALTALVEQLEPGFASSPQSGEDRSIVPVRVIWLPPADRSRAGKVAALLPGRDPYHPSQR
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCHH
QQRRILRTDPRRARVVAGESAKVSELRQQWRDTTVAEHKRDFAQFVSRRALLALARAEYR
HHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGPQYKSPRLVKPEMLASARFRAGLDRIPGATVEDAGKMLDELSTGWSQVSVDLVSVLG
EECCCCCCCCCCCHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
RLASRGFDPEFDYDEYQVAAMRAALEAHPAVLLFSHRSYIDGVVVPVAMQDNRLPPVHMF
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHCCEEEEEEECCCCCCCEEEE
GGINLSFGLMGPLMRRSGMIFIRRNIGNDPLYKYVLKEYVGYVVEKRFNLSWSIEGTRSR
CCCEECHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
TGKMLPPKLGLMSYVADAYLDGRSDDILLQGVSICFDQLHEITEYAAYARGAEKTPEGLR
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
WLYNFIKAQGERNFGKIYVRFPEAVSMRQYLGAPHGELTQDPAAKRLALQKMSFEVAWRI
HHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LQATPVTATGLVSALLLTTRGTALTLDQLHHTLQDSLDYLERKQSPVSTSALRLRSREGV
HHCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCH
RAAADALSNGHPVTRVDSGREPVWYIAPDDEHAAAFYRNSVIHAFLETSIVELALAHAKH
HHHHHHHCCCCCEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEGDRVAAFWAQAMRLRDLLKFDFYFADSTAFRANIAQEMAWHQDWEDHLGVGGNEIDAM
CCCCHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHH
LYAKRPLMSDAMLRVFFEAYEIVADVLRDAPPDIGPEELTELALGLGRQFVAQGRVRSSE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
PVSTLLFATARQVAVDQELIAPAADLAERRVAFRRELRNILRDFDYVEQIARNQFVAREF
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KARQGRDRI
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]