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The map label for this gene is mbtG

Identifier: 121638257

GI number: 121638257

Start: 2621430

End: 2622725

Strand: Reverse

Name: mbtG

Synonym: BCG_2392c

Alternate gene names: 121638257

Gene position: 2622725-2621430 (Counterclockwise)

Preceding gene: 121638258

Following gene: 121638256

Centisome position: 59.95

GC content: 66.51

Gene sequence:

>1296_bases
ATGAATCCGACGCTCGCGGTCCTGGGCGCTGGAGCCAAGGCGGTGGCGGTCGCGGCCAAGGCATCCGTGCTGCGTGACAT
GGGGGTCGACGTGCCCGACGTGATCGCCGTCGAACGCATCGGGGTCGGGGCCAACTGGCAGGCCAGCGGTGGCTGGACCG
ACGGAGCCCACCGGCTGGGCACCAGCCCAGAAAAGGATGTCGGTTTTCCCTACCGGTCGGCGCTGGTGCCACGGCGCAAC
GCAGAATTGGACGAGCGGATGACCCGCTACAGCTGGCAGTCGTATCTGATCGCCACCGCGTCGTTCGCGGAATGGATCGA
CCGGGGCCGCCCGGCGCCCACCCATCGCAGGTGGAGTCAGTACCTAGCCTGGGTGGCCGATCACATTGGCCTCAAGGTGA
TCCACGGCGAGGTCGAACGGCTCGCCGTCACCGGTGACCGCTGGGCGTTGTGCACCCACGAGACCACCGTGCAGGCCGAC
GCGTTGATGATCACCGGGCCCGGCCAGGCTGAAAAGTCGCTACTGCCCGGAAACCCGCGCGTGCTCTCAATCGCACAGTT
CTGGGACCGTGCCGCCGGCCACGACCGGATCAACGCCGAGCGGGTCGCGGTGATCGGTGGCGGAGAGACGGCCGCATCGA
TGCTCAACGAGCTGTTCCGGCATCGGGTCTCAACCATCACCGTCATCTCCCCGCAGGTAACCCTGTTCACCCGCGGCGAG
GGATTCTTCGAGAACTCACTGTTTTCCGATCCGACCGACTGGGCGGCCTTGACGTTCGACGAACGGCGCGACGCGCTGGC
CCGCACCGACCGAGGAGTGTTCTCGGCGACCGTGCAGGAAGCGCTGCTGGCCGATGACCGCATCCATCATCTGCGTGGCC
GGGTCGCCCACGCGGTGGGCCGTCAGGGGCAGATCCGGTTGACGCTGAGCACCAACCGGGGCAGCGAGAACTTCGAGACC
GTGCACGGATTCGATCTCGTCATCGACGGCTCGGGCGCCGATCCGCTGTGGTTCACCTCACTGTTCAGTCAGCACACCCT
CGACCTGCTCGAGCTGGGACTGGGTGGACCGCTGACCGCCGACCGCCTGCAGGAAGCGATCGGCTACGACTTGGCAGTCA
CCGACGTCACGCCCAAGCTGTTCCTGCCCACCCTGTCCGGACTCACCCAGGGGCCCGGGTTCCCCAACCTGAGCTGCCTC
GGCTTGTTGTCGGACCGGGTGCTCGGCGCCGGCATCTTTACGCCGACCAAACACAACGACACAAGGAGAAGCGGTGAGCA
CCAATCCTTTCGATGA

Upstream 100 bases:

>100_bases
TGCTAGCCACGCGGTGGCGCACGCTGCCCGACATCCTGTCCGCCGACGACGTCGCCACGCTGCAATCACTGTGGCAGGGC
GCGCTGGCGGAGATAACAGC

Downstream 100 bases:

>100_bases
CGACAACGGCGCATTCTTCGTGCTGGTCAACGACGAAGACCAGCACAGCCTGTGGCCGGTGTTCGCCGATATCCCGGCCG
GCTGGCGCGTGGTGCACGGC

Product: lysine-N-oxygenase mbtG

Products: NA

Alternate protein names: Lysine 6-N-hydroxylase; Lysine N(6)-hydroxylase; Lysine-N-oxygenase; Mycobactin synthase protein G

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNPTLAVLGAGAKAVAVAAKASVLRDMGVDVPDVIAVERIGVGANWQASGGWTDGAHRLGTSPEKDVGFPYRSALVPRRN
AELDERMTRYSWQSYLIATASFAEWIDRGRPAPTHRRWSQYLAWVADHIGLKVIHGEVERLAVTGDRWALCTHETTVQAD
ALMITGPGQAEKSLLPGNPRVLSIAQFWDRAAGHDRINAERVAVIGGGETAASMLNELFRHRVSTITVISPQVTLFTRGE
GFFENSLFSDPTDWAALTFDERRDALARTDRGVFSATVQEALLADDRIHHLRGRVAHAVGRQGQIRLTLSTNRGSENFET
VHGFDLVIDGSGADPLWFTSLFSQHTLDLLELGLGGPLTADRLQEAIGYDLAVTDVTPKLFLPTLSGLTQGPGFPNLSCL
GLLSDRVLGAGIFTPTKHNDTRRSGEHQSFR

Sequences:

>Translated_431_residues
MNPTLAVLGAGAKAVAVAAKASVLRDMGVDVPDVIAVERIGVGANWQASGGWTDGAHRLGTSPEKDVGFPYRSALVPRRN
AELDERMTRYSWQSYLIATASFAEWIDRGRPAPTHRRWSQYLAWVADHIGLKVIHGEVERLAVTGDRWALCTHETTVQAD
ALMITGPGQAEKSLLPGNPRVLSIAQFWDRAAGHDRINAERVAVIGGGETAASMLNELFRHRVSTITVISPQVTLFTRGE
GFFENSLFSDPTDWAALTFDERRDALARTDRGVFSATVQEALLADDRIHHLRGRVAHAVGRQGQIRLTLSTNRGSENFET
VHGFDLVIDGSGADPLWFTSLFSQHTLDLLELGLGGPLTADRLQEAIGYDLAVTDVTPKLFLPTLSGLTQGPGFPNLSCL
GLLSDRVLGAGIFTPTKHNDTRRSGEHQSFR
>Mature_431_residues
MNPTLAVLGAGAKAVAVAAKASVLRDMGVDVPDVIAVERIGVGANWQASGGWTDGAHRLGTSPEKDVGFPYRSALVPRRN
AELDERMTRYSWQSYLIATASFAEWIDRGRPAPTHRRWSQYLAWVADHIGLKVIHGEVERLAVTGDRWALCTHETTVQAD
ALMITGPGQAEKSLLPGNPRVLSIAQFWDRAAGHDRINAERVAVIGGGETAASMLNELFRHRVSTITVISPQVTLFTRGE
GFFENSLFSDPTDWAALTFDERRDALARTDRGVFSATVQEALLADDRIHHLRGRVAHAVGRQGQIRLTLSTNRGSENFET
VHGFDLVIDGSGADPLWFTSLFSQHTLDLLELGLGGPLTADRLQEAIGYDLAVTDVTPKLFLPTLSGLTQGPGFPNLSCL
GLLSDRVLGAGIFTPTKHNDTRRSGEHQSFR

Specific function: Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (no

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MBTG_MYCBO (Q7TYQ9)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856048.1
- ProteinModelPortal:   Q7TYQ9
- EnsemblBacteria:   EBMYCT00000014929
- GeneID:   1094387
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2399c
- GeneTree:   EBGT00050000017267
- HOGENOM:   HBG432039
- OMA:   SIAQFWH
- ProtClustDB:   CLSK791811
- BioCyc:   MBOV233413:MB2399C-MONOMER
- BRENDA:   1.14.13.59
- GO:   GO:0005488

Pfam domain/function: NA

EC number: =1.14.13.59

Molecular weight: Translated: 46944; Mature: 46944

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTLAVLGAGAKAVAVAAKASVLRDMGVDVPDVIAVERIGVGANWQASGGWTDGAHRLG
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCC
TSPEKDVGFPYRSALVPRRNAELDERMTRYSWQSYLIATASFAEWIDRGRPAPTHRRWSQ
CCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
YLAWVADHIGLKVIHGEVERLAVTGDRWALCTHETTVQADALMITGPGQAEKSLLPGNPR
HHHHHHHHCCEEEEECCEEEEEECCCCEEEEECCCEEEECEEEEECCCCHHCCCCCCCCC
VLSIAQFWDRAAGHDRINAERVAVIGGGETAASMLNELFRHRVSTITVISPQVTLFTRGE
EEEHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCEEEEECCEEEEEECCC
GFFENSLFSDPTDWAALTFDERRDALARTDRGVFSATVQEALLADDRIHHLRGRVAHAVG
CCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RQGQIRLTLSTNRGSENFETVHGFDLVIDGSGADPLWFTSLFSQHTLDLLELGLGGPLTA
CCCEEEEEEECCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
DRLQEAIGYDLAVTDVTPKLFLPTLSGLTQGPGFPNLSCLGLLSDRVLGAGIFTPTKHND
HHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
TRRSGEHQSFR
CCCCCCCCCCC
>Mature Secondary Structure
MNPTLAVLGAGAKAVAVAAKASVLRDMGVDVPDVIAVERIGVGANWQASGGWTDGAHRLG
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCC
TSPEKDVGFPYRSALVPRRNAELDERMTRYSWQSYLIATASFAEWIDRGRPAPTHRRWSQ
CCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
YLAWVADHIGLKVIHGEVERLAVTGDRWALCTHETTVQADALMITGPGQAEKSLLPGNPR
HHHHHHHHCCEEEEECCEEEEEECCCCEEEEECCCEEEECEEEEECCCCHHCCCCCCCCC
VLSIAQFWDRAAGHDRINAERVAVIGGGETAASMLNELFRHRVSTITVISPQVTLFTRGE
EEEHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCEEEEECCEEEEEECCC
GFFENSLFSDPTDWAALTFDERRDALARTDRGVFSATVQEALLADDRIHHLRGRVAHAVG
CCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RQGQIRLTLSTNRGSENFETVHGFDLVIDGSGADPLWFTSLFSQHTLDLLELGLGGPLTA
CCCEEEEEEECCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
DRLQEAIGYDLAVTDVTPKLFLPTLSGLTQGPGFPNLSCLGLLSDRVLGAGIFTPTKHND
HHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
TRRSGEHQSFR
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972