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The map label for this gene is amiA2

Identifier: 121638242

GI number: 121638242

Start: 2609342

End: 2610796

Strand: Direct

Name: amiA2

Synonym: BCG_2377

Alternate gene names: 121638242

Gene position: 2609342-2610796 (Clockwise)

Preceding gene: 121638238

Following gene: 121638250

Centisome position: 59.65

GC content: 67.97

Gene sequence:

>1455_bases
GTGGTTGGCGCTTCTGGGTCGGATGCTGGAGCCATTTCCGGCTCTGGCAACCAGCGCCTGCCCACCCTGACCGACCTGCT
CTACCAGCTGGCCACCCGCGCAGTGACGTCCGAAGAGTTGGTGCGACGTTCCCTGCGCGCGATCGATGTGAGCCAGCCCA
CATTGAACGCCTTCCGGGTAGTGCTCACCGAATCCGCGCTGGCCGACGCGGCGGCCGCCGATAAGCGGCGGGCGGCCGGC
GACACGGCGCCGCTGCTGGGCATTCCGATCGCGGTCAAGGACGACGTCGACGTTGCTGGAGTGCCAACCGCCTTCGGCAC
CCAGGGCTATGTCGCGCCTGCTACCGACGACTGTGAGGTCGTCCGGCGCCTCAAGGCGGCCGGAGCGGTGATCGTCGGCA
AGACGAATACTTGTGAATTGGGCCAGTGGCCGTTCACCAGCGGACCCGGGTTCGGACACACCCGCAACCCCTGGTCGCGC
CGGCACACGCCGGGTGGATCCTCGGGCGGTAGCGCGGCGGCGGTGGCCGCCGGCCTGGTTACCGCCGCTATCGGCTCCGA
CGGCGCCGGCAGCATCCGCATCCCCGCAGCATGGACACACCTAGTGGGCATCAAGCCACAACGCGGTCGGATCTCCACCT
GGCCGCTGCCGGAGGCGTTCAACGGCGTCACGGTCAACGGCGTACTGGCCCGCACTGTGGAGGATGCGGCGCTGGTGCTG
GACGCCGCGTCCGGCAACGTCGAGGGCGACCGCCACCAGCCACCCCCGGTGACGGTGTCCGATTTCGTCGGCATCGCCCC
TGGACCGCTGAAGATTGCCTTGTCAACCCACTTCCCGTACACCGGCTTTCGGGCCAAGTTGCATCCTGAGATCTTGGCCG
CGACCCAGAGGGTGGGCGACCAGCTCGAGCTGCTCGGCCATACGGTGGTGAAAGGCAATCCGGACTACGGCCTACGGTTG
TCGTGGAACTTTCTTGCCCGGTCCACCGCGGGCCTCTGGGAATGGGCGGAGCGGCTAGGCGACGAGGTGACCCTGGATCG
TCGCACCGTATCCAACCTGCGCATGGGGCACGTGCTGTCGCAGGCGATTCTGCGCAGCGCGCGCCGCCACGAAGCCGCCG
ACCAGCGTCGGGTCGGCTCGATCTTCGACATCGTCGACGTGGTGCTGGCACCGACCACAGCACAACCACCGCCAATGGCG
CGCGCGTTTGACCGGTTGGGCAGCTTCGGCACCGATCGCGCCATCATCGCCGCGTGCCCGTCGACCTGGCCGTGGAACCT
GCTGGGCTGGCCGTCGATCAATGTGCCGGCGGGGTTCACCTCCGACGGTTTGCCGATCGGTGTGCAACTGATGGGACCGG
CCAACAGCGAGGGCATGCTGATCTCGCTGGCCGCCGAGTTGGAAGCCGTCAGTGGCTGGGCGACCAAGCAGCCGCAGGTG
TGGTGGACGAGCTAA

Upstream 100 bases:

>100_bases
CTGGCGCAGCACAACAGCCCGGTCCCGATACAGCCGCATCACAATAGTTTTGCACCCCGCCACGACATCGCGGGTATCCG
CGCCGATAGTCTCGTACCCC

Downstream 100 bases:

>100_bases
AACCCCAGTCGGCCAAGCTGTTTGGGGTCGCGCTGCCAGTTCTTGGCGACCTTGACCCGCAAGTCGAGATAGACCTTGGT
GCCCAGCAGGTTTTCGATCT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MVGASGSDAGAISGSGNQRLPTLTDLLYQLATRAVTSEELVRRSLRAIDVSQPTLNAFRVVLTESALADAAAADKRRAAG
DTAPLLGIPIAVKDDVDVAGVPTAFGTQGYVAPATDDCEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSR
RHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGVTVNGVLARTVEDAALVL
DAASGNVEGDRHQPPPVTVSDFVGIAPGPLKIALSTHFPYTGFRAKLHPEILAATQRVGDQLELLGHTVVKGNPDYGLRL
SWNFLARSTAGLWEWAERLGDEVTLDRRTVSNLRMGHVLSQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQPPPMA
RAFDRLGSFGTDRAIIAACPSTWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWATKQPQV
WWTS

Sequences:

>Translated_484_residues
MVGASGSDAGAISGSGNQRLPTLTDLLYQLATRAVTSEELVRRSLRAIDVSQPTLNAFRVVLTESALADAAAADKRRAAG
DTAPLLGIPIAVKDDVDVAGVPTAFGTQGYVAPATDDCEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSR
RHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGVTVNGVLARTVEDAALVL
DAASGNVEGDRHQPPPVTVSDFVGIAPGPLKIALSTHFPYTGFRAKLHPEILAATQRVGDQLELLGHTVVKGNPDYGLRL
SWNFLARSTAGLWEWAERLGDEVTLDRRTVSNLRMGHVLSQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQPPPMA
RAFDRLGSFGTDRAIIAACPSTWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWATKQPQV
WWTS
>Mature_484_residues
MVGASGSDAGAISGSGNQRLPTLTDLLYQLATRAVTSEELVRRSLRAIDVSQPTLNAFRVVLTESALADAAAADKRRAAG
DTAPLLGIPIAVKDDVDVAGVPTAFGTQGYVAPATDDCEVVRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSR
RHTPGGSSGGSAAAVAAGLVTAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGVTVNGVLARTVEDAALVL
DAASGNVEGDRHQPPPVTVSDFVGIAPGPLKIALSTHFPYTGFRAKLHPEILAATQRVGDQLELLGHTVVKGNPDYGLRL
SWNFLARSTAGLWEWAERLGDEVTLDRRTVSNLRMGHVLSQAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQPPPMA
RAFDRLGSFGTDRAIIAACPSTWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWATKQPQV
WWTS

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=28.5148514851485, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI195972892, Length=494, Percent_Identity=25.9109311740891, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=240, Percent_Identity=34.5833333333333, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=398, Percent_Identity=27.3869346733668, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=247, Percent_Identity=29.9595141700405, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=455, Percent_Identity=27.4725274725275, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=168, Percent_Identity=38.0952380952381, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=168, Percent_Identity=38.0952380952381, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=464, Percent_Identity=23.9224137931034, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=446, Percent_Identity=28.2511210762332, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=231, Percent_Identity=31.1688311688312, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648113, Length=498, Percent_Identity=31.7269076305221, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24644968, Length=512, Percent_Identity=25.9765625, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078093, Length=512, Percent_Identity=27.5390625, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648435, Length=487, Percent_Identity=25.4620123203285, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648437, Length=487, Percent_Identity=25.4620123203285, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45550774, Length=487, Percent_Identity=25.4620123203285, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356731, Length=196, Percent_Identity=32.6530612244898, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648441, Length=422, Percent_Identity=25.1184834123223, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648439, Length=422, Percent_Identity=25.1184834123223, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652985, Length=260, Percent_Identity=29.6153846153846, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922090, Length=260, Percent_Identity=29.6153846153846, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652981, Length=260, Percent_Identity=29.6153846153846, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24652983, Length=260, Percent_Identity=29.6153846153846, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMIA2_MYCBO (P63491)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_856033.1
- ProteinModelPortal:   P63491
- EnsemblBacteria:   EBMYCT00000017780
- GeneID:   1094372
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2384
- GeneTree:   EBGT00050000014631
- HOGENOM:   HBG481888
- OMA:   WDFLARS
- ProtClustDB:   PRK06061
- BioCyc:   MBOV233413:MB2384-MONOMER
- BRENDA:   3.5.1.4
- InterPro:   IPR000120
- InterPro:   IPR020556
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: =3.5.1.4

Molecular weight: Translated: 50884; Mature: 50884

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 93-93 ACT_SITE 167-167 ACT_SITE 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGASGSDAGAISGSGNQRLPTLTDLLYQLATRAVTSEELVRRSLRAIDVSQPTLNAFRV
CCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
VLTESALADAAAADKRRAAGDTAPLLGIPIAVKDDVDVAGVPTAFGTQGYVAPATDDCEV
HHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCCCCCCCCCCCCCEECCCCCHHHH
VRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSRRHTPGGSSGGSAAAVAAGLV
HHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHH
TAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGVTVNGVLARTVEDAALVL
HHHCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHCCEEEEE
DAASGNVEGDRHQPPPVTVSDFVGIAPGPLKIALSTHFPYTGFRAKLHPEILAATQRVGD
ECCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHHH
QLELLGHTVVKGNPDYGLRLSWNFLARSTAGLWEWAERLGDEVTLDRRTVSNLRMGHVLS
HHHHHCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHH
QAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQPPPMARAFDRLGSFGTDRAIIAACP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECC
STWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWATKQPQV
CCCCCCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHCCCCCCCCEE
WWTS
EECC
>Mature Secondary Structure
MVGASGSDAGAISGSGNQRLPTLTDLLYQLATRAVTSEELVRRSLRAIDVSQPTLNAFRV
CCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHH
VLTESALADAAAADKRRAAGDTAPLLGIPIAVKDDVDVAGVPTAFGTQGYVAPATDDCEV
HHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCCCCCCCCCCCCCEECCCCCHHHH
VRRLKAAGAVIVGKTNTCELGQWPFTSGPGFGHTRNPWSRRHTPGGSSGGSAAAVAAGLV
HHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHH
TAAIGSDGAGSIRIPAAWTHLVGIKPQRGRISTWPLPEAFNGVTVNGVLARTVEDAALVL
HHHCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHCCEEEEE
DAASGNVEGDRHQPPPVTVSDFVGIAPGPLKIALSTHFPYTGFRAKLHPEILAATQRVGD
ECCCCCCCCCCCCCCCCCHHHHCCCCCCCEEEEEECCCCCCCCEEECCHHHHHHHHHHHH
QLELLGHTVVKGNPDYGLRLSWNFLARSTAGLWEWAERLGDEVTLDRRTVSNLRMGHVLS
HHHHHCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHH
QAILRSARRHEAADQRRVGSIFDIVDVVLAPTTAQPPPMARAFDRLGSFGTDRAIIAACP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECC
STWPWNLLGWPSINVPAGFTSDGLPIGVQLMGPANSEGMLISLAAELEAVSGWATKQPQV
CCCCCCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEHHHHHHCCCCCCCCEE
WWTS
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972