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The map label for this gene is pks16

Identifier: 121636942

GI number: 121636942

Start: 1162115

End: 1163749

Strand: Direct

Name: pks16

Synonym: BCG_1070

Alternate gene names: 121636942

Gene position: 1162115-1163749 (Clockwise)

Preceding gene: 121636941

Following gene: 121636948

Centisome position: 26.57

GC content: 66.24

Gene sequence:

>1635_bases
TTGAGCAGGTTCACCGAGAAGATGTTCCACAATGCCCGCACCGCGACGACGGGCATGGTCACAGGTGAACCGCACATGCC
CGTCCGCCACACCTGGGGCGAGGTCCATGAGCGTGCTCGTTGCATCGCGGGCGGCCTGGCCGCCGCGGGTGTCGGTCTTG
GTGACGTTGTTGGGGTGCTGGCCGGCTTCCCGGTGGAGATCGCCCCCACGGCGCAGGCCCTGTGGATGCGCGGGGCCAGC
CTGACCATGCTGCACCAGCCCACACCGCGCACCGACTTGGCCGTGTGGGCCGAGGACACCATGACCGTCATCGGCATGAT
CGAGGCCAAGGCCGTGATCGTCTCCGAGCCCTTCCTCGTGGCCATTCCCATCCTTGAGCAGAAAGGCATGCAGGTCCTTA
CCGTCGCTGACCTTTTGGCGTCGGATCCGATCGGCCCCATCGAGGTCGGCGAGGACGACCTGGCGTTGATGCAGCTGACG
TCCGGATCTACCGGCTCCCCTAAAGCCGTCCAGATCACCCACCGCAACATCTACTCCAACGCCGAGGCAATGTTCGTCGG
CGCCCAGTATGACGTCGACAAGGACGTCATGGTCAGCTGGTTGCCCTGCTTCCATGACATGGGCATGGTGGGCTTCTTGA
CTATCCCGATGTTCTTCGGTGCGGAGCTGGTCAAGGTCACGCCAATGGACTTCCTGCGCGACACGCTGCTGTGGGCGAAG
CTCATCGACAAGTACCAGGGCACCATGACCGCGGCGCCCAACTTCGCCTACGCGCTGCTCGCCAAGCGGTTGCGGCGCCA
GGCCAAGCCCGGCGACTTCGATCTGTCGACCCTACGCTTCGCGCTGTCCGGCGCCGAGCCCGTCGAACCCGCCGACGTCG
AGGACCTGCTCGACGCGGGCAAGCCGTTCGGCCTGAGGCCCTCAGCGATCCTGCCGGCCTACGGCATGGCCGAGACCACG
CTGGCGGTGTCCTTCTCGGAGTGCAACGCCGGCCTCGTCGTGGACGAGGTTGACGCCGACCTGCTGGCGGCTCTGCGCCG
GGCCGTTCCCGCCACCAAAGGCAATACCCGCAGGCTGGCCACGCTAGGTCCGCTGCTGCAGGACCTAGAGGCCCGCATCA
TCGACGAACAGGGCGATGTCATGCCCGCCCGCGGCGTGGGTGTCATCGAGCTGCGCGGCGAGTCGCTAACTCCCGGCTAC
CTGACTATGGGTGGCTTCATCCCGGCCCAAGACGAGCATGGCTGGTACGACACGGGCGACCTCGGCTACCTCACCGAGGA
GGGCCACGTGGTGGTATGTGGCCGCGTCAAGGATGTCATCATCATGGCCGGGCGCAATATTTACCCGACCGACATCGAGC
GGGCGGCCGGCCGCGTCGACGGCGTTCGTCCGGGTTGCGCGGTGGCCGTGCGTCTCGATGCCGGACATTCGCGCGAATCC
TTTGCCGTCGCGGTCGAGTCGAACGCCTTCGAGGATCCCGCCGAGGTTCGTCGCATCGAGCATCAAGTGGCCCACGAGGT
GGTTGCCGAGGTCGACGTGCGGCCTCGCAACGTCGTGGTTCTTGGACCCGGGACCATTCCGAAGACGCCGTCGGGCAAGC
TGCGTCGGGCCAACTCCGTCACCCTGGTCACCTAA

Upstream 100 bases:

>100_bases
CTCCCCAACTGTGTGCGCGCGCCTGTCGCGATGTGGCATCCGGTAGGCGGACCATGAAAACCCGGACCTTGGGGACAGCA
CCGGAACCGAGGAGGTTGCC

Downstream 100 bases:

>100_bases
GGCCGCCGAGCAGACGCAAAATCCCCTCGACACGCCGGTTGCGAGGGGATTTTGCGTCTGCTCACGCGGGTCGTTACCAG
GCGTGGACGCGGTTTTGTGC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSRFTEKMFHNARTATTGMVTGEPHMPVRHTWGEVHERARCIAGGLAAAGVGLGDVVGVLAGFPVEIAPTAQALWMRGAS
LTMLHQPTPRTDLAVWAEDTMTVIGMIEAKAVIVSEPFLVAIPILEQKGMQVLTVADLLASDPIGPIEVGEDDLALMQLT
SGSTGSPKAVQITHRNIYSNAEAMFVGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMFFGAELVKVTPMDFLRDTLLWAK
LIDKYQGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAGKPFGLRPSAILPAYGMAETT
LAVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLATLGPLLQDLEARIIDEQGDVMPARGVGVIELRGESLTPGY
LTMGGFIPAQDEHGWYDTGDLGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRES
FAVAVESNAFEDPAEVRRIEHQVAHEVVAEVDVRPRNVVVLGPGTIPKTPSGKLRRANSVTLVT

Sequences:

>Translated_544_residues
MSRFTEKMFHNARTATTGMVTGEPHMPVRHTWGEVHERARCIAGGLAAAGVGLGDVVGVLAGFPVEIAPTAQALWMRGAS
LTMLHQPTPRTDLAVWAEDTMTVIGMIEAKAVIVSEPFLVAIPILEQKGMQVLTVADLLASDPIGPIEVGEDDLALMQLT
SGSTGSPKAVQITHRNIYSNAEAMFVGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMFFGAELVKVTPMDFLRDTLLWAK
LIDKYQGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAGKPFGLRPSAILPAYGMAETT
LAVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLATLGPLLQDLEARIIDEQGDVMPARGVGVIELRGESLTPGY
LTMGGFIPAQDEHGWYDTGDLGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRES
FAVAVESNAFEDPAEVRRIEHQVAHEVVAEVDVRPRNVVVLGPGTIPKTPSGKLRRANSVTLVT
>Mature_543_residues
SRFTEKMFHNARTATTGMVTGEPHMPVRHTWGEVHERARCIAGGLAAAGVGLGDVVGVLAGFPVEIAPTAQALWMRGASL
TMLHQPTPRTDLAVWAEDTMTVIGMIEAKAVIVSEPFLVAIPILEQKGMQVLTVADLLASDPIGPIEVGEDDLALMQLTS
GSTGSPKAVQITHRNIYSNAEAMFVGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMFFGAELVKVTPMDFLRDTLLWAKL
IDKYQGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAGKPFGLRPSAILPAYGMAETTL
AVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLATLGPLLQDLEARIIDEQGDVMPARGVGVIELRGESLTPGYL
TMGGFIPAQDEHGWYDTGDLGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRESF
AVAVESNAFEDPAEVRRIEHQVAHEVVAEVDVRPRNVVVLGPGTIPKTPSGKLRRANSVTLVT

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family

Homologues:

Organism=Homo sapiens, GI156151445, Length=310, Percent_Identity=25.1612903225806, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1788107, Length=345, Percent_Identity=26.9565217391304, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789201, Length=390, Percent_Identity=26.4102564102564, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693145, Length=534, Percent_Identity=24.1573033707865, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560308, Length=394, Percent_Identity=25.3807106598985, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=395, Percent_Identity=25.0632911392405, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI32563687, Length=382, Percent_Identity=25.130890052356, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=317, Percent_Identity=24.9211356466877, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=317, Percent_Identity=24.9211356466877, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=321, Percent_Identity=23.98753894081, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=358, Percent_Identity=24.3016759776536, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=400, Percent_Identity=23.75, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=330, Percent_Identity=25.4545454545455, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21355181, Length=404, Percent_Identity=24.7524752475248, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18859661, Length=443, Percent_Identity=26.1851015801354, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581924, Length=308, Percent_Identity=24.025974025974, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20130357, Length=511, Percent_Identity=23.8747553816047, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1013_MYCTU (O05598)

Other databases:

- EMBL:   BX842575
- PIR:   H70603
- RefSeq:   NP_215529.1
- ProteinModelPortal:   O05598
- EnsemblBacteria:   EBMYCT00000003128
- GeneID:   886035
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv1013
- TubercuList:   Rv1013
- GeneTree:   EBGT00050000015137
- HOGENOM:   HBG547964
- OMA:   VIIMGGR
- ProtClustDB:   PRK07768
- InterPro:   IPR020845
- InterPro:   IPR000873

Pfam domain/function: PF00501 AMP-binding

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58468; Mature: 58337

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFTEKMFHNARTATTGMVTGEPHMPVRHTWGEVHERARCIAGGLAAAGVGLGDVVGVL
CCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
AGFPVEIAPTAQALWMRGASLTMLHQPTPRTDLAVWAEDTMTVIGMIEAKAVIVSEPFLV
HCCCEEECCCHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEEEEEEEEEEEEEECCCEE
AIPILEQKGMQVLTVADLLASDPIGPIEVGEDDLALMQLTSGSTGSPKAVQITHRNIYSN
EEEECCCCCCEEEEHHHHHHCCCCCCCEECCCCEEEEEEECCCCCCCCEEEEEECHHHCC
AEAMFVGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMFFGAELVKVTPMDFLRDTLLWAK
CCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
LIDKYQGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAG
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCHHHHHHCC
KPFGLRPSAILPAYGMAETTLAVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEHH
TLGPLLQDLEARIIDEQGDVMPARGVGVIELRGESLTPGYLTMGGFIPAQDEHGWYDTGD
HHHHHHHHHHHHEECCCCCEECCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCC
LGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRES
CCEEECCCCEEEECCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCC
FAVAVESNAFEDPAEVRRIEHQVAHEVVAEVDVRPRNVVVLGPGTIPKTPSGKLRRANSV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCE
TLVT
EEEC
>Mature Secondary Structure 
SRFTEKMFHNARTATTGMVTGEPHMPVRHTWGEVHERARCIAGGLAAAGVGLGDVVGVL
CHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHH
AGFPVEIAPTAQALWMRGASLTMLHQPTPRTDLAVWAEDTMTVIGMIEAKAVIVSEPFLV
HCCCEEECCCHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEEEEEEEEEEEEEECCCEE
AIPILEQKGMQVLTVADLLASDPIGPIEVGEDDLALMQLTSGSTGSPKAVQITHRNIYSN
EEEECCCCCCEEEEHHHHHHCCCCCCCEECCCCEEEEEEECCCCCCCCEEEEEECHHHCC
AEAMFVGAQYDVDKDVMVSWLPCFHDMGMVGFLTIPMFFGAELVKVTPMDFLRDTLLWAK
CCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHH
LIDKYQGTMTAAPNFAYALLAKRLRRQAKPGDFDLSTLRFALSGAEPVEPADVEDLLDAG
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHEECCCCCCCCCCHHHHHHCC
KPFGLRPSAILPAYGMAETTLAVSFSECNAGLVVDEVDADLLAALRRAVPATKGNTRRLA
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEHH
TLGPLLQDLEARIIDEQGDVMPARGVGVIELRGESLTPGYLTMGGFIPAQDEHGWYDTGD
HHHHHHHHHHHHEECCCCCEECCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCC
LGYLTEEGHVVVCGRVKDVIIMAGRNIYPTDIERAAGRVDGVRPGCAVAVRLDAGHSRES
CCEEECCCCEEEECCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCC
FAVAVESNAFEDPAEVRRIEHQVAHEVVAEVDVRPRNVVVLGPGTIPKTPSGKLRRANSV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCE
TLVT
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230