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The map label for this gene is asnB [H]

Identifier: 121611749

GI number: 121611749

Start: 5359631

End: 5361385

Strand: Direct

Name: asnB [H]

Synonym: Veis_4847

Alternate gene names: 121611749

Gene position: 5359631-5361385 (Clockwise)

Preceding gene: 121611748

Following gene: 121611750

Centisome position: 96.28

GC content: 61.37

Gene sequence:

>1755_bases
ATGTGTGGGATAGCCGGTGTGGTCAACGCGACGGGGGTGACGAAAGACGATGTCTGGGCGATGGTCGGGAGCATCAGATA
CCGCGGCGTCGATGCACAAGCTGTCGAAGACCTGGGCGGTGCCATTGTCGGCAATGTCCTGCTGAAGATAGCCGCTCTCG
AAAACGGCAGGCAACCGATGTCGAGCACCGACGGCAAGGTCTGGGTTGTCTTCAACGGCGAGATCTTCAATTTCATCGAG
TTGCGCGAGCAGCTACAGGCCAAGGGCTACCGGTTCAAATCGCGCTGCGATACCGAGGTGCTGGTGCATCTGTGGTGCGA
AAAGGGCGAACAGATGCTCGACGATCTGGTCGGCATGTTCGCCTTTTTCATCTGGGACCAGCGCACGCAGACCGGCATGC
TGGCCCGCGACCGCCAGGGCATCAAGCCCTGCTTTTATGCGCCGTATCGCGGCGGCCTGGCGTTTGCCAGCGAGATCAAA
GCCATCCTGGCGCTGCCAAAGTTCGAGCGCAAAGTCAACGAATCGGCGTTGGGCGATGTGTTCAGCTTCAACTACTGCCC
ACCGCCGCAGACCTGCTTCGAAGGCATCCATCATCTGCCGCCGGGCTGTTTCATGCGTTACGGATCGGGCCGATTTTCCG
AGCCTGTGCGCTACTGGCGCTGGCCGCTCGATGGCGAGCGCGTCGACGCCTCGCAGGCCGATCTGGAAAATGCGCTCGGC
GAAGCCGTCAAGCTGCAAATGCGTTTTGAGGTCGATGGGGGGCTGTTTCTTTCGGGGGGCGTCGACTCTTCGGTCATCGC
CCATCACTTGCTGCCCCAGTGGAAGCAGCCCCGGTTGGACGCCATCGGTCTGCGCATCGAAGAGCAAGGTTTTTCCGAAT
ACGCTTACGCCGAGCGTGTGGCCACCGACCTGAACATCAACCTGAGCGCACTGGATATCCGACCGGCCGATATTGCGGAC
ATTGCACGCTCGGTCGTGCGGCATGCCGAGCAGCCGCATGGCGACTTTTCGTTTTTCCTGTTCTACCTGCTGTCGCGCCG
GGCGCACCAGATGGGCAAGGTCGTCATGTTCACGGGCGACGGCCCGGACGAGGTCATGCTGGGCTATCGCCACAACGCGC
AATTTTTCTCCGGGATGACGCGCGCCCGGTTTTCGATGCGCGACTACTTCGACCTCATCAGCTACAGCACCCGGCAAGAG
CGCCAGCGGGTGATGAGCCCGTCTTTTCTGCCCCACACCGAAGGCGCGTTCGACAGGTTCATGGGCATCATCGAACCCTT
TGGCGGCTTGGAGCCGATGGAACAGGTCGCCGCCTACGAGTTGACCTCGCTGATGCCTGGCAACGACTCCCCCAAGAGCG
ATCGCATGGGCGCCTGCTGGTCGATCGAAGGGCGCGCGCCCTTCCTGGACCACAGAGTCAGCGAATTGTTCGCGCGTTTG
CCGATCCAGTCCAAGTTCCACCAAGGGGTGGGCAAGCATTTCCTCAAGCGGGTGGCGCAGAGCTATTACGACCACGACTT
CGTGTTCCGGCCCAAGACCATGCCCACGCTACCGATCGGCGAGTGGATCAAAGGGCCGCTTTACCCATGGGCGCGCGACA
TCCTGGCGCTACCCGATGGCGGCCGCTTCGATGCCAGCGGGCTGCAACACATGTTGCAAGAGCACCGCAGCGGCATGCAC
AACCACACCAAGCCCCTGCGCACGCTGCTGATGACCAAACTGTGGCTGCAAGAGTTTTTCTCCGAGGGCGCTTGA

Upstream 100 bases:

>100_bases
TTGCGGTTGATCTACGAAGACCCCACGGGCAAGGCGCTGCGCGCGAACGCCCCGGAGCCGCAAGACTGCACGAACCGATG
AAAGACTTGAGGGGATCGCC

Downstream 100 bases:

>100_bases
AGCGAGCAGCGGGCGGGAACCCTAGTGTCGCGTCACCGATCAGATGTCGTAGGCTGCGCGCAGCCAATACCGAGCGTATT
GGCAAGCGATGCAACGCCGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MCGIAGVVNATGVTKDDVWAMVGSIRYRGVDAQAVEDLGGAIVGNVLLKIAALENGRQPMSSTDGKVWVVFNGEIFNFIE
LREQLQAKGYRFKSRCDTEVLVHLWCEKGEQMLDDLVGMFAFFIWDQRTQTGMLARDRQGIKPCFYAPYRGGLAFASEIK
AILALPKFERKVNESALGDVFSFNYCPPPQTCFEGIHHLPPGCFMRYGSGRFSEPVRYWRWPLDGERVDASQADLENALG
EAVKLQMRFEVDGGLFLSGGVDSSVIAHHLLPQWKQPRLDAIGLRIEEQGFSEYAYAERVATDLNINLSALDIRPADIAD
IARSVVRHAEQPHGDFSFFLFYLLSRRAHQMGKVVMFTGDGPDEVMLGYRHNAQFFSGMTRARFSMRDYFDLISYSTRQE
RQRVMSPSFLPHTEGAFDRFMGIIEPFGGLEPMEQVAAYELTSLMPGNDSPKSDRMGACWSIEGRAPFLDHRVSELFARL
PIQSKFHQGVGKHFLKRVAQSYYDHDFVFRPKTMPTLPIGEWIKGPLYPWARDILALPDGGRFDASGLQHMLQEHRSGMH
NHTKPLRTLLMTKLWLQEFFSEGA

Sequences:

>Translated_584_residues
MCGIAGVVNATGVTKDDVWAMVGSIRYRGVDAQAVEDLGGAIVGNVLLKIAALENGRQPMSSTDGKVWVVFNGEIFNFIE
LREQLQAKGYRFKSRCDTEVLVHLWCEKGEQMLDDLVGMFAFFIWDQRTQTGMLARDRQGIKPCFYAPYRGGLAFASEIK
AILALPKFERKVNESALGDVFSFNYCPPPQTCFEGIHHLPPGCFMRYGSGRFSEPVRYWRWPLDGERVDASQADLENALG
EAVKLQMRFEVDGGLFLSGGVDSSVIAHHLLPQWKQPRLDAIGLRIEEQGFSEYAYAERVATDLNINLSALDIRPADIAD
IARSVVRHAEQPHGDFSFFLFYLLSRRAHQMGKVVMFTGDGPDEVMLGYRHNAQFFSGMTRARFSMRDYFDLISYSTRQE
RQRVMSPSFLPHTEGAFDRFMGIIEPFGGLEPMEQVAAYELTSLMPGNDSPKSDRMGACWSIEGRAPFLDHRVSELFARL
PIQSKFHQGVGKHFLKRVAQSYYDHDFVFRPKTMPTLPIGEWIKGPLYPWARDILALPDGGRFDASGLQHMLQEHRSGMH
NHTKPLRTLLMTKLWLQEFFSEGA
>Mature_584_residues
MCGIAGVVNATGVTKDDVWAMVGSIRYRGVDAQAVEDLGGAIVGNVLLKIAALENGRQPMSSTDGKVWVVFNGEIFNFIE
LREQLQAKGYRFKSRCDTEVLVHLWCEKGEQMLDDLVGMFAFFIWDQRTQTGMLARDRQGIKPCFYAPYRGGLAFASEIK
AILALPKFERKVNESALGDVFSFNYCPPPQTCFEGIHHLPPGCFMRYGSGRFSEPVRYWRWPLDGERVDASQADLENALG
EAVKLQMRFEVDGGLFLSGGVDSSVIAHHLLPQWKQPRLDAIGLRIEEQGFSEYAYAERVATDLNINLSALDIRPADIAD
IARSVVRHAEQPHGDFSFFLFYLLSRRAHQMGKVVMFTGDGPDEVMLGYRHNAQFFSGMTRARFSMRDYFDLISYSTRQE
RQRVMSPSFLPHTEGAFDRFMGIIEPFGGLEPMEQVAAYELTSLMPGNDSPKSDRMGACWSIEGRAPFLDHRVSELFARL
PIQSKFHQGVGKHFLKRVAQSYYDHDFVFRPKTMPTLPIGEWIKGPLYPWARDILALPDGGRFDASGLQHMLQEHRSGMH
NHTKPLRTLLMTKLWLQEFFSEGA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=395, Percent_Identity=28.3544303797468, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321563, Length=414, Percent_Identity=25.8454106280193, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6325403, Length=420, Percent_Identity=24.2857142857143, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45553209, Length=401, Percent_Identity=25.4364089775561, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 66100; Mature: 66100

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGVVNATGVTKDDVWAMVGSIRYRGVDAQAVEDLGGAIVGNVLLKIAALENGRQPM
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SSTDGKVWVVFNGEIFNFIELREQLQAKGYRFKSRCDTEVLVHLWCEKGEQMLDDLVGMF
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AFFIWDQRTQTGMLARDRQGIKPCFYAPYRGGLAFASEIKAILALPKFERKVNESALGDV
HHHHHCCCCCCCCHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHCHHHHHHHCHHHHHHH
FSFNYCPPPQTCFEGIHHLPPGCFMRYGSGRFSEPVRYWRWPLDGERVDASQADLENALG
HCCCCCCCHHHHHHHHHHCCCHHHEECCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHH
EAVKLQMRFEVDGGLFLSGGVDSSVIAHHLLPQWKQPRLDAIGLRIEEQGFSEYAYAERV
HHEEEEEEEEECCCEEEECCCCHHHHHHHHCCCCCCCCCCHHCCEEECCCCHHHHHHHHH
ATDLNINLSALDIRPADIADIARSVVRHAEQPHGDFSFFLFYLLSRRAHQMGKVVMFTGD
HHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECC
GPDEVMLGYRHNAQFFSGMTRARFSMRDYFDLISYSTRQERQRVMSPSFLPHTEGAFDRF
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
MGIIEPFGGLEPMEQVAAYELTSLMPGNDSPKSDRMGACWSIEGRAPFLDHRVSELFARL
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHC
PIQSKFHQGVGKHFLKRVAQSYYDHDFVFRPKTMPTLPIGEWIKGPLYPWARDILALPDG
CCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHCCCCCHHHHHHEECCCC
GRFDASGLQHMLQEHRSGMHNHTKPLRTLLMTKLWLQEFFSEGA
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGIAGVVNATGVTKDDVWAMVGSIRYRGVDAQAVEDLGGAIVGNVLLKIAALENGRQPM
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SSTDGKVWVVFNGEIFNFIELREQLQAKGYRFKSRCDTEVLVHLWCEKGEQMLDDLVGMF
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AFFIWDQRTQTGMLARDRQGIKPCFYAPYRGGLAFASEIKAILALPKFERKVNESALGDV
HHHHHCCCCCCCCHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHCHHHHHHHCHHHHHHH
FSFNYCPPPQTCFEGIHHLPPGCFMRYGSGRFSEPVRYWRWPLDGERVDASQADLENALG
HCCCCCCCHHHHHHHHHHCCCHHHEECCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHH
EAVKLQMRFEVDGGLFLSGGVDSSVIAHHLLPQWKQPRLDAIGLRIEEQGFSEYAYAERV
HHEEEEEEEEECCCEEEECCCCHHHHHHHHCCCCCCCCCCHHCCEEECCCCHHHHHHHHH
ATDLNINLSALDIRPADIADIARSVVRHAEQPHGDFSFFLFYLLSRRAHQMGKVVMFTGD
HHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECC
GPDEVMLGYRHNAQFFSGMTRARFSMRDYFDLISYSTRQERQRVMSPSFLPHTEGAFDRF
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
MGIIEPFGGLEPMEQVAAYELTSLMPGNDSPKSDRMGACWSIEGRAPFLDHRVSELFARL
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHC
PIQSKFHQGVGKHFLKRVAQSYYDHDFVFRPKTMPTLPIGEWIKGPLYPWARDILALPDG
CCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHCCCCCHHHHHHEECCCC
GRFDASGLQHMLQEHRSGMHNHTKPLRTLLMTKLWLQEFFSEGA
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]