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The map label for this gene is ribH

Identifier: 121611695

GI number: 121611695

Start: 5292723

End: 5293187

Strand: Direct

Name: ribH

Synonym: Veis_4792

Alternate gene names: 121611695

Gene position: 5292723-5293187 (Clockwise)

Preceding gene: 121611694

Following gene: 121611696

Centisome position: 95.08

GC content: 65.59

Gene sequence:

>465_bases
ATGTTCGGCGCAGACAAAGGCACGGCAGACCGGCTCGACGGCAAGAAACTGCACATCGGCATCGTGCAAGCCCGCTTCAA
CAGCGGCATCACCAACGCGCTGGCAGCGGCCTGCTGCGCCGAACTGCTGGCGCTGGGCGTGCAAGACAAGCACATCATGC
AGGTGCAGGTGCCCGGCGCGCTCGAAATCCCCGTGGCCCTGCAAGCCATGGCCGAGCGCAACCACTACGACGCGCTGATC
GCGCTGGGCTGCATCATCCGTGGCCAGACCTACCACTTCGAGCTGGTGGCCAACGAATCGGGCGCCGGCGTGACGCGGCT
GGCGCTCGACTACCAAATCCCGATCGCCAACGCCATCATCACCGTCGAGAACCTCGAGCAGGCCATTGCCCGCCAGAGCG
AAAAGGGCGCCGACGCCGCCCGCGTGGCGGTGGAAATGGCCAATTTGCTGGACGCGCTGTCATGA

Upstream 100 bases:

>100_bases
GCGGCGTGCACCGGATGGCGCTGATGGGCCAACCCCGGCGCATGCCGAGCATGGCCGGCTACGGGCTGGAGATCACCGGC
TTCATCCCCAAGGAATGAAC

Downstream 100 bases:

>100_bases
GCACGAACCACGACGCCGCGCCGGCTGACGCTCGACCGCCCCCGGCAGCGCGCGCCAAGGCCATCGGCGGGCGCGGGCCG
GGCGCGCGCCTGCCCCGCAG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MFGADKGTADRLDGKKLHIGIVQARFNSGITNALAAACCAELLALGVQDKHIMQVQVPGALEIPVALQAMAERNHYDALI
ALGCIIRGQTYHFELVANESGAGVTRLALDYQIPIANAIITVENLEQAIARQSEKGADAARVAVEMANLLDALS

Sequences:

>Translated_154_residues
MFGADKGTADRLDGKKLHIGIVQARFNSGITNALAAACCAELLALGVQDKHIMQVQVPGALEIPVALQAMAERNHYDALI
ALGCIIRGQTYHFELVANESGAGVTRLALDYQIPIANAIITVENLEQAIARQSEKGADAARVAVEMANLLDALS
>Mature_154_residues
MFGADKGTADRLDGKKLHIGIVQARFNSGITNALAAACCAELLALGVQDKHIMQVQVPGALEIPVALQAMAERNHYDALI
ALGCIIRGQTYHFELVANESGAGVTRLALDYQIPIANAIITVENLEQAIARQSEKGADAARVAVEMANLLDALS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=141, Percent_Identity=43.9716312056738, Blast_Score=109, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324429, Length=155, Percent_Identity=30.3225806451613, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_VEREI (A1WS77)

Other databases:

- EMBL:   CP000542
- RefSeq:   YP_999502.1
- ProteinModelPortal:   A1WS77
- SMR:   A1WS77
- STRING:   A1WS77
- GeneID:   4694008
- GenomeReviews:   CP000542_GR
- KEGG:   vei:Veis_4792
- NMPDR:   fig|391735.5.peg.4396
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   NRAGIKA
- PhylomeDB:   A1WS77
- ProtClustDB:   PRK00061
- BioCyc:   VEIS391735:VEIS_4792-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16307; Mature: 16307

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGADKGTADRLDGKKLHIGIVQARFNSGITNALAAACCAELLALGVQDKHIMQVQVPGA
CCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
LEIPVALQAMAERNHYDALIALGCIIRGQTYHFELVANESGAGVTRLALDYQIPIANAII
EECHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEEECCHHHEEE
TVENLEQAIARQSEKGADAARVAVEMANLLDALS
EHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGADKGTADRLDGKKLHIGIVQARFNSGITNALAAACCAELLALGVQDKHIMQVQVPGA
CCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
LEIPVALQAMAERNHYDALIALGCIIRGQTYHFELVANESGAGVTRLALDYQIPIANAII
EECHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEEECCHHHEEE
TVENLEQAIARQSEKGADAARVAVEMANLLDALS
EHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA