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The map label for this gene is leuD [H]

Identifier: 121611564

GI number: 121611564

Start: 5148504

End: 5149106

Strand: Direct

Name: leuD [H]

Synonym: Veis_4656

Alternate gene names: 121611564

Gene position: 5148504-5149106 (Clockwise)

Preceding gene: 121611563

Following gene: 121611565

Centisome position: 92.49

GC content: 54.06

Gene sequence:

>603_bases
ATGGCACGAGCCAACATCGACACGGATGCGATTCTTCCGAAGCAATTCATGAAGTCGATTGAGCGCACAGGCTTCGGAGT
TCACCTGTTCGATGGTTTTCGATACCTCGATGCTGGCTCGCTTGGCATGGACTGCAGTCGCCGTCCTGAGAATCCCGATT
TCATCCTTAACTGGCCTCGTTATCGCGAGGCGTCAATTTTGCTCGCGGGGGCCAACTTCGGTTGCGGGTCCAGTCGCGAG
CACGCGCCATGGGCTATCGAGCAATACGGATTCCGTGTGCTGATTGCTTCGAGTTTTGCTGACATCTTCCGCGACAACTG
CTTCAAAAATGGACTCTTGCCAATCGTCCTTGCTAGCGAAACGATGGACAGACTTTTCGATGAGGTGAGCCACCAAGTCG
GCTTCGAACTCGAAGTCGATCTCCCACGGCAGACGATCTCCTGTCCTGCGGGATGGTGCATTGAGTTCGAAATGGACTCC
ATCCGCAAAGCGCATCTCCTGGAGGGAATCGATGAAATCGGATCCACCTTGTCGCTCTCGAAAAAAATAGGCGACTTCGA
ACGTGCTCGGCGCCAGTCGCATCCTTGGTTCTTTGGTGCATGA

Upstream 100 bases:

>100_bases
AACAGCACATTGACGCAGTTGGTGAGGGTCAAGGAACGTCAGTTTCGAAAGTAGCGCACCTATGAAGTCCTTCGTCCTTC
ACCGAGGTCTCGCGGTCCCG

Downstream 100 bases:

>100_bases
GGTCCATACAGCCGAATCGTTGCCAGATATGAATAACTTCCTACGTATAGAACGCTACACATGATGAATCAAGAAAAATT
CTTAACGGCGCGACGGCGGA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MARANIDTDAILPKQFMKSIERTGFGVHLFDGFRYLDAGSLGMDCSRRPENPDFILNWPRYREASILLAGANFGCGSSRE
HAPWAIEQYGFRVLIASSFADIFRDNCFKNGLLPIVLASETMDRLFDEVSHQVGFELEVDLPRQTISCPAGWCIEFEMDS
IRKAHLLEGIDEIGSTLSLSKKIGDFERARRQSHPWFFGA

Sequences:

>Translated_200_residues
MARANIDTDAILPKQFMKSIERTGFGVHLFDGFRYLDAGSLGMDCSRRPENPDFILNWPRYREASILLAGANFGCGSSRE
HAPWAIEQYGFRVLIASSFADIFRDNCFKNGLLPIVLASETMDRLFDEVSHQVGFELEVDLPRQTISCPAGWCIEFEMDS
IRKAHLLEGIDEIGSTLSLSKKIGDFERARRQSHPWFFGA
>Mature_199_residues
ARANIDTDAILPKQFMKSIERTGFGVHLFDGFRYLDAGSLGMDCSRRPENPDFILNWPRYREASILLAGANFGCGSSREH
APWAIEQYGFRVLIASSFADIFRDNCFKNGLLPIVLASETMDRLFDEVSHQVGFELEVDLPRQTISCPAGWCIEFEMDSI
RKAHLLEGIDEIGSTLSLSKKIGDFERARRQSHPWFFGA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=184, Percent_Identity=48.9130434782609, Blast_Score=179, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321429, Length=203, Percent_Identity=42.3645320197044, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22629; Mature: 22498

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARANIDTDAILPKQFMKSIERTGFGVHLFDGFRYLDAGSLGMDCSRRPENPDFILNWPR
CCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCCCCCEEEECCC
YREASILLAGANFGCGSSREHAPWAIEQYGFRVLIASSFADIFRDNCFKNGLLPIVLASE
CCCCEEEEEECCCCCCCCCCCCCEEHHHCCEEEEEHHHHHHHHHHHHHCCCCEEEEECHH
TMDRLFDEVSHQVGFELEVDLPRQTISCPAGWCIEFEMDSIRKAHLLEGIDEIGSTLSLS
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
KKIGDFERARRQSHPWFFGA
HHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure 
ARANIDTDAILPKQFMKSIERTGFGVHLFDGFRYLDAGSLGMDCSRRPENPDFILNWPR
CCCCCCCCCCCHHHHHHHHHHCCCEEEEECCEEEECCCCCCCCCCCCCCCCCEEEECCC
YREASILLAGANFGCGSSREHAPWAIEQYGFRVLIASSFADIFRDNCFKNGLLPIVLASE
CCCCEEEEEECCCCCCCCCCCCCEEHHHCCEEEEEHHHHHHHHHHHHHCCCCEEEEECHH
TMDRLFDEVSHQVGFELEVDLPRQTISCPAGWCIEFEMDSIRKAHLLEGIDEIGSTLSLS
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
KKIGDFERARRQSHPWFFGA
HHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA